公开(公告)号：US07037669B2

公开(公告)日：2006-05-02

申请号：US10805813

申请日：2004-03-22

申请人： Yi Feng Zheng ,  Khaled A. Yamout ,  Donald E. Berger, Jr. ,  Mae W. Hu ,  Hshiou-ting Liu

发明人： Yi Feng Zheng ,  Khaled A. Yamout ,  Donald E. Berger, Jr. ,  Mae W. Hu ,  Hshiou-ting Liu

IPC分类号： G01N33/53 ,  G01N33/531 ,  C07K16/00 ,  C07K17/02 ,  C07D317/58

CPC分类号： C12N9/0006 ,  G01N33/946 ,  Y10S435/961 ,  Y10S435/975

摘要： Methods, compositions and kits are disclosed. Enzyme conjugates of Formula I may be employed in assays for the determination of an amphetamine and/or a methamphetamine. Immunogenic conjugates of Formula I may be employed to prepare antibodies for an amphetamine and/or for a methamphetamine for use in assays for the determination of an amphetamine and/or a methamphetamine. The enzyme conjugates may also be employed to screen antibodies for use in such methods.

摘要翻译： 公开了方法，组合物和试剂盒。 式I的酶缀合物可以用于测定苯丙胺和/或甲基苯丙胺的测定中。 式I的免疫原性缀合物可用于制备安非他明和/或甲基苯丙胺的抗体，用于测定苯丙胺和/或甲基苯丙胺。 酶缀合物也可用于筛选用于这些方法的抗体。

公开(公告)号：US07115383B2

公开(公告)日：2006-10-03

申请号：US10806327

申请日：2004-03-22

申请人： Yi Feng Zheng ,  Richard F. Parrish ,  Johnny Valdez ,  Hshiou-ting Liu

发明人： Yi Feng Zheng ,  Richard F. Parrish ,  Johnny Valdez ,  Hshiou-ting Liu

IPC分类号： G01N33/535 ,  G01N33/531 ,  C07K16/00 ,  C07K17/02

CPC分类号： G01N33/946 ,  Y10S435/961 ,  Y10S435/975

摘要： Methods, compositions and kits are disclosed. The compounds disclosed comprise an amphetamine moiety and a methamphetamine moiety linked together by a first linking group. A second linking group depends from the first linking group and comprises a functional group. The distance of the amphetamine moiety and the methamphetamine moiety from the point of linkage of the second linking group to the first linking group is approximately the same. The compounds may be linked to labels and used in assays for the detection of amphetamine and/or methamphetamine in samples suspected of containing these drugs.

摘要翻译： 公开了方法，组合物和试剂盒。 所公开的化合物包括通过第一连接基团连接在一起的苯丙胺部分和甲基苯丙胺部分。 第二连接基团取决于第一连接基团并且包含官能团。 苯丙胺部分和甲基苯丙胺部分与第二连接基团与第一连接基团的连接点的距离大致相同。 化合物可以与标记物连接，并用于检测疑似含有这些药物的样品中苯丙胺和/或甲基苯丙胺的检测。

公开(公告)号：US07189582B2

公开(公告)日：2007-03-13

申请号：US11116928

申请日：2005-04-27

申请人： Jian Chen ,  Holger Keim ,  Hshiou-ting Liu ,  Yi Feng Zheng ,  Yali Yang ,  Cathy K. Worley

发明人： Jian Chen ,  Holger Keim ,  Hshiou-ting Liu ,  Yi Feng Zheng ,  Yali Yang ,  Cathy K. Worley

IPC分类号： G01N33/532 ,  C07K16/44 ,  C07K1/13 ,  C07D267/00

CPC分类号： C07K16/44 ,  A61K47/61 ,  A61K47/643 ,  A61K47/646 ,  A61K47/6923 ,  C07D498/18 ,  C07D519/00 ,  G01N33/9493 ,  Y10S436/815 ,  Y10S530/807 ,  Y10T436/13

摘要： Compounds are disclosed comprising a moiety, such as a poly(amino acid), a non-poly(amino acid) label moiety, or a non-poly(amino acid) immunogenic carrier, linked to a sirolimus compound at position 26 or at position 32. Such sirolimus compounds comprising an immunogenic carrier can be employed to raise both polyclonal and monoclonal antibodies to the sirolimus compound. Polyclonal antibodies are also disclosed, which are raised against a compound wherein a moiety, such as a poly(amino acid), a non-poly(amino acid) label moiety, or a non-poly(amino acid) immunogenic carrier, is linked to a sirolimus compound at position 32. The above antibodies and sirolimus compounds comprising a label can be used in assays for the detection of sirolimus compounds.

摘要翻译： 公开了包含部分，例如聚（氨基酸），非聚（氨基酸）标记部分或非聚（氨基酸）免疫原性载体的部分，其连接于位置26或位置的西罗莫司化合物 包含免疫原性载体的这种西罗莫司化合物可用于提高西罗莫司化合物的多克隆和单克隆抗体。 还公开了多克隆抗体，其针对其中部分例如聚（氨基酸），非聚（氨基酸）标记部分或非聚（氨基酸）免疫原性载体连接的化合物产生 至32位的西罗莫司化合物。包含标记的上述抗体和西罗莫司化合物可用于检测西罗莫司化合物。

公开(公告)号：US06991911B2

公开(公告)日：2006-01-31

申请号：US10736005

申请日：2003-12-15

申请人： Yi Feng Zheng ,  Hshiou-ting Liu

发明人： Yi Feng Zheng ,  Hshiou-ting Liu

IPC分类号： G01N33/53 ,  G01N33/531 ,  C07K16/00 ,  C07K17/02 ,  C07D317/58

CPC分类号： C07D405/12 ,  C07D317/58 ,  C07K16/44 ,  G01N33/946 ,  Y10S435/961 ,  Y10S435/975

摘要： Methods, compositions and kits are disclosed. The methods are directed to determining the presence of entactogen analytes such as, for example, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-methamphetamine (MDMA), 3,4-methylenedioxy-ethylamphetamine (MDEA) and 4-hydroxy-3-methoxy-methamphetamine (HMMA). The method comprises providing in combination in a medium (i) a sample suspected of containing the compound and (ii) an antibody raised against a compound of Formula I that comprises a protein. The medium is examined for the presence a complex comprising the compound and the antibody where the presence of such as complex indicates the presence of the compound in the sample. In one aspect of the above embodiment, the combination further comprises a label conjugate of the compound Formula I.

摘要翻译： 公开了方法，组合物和试剂盒。 该方法涉及确定活性成分分析物的存在，例如3,4-亚甲二氧基苯丙胺（MDA），3,4-亚甲二氧基 - 甲基苯丙胺（MDMA），3,4-亚甲二氧基 - 乙基苯丙胺（MDEA）和4- 羟基-3-甲氧基 - 甲基苯丙胺（HMMA）。 该方法包括在介质中提供（i）怀疑含有该化合物的样品和（ii）针对包含蛋白质的式I化合物产生的抗体。 检查介质是否存在包含化合物和抗体的复合物，其中诸如复合物的存在表明样品中存在化合物。 在上述实施方案的一个方面，组合还包含化合物式I的标记缀合物。

公开(公告)号：US20090087865A1

公开(公告)日：2009-04-02

申请号：US12239063

申请日：2008-09-26

申请人： Kenneth Cornell Kasper ,  Henry Jeong ,  Dariush Davalian ,  Hshiou-ting Liu ,  Paul Levi Miller ,  Denise Leah Williams

发明人： Kenneth Cornell Kasper ,  Henry Jeong ,  Dariush Davalian ,  Hshiou-ting Liu ,  Paul Levi Miller ,  Denise Leah Williams

IPC分类号： G01N33/53 ,  C07K16/18 ,  C12N9/00 ,  G01N33/566

CPC分类号： A61K51/1093 ,  C07K16/1292 ,  Y10S436/815

摘要： An IgG1λ monoclonal antibody to the immunosuppressive drug tacrolimus has improved properties. In particular, this monoclonal antibody, designated 1H6, has reduced cross-reactivity to several tacrolimus metabolites. This antibody is suitable for performance of immunoassays such as homogeneous immunoassays to detect or determine the presence or concentration of tacrolimus in samples such as blood samples. The invention further includes derivatives of tacrolimus derivatized at a non-binding portion of the molecule useful in immunizing antibody-producing animals and in producing such monoclonal antibodies, as well as labeled derivatives of tacrolimus useful as tacrolimus analogues in such assays. The invention further includes immunoassay methods for the detection of tacrolimus and test kits useful in performing such immunoassays.

摘要翻译： 针对免疫抑制药物他克莫司的IgG1λ单克隆抗体具有改善的性质。 特别地，称为1H6的单克隆抗体具有降低的几种他克莫司代谢物的交叉反应性。 该抗体适用于进行免疫测定，例如均匀免疫测定，以检测或确定样品如血液样品中他克莫司的存在或浓度。 本发明还包括在用于免疫产生抗体的动物和用于制备此类单克隆抗体的分子的非结合部分衍生的他克莫司的衍生物，以及在该测定中可用作他克莫司类似物的他克莫司的标记衍生物。 本发明还包括用于检测他克莫司的免疫测定方法和用于进行这种免疫测定的试剂盒。

公开(公告)号：US07078495B1

公开(公告)日：2006-07-18

申请号：US09368010

申请日：1999-08-03

申请人： Kenneth Cornell Kasper ,  Henry Jeong ,  Dariush Davalian ,  Hshiou-ting Liu ,  Paul Levi Miller ,  Denise Leah Williams

发明人： Kenneth Cornell Kasper ,  Henry Jeong ,  Dariush Davalian ,  Hshiou-ting Liu ,  Paul Levi Miller ,  Denise Leah Williams

IPC分类号： C07K16/44 ,  G01N33/533 ,  G01N33/535 ,  G01N33/546

CPC分类号： A61K51/1093 ,  C07K16/1292 ,  Y10S436/815

摘要： An IgG1 λ monoclonal antibody to the immunosuppressive drug tacrolimus has improved properties. In particular, this monoclonal antibody, designated 1H6, has reduced cross-reactivity to several tacrolimus metabolites. This antibody is suitable for performance of immunoassays such as homogeneous immunoassays to detect or determine the presence or concentration of tacrolimus in samples such as blood samples. The invention further includes derivatives of tacrolimus derivatized at a non-binding portion of the molecule useful in immunizing antibody-producing animals and in producing such monoclonal antibodies, as well as labeled derivatives of tacrolimus useful as tacrolimus analogues in such assays. The invention further includes immunoassay methods for the detection of tacrolimus and test kits useful in performing such immunoassays.

摘要翻译： 针对免疫抑制药物他克莫司的IgG1λ1λ单克隆抗体具有改善的性质。 特别地，称为1H6的单克隆抗体具有降低的几种他克莫司代谢物的交叉反应性。 该抗体适用于进行免疫测定，例如均匀免疫测定，以检测或确定样品如血液样品中他克莫司的存在或浓度。 本发明还包括在该分子的非结合部分衍生的他克莫司的衍生物，其可用于免疫产生抗体的动物和产生此类单克隆抗体，以及在该测定中用作他克莫司类似物的他克莫司的标记衍生物。 本发明还包括用于检测他克莫司的免疫测定方法和用于进行这种免疫测定的试剂盒。

公开(公告)号：US08030458B2

公开(公告)日：2011-10-04

申请号：US12239063

申请日：2008-09-26

申请人： Kenneth Cornell Kasper ,  Henry Jeong ,  Dariush Davalian ,  Hshiou-ting Liu ,  Paul Levi Miller ,  Denise Leah Williams

发明人： Kenneth Cornell Kasper ,  Henry Jeong ,  Dariush Davalian ,  Hshiou-ting Liu ,  Paul Levi Miller ,  Denise Leah Williams

IPC分类号： C07K16/00 ,  G01N33/53 ,  G01N21/76

CPC分类号： A61K51/1093 ,  C07K16/1292 ,  Y10S436/815

摘要： An IgG1λ monoclonal antibody to the immunosuppressive drug tacrolimus has improved properties. In particular, this monoclonal antibody, designated 1H6, has reduced cross-reactivity to several tacrolimus metabolites. This antibody is suitable for performance of immunoassays such as homogeneous immunoassays to detect or determine the presence or concentration of tacrolimus in samples such as blood samples. The invention further includes derivatives of tacrolimus derivatized at a non-binding portion of the molecule useful in immunizing antibody-producing animals and in producing such monoclonal antibodies, as well as labeled derivatives of tacrolimus useful as tacrolimus analogues in such assays. The invention further includes immunoassay methods for the detection of tacrolimus and test kits useful in performing such immunoassays.

摘要翻译： 针对免疫抑制药物他克莫司的IgG1λ单克隆抗体具有改善的性质。 特别地，称为1H6的单克隆抗体具有降低的几种他克莫司代谢物的交叉反应性。 该抗体适用于进行免疫测定，例如均匀免疫测定，以检测或确定样品如血液样品中他克莫司的存在或浓度。 本发明还包括在用于免疫产生抗体的动物和用于制备此类单克隆抗体的分子的非结合部分衍生的他克莫司的衍生物，以及在该测定中可用作他克莫司类似物的他克莫司的标记衍生物。 本发明还包括用于检测他克莫司的免疫测定方法和用于进行这种免疫测定的试剂盒。

公开(公告)号：US20050208604A1

公开(公告)日：2005-09-22

申请号：US10806327

申请日：2004-03-22

申请人： Yi Zheng ,  Richard Parrish ,  Johnny Valdez ,  Hshiou-ting Liu

发明人： Yi Zheng ,  Richard Parrish ,  Johnny Valdez ,  Hshiou-ting Liu

IPC分类号： C07C211/54 ,  C07K1/13 ,  C07K16/00 ,  C12N9/96 ,  G01N33/53 ,  G01N33/531 ,  G01N33/537 ,  G01N33/543 ,  G01N33/94

CPC分类号： G01N33/946 ,  Y10S435/961 ,  Y10S435/975

摘要： Methods, compositions and kits are disclosed. The compounds disclosed comprise an amphetamine moiety and a methamphetamine moiety linked together by a first linking group. A second linking group depends from the first linking group and comprises a functional group. The distance of the amphetamine moiety and the methamphetamine moiety from the point of linkage of the second linking group to the first linking group is approximately the same. The compounds may be linked to labels and used in assays for the detection of amphetamine and/or methamphetamine in samples suspected of containing these drugs.

摘要翻译： 公开了方法，组合物和试剂盒。 所公开的化合物包括通过第一连接基团连接在一起的苯丙胺部分和甲基苯丙胺部分。 第二连接基团取决于第一连接基团并且包含官能团。 苯丙胺部分和甲基苯丙胺部分与第二连接基团与第一连接基团的连接点的距离大致相同。 化合物可以与标记物连接，并用于检测疑似含有这些药物的样品中苯丙胺和/或甲基苯丙胺的检测。

公开(公告)号：US20060246518A1

公开(公告)日：2006-11-02

申请号：US11116928

申请日：2005-04-27

申请人： Jian Chen ,  Holger Keim ,  Hshiou-ting Liu ,  Yi Zheng ,  Yali Yang ,  Cathy Worley

发明人： Jian Chen ,  Holger Keim ,  Hshiou-ting Liu ,  Yi Zheng ,  Yali Yang ,  Cathy Worley

IPC分类号： G01N33/53 ,  C07K16/18 ,  C07K14/47 ,  C07D491/14

CPC分类号： C07K16/44 ,  A61K47/61 ,  A61K47/643 ,  A61K47/646 ,  A61K47/6923 ,  C07D498/18 ,  C07D519/00 ,  G01N33/9493 ,  Y10S436/815 ,  Y10S530/807 ,  Y10T436/13

摘要： Compounds are disclosed comprising a moiety, such as a poly(amino acid), a non-poly(amino acid) label moiety, or a non-poly(amino acid) immunogenic carrier, linked to a sirolimus compound at position 26 or at position 32. Such sirolimus compounds comprising an immunogenic carrier can be employed to raise both polyclonal and monoclonal antibodies to the sirolimus compound. Polyclonal antibodies are also disclosed, which are raised against a compound wherein a moiety, such as a poly(amino acid), a non-poly(amino acid) label moiety, or a non-poly(amino acid) immunogenic carrier, is linked to a sirolimus compound at position 32. The above antibodies and sirolimus compounds comprising a label can be used in assays for the detection of sirolimus compounds.

摘要翻译： 公开了包含部分，例如聚（氨基酸），非聚（氨基酸）标记部分或非聚（氨基酸）免疫原性载体的部分，其连接于位置26或位置的西罗莫司化合物 包含免疫原性载体的这种西罗莫司化合物可用于提高西罗莫司化合物的多克隆和单克隆抗体。 还公开了多克隆抗体，其针对其中部分例如聚（氨基酸），非聚（氨基酸）标记部分或非聚（氨基酸）免疫原性载体连接的化合物产生 至32位的西罗莫司化合物。包含标记的上述抗体和西罗莫司化合物可用于检测西罗莫司化合物。

公开(公告)号：US20060223998A1

公开(公告)日：2006-10-05

申请号：US11093676

申请日：2005-03-30

申请人： Fang-Jie Zhang ,  Hshiou-ting Liu

发明人： Fang-Jie Zhang ,  Hshiou-ting Liu

IPC分类号： C07D457/02

CPC分类号： C07D457/06

摘要： A general method for the synthesis of OH-LSD and its analogs has been successfully developed. This method is convenient and efficient and allows preparation of a series of OH-LSD analogs with linkers attached at various (C-8, N-6 or N-1) positions.

摘要翻译： 合成OH-LSD及其类似物的一般方法已经成功开发。 该方法方便高效，可以制备一系列具有连接在各种（C-8，N-6或N-1）位上的接头的OH-LSD类似物。