公开(公告)号：US20060247425A1

公开(公告)日：2006-11-02

申请号：US10555538

申请日：2005-05-31

申请人： Yong-Hoon Chung ,  Ki-Wan Yi ,  Hoon-Sik Cho ,  Hong-Gyu Park ,  Kwang-Seong Kim

发明人： Yong-Hoon Chung ,  Ki-Wan Yi ,  Hoon-Sik Cho ,  Hong-Gyu Park ,  Kwang-Seong Kim

IPC分类号： A61K39/395 ,  A61K39/00 ,  C07H21/04 ,  C12N5/06 ,  C12P21/04 ,  C07K16/46

CPC分类号： A61K38/1774 ,  C07K2317/21 ,  C07K2317/41 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/94 ,  C07K2319/30

摘要： The present invention relates to a glycosylated immunoglobulin or a fragment thereof, in which an immunoglobulin variant, comprising one or more amino acid modifications selected from the group consisting of M160N, A195N, T243N, E265N, Y299T, F331T and Q346N, is additionally glycosylated, and a gene encoding the same. Also, the present invention relates to a glycosylated fusion protein formed as a result of linkage of (a) a glycosylated immunoglobulin or a fragment thereof, in which an immunoglobulin variant having a modified amino acid sequence forming one or more Asn-X-Ser/Thr sequences is additionally glycosylated, with (b) at least one biologically active protein or a portion thereof, a gene encoding the same, a recombination expression vector comprising the gene, a host cell transformed or transfected with the recombinant expression vector, and a method of preparing a glycosylated fusion protein comprising culturing the transformant or transfectant and isolating the glycosylated fusion protein from the culture, and a pharmaceutical composition comprising the glycosylated fusion protein thus prepared.

摘要翻译： 本发明涉及糖基化免疫球蛋白或其片段，其中包含一个或多个选自M160N，A195N，T​​243N，E265N，Y299T，F331T和Q346N的氨基酸修饰的免疫球蛋白变体另外被糖基化， 和编码该基因的基因。 此外，本发明涉及由（a）糖基化免疫球蛋白或其片段连接而形成的糖基化融合蛋白，其中具有形成一个或多个Asn-X-Ser / Thr序列另外被糖基化，（b）至少一种生物活性蛋白质或其部分，编码该基因的基因，包含该基因的重组表达载体，用重组表达载体转化或转染的宿主细胞，以及方法 制备糖基化融合蛋白，包括培养转化体或转染子并从培养物中分离糖基化的融合蛋白，以及包含如此制备的糖基化融合蛋白的药物组合物。

公开(公告)号：US08871907B2

公开(公告)日：2014-10-28

申请号：US10555538

申请日：2005-05-31

申请人： Yong-Hoon Chung ,  Ki-Wan Yi ,  Hoon-Sik Cho ,  Hong-Gyu Park ,  Kwang-Seong Kim

发明人： Yong-Hoon Chung ,  Ki-Wan Yi ,  Hoon-Sik Cho ,  Hong-Gyu Park ,  Kwang-Seong Kim

IPC分类号： C07K16/00 ,  C12P21/08 ,  A61K38/17 ,  C07K1/00

CPC分类号： A61K38/1774 ,  C07K2317/21 ,  C07K2317/41 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/94 ,  C07K2319/30

摘要： The present invention relates to a glycosylated immunoglobulin or a fragment thereof, in which an immunoglobulin variant, comprising one or more amino acid modifications selected from the group consisting of M160N, A195N, T243N, E265N, Y299T, F331T and Q346N, is additionally glycosylated, and a gene encoding the same. Also, the present invention relates to a glycosylated fusion protein formed as a result of linkage of (a) a glycosylated immunoglobulin or a fragment thereof, in which an immunoglobulin variant having a modified amino acid sequence forming one or more Asn-X-Ser/Thr sequences is additionally glycosylated, with (b) at least one biologically active protein or a portion thereof, a gene encoding the same, a recombination expression vector comprising the gene, a host cell transformed or transfected with the recombinant expression vector, and a method of preparing a glycosylated fusion protein comprising culturing the transformant or transfectant and isolating the glycosylated fusion protein from the culture, and a pharmaceutical composition comprising the glycosylated fusion protein thus prepared.

摘要翻译： 本发明涉及糖基化免疫球蛋白或其片段，其中包含一个或多个选自M160N，A195N，T​​243N，E265N，Y299T，F331T和Q346N的氨基酸修饰的免疫球蛋白变体另外被糖基化， 和编码该基因的基因。 此外，本发明涉及由（a）糖基化免疫球蛋白或其片段连接而形成的糖基化融合蛋白，其中具有形成一个或多个Asn-X-Ser / Thr序列另外被糖基化，（b）至少一种生物活性蛋白质或其部分，编码该基因的基因，包含该基因的重组表达载体，用重组表达载体转化或转染的宿主细胞，以及方法 制备糖基化融合蛋白，包括培养转化体或转染子并从培养物中分离糖基化的融合蛋白，以及包含如此制备的糖基化融合蛋白的药物组合物。

公开(公告)号：US20130089513A1

公开(公告)日：2013-04-11

申请号：US13437701

申请日：2012-04-02

申请人： Yong-Hoon Chung ,  Hak-sup Lee ,  Ki-Wan Yi ,  Youn-Hwa Heo ,  Jae-Youn Kim

发明人： Yong-Hoon Chung ,  Hak-sup Lee ,  Ki-Wan Yi ,  Youn-Hwa Heo ,  Jae-Youn Kim

IPC分类号： C07K14/52 ,  C07K14/535

CPC分类号： C07K14/52 ,  A61K38/00 ,  C07K14/524 ,  C07K14/535 ,  C07K14/715

摘要： Disclosed is a protein variant which substitutes valine for phenylalanine residue in a binding domain having a biological response-modifying function by binding to a receptor, ligand or substrate. Also, the present invention discloses a DNA encoding the protein variant, a recombinant expression vector to which the DNA is operably linked, a host cell transformed or transfected with the recombinant expression vector, and a method of preparing the protein variant comprising cultivating the host cell and isolating the protein variant from the resulting culture. Further, the present invention discloses a pharmaceutical composition comprising the protein variant and a pharmaceutically acceptable carrier.

摘要翻译： 公开了一种蛋白质变体，其通过结合受体，配体或底物，在具有生物反应调节功能的结合结构域中代替缬氨酸作为苯丙氨酸残基。 此外，本发明公开了编码蛋白质变体的DNA，可操作地连接DNA的重组表达载体，用重组表达载体转化或转染的宿主细胞，以及制备蛋白质变体的方法，包括培养宿主细胞 并从所得培养物中分离蛋白质变体。 此外，本发明公开了包含蛋白质变体和药学上可接受的载体的药物组合物。

公开(公告)号：US07569361B2

公开(公告)日：2009-08-04

申请号：US10519390

申请日：2004-05-27

申请人： Yong-Hoon Chung ,  Hak-sup Lee ,  Ki-Wan Yi ,  Youn-Hwa Heo ,  Jae-Youn Kim

发明人： Yong-Hoon Chung ,  Hak-sup Lee ,  Ki-Wan Yi ,  Youn-Hwa Heo ,  Jae-Youn Kim

IPC分类号： C07K14/00 ,  C07K14/475

CPC分类号： C07K14/52 ,  A61K38/00 ,  C07K14/524 ,  C07K14/535 ,  C07K14/715

摘要： Disclosed is a protein variant which substitutes valine for phenylalanine residue in a binding domain having a biological response-modifying function by binding to a receptor, ligand or substrate. Also, the present invention discloses a DNA encoding the protein variant, a recombinant expression vector to which the DNA is operably linked, a host cell transformed or transfected with the recombinant expression vector, and a method of preparing the protein variant comprising cultivating the host cell and isolating the protein variant from the resulting culture. Further, the present invention discloses a pharmaceutical composition comprising the protein variant and a pharmaceutically acceptable carrier.

摘要翻译： 公开了一种蛋白质变体，其通过结合受体，配体或底物，在具有生物反应调节功能的结合结构域中代替缬氨酸作为苯丙氨酸残基。 此外，本发明公开了编码蛋白质变体的DNA，可操作地连接DNA的重组表达载体，用重组表达载体转化或转染的宿主细胞，以及制备蛋白质变体的方法，包括培养宿主细胞 并从所得培养物中分离蛋白质变体。 此外，本发明公开了包含蛋白质变体和药学上可接受的载体的药物组合物。

公开(公告)号：US20100041586A1

公开(公告)日：2010-02-18

申请号：US12499710

申请日：2009-07-08

申请人： Yong-Hoon Chung ,  Hak-sup Lee ,  Ki-Wan Yi ,  Youn-Hwa Heo ,  Jae-Youn Kim

发明人： Yong-Hoon Chung ,  Hak-sup Lee ,  Ki-Wan Yi ,  Youn-Hwa Heo ,  Jae-Youn Kim

IPC分类号： A61K38/16

CPC分类号： C07K14/52 ,  A61K38/00 ,  C07K14/524 ,  C07K14/535 ,  C07K14/715

摘要： Disclosed is a protein variant which substitutes valine for phenylalanine residue in a binding domain having a biological response-modifying function by binding to a receptor, ligand or substrate. Also, the present invention discloses a DNA encoding the protein variant, a recombinant expression vector to which the DNA is operably linked, a host cell transformed or transfected with the recombinant expression vector, and a method of preparing the protein variant comprising cultivating the host cell and isolating the protein variant from the resulting culture. Further, the present invention discloses a pharmaceutical composition comprising the protein variant and a pharmaceutically acceptable carrier.

摘要翻译： 公开了一种蛋白质变体，其通过结合受体，配体或底物，在具有生物反应调节功能的结合结构域中代替缬氨酸作为苯丙氨酸残基。 此外，本发明公开了编码蛋白质变体的DNA，可操作地连接DNA的重组表达载体，用重组表达载体转化或转染的宿主细胞，以及制备蛋白质变体的方法，包括培养宿主细胞 并从所得培养物中分离蛋白质变体。 此外，本发明公开了包含蛋白质变体和药学上可接受的载体的药物组合物。

公开(公告)号：US20060008872A1

公开(公告)日：2006-01-12

申请号：US10519390

申请日：2004-09-30

申请人： Yong-Hoon Chung ,  Hak-sup Lee ,  Ki-Wan Yi ,  Youn-Hwa Heo ,  Jae-Youn Kim

发明人： Yong-Hoon Chung ,  Hak-sup Lee ,  Ki-Wan Yi ,  Youn-Hwa Heo ,  Jae-Youn Kim

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/00 ,  C12N15/85

CPC分类号： C07K14/52 ,  A61K38/00 ,  C07K14/524 ,  C07K14/535 ,  C07K14/715

摘要： Disclosed is a protein variant which substitutes valine for phenylalanine residue in a binding domain having a biological response-modifying function by binding to a receptor, ligand or substrate. Also, the present invention discloses a DNA encoding the protein variant, a recombinant expression vector to which the DNA is operably linked, a host cell transformed or transfected with the recombinant expression vector, and a method of preparing the protein variant comprising cultivating the host cell and isolating the protein variant from the resulting culture. Further, the present invention discloses a pharmaceutical composition comprising the protein variant and a pharmaceutically acceptable carrier.

摘要翻译： 公开了一种蛋白质变体，其通过结合受体，配体或底物，在具有生物反应调节功能的结合结构域中代替缬氨酸作为苯丙氨酸残基。 此外，本发明公开了编码蛋白质变体的DNA，可操作地连接DNA的重组表达载体，用重组表达载体转化或转染的宿主细胞，以及制备蛋白质变体的方法，包括培养宿主细胞 并从所得培养物中分离蛋白质变体。 此外，本发明公开了包含蛋白质变体和药学上可接受的载体的药物组合物。

公开(公告)号：US07229962B2

公开(公告)日：2007-06-12

申请号：US10363427

申请日：2002-07-26

申请人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

发明人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

IPC分类号： C07K14/715 ,  A61K38/16

CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment of an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端与生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端连接。 此外，本发明公开了通过在两个单体蛋白的铰链区上形成分子间二硫键而形成的二聚体蛋白，该两个单体蛋白是通过将免疫应答涉及免疫应答的两个相同的可溶胞外区的连接体与免疫球蛋白分子的Fc片段 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。

公开(公告)号：US20070110746A1

公开(公告)日：2007-05-17

申请号：US10539946

申请日：2005-02-18

申请人： Yong-Hoon Chung

发明人： Yong-Hoon Chung

IPC分类号： A61K39/395 ,  C07K16/28

CPC分类号： C07K14/715 ,  A61K38/00 ,  C07K14/70503 ,  C07K2319/30

摘要： Disclosed is a pharmaceutical composition for treating immunological disorders by inhibiting the activation of T lymphocytes, comprising, as active ingredients, two or more selected from the group consisting of a substance capable of blocking binding of an MHC (Major Histocompatibility Complex) Class II molecule and a receptor thereof, a substance capable of blocking binding of a costimulatory molecule and a receptor thereof, a substance capable of blocking binding of an adhesion molecule and a receptor thereof, and a substance capable of blocking binding of a cytokine and a receptor thereof.

摘要翻译： 公开了通过抑制T淋巴细胞的活化来治疗免疫学障碍的药物组合物，其包含作为活性成分的两种或更多种选自以下的物质：能够阻断MHC（主要组织相容性复合物）II类分子和 其受体，能够阻断共刺激分子及其受体结合的物质，能够阻断粘附分子及其受体结合的物质，以及能够阻断细胞因子及其受体结合的物质。

公开(公告)号：US08372961B2

公开(公告)日：2013-02-12

申请号：US12692392

申请日：2010-01-22

申请人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

发明人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

IPC分类号： C12N15/12

CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment of an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端与生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端连接。 此外，本发明公开了通过在两个单体蛋白的铰链区上形成分子间二硫键而形成的二聚体蛋白，该两个单体蛋白是通过将免疫应答涉及免疫应答的两个相同的可溶胞外区的连接体与免疫球蛋白分子的Fc片段 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。

公开(公告)号：US07670602B2

公开(公告)日：2010-03-02

申请号：US11739288

申请日：2007-04-24

申请人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

发明人： Yong-Hoon Chung ,  Ji-Woong Han ,  Hye-Ja Lee ,  Eun-Yong Choi ,  Jin-Mi Kim

IPC分类号： A61K39/395

CPC分类号： C07K14/70507 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7151 ,  C07K2319/00 ,  C07K2319/30

摘要： Disclosed are concatameric proteins comprising two soluble domains, in which the C-terminus of a soluble domain of a biologically active protein is linked to the N-terminus of an identical soluble domain or a distinct soluble domain of a biologically active protein. Also, the present invention discloses dimeric proteins formed by formation of intermolecular disulfide bonds at the hinge region of two monomeric proteins formed by linkage of a concatamer of two identical soluble extracellular regions of proteins involving immune response to an Fc fragment of an immunoglobulin molecule, their glycosylated proteins, DNA constructs encoding the monomeric proteins, recombinant expression plasmids containing the DNA construct, host cells transformed or transfected with the recombinant expression plasmids, and a method of preparing the dimeric proteins by culturing the host cells. Further, the present invention discloses pharmaceutical or diagnostic compositions comprising the dimeric protein or its glycosylated form.

摘要翻译： 公开了包含两个可溶性结构域的连续蛋白质，其中生物活性蛋白质的可溶性结构域的C末端与生物活性蛋白质的相同可溶性结构域或不同可溶性结构域的N末端连接。 此外，本发明公开了通过在两个单体蛋白的铰链区上形成分子间二硫键而形成的二聚体蛋白，该两个单体蛋白是通过将免疫应答涉及免疫应答的两个相同的可溶胞外区的连接体与免疫球蛋白分子的Fc片段 糖基化蛋白质，编码单体蛋白的DNA构建体，含有DNA构建体的重组表达质粒，用重组表达质粒转化或转染的宿主细胞，以及通过培养宿主细胞制备二聚体蛋白质的方法。 此外，本发明公开了包含二聚体蛋白质或其糖基化形式的药物或诊断组合物。