In vivo electroporation method for early stage embryo of chickens
    6.
    发明授权
    In vivo electroporation method for early stage embryo of chickens 失效
    鸡早期胚胎的体内电穿孔法

    公开(公告)号:US06563017B2

    公开(公告)日:2003-05-13

    申请号:US09214565

    申请日:1999-09-13

    IPC分类号: C12N1587

    摘要: A gene is transferred to the early stage chicken embryo by electroporation with placing the electrode tips to hold both ends of the embryo. Transfer of the desired gene to the early stage embryo was detected using the &bgr;-galactosidase gene of E coli as a reporter gene. The expression of the gene in the early stage embryo of chicken was thus confirmed. This method for transferring a gene into undifferentiated cells is simple to implement, highly efficient in transferring a gene, and applicable to various animal species.

    摘要翻译: 通过电穿孔将基因转移到早期鸡胚中,放置电极末端以保持胚胎的两端。 使用大肠杆菌的β-半乳糖苷酶基因作为报告基因检测到所需基因转移到早期胚胎。 因此证实了鸡早期胚胎中基因的表达。 将基因转移到未分化细胞中的方法简单实现,转移基因高效,适用于各种动物物种。