-
1.发明授权公开(公告)号：US06723532B2
公开(公告)日：2004-04-20
申请号：US09070938
申请日：1998-04-30
申请人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Makoto Asakawa , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
发明人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Makoto Asakawa , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
IPC分类号： C12N1500
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2740/16122 , C12N2760/18821 , C12N2760/18822 , C12N2760/18843 , C12N2760/18852
摘要： A method for reconstituting Sendai viral particles by transfecting Sendai virus to a host expressing all genes for the initial replication has been developed, enabling the Production of negative strand RNA vectors highly useful for practical applications.
摘要翻译： 已经开发了通过将仙台病毒转染到表达用于初始复制的所有基因的宿主来重建仙台病毒颗粒的方法，使得能够生产对实际应用非常有用的负链RNA载体。
-
2.发明授权公开(公告)号：US06645760B2
公开(公告)日：2003-11-11
申请号：US09471840
申请日：1999-12-23
申请人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Makoto Asakawa , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
发明人： Yoshiyuki Nagai , Atsushi Kato , Fukashi Murai , Makoto Asakawa , Tsuneaki Sakata , Mamoru Hasegawa , Tatsuo Shioda
IPC分类号： C12N500
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2740/16122 , C12N2760/18821 , C12N2760/18822 , C12N2760/18843 , C12N2760/18852
摘要： A method for reconstituting Sendai viral particles by transfecting Sendai virus to a host expressing all genes for the initial replication has been developed, enabling the production of negative strand RNA vectors highly useful for practical applications.
摘要翻译： 已经开发了通过将仙台病毒转染到表达用于初始复制的所有基因的宿主来重建仙台病毒颗粒的方法，使得能够生产对实际应用非常有用的负链RNA载体。
-
公开(公告)号：US20050158279A1
公开(公告)日：2005-07-21
申请号：US10840800
申请日：2004-05-06
CPC分类号： C12Y302/01031 , A61K48/00 , C07K14/50 , C12N9/2402 , C12N15/86 , C12N2760/18843 , C12N2760/18871
摘要： A (−)-strand RNA virus vector for transferring a gene into nerve cells which makes it possible to efficiently transfer a gene into nerve cells including the central nervous system tissues in gene therapy, etc.
摘要翻译： 用于将基因转移到神经细胞中的（ - ） - 链RNA病毒载体，其使得可以有效地将基因转移到包括基因治疗中的中枢神经系统组织的神经细胞等中。
-
公开(公告)号：US20050271628A1
公开(公告)日：2005-12-08
申请号：US11178978
申请日：2005-07-12
CPC分类号： C07K14/50 , A61K38/00 , A61K38/185 , A61K48/00 , A61K48/0075 , C12N9/2402 , C12N15/86 , C12N2760/18832 , C12N2760/18843 , C12Y302/01031
摘要： Use of a negative-sense RNA virus vector has enabled transfer of nucleic acid into nerve cells. The method of this invention can be used for introducing a gene efficiently into nerve cells including the central nerve tissue in gene therapy, etc.
摘要翻译： 使用负义RNA病毒载体使核酸能够转移到神经细胞中。 本发明的方法可用于将基因有效地引入包括基因治疗中的中枢神经组织等神经细胞等。
-
5.发明授权Sendai viral vectors comprising foreign genes inserted between the R1 and R2 Loci 失效包含插入在R1和R2基因座之间的外来基因的仙台病毒载体公开(公告)号：US07241617B2
公开(公告)日：2007-07-10
申请号：US09843922
申请日：2001-04-30
CPC分类号： C07K14/50 , A61K38/00 , A61K38/185 , A61K48/00 , A61K48/0075 , C12N9/2402 , C12N15/86 , C12N2760/18832 , C12N2760/18843 , C12Y302/01031
摘要： Use of a negative-sense RNA virus vector has enabled transfer of nucleic acid into nerve cells. The method of this invention can be used for introducing a gene efficiently into nerve cells including the central nerve tissue in gene therapy, etc.
摘要翻译： 使用负义RNA病毒载体使核酸能够转移到神经细胞中。 本发明的方法可用于将基因有效地引入包括基因治疗中的中枢神经组织等神经细胞等。
-
公开(公告)号：US07226786B2
公开(公告)日：2007-06-05
申请号：US10316535
申请日：2002-12-10
申请人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Fumino Tokito , Takahiro Hirata , Tsuyoshi Tokusumi , Makoto Inoue , Yumiko Tokusumi
发明人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Fumino Tokito , Takahiro Hirata , Tsuyoshi Tokusumi , Makoto Inoue , Yumiko Tokusumi
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , C12N7/00 , C12N2760/18823 , C12N2760/18843 , C12N2760/18845 , C12N2760/18861 , C12N2800/30 , C12N2810/6081
摘要： F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.
摘要翻译： 通过使用F基因缺陷的仙台病毒基因组cDNA成功地回收了F基因缺陷型病毒粒子。 此外，通过使用F表达细胞作为辅助细胞，成功构建了F基因缺陷型感染性病毒颗粒。 此外，通过使用F基因和HN基因缺陷的病毒基因组cDNA，成功地回收了F基因和HN基因缺陷型病毒粒子。 此外，通过使用F-和HN表达细胞作为辅助细胞，F基因和HN基因缺陷型感染性病毒颗粒成功产生。 通过使用F表达细胞作为辅助细胞构建F基因和HN基因并具有F蛋白的病毒。 此外，使用表达M蛋白的辅助细胞产生M基因缺陷型感染性病毒颗粒。 从感染M基因缺陷病毒的细胞中，病毒样颗粒的释放被抑制。 此外，通过使用表达VSV-G的细胞成功地构建了VSV-G伪型病毒。 用于构建这些缺陷病毒的技术有助于可用于基因治疗的副粘病毒科的载体的发育。
-
公开(公告)号：US20070009949A1
公开(公告)日：2007-01-11
申请号：US11515341
申请日：2006-09-01
申请人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Takahiro Hirata , Makoto Inoue , Yumiko Tokusumi
发明人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Takahiro Hirata , Makoto Inoue , Yumiko Tokusumi
CPC分类号： C07K14/005 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , C12N7/00 , C12N15/86 , C12N2710/24143 , C12N2760/18811 , C12N2760/18822 , C12N2760/18823 , C12N2760/18843 , C12N2760/18845 , C12N2760/18861 , C12N2760/20222 , C12N2800/30 , C12N2810/6081
摘要： A functional RNP containing negative-strand single-stranded RNA derived from Sendai virus, which has been modified so as not to express at least one envelope protein, has been successfully prepared. An RNP comprising a foreign gene is prepared and inserted into a cell with the use of a cationic liposome, thereby successfully expressing the foreign gene.
摘要翻译： 已经成功地制备了已经被修饰以便不表达至少一种包膜蛋白的来源于仙台病毒的负链单链RNA的功能性RNP。 制备包含外来基因的RNP，并使用阳离子脂质体将其插入细胞中，从而成功地表达外源基因。
-
公开(公告)号：US4087729A
公开(公告)日：1978-05-02
申请号：US678673
申请日：1976-04-20
申请人： Shin-ichi Yamazaki , Makoto Asakawa , Tadao Saito
发明人： Shin-ichi Yamazaki , Makoto Asakawa , Tadao Saito
IPC分类号： H01J37/20 , B23Q1/36 , B23Q1/62 , G03F7/20 , G03F9/00 , G05B19/39 , G05B19/402 , G05D3/00 , H01J37/304 , H01L21/027 , H01L21/68 , H02K41/00 , G05B19/28
CPC分类号： G03F7/70716 , B23Q1/36 , B23Q1/623 , G05B19/39 , G05B19/402 , H01J37/3045 , G05B2219/34242 , G05B2219/37272 , G05B2219/41105 , G05B2219/41344 , G05B2219/41345 , G05B2219/41457 , G05B2219/45026 , G05B2219/50046 , G05B2219/50151
摘要： The position adjusting apparatus comprises a stationary frame, first and second movable stages respectively, disposed on X and Y axes intersecting at right angles, a plurality of dynamic type electro-mechanical transducers mounted on the axial ends of each movable stage, a first resilient supporting member for mounting the first movable stage to be movable in the direction of X axis, a second resilient supporting member mounted on the first movable stage forming the second movable stage in the direction of the Y axis, and a platform mounted on the second movable stage for supporting an object whose position is to be adjusted.
摘要翻译： 位置调整装置包括：固定框架，分别设置在与直角相交的X和Y轴上的第一和第二可移动台，安装在每个可移动台的轴向端部上的多个动态型机电换能器，第一弹性支撑 用于将第一可移动台安装成沿X轴方向移动的构件，安装在第一可移动台上沿Y轴方向形成第二可动台的第二弹性支撑构件，以及安装在第二活动台上的平台 用于支持要调整其位置的对象。
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.034 秒)
