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17 条记录 (耗时 0.034 秒)

    METHOD AND APPARATUS FOR CONCENTRATING AND AMPLIFYING NUCLEIC ACID IN SINGLE MICRO CHAMBER
    3.
    发明申请
    METHOD AND APPARATUS FOR CONCENTRATING AND AMPLIFYING NUCLEIC ACID IN SINGLE MICRO CHAMBER 有权
    在单个微室中浓缩和放大核酸的方法和装置

    公开(公告)号：US20070269819A1

    公开(公告)日：2007-11-22

    申请号：US11620961

    申请日：2007-01-08

    申请人： Young-rok KIM ,  Jun-hong MIN ,  In-ho LEE ,  Young-sun LEE ,  Chang-eun YOO ,  Ki-woong HAN

    发明人： Young-rok KIM ,  Jun-hong MIN ,  In-ho LEE ,  Young-sun LEE ,  Chang-eun YOO ,  Ki-woong HAN

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： C12Q1/6806 ,  B01L3/5027 ,  B01L3/5088 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2527/125

    摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.

    摘要翻译： 在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可变性盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸 酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。 由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。 此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低 通过消除样品的运输而损失。 因此容易提供用于浓缩和扩增核酸的完整的自动化系统。

    METHOD AND APPARATUS FOR PURIFYING NUCLEIC ACID ON HYDROPHILIC SURFACE OF SOLID SUPPORT USING HYDROGEN BONDING
    4.
    发明申请
    METHOD AND APPARATUS FOR PURIFYING NUCLEIC ACID ON HYDROPHILIC SURFACE OF SOLID SUPPORT USING HYDROGEN BONDING 审中-公开
    使用氢键连接固体支持的亲水表面上的核酸的方法和装置

    公开(公告)号：US20070238109A1

    公开(公告)日：2007-10-11

    申请号：US11553657

    申请日：2006-10-27

    申请人： Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

    发明人： Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： C12N15/1006 ,  C12N15/1003

    摘要： Provided is a method of purifying nucleic acid, the method including: contacting a nucleic acid-containing sample and a solution containing a kosmotropic salt on a solid support having a hydrophilic functional group on its surface to bind the nucleic acid to the solid support. Since the solid support is used as it is without any surface treatment, manufacture of the apparatus is very easy, and nucleic acid can be bound to the solid support without specific additives in a wide pH range, so that the apparatus can be used for a Lab-On-a-Chip.

    摘要翻译： 提供了一种纯化核酸的方法，所述方法包括：使含核酸的样品和含有可变色盐的溶液在其表面上具有亲水性官能团的固体支持物上接触，以将核酸结合到固体支持物上。 由于固体支持物原样使用而没有进行任何表面处理，所以装置的制造非常容易，并且核酸可以在宽的pH范围内结合固体支持物而没有特定的添加剂，使得该装置可以用于 实验室芯片

    APPARATUS FOR INTRODUCING FLUID INTO MICROFLUIDIC CHIP BY USING CENTRIFUGAL FORCE, A SYSTEM INCLUDING THE APPARATUS, AND A METHOD OF USING THE APPARATUS
    5.
    发明申请
    APPARATUS FOR INTRODUCING FLUID INTO MICROFLUIDIC CHIP BY USING CENTRIFUGAL FORCE, A SYSTEM INCLUDING THE APPARATUS, AND A METHOD OF USING THE APPARATUS 有权
    通过使用离心力将流体引入微流化芯片的装置，包括装置的系统以及使用装置的方法

    公开(公告)号：US20080072994A1

    公开(公告)日：2008-03-27

    申请号：US11846794

    申请日：2007-08-29

    申请人： Young-rok KIM ,  Jun-hong MIN ,  Kak NAMKOONG ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Ki-woong HAN ,  Ki-eun KIM

    发明人： Young-rok KIM ,  Jun-hong MIN ,  Kak NAMKOONG ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Ki-woong HAN ,  Ki-eun KIM

    IPC分类号： B65B1/14

    CPC分类号： B01L9/527 ,  B01L3/5021 ,  B01L3/5027 ,  B01L2200/027 ,  B01L2300/0832 ,  B01L2400/0409 ,  C12M23/16

    摘要： An apparatus introducing a fluid using a centrifugal force includes an introduction member including a chip receiver and a fluid introduction reservoir, the chip receiver receiving a first part of a microfluidic chip, the first part including an inlet, the fluid introduction reservoir storing a fluid to be introduced to the microfluidic chip, the fluid introduction reservoir having an exit formed to correspond to the inlet of the microfluidic chip received in the chip receiver, and a support member supporting a second part of the microfluidic chip, wherein the microfluidic chip is disposed between the introduction member and the support member, the apparatus is rotatable in a state where the introduction member is closer to a center of rotation than the microfluidic chip, and the fluid is introducible from the fluid introduction reservoir through the inlet into the microfluidic chip due to a centrifugal force generated by rotation.

    摘要翻译： 使用离心力引入流体的装置包括引入构件，其包括芯片接收器和流体引入储存器，所述芯片接收器接收微流体芯片的第一部分，所述第一部分包括入口，所述流体引入储存器将流体存储到 引入到微流体芯片中，流体引入储存器具有形成为对应于接收在芯片接收器中的微流体芯片的入口的出口，以及支撑微流体芯片的第二部分的支撑构件，其中微流体芯片设置在 引导构件和支撑构件，该装置可以在导入构件比微流体芯片更靠近旋转中心的状态下旋转，并且流体可以从流体引入储存器通过入口引入到微流体芯片中，这是由于 由旋转产生的离心力。

    METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER
    7.
    发明申请
    METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER 审中-公开
    使用微珠和激光快速破坏细胞或病毒的方法和装置

    公开(公告)号：US20080199930A1

    公开(公告)日：2008-08-21

    申请号：US12031947

    申请日：2008-02-15

    申请人： Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM ,  Myo-yong LEE ,  Shin-i YOO ,  Yeon-ja CHO ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Seung-yeon YANG

    发明人： Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM ,  Myo-yong LEE ,  Shin-i YOO ,  Yeon-ja CHO ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Seung-yeon YANG

    IPC分类号： C12N13/00 ,  C12M1/00

    CPC分类号： C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

    摘要： A method and apparatus for rapid disruption of cells or viruses using beads and a laser are provided. According to the method and apparatus for rapid disruption of cells or viruses using beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

    摘要翻译： 提供了使用珠和激光来快速破坏细胞或病毒的方法和装置。 根据使用珠粒和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，可以使用激光二极管使装置小型化，DNA纯化步骤可以在细胞破碎后直接进行 或病毒，并且含有DNA的溶液可以转移到随后的步骤中，在用电磁体除去后续反应的抑制剂附着的细胞碎片和珠粒之后。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决 裂解芯片可应用于LOC。