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    DRIED COMPOSITION FOR HOT-START PCR WITH LONG-TERM STABILITY
    2.
    发明申请
    DRIED COMPOSITION FOR HOT-START PCR WITH LONG-TERM STABILITY 有权
    用于具有长期稳定性的热启动PCR的干燥组合物

    公开(公告)号：US20100209973A1

    公开(公告)日：2010-08-19

    申请号：US12682456

    申请日：2008-10-28

    申请人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C12P19/34 ,  C12N9/14

    CPC分类号： C12Q1/686 ,  C12Q2549/101 ,  C12Q2527/125

    摘要： The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long-term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.

    摘要翻译： 本发明涉及一种用于热启动PCR的干燥组合物，更准确地说是一种用于热启动PCR的干燥组合物，其具有改进的稳定性和长期储存性，其特征在于通过以下步骤制备反应混合物：将含有 反应缓冲液，MgCl 2，4种dNTP，反应管中含有焦磷酸的DNA聚合酶和焦磷酸酶; 并干燥上述制备的反应混合物，其制备方法和使用其制备用于扩增核酸的方法。 用于热启动PCR的干燥组合物在干燥之前加入焦磷酸盐和焦磷酸酶，与常规的热启动组合物相比，其可以具有改善的稳定性和长期储存性以及使用方便性。 因此，该组合物可有效用于热启动PCR，多重PCR或实时定量PCR。

    Dried composition for hot-start PCR with long-term stability
    3.
    发明授权
    Dried composition for hot-start PCR with long-term stability 有权
    用于热启动PCR的干组合物具有长期稳定性

    公开(公告)号：US09034603B2

    公开(公告)日：2015-05-19

    申请号：US12682456

    申请日：2008-10-28

    申请人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Seong-Youl Kim ,  Hyun Bae Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q2549/101 ,  C12Q2527/125

    摘要： The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long-term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.

    摘要翻译： 本发明涉及一种用于热启动PCR的干燥组合物，更准确地说是一种用于热启动PCR的干燥组合物，其具有改进的稳定性和长期储存性，其特征在于通过以下步骤制备反应混合物：将含有 反应缓冲液，MgCl 2，4种dNTP，反应管中含有焦磷酸的DNA聚合酶和焦磷酸酶; 并干燥上述制备的反应混合物，其制备方法和使用其制备用于扩增核酸的方法。 用于热启动PCR的干燥组合物在干燥之前加入焦磷酸盐和焦磷酸酶，与常规的热启动组合物相比，其可以具有改善的稳定性和长期储存性以及使用方便性。 因此，该组合物可有效用于热启动PCR，多重PCR或实时定量PCR。

    Method for isolating a nucleic acid using particulate matter and a composition therefor
    4.
    发明授权
    Method for isolating a nucleic acid using particulate matter and a composition therefor 有权
    使用颗粒物分离核酸的方法及其组合物

    公开(公告)号：US08530639B2

    公开(公告)日：2013-09-10

    申请号：US12448134

    申请日：2007-12-11

    申请人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C07H21/00

    CPC分类号： C07H21/00 ,  C12N15/1003

    摘要： A method for isolating a nucleic acid from a biological sample includes applying particulate matter to promote co-aggregation and co-precipitation of insoluble aggregate by directly adding to the biological sample, adding to the biological sample in admixture with a cell lysis buffer, adding to the biological sample treated with a cell lysis buffer, adding to cell lysates in admixture with a buffer for forming denatured protein aggregate; or adding to cell lysates comprising the formed denatured protein aggregate. The particulate matter is selected from the group consisting of a material formed from an element of Ag, Fe, Ti, Al, Sn, Si, Cu, Mo, Ni, W or Zn, an oxide, a carbide, a nitride, a boride and a silicide thereof, and a mixture thereof, a polymer selected from PMMA (Poly Methyl MethAcrylate), polyethylene or polyurethane; and a mixture thereof. The insoluble aggregate comprises denatured protein aggregate and cell debris.

    摘要翻译： 从生物样品中分离核酸的方法包括通过直接添加到生物样品中来加入颗粒物质以促进不溶性聚集体的共聚和共沉淀，将生物样品与细胞裂解缓冲液混合，加入到 用细胞裂解缓冲液处理生物样品，加入细胞裂解物与缓冲液混合以形成变性蛋白质聚集体; 或加入包含形成的变性蛋白质聚集体的细胞裂解物。 颗粒物质选自由Ag，Fe，Ti，Al，Sn，Si，Cu，Mo，Ni，W或Zn的元素形成的材料，氧化物，碳化物，氮化物，硼化物 及其硅化物及其混合物，选自PMMA（聚甲基丙烯酸甲酯），聚乙烯或聚氨酯的聚合物; 及其混合物。 不溶性聚集体包含变性蛋白质聚集体和细胞碎片。

    METHOD FOR ISOLATING A NUCLEIC ACID USING PARTICULATE MATTER AND A COMPOSITION THEREFOR
    5.
    发明申请
    METHOD FOR ISOLATING A NUCLEIC ACID USING PARTICULATE MATTER AND A COMPOSITION THEREFOR 有权
    使用颗粒物分解核酸的方法及其组合物

    公开(公告)号：US20100197903A1

    公开(公告)日：2010-08-05

    申请号：US12448134

    申请日：2007-12-11

    申请人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C07H21/00 ,  B01J20/26 ,  C09K3/00

    CPC分类号： C07H21/00 ,  C12N15/1003

    摘要： Disclosed are a method for isolating a nucleic acid using particulate matter and a composition therefor. The method comprises essentially the steps of adding a lysis buffer and a neutralization buffer to a biological sample sequentially, and centrifuging cell lysates for at least 10 minutes to separate a solution comprising the nucleic acid and insoluble aggregate comprising denatured protein aggregate and cell debris. The particulate matter is added to and participates in co-aggregation and co-precipitation of denatured protein aggregate and cell debris, and co-aggregates and co-precipitates denatured protein aggregate and cell debris within a much shortened time, thereby to shorten the time and to increase the yield for the isolation of a nucleic acid, compared with conventional methods.

    摘要翻译： 公开了使用颗粒物质及其组合物分离核酸的方法。 该方法基本上包括以下步骤：依次向生物样品中加入裂解缓冲液和中和缓冲液，并离心细胞裂解物至少10分钟以分离包含核酸的溶液和包含变性蛋白质聚集体和细胞碎片的不溶性骨架。 添加颗粒物并参与变性蛋白质聚集体和细胞碎片的共聚和共沉淀，并在更短的时间内共同聚集和共沉淀变性蛋白质聚集体和细胞碎片，从而缩短时间和 以提高分离核酸的产量，与常规方法相比。

    Cyclic reverse transcription method
    6.
    发明授权
    Cyclic reverse transcription method 有权
    循环逆转录法

    公开(公告)号：US08058007B2

    公开(公告)日：2011-11-15

    申请号：US12531778

    申请日：2008-03-19

    申请人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6846 ,  C12Q2537/155 ,  C12Q2527/101 ,  C12Q2521/107

    摘要： Disclosed is a cyclic reverse transcription method, which comprises performing reverse transcription reaction in one or more cycles, each cycle comprising the steps of: (i) performing reaction with a reverse transcription reaction solution comprising template RNA, RNA-dependent DNA polymerase, a reaction buffer, primers for reverse transcription and dNTPs, and optionally, a stabilizer, at 10° C. to 40° C. and, (ii) performing reaction with the resultant reaction solution at 42° to 55° C.

    摘要翻译： 公开了一种循环逆转录方法，其包括在一个或多个循环中进行逆转录反应，每个循环包括以下步骤：（i）与包含模板RNA，RNA依赖性DNA聚合酶的反转录反应溶液，反应 缓冲液，用于逆转录的引物和dNTP，以及任选的稳定剂，在10℃至40℃下，和（ii）在42℃至55℃下与所得反应溶液进行反应。

    CYCLIC REVERSE TRANSCRIPTION METHOD
    7.
    发明申请
    CYCLIC REVERSE TRANSCRIPTION METHOD 有权
    循环逆转录方法

    公开(公告)号：US20100221786A1

    公开(公告)日：2010-09-02

    申请号：US12531778

    申请日：2008-03-19

    申请人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Hui Jeong Jeong ,  Min-Jung Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6846 ,  C12Q2537/155 ,  C12Q2527/101 ,  C12Q2521/107

    摘要： Disclosed is a cyclic reverse transcription method, which comprises performing reverse transcription reaction in one or more cycles, each cycle comprising the steps of: (i) performing reaction with a reverse transcription reaction solution comprising template RNA, RNA-dependent DNA polymerase, a reaction buffer, primers for reverse transcription and dNTPs, and optionally, a stabilizer, at 10° C. to 40° G and, (ii) performing reaction with the resultant reaction solution at 42° to 55° C.

    摘要翻译： 公开了一种循环逆转录方法，其包括在一个或多个循环中进行逆转录反应，每个循环包括以下步骤：（i）与包含模板RNA，RNA依赖性DNA聚合酶的反转录反应溶液，反应 缓冲液，用于逆转录的引物和dNTP，以及任选的稳定剂，在10℃至40℃下，和（ii）在42℃至55℃下与所得反应溶液进行反应。

    DNA and peptides of a diabetes-specific endogenous retrovirus
    8.
    发明授权
    DNA and peptides of a diabetes-specific endogenous retrovirus 失效
    糖尿病特异性内源性逆转录病毒的DNA和肽

    公开(公告)号：US06365727B1

    公开(公告)日：2002-04-02

    申请号：US09120653

    申请日：1998-07-22

    申请人： Ji-Won Yoon ,  Hee-Sook Jun ,  Hae-Joon Park ,  Jong Seong Ahn ,  Young-Ju Ha ,  Soo-Il Chung

    发明人： Ji-Won Yoon ,  Hee-Sook Jun ,  Hae-Joon Park ,  Jong Seong Ahn ,  Young-Ju Ha ,  Soo-Il Chung

    IPC分类号： C07H2104

    CPC分类号： C12Q1/702

    摘要： The present invention relates to gene and peptide sequences of a diabetes-specific endogenous retrovirus which is derived from type 1 diabetes patients. In particular, the present invention relates to a whole genome of the diabetes-specific variant of endogenous retrovirus (ERV-9) purified from pancreatic tissues of type 1 diabetes (insulin-dependent diabetes mellitus [IDDM]) patients and its genes and peptide and their sequences, which can be used as a diagnosing reagent for type 1 diabetes and as an immunogen. The diabetes-specific retrovirus expressed exclusively in pancreatic beta cells was purified from deceased type 1 diabetes patients. Subsequently, the retroviral gene sequences were determined, and by analyzing the amino acid sequence of the peptide deduced from the gene, 21 domains of the peptide having hydrophilicity and immuno-dominancy were identified. Therefore, the variant gene of the endogenous retrovirus and the peptide deduced from the gene can be effectively used as a diagnosing reagent of autoimmune-antibody for type 1 diabetes and as a vaccine for the variant ERV-9 related diseases.

    摘要翻译： 本发明涉及衍生自1型糖尿病患者的糖尿病特异性内源性逆转录病毒的基因和肽序列。 特别地，本发明涉及从1型糖尿病（胰岛素依赖性糖尿病[IDDM]）患者的胰组织纯化的内源性逆转录病毒（ERV-9）的糖尿病特异性变体及其基因和肽的全基因组， 它们的序列可用作1型糖尿病的诊断试剂和免疫原。仅在死亡的1型糖尿病患者中纯化了在胰腺β细胞中表达的糖尿病特异性逆转录病毒。 随后，测定逆转录病毒基因序列，通过分析从该基因推定的肽的氨基酸序列，鉴定出具有亲水性和免疫多肽的21个结构域。因此，内源性逆转录病毒和肽的变体基因 从该基因推测可以有效地用作1型糖尿病的自身免疫抗体的诊断试剂和用于变异型ERV-9相关疾病的疫苗。