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    DNA probe diffraction assay and reagents
    1.
    发明授权
    DNA probe diffraction assay and reagents 失效
    DNA探针衍生物测定和试剂

    公开(公告)号：US5089387A

    公开(公告)日：1992-02-18

    申请号：US216691

    申请日：1988-07-07

    申请人： Yuh-Geng Tsay ,  Emanuel Calenoff ,  Eric K. Gustafson ,  Rick Trebino ,  John Lee

    发明人： Yuh-Geng Tsay ,  Emanuel Calenoff ,  Eric K. Gustafson ,  Rick Trebino ,  John Lee

    IPC分类号： G01N21/47 ,  C12Q1/68 ,  G01N21/77 ,  G01N33/543 ,  G02B5/18

    CPC分类号： G01N21/4788 ,  C12Q1/6825 ,  G01N33/54373 ,  G01N2021/757

    摘要： The assay of the subject invention uses DNA sequences as probes in a nucleic acid hybridization diffraction assay, to detect specific DNA sequences in a sample. Diffraction assay methodologies are applied to determine the presence and amount of analyte.This invention involves a discovery in the areas of supporting surfaces for a biogrid or biograting which provide greatly reduced non-specific hybridization and binding. A preferred process of this invention involves manufacturing a biograting for use in a light diffraction assay, and comprises adhering a uniform layer of hybridizing reagent comprising a nucleotide sequence on a smooth, solid surface and exposing the surface to UV radiation through a shadow mask with a diffraction grating pattern of lines to selectively deactivate the hybridizing reagent, leaving a biological diffraction grating design of lines of active hybridizing reagent. The smooth, solid surface is preferably selected from the group consisting of polysilicon and single crystalline silicon surfaces.The diffraction hybridizing assay method of this invention for determining the presence or quantity of an analyte in an aqueous sample comprises contacting a nucleic acid sequence diffraction biogrid with the sample under proper circumstances and for a sufficient time to permit nucleic acid hybridization between a nucleic acid sequence probe and an analyte; separating the biogrid from the sample; illuminating the biogrid with light from a light source; and determining the light diffracted by the diffraction hybridization assay surface.

    Highly reflective biogratings
    2.
    发明授权
    Highly reflective biogratings 失效
    高度反光的生态化

    公开(公告)号：US5478527A

    公开(公告)日：1995-12-26

    申请号：US342486

    申请日：1994-11-21

    申请人： Eric K. Gustafson ,  John Lee ,  Yuh-Geng Tsay

    发明人： Eric K. Gustafson ,  John Lee ,  Yuh-Geng Tsay

    IPC分类号： G01N21/47 ,  G01N33/543 ,  G01N33/553 ,  G01N33/545

    CPC分类号： G01N33/54373 ,  G01N21/4788 ,  G01N2021/7773 ,  Y10S436/805

    摘要： A reflective biograting consists of an optically flat layer of a transparent composition such as silicon dioxide having a first and second surface, alternating zones of active and inactive binding reagent on the first surface, and a reflective metal layer having a thickness of at least above 1000 .ANG.. The reflective metal layer can be supported on an optically flat surface of a wafer, and the reflective metal can be aluminum, silver, gold, chromium, nickel, titanium or platinum coating on a polished wafer. Preferably, the silicon dioxide layer is formed either by direct sputtering of silicon dioxide or by coating an alkali metal silicate solution on the surface of the reflective metal, optionally containing an aminoalkylsilane and a water-soluble hydroxylated polymer such as a dextran. Alternatively, the reflective support comprises one or more reflective layer units, each reflective layer unit comprising an optically flat layer of silicon, and preferably polysilicon, on a layer of silicon dioxide. Each layer of silicon has a thickness within the range of from 150 to 750 .ANG., from 850 to 1300 .ANG., or from 1700 to 2150 .ANG., and preferably within the range of from 200 to 600 .ANG.. Each layer of silicon dioxide has a thickness within the range of from 800 to 1200 .ANG.. The reflective support is supported on the substantially flat surface of an insoluble support.

    摘要翻译： 反射生物研磨由透明组合物的光学平坦层组成，例如具有第一表面和第二表面的二氧化硅，第一表面上的活性和非活性粘合剂的交替区域以及厚度至少在1000以上的反射金属层 ANGSTROM。 反射金属层可以被支撑在晶片的光学平坦表面上，并且反射金属可以是抛光晶片上的铝，银，金，铬，镍，钛或铂涂层。 优选地，二氧化硅层通过直接溅射二氧化硅或通过在反射金属的表面上涂覆碱金属硅酸盐溶液而形成，任选地含有氨基烷基硅烷和水溶性羟基化聚合物如葡聚糖。 或者，反射支撑件包括一个或多个反射层单元，每个反射层单元在二氧化硅层上包括光学平坦的硅层，优选多晶硅。 每层硅的厚度在150至750安培范围内，从850至1300安培，或1700至2150安培，优选在200至600安培范围内。 每层二氧化硅的厚度在800至1200安培的范围内。 反射支撑体支撑在不溶性支撑体的基本上平坦的表面上。

    Stable allergenic extracts and methods
    3.
    发明授权
    Stable allergenic extracts and methods 失效
    稳定的过敏提取物和方法

    公开(公告)号：US4716120A

    公开(公告)日：1987-12-29

    申请号：US801649

    申请日：1985-11-25

    申请人： Yuh-Geng Tsay ,  Myron A. Beigler ,  Emanuel Calenoff ,  Gerald L. Friesen ,  James L. Nichols

    发明人： Yuh-Geng Tsay ,  Myron A. Beigler ,  Emanuel Calenoff ,  Gerald L. Friesen ,  James L. Nichols

    IPC分类号： A61K39/35 ,  A61K39/00 ,  G01N33/53

    CPC分类号： A61K39/35 ,  Y10S436/809

    摘要： A storage-stable, high potency allergenic extract is prepared by ultrafiltration, retaining fractions having molecular weights of from 1000 to 100,000, and treating the solution with gel polymer and/or carbon absorbents. The extract is dried to a moisture content of less than one weight percent water. The purified solution and aqueous reconstituted solutions of the dried extract provide a transparent, colorless solution which has greatly increased stability, remaining transparent and colorless for extended periods.

    摘要翻译： 通过超滤制备储存稳定的高效力过敏原提取物，保留分子量为1000至100,000的级分，并用凝胶聚合物和/或碳吸收剂处理溶液。 将提取物干燥至含水量小于1重量％的水分。 干燥提取物的纯化溶液和水性重构溶液提供透明的无色溶液，其具有大大增加的稳定性，长时间保持透明和无色。

    Fluorometric assay of chymopapain hypersensitivity and reagents therefor
    4.
    发明授权
    Fluorometric assay of chymopapain hypersensitivity and reagents therefor 失效
    木瓜凝乳蛋白酶超敏反应的荧光测定法及其试剂

    公开(公告)号：US5084379A

    公开(公告)日：1992-01-28

    申请号：US529784

    申请日：1990-05-25

    申请人： Emanuel Calenoff ,  Ruth M. Jones ,  Yuh-Geng Tsay ,  Myron A. Beigler

    发明人： Emanuel Calenoff ,  Ruth M. Jones ,  Yuh-Geng Tsay ,  Myron A. Beigler

    IPC分类号： G01N33/543 ,  G01N33/573 ,  G01N33/58

    CPC分类号： G01N33/543 ,  G01N33/573 ,  G01N33/582

    摘要： A method for identifying and quantifying chymopapain-specific IgE antibody levels in patient serum comprising binding chymopapain-specific IgE, if any, in the serum with chymopapain adhering to an insoluble support, conjugating the serum IgE with a labeled anti-IgE antibody, and measuring the level of labeled compound bound to the insoluble support or in the solution removed therefrom. Special reagents and their manufacture are also disclosed.

    摘要翻译： 一种用于鉴定和定量患者血清中木瓜凝乳蛋白酶原特异性IgE抗体水平的方法，其包括在凝血酶中粘附木瓜凝乳蛋白酶特异性IgE（如果有的话），粘附在不溶性支持物上的木瓜凝乳蛋白酶，将血清IgE与标记的抗IgE抗体缀合， 与不溶性载体结合的标记化合物的水平或从其中除去的溶液中。 还公开了特殊试剂及其制造。

    Fluorometirc enzyme inhibition immunoassay for measuring potency of allergen extracts
    6.
    发明授权
    Fluorometirc enzyme inhibition immunoassay for measuring potency of allergen extracts 失效
    荧光酶抑制免疫测定用于测定变应原提取物的效力

    公开(公告)号：US4528267A

    公开(公告)日：1985-07-09

    申请号：US476440

    申请日：1983-03-17

    申请人： Emanuel Calenoff ,  Yuh-Geng Tsay ,  Ruth M. Jones ,  Scott, John R.

    发明人： Emanuel Calenoff ,  Yuh-Geng Tsay ,  Ruth M. Jones ,  Scott, John R.

    IPC分类号： G01N33/545 ,  G01N33/58 ,  G01N33/54

    CPC分类号： G01N33/582 ,  G01N33/545 ,  Y10S436/809

    摘要： An inhibition assay for measuring the potency of allergen extracts by incubating a mixture of allergen extract and reference allergen specific IgE in a buffered solution with an insoluble support to which reference allergen is adhered. The conjugated IgE adhering to the insoluble support is reacted with an enzyme labeled anti-IgE antibody and the enzyme label is contacted with a solution of a substrate which will yield a fluorescent product in the presence of the enzyme. The level of fluoresence in the solution is measured. The percentage of inhibition of the allergen specific IgE is determined from fluorescence levels measured for various extract concentrations.

    摘要翻译： 通过将缓冲溶液中的变应原提取物和参比变应原特异性IgE的混合物与参与变应原粘附的不溶性载体进行温育来测量变应原提取物的效力的抑制试验。 附着于不溶性载体的缀合的IgE与酶标记的抗IgE抗体反应，并且酶标记物与底物的溶液接触，所述底物将在酶的存在下产生荧光产物。 测量溶液中的荧光水平。 由各种提取物浓度测量的荧光水平确定变应原特异性IgE的抑制百分比。

    Chymopapain allergen and method
    8.
    发明授权
    Chymopapain allergen and method 失效
    木瓜凝乳蛋白酶过敏原和方法

    公开(公告)号：US4499065A

    公开(公告)日：1985-02-12

    申请号：US489898

    申请日：1983-04-29

    申请人： Emanuel Calenoff ,  Ruth M. Jones ,  Yuh-Geng Tsay ,  Myron A. Beigler

    发明人： Emanuel Calenoff ,  Ruth M. Jones ,  Yuh-Geng Tsay ,  Myron A. Beigler

    IPC分类号： A61K38/00 ,  A61K39/35 ,  A61K49/00 ,  C12N9/50 ,  C12N9/99

    CPC分类号： A61K49/0006 ,  A61K39/35 ,  C12N9/50 ,  C12N9/99 ,  A61K38/00

    摘要： A chymopapain derivative having the enzymatic activity reduced by at least 95% while retaining the original allergenic activity of at least 90% is suitable for skin testing to detect allergic hypersensitivity to chymopapain and for treating patients exhibiting allergic hypersensitivity to chymopapain. The enzymatic activity can be blocked by reacting thiol groups of the enzyme with and iodoacetic acid, bromoacetic acid, or a salt, ester or amide derivative thereof, under conditions which block the undesirable enzymatic activity while retaining the desired allergenic activity.

    摘要翻译： 具有降低至少95％的酶活性并且保持至少90％的原始致敏活性的木瓜凝乳蛋白酶衍生物适用于皮肤测试以检测对木瓜凝乳蛋白酶的过敏性超敏反应，并且用于治疗对木瓜凝乳蛋白酶表现出过敏性过敏的患者。 通过使酶的硫醇基与碘乙酸，溴乙酸或其盐，酯或酰胺衍生物在阻碍不期望的酶活性同时保留所需的变应原活性的条件下，可以阻止酶活性。

    Kinetic radioimmunoassay test method and device
    9.
    发明授权
    Kinetic radioimmunoassay test method and device 失效
    动力放射免疫测定方法及装置

    公开(公告)号：US4618485A

    公开(公告)日：1986-10-21

    申请号：US357399

    申请日：1982-03-12

    申请人： Yuh-Geng Tsay ,  Vipin D. Shah

    发明人： Yuh-Geng Tsay ,  Vipin D. Shah

    IPC分类号： G01N33/543 ,  G01N33/557 ,  G01N33/56 ,  G01N33/78

    CPC分类号： G01N33/54306 ,  G01N33/557 ,  Y10S436/804

    摘要： An improved radioimmunoassay test method and device based upon competitive binding test methods wherein immunoreactions are halted at a time when the rate of change of the quantity of bound radiolabeled analyte of interest is inversely proportional to the concentration of analyte of interest in an unknown sera. Based thereon, a test device is created having a single calibration curve 36 which is accurate throughout the shelf life of the device.

    摘要翻译： 一种改进的放射免疫测定方法和装置，其基于竞争性结合试验方法，其中当目标结合的放射性标记的分析物的量的变化速率与未知血清中感兴趣的分析物的浓度成反比时，其中免疫反应停止。 基于此，产生具有单个校准曲线36的测试装置，其在装置的整个保质期内是准确的。