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    Highly reflective biogratings
    1.
    发明授权
    Highly reflective biogratings 失效
    高度反光的生态化

    公开(公告)号：US5478527A

    公开(公告)日：1995-12-26

    申请号：US342486

    申请日：1994-11-21

    申请人： Eric K. Gustafson ,  John Lee ,  Yuh-Geng Tsay

    发明人： Eric K. Gustafson ,  John Lee ,  Yuh-Geng Tsay

    IPC分类号： G01N21/47 ,  G01N33/543 ,  G01N33/553 ,  G01N33/545

    CPC分类号： G01N33/54373 ,  G01N21/4788 ,  G01N2021/7773 ,  Y10S436/805

    摘要： A reflective biograting consists of an optically flat layer of a transparent composition such as silicon dioxide having a first and second surface, alternating zones of active and inactive binding reagent on the first surface, and a reflective metal layer having a thickness of at least above 1000 .ANG.. The reflective metal layer can be supported on an optically flat surface of a wafer, and the reflective metal can be aluminum, silver, gold, chromium, nickel, titanium or platinum coating on a polished wafer. Preferably, the silicon dioxide layer is formed either by direct sputtering of silicon dioxide or by coating an alkali metal silicate solution on the surface of the reflective metal, optionally containing an aminoalkylsilane and a water-soluble hydroxylated polymer such as a dextran. Alternatively, the reflective support comprises one or more reflective layer units, each reflective layer unit comprising an optically flat layer of silicon, and preferably polysilicon, on a layer of silicon dioxide. Each layer of silicon has a thickness within the range of from 150 to 750 .ANG., from 850 to 1300 .ANG., or from 1700 to 2150 .ANG., and preferably within the range of from 200 to 600 .ANG.. Each layer of silicon dioxide has a thickness within the range of from 800 to 1200 .ANG.. The reflective support is supported on the substantially flat surface of an insoluble support.

    摘要翻译： 反射生物研磨由透明组合物的光学平坦层组成，例如具有第一表面和第二表面的二氧化硅，第一表面上的活性和非活性粘合剂的交替区域以及厚度至少在1000以上的反射金属层 ANGSTROM。 反射金属层可以被支撑在晶片的光学平坦表面上，并且反射金属可以是抛光晶片上的铝，银，金，铬，镍，钛或铂涂层。 优选地，二氧化硅层通过直接溅射二氧化硅或通过在反射金属的表面上涂覆碱金属硅酸盐溶液而形成，任选地含有氨基烷基硅烷和水溶性羟基化聚合物如葡聚糖。 或者，反射支撑件包括一个或多个反射层单元，每个反射层单元在二氧化硅层上包括光学平坦的硅层，优选多晶硅。 每层硅的厚度在150至750安培范围内，从850至1300安培，或1700至2150安培，优选在200至600安培范围内。 每层二氧化硅的厚度在800至1200安培的范围内。 反射支撑体支撑在不溶性支撑体的基本上平坦的表面上。

    DNA probe diffraction assay and reagents
    2.
    发明授权
    DNA probe diffraction assay and reagents 失效
    DNA探针衍生物测定和试剂

    公开(公告)号：US5089387A

    公开(公告)日：1992-02-18

    申请号：US216691

    申请日：1988-07-07

    申请人： Yuh-Geng Tsay ,  Emanuel Calenoff ,  Eric K. Gustafson ,  Rick Trebino ,  John Lee

    发明人： Yuh-Geng Tsay ,  Emanuel Calenoff ,  Eric K. Gustafson ,  Rick Trebino ,  John Lee

    IPC分类号： G01N21/47 ,  C12Q1/68 ,  G01N21/77 ,  G01N33/543 ,  G02B5/18

    CPC分类号： G01N21/4788 ,  C12Q1/6825 ,  G01N33/54373 ,  G01N2021/757

    摘要： The assay of the subject invention uses DNA sequences as probes in a nucleic acid hybridization diffraction assay, to detect specific DNA sequences in a sample. Diffraction assay methodologies are applied to determine the presence and amount of analyte.This invention involves a discovery in the areas of supporting surfaces for a biogrid or biograting which provide greatly reduced non-specific hybridization and binding. A preferred process of this invention involves manufacturing a biograting for use in a light diffraction assay, and comprises adhering a uniform layer of hybridizing reagent comprising a nucleotide sequence on a smooth, solid surface and exposing the surface to UV radiation through a shadow mask with a diffraction grating pattern of lines to selectively deactivate the hybridizing reagent, leaving a biological diffraction grating design of lines of active hybridizing reagent. The smooth, solid surface is preferably selected from the group consisting of polysilicon and single crystalline silicon surfaces.The diffraction hybridizing assay method of this invention for determining the presence or quantity of an analyte in an aqueous sample comprises contacting a nucleic acid sequence diffraction biogrid with the sample under proper circumstances and for a sufficient time to permit nucleic acid hybridization between a nucleic acid sequence probe and an analyte; separating the biogrid from the sample; illuminating the biogrid with light from a light source; and determining the light diffracted by the diffraction hybridization assay surface.

    Fluorometric assay of chymopapain hypersensitivity and reagents therefor
    3.
    发明授权
    Fluorometric assay of chymopapain hypersensitivity and reagents therefor 失效
    木瓜凝乳蛋白酶超敏反应的荧光测定法及其试剂

    公开(公告)号：US5084379A

    公开(公告)日：1992-01-28

    申请号：US529784

    申请日：1990-05-25

    申请人： Emanuel Calenoff ,  Ruth M. Jones ,  Yuh-Geng Tsay ,  Myron A. Beigler

    发明人： Emanuel Calenoff ,  Ruth M. Jones ,  Yuh-Geng Tsay ,  Myron A. Beigler

    IPC分类号： G01N33/543 ,  G01N33/573 ,  G01N33/58

    CPC分类号： G01N33/543 ,  G01N33/573 ,  G01N33/582

    摘要： A method for identifying and quantifying chymopapain-specific IgE antibody levels in patient serum comprising binding chymopapain-specific IgE, if any, in the serum with chymopapain adhering to an insoluble support, conjugating the serum IgE with a labeled anti-IgE antibody, and measuring the level of labeled compound bound to the insoluble support or in the solution removed therefrom. Special reagents and their manufacture are also disclosed.

    摘要翻译： 一种用于鉴定和定量患者血清中木瓜凝乳蛋白酶原特异性IgE抗体水平的方法，其包括在凝血酶中粘附木瓜凝乳蛋白酶特异性IgE（如果有的话），粘附在不溶性支持物上的木瓜凝乳蛋白酶，将血清IgE与标记的抗IgE抗体缀合， 与不溶性载体结合的标记化合物的水平或从其中除去的溶液中。 还公开了特殊试剂及其制造。

    Kinetic radioimmunoassay test method and device
    4.
    发明授权
    Kinetic radioimmunoassay test method and device 失效
    动力放射免疫测定方法及装置

    公开(公告)号：US4618485A

    公开(公告)日：1986-10-21

    申请号：US357399

    申请日：1982-03-12

    申请人： Yuh-Geng Tsay ,  Vipin D. Shah

    发明人： Yuh-Geng Tsay ,  Vipin D. Shah

    IPC分类号： G01N33/543 ,  G01N33/557 ,  G01N33/56 ,  G01N33/78

    CPC分类号： G01N33/54306 ,  G01N33/557 ,  Y10S436/804

    摘要： An improved radioimmunoassay test method and device based upon competitive binding test methods wherein immunoreactions are halted at a time when the rate of change of the quantity of bound radiolabeled analyte of interest is inversely proportional to the concentration of analyte of interest in an unknown sera. Based thereon, a test device is created having a single calibration curve 36 which is accurate throughout the shelf life of the device.

    摘要翻译： 一种改进的放射免疫测定方法和装置，其基于竞争性结合试验方法，其中当目标结合的放射性标记的分析物的量的变化速率与未知血清中感兴趣的分析物的浓度成反比时，其中免疫反应停止。 基于此，产生具有单个校准曲线36的测试装置，其在装置的整个保质期内是准确的。

    Method and kit for enzymatically determining the pH of a specimen
    6.
    发明授权
    Method and kit for enzymatically determining the pH of a specimen 失效
    用于酶法测定样品pH值的方法和试剂盒

    公开(公告)号：US5595867A

    公开(公告)日：1997-01-21

    申请号：US452171

    申请日：1995-05-26

    申请人： Cheng-I Lin ,  Yuh-Geng Tsay

    发明人： Cheng-I Lin ,  Yuh-Geng Tsay

    IPC分类号： C12Q1/00 ,  C12Q1/58 ,  C12Q1/62

    CPC分类号： C12Q1/62 ,  C12Q1/00 ,  C12Q1/58

    摘要： The present invention concerns a method of enzymatically determining the pH of a specimen (e.g., a solution or a biological fluid) and a kit for conducting the method. The present method involves mixing (1) a specimen with (2) an enzyme and (3) one or more substrates for the enzyme in a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen; determining the activity of the enzyme; and correlating the activity of the enzyme to the pH of the specimen. Each of the sample, the enzyme, the substrate and the buffered solution is present in an amount effective to provide the direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present kit contains an enzyme, one or more substrates for the enzyme present in an amount effective to determine the enzyme activity, and a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present invention is particularly applicable to detecting adulteration of a urine specimen.

    摘要翻译： 本发明涉及一种酶法测定样品（例如溶液或生物流体）的pH值的方法和用于进行该方法的试剂盒。 本发明的方法包括将样品与（2）酶和（3）酶的一种或多种底物混合（1）在具有pH的缓冲溶液中，以提供酶的活性与pH之间的直接比例关系 的样本; 测定酶的活性; 并将酶的活性与样品的pH相关联。 每个样品，酶，底物和缓冲溶液以有效提供酶的活性和样品的pH之间的直接比例关系的量存在。 本试剂盒包含一种酶，一种或多种用于确定酶活性的量存在的酶的底物，以及具有有效提供酶的活性和pH的直接比例关系的pH的缓冲溶液 标本。 本发明特别适用于检测尿标本的掺假。

    Method and kit for enzymatically determining the pH of a specimen
    7.
    发明授权
    Method and kit for enzymatically determining the pH of a specimen 失效
    用于酶法测定样品pH值的方法和试剂盒

    公开(公告)号：US5567581A

    公开(公告)日：1996-10-22

    申请号：US398693

    申请日：1995-03-06

    申请人： Cheng-I Lin ,  Yuh-Geng Tsay

    发明人： Cheng-I Lin ,  Yuh-Geng Tsay

    IPC分类号： C12Q1/00 ,  C12Q1/58 ,  C12Q1/62 ,  C12Q1/26 ,  C12Q1/54

    CPC分类号： C12Q1/62 ,  C12Q1/00 ,  C12Q1/58

    摘要： The present invention concerns a method of enzymatically determining the pH of a specimen (e.g., a solution or a biological fluid) and a kit for conducting the method. The present method involves mixing (1) a specimen with (2) an enzyme and (3) one or more substrates for the enzyme in a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen; determining the activity of the enzyme; and correlating the activity of the enzyme to the pH of the specimen. Each of the sample, the enzyme, the substrate and the buffered solution is present in an amount effective to provide the direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present kit contains an enzyme, one or more substrates for the enzyme present in an amount effective to determine the enzyme activity, and a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present invention is particularly applicable to detecting adulteration of a urine specimen.

    摘要翻译： 本发明涉及一种酶法测定样品（例如溶液或生物流体）的pH值的方法和用于进行该方法的试剂盒。 本发明的方法包括将样品与（2）酶和（3）酶的一种或多种底物混合（1）在具有pH的缓冲溶液中，以提供酶的活性与pH之间的直接比例关系 的样本; 测定酶的活性; 并将酶的活性与样品的pH相关联。 每个样品，酶，底物和缓冲溶液以有效提供酶的活性和样品的pH之间的直接比例关系的量存在。 本试剂盒包含一种酶，一种或多种用于确定酶活性的量存在的酶的底物，以及具有有效提供酶的活性和pH的直接比例关系的pH的缓冲溶液 标本。 本发明特别适用于检测尿标本的掺假。

    Stable allergenic extracts and methods
    8.
    发明授权
    Stable allergenic extracts and methods 失效
    稳定的过敏提取物和方法

    公开(公告)号：US4716120A

    公开(公告)日：1987-12-29

    申请号：US801649

    申请日：1985-11-25

    申请人： Yuh-Geng Tsay ,  Myron A. Beigler ,  Emanuel Calenoff ,  Gerald L. Friesen ,  James L. Nichols

    发明人： Yuh-Geng Tsay ,  Myron A. Beigler ,  Emanuel Calenoff ,  Gerald L. Friesen ,  James L. Nichols

    IPC分类号： A61K39/35 ,  A61K39/00 ,  G01N33/53

    CPC分类号： A61K39/35 ,  Y10S436/809

    摘要： A storage-stable, high potency allergenic extract is prepared by ultrafiltration, retaining fractions having molecular weights of from 1000 to 100,000, and treating the solution with gel polymer and/or carbon absorbents. The extract is dried to a moisture content of less than one weight percent water. The purified solution and aqueous reconstituted solutions of the dried extract provide a transparent, colorless solution which has greatly increased stability, remaining transparent and colorless for extended periods.

    摘要翻译： 通过超滤制备储存稳定的高效力过敏原提取物，保留分子量为1000至100,000的级分，并用凝胶聚合物和/或碳吸收剂处理溶液。 将提取物干燥至含水量小于1重量％的水分。 干燥提取物的纯化溶液和水性重构溶液提供透明的无色溶液，其具有大大增加的稳定性，长时间保持透明和无色。

    Quinonoid intermediates from chloroprene
    9.
    发明授权
    Quinonoid intermediates from chloroprene 失效
    喹诺酮类中间体

    公开(公告)号：US4164503A

    公开(公告)日：1979-08-14

    申请号：US949511

    申请日：1978-10-10

    申请人： Andrew S. Kende ,  Yuh-Geng Tsay ,  Takuya Furuta

    发明人： Andrew S. Kende ,  Yuh-Geng Tsay ,  Takuya Furuta

    IPC分类号： C07C50/26 ,  C07C49/72 ,  C07C49/74 ,  C07C49/80 ,  C09B3/82

    CPC分类号： C07C50/26

    摘要： There is provided a novel and efficent method of synthesizing 7,10-dihydro-6,11-dihydroxy-5,9,12(8H)-naphthacenetrione and analogs thereof substituted in the 1,2,3 or 4 positions. The novel method comprises reacting the appropriate quinizarinquinone with a haloprene under Diels-Alder conditions, followed by aromatization and treatment with strong acid under very mild temperature conditions. The products of this process are important intermediates in the synthesis of daunomycin and its analogs which are useful in the therapy of neoplastic ailments.

    摘要翻译： 提供了合成7,10-二氢-6,11-二羟基-5,9,12（8H） - 萘乙酮及其在1,2,3或4位取代的其类似物的新颖有效的方法。 该新方法包括在Diels-Alder条件下使合适的醌茜醌与卤代丁烯反应，然后在非常温和的温度条件下用强酸进行芳构化和处理。 该过程的产物是道诺霉素及其类似物的合成中的重要中间体，其可用于治疗肿瘤性疾病。