公开(公告)号：US20070045118A1

公开(公告)日：2007-03-01

申请号：US11510838

申请日：2006-08-28

申请人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

发明人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

IPC分类号： G01N27/00

CPC分类号： G01N27/44778

摘要： The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.

摘要翻译： 本发明提供一种在样品分离装置中使用的样品分离装置，其包括：保持装置，用于保持支撑第一介质的第一介质支持体; 以及用于在平行或垂直于其侧面沿第一方向和第二方向延伸的平面的方向上移动固定装置或保持装置的驱动装置。 样品分离装置包括用于存储第二介质的绝缘体，其允许在第一方向上在第一介质中分离的样品在与第一方向不同的第二方向上进一步分离，其中：绝缘体包括第一开口和第二开口 其中每个开口限定第二介质带电的第二方向，第二开口具有允许包含分离样品的第一介质沿着第一方向附着到第二介质的形状。 本发明实现了二维电泳的自动化，以增强二维电泳的便利性，较少依赖于操作者的技能，并提高电泳结果的再现性。

公开(公告)号：US07964077B2

公开(公告)日：2011-06-21

申请号：US11510838

申请日：2006-08-28

申请人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

发明人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： G01N27/44778

摘要： The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.

摘要翻译： 本发明提供一种在样品分离装置中使用的样品分离装置，其包括：保持装置，用于保持支撑第一介质的第一介质支持体; 以及用于在平行或垂直于其侧面沿第一方向和第二方向延伸的平面的方向上移动固定装置或保持装置的驱动装置。 样品分离装置包括用于存储第二介质的绝缘体，其允许在第一方向上在第一介质中分离的样品在与第一方向不同的第二方向上进一步分离，其中：绝缘体包括第一开口和第二开口 其中每个开口限定第二介质带电的第二方向，第二开口具有允许包含分离样品的第一介质沿着第一方向附着到第二介质的形状。 本发明实现了二维电泳的自动化，以增强二维电泳的便利性，较少依赖于操作者的技能，并提高电泳结果的再现性。

公开(公告)号：US08062492B2

公开(公告)日：2011-11-22

申请号：US12090789

申请日：2006-10-18

申请人： Yoshio Suzuki ,  Kenji Yokoyama ,  Ichiji Namatame

发明人： Yoshio Suzuki ,  Kenji Yokoyama ,  Ichiji Namatame

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44726

摘要： A method for analyzing proteins with the use of electrophoresis is provided, which makes it possible to rapidly and conveniently analyze a great variety of proteins with high sensitivity. The method for analyzing a protein in a sample comprises setting a sample in a carrier for electrophoresis, performing electrophoresis for the sample using a buffer for electrophoresis in which a labeling compound represented by formula I: wherein R1 is an aryl group or a heteroaryl group that may be substituted, R2 is a heterocyclic group that may be substituted, and n is an integer between 1 and 5, is dissolved, and then detecting a complex of the compound of formula I and the protein via spectrophotometrical measurement.

摘要翻译： 提供了一种利用电泳分析蛋白质的方法，可以快速方便地分析高灵敏度的多种蛋白质。 用于分析样品中的蛋白质的方法包括设置用于电泳的载体中的样品，使用电泳缓冲液对样品进行电泳，其中由式I表示的标记化合物：其中R1是芳基或杂芳基， 可以被取代，R2是可以被取代的杂环基，n是1和5之间的整数，并且然后通过分光光度测量法检测式I化合物和蛋白质的络合物。

公开(公告)号：US20090045060A1

公开(公告)日：2009-02-19

申请号：US12090789

申请日：2006-10-18

申请人： Yoshio Suzuki ,  Kenji Yokoyama ,  Ichiji Namatame

发明人： Yoshio Suzuki ,  Kenji Yokoyama ,  Ichiji Namatame

IPC分类号： G01N27/26

CPC分类号： G01N27/44726

摘要： A method for analyzing proteins with the use of electrophoresis is provided, which makes it possible to rapidly and conveniently analyze a great variety of proteins with high sensitivity. The method for analyzing a protein in a sample comprises setting a sample in a carrier for electrophoresis, performing electrophoresis for the sample using a buffer for electrophoresis in which a labeling compound represented by formula I: wherein R1 is an aryl group or a heteroaryl group that may be substituted, R2 is a heterocyclic group that may be substituted, and n is an integer between 1 and 5, is dissolved, and then detecting a complex of the compound of formula I and the protein via spectrophotometrical measurement.

摘要翻译： 提供了一种利用电泳分析蛋白质的方法，可以快速方便地分析高灵敏度的多种蛋白质。 用于分析样品中的蛋白质的方法包括设置用于电泳的载体中的样品，使用电泳缓冲液对样品进行电泳，其中由式I表示的标记化合物：其中R1是芳基或杂芳基， 可以被取代，R2是可以被取代的杂环基，n是1和5之间的整数，并且然后通过分光光度测量法检测式I化合物和蛋白质的络合物。

公开(公告)号：US5221494A

公开(公告)日：1993-06-22

申请号：US582847

申请日：1990-10-15

申请人： Masanori Ikeda ,  Hiroyuki Fukui ,  Yoshio Suzuki

发明人： Masanori Ikeda ,  Hiroyuki Fukui ,  Yoshio Suzuki

IPC分类号： C10M105/54 ,  C10M105/56 ,  C10M105/76 ,  C10M107/38 ,  C10M107/40 ,  C10M107/46 ,  C10M107/50 ,  C10M111/00 ,  C10M171/00

CPC分类号： C10M107/50 ,  C10M105/54 ,  C10M105/56 ,  C10M105/76 ,  C10M107/38 ,  C10M107/40 ,  C10M107/46 ,  C10M111/00 ,  C10M171/008 ,  C10M2211/0206 ,  C10M2211/022 ,  C10M2211/0225 ,  C10M2211/0245 ,  C10M2211/042 ,  C10M2211/044 ,  C10M2211/06 ,  C10M2213/00 ,  C10M2213/023 ,  C10M2213/04 ,  C10M2213/043 ,  C10M2213/06 ,  C10M2213/0606 ,  C10M2213/0623 ,  C10M2215/003 ,  C10M2215/042 ,  C10M2215/08 ,  C10M2215/082 ,  C10M2215/16 ,  C10M2215/222 ,  C10M2215/24 ,  C10M2215/28 ,  C10M2217/00 ,  C10M2217/003 ,  C10M2217/02 ,  C10M2217/04 ,  C10M2219/085 ,  C10M2221/00 ,  C10M2227/025 ,  C10M2227/045 ,  C10M2229/025 ,  C10M2229/0405 ,  C10M2229/0415 ,  C10M2229/0425 ,  C10M2229/0435 ,  C10M2229/0445 ,  C10M2229/0455 ,  C10M2229/0465 ,  C10M2229/0475 ,  C10M2229/0485 ,  C10M2229/0505 ,  C10M2229/051 ,  C10M2229/0515 ,  C10M2229/052 ,  C10M2229/0525 ,  C10M2229/0535 ,  C10M2229/0545 ,  C10N2240/00 ,  C10N2240/22 ,  C10N2240/30 ,  C10N2240/50 ,  C10N2240/52 ,  C10N2240/54 ,  C10N2240/56 ,  C10N2240/58 ,  C10N2240/60 ,  C10N2240/66

摘要： A lubricant for use in a refrigeration system using a tetrafluoroethane refrigerant, which comprises a fluorine-containing compound represented by the following formula: ##STR1## wherein X represents a multiple bond-containing monovalent group, and A represents a mono-, bi- or trivalent unsubstituted or partially substituted perfluorocarbon residue, a mono, bi- or trivalent unsubstituted or partially substituted perfluoroether residue or a mono-, bi- or trivalent unsubstituted or partially substituted perfluoropolyether residue.This lubricant has a good miscibility with a tetrafluoroethane refrigerant as represented by HFC-134a over a wide range of temperatures ranging from the low temperature to the high temperature and is excellent in properties, such as heat resistance, lubricating properties, electrically insulating properties and viscosity-temperature characteristics.

公开(公告)号：US08692047B2

公开(公告)日：2014-04-08

申请号：US13364754

申请日：2012-02-02

申请人： Satoshi Koide ,  Kenji Yokoyama ,  Yoshio Suzuki

发明人： Satoshi Koide ,  Kenji Yokoyama ,  Yoshio Suzuki

IPC分类号： C07C7/12

CPC分类号： B01D15/363 ,  B01D15/325 ,  G01N30/14 ,  G01N33/493 ,  G01N2030/009

摘要： A method of purifying 8-isoprostane is provided that includes a step of contacting a liquid sample containing 8-isoprostane with an ion exchange support having a quaternary ammonium salt immobilized thereon such that 8-isoprostane in the liquid sample is retained on the ion exchange support and a step of eluting 8-isoprostane from the ion exchange support using a first eluent containing a water-soluble organic solvent and water as main components.

摘要翻译： 提供了一种纯化8-异前列烷的方法，其包括使含有8-异前列烷烷的液体样品与固定有季铵盐的离子交换载体接触的步骤，使得液体样品中的8-异前列烷保留在离子交换载体上 以及使用包含水溶性有机溶剂和水作为主要成分的第一洗脱液从离子交换载体洗脱8-异前列烷的步骤。

公开(公告)号：US08652850B2

公开(公告)日：2014-02-18

申请号：US13364822

申请日：2012-02-02

申请人： Satoshi Koide ,  Kenji Yokoyama ,  Yoshio Suzuki

发明人： Satoshi Koide ,  Kenji Yokoyama ,  Yoshio Suzuki

IPC分类号： G01N21/64

CPC分类号： G01N33/582 ,  C09B57/00 ,  G01N33/6809 ,  G01N2030/8813

摘要： A method of quantitatively determining 8-isoprostane is provided that includes fluorescently labeling 8-isoprostane with a quinoxalinone derivative in an excess amount relative to 8-isoprostane, separating a fluorescently labeled 8-isoprostane from a unreacted quinoxalinone derivative by contacting a reaction mixture containing the fluorescently labeled 8-isoprostane and the unreacted quinoxalinone derivative with a cation exchange support having a sulfonic acid or a sulfonate immobilized thereon, and quantitatively determining the fluorescently labeled 8-isoprostane that has been separated from the unreacted quinoxalinone derivative.

摘要翻译： 提供了一种定量测定8-异前列烷烷的方法，其包括相对于8-异前列烷荧光标记8-异前列烷与过量的喹喔啉酮衍生物，从未反应的喹喔啉酮衍生物中分离出荧光标记的8-异前列烷烷， 荧光标记的8-异雄甾烷和未反应的喹喔啉酮衍生物与其上固定有磺酸或磺酸的阳离子交换载体，并定量测定已从未反应的喹喔啉酮衍生物中分离的荧光标记的8-异雄甾烷。

公开(公告)号：US20120220050A1

公开(公告)日：2012-08-30

申请号：US13364754

申请日：2012-02-02

申请人： Satoshi Koide ,  Kenji Yokoyama ,  Yoshio Suzuki

发明人： Satoshi Koide ,  Kenji Yokoyama ,  Yoshio Suzuki

IPC分类号： G01N33/53 ,  C07C7/12 ,  G01N21/64

CPC分类号： B01D15/363 ,  B01D15/325 ,  G01N30/14 ,  G01N33/493 ,  G01N2030/009

摘要： A method of purifying 8-isoprostane is provided that includes a step of contacting a liquid sample containing 8-isoprostane with an ion exchange support having a quaternary ammonium salt immobilized thereon such that 8-isoprostane in the liquid sample is retained on the ion exchange support and a step of eluting 8-isoprostane from the ion exchange support using a first eluent containing a water-soluble organic solvent and water as main components.

摘要翻译： 提供了一种纯化8-异前列烷的方法，其包括使含有8-异前列烷烷的液体样品与固定有季铵盐的离子交换载体接触的步骤，使得液体样品中的8-异前列烷保留在离子交换载体上 以及使用包含水溶性有机溶剂和水作为主要成分的第一洗脱液从离子交换载体洗脱8-异前列烷的步骤。

公开(公告)号：US08263791B2

公开(公告)日：2012-09-11

申请号：US12492583

申请日：2009-06-26

申请人： Yoshio Suzuki ,  Kenji Yokoyama ,  Nobuyuki Takagi ,  Tomoyuki Chimuro ,  Atsushi Shinohara

发明人： Yoshio Suzuki ,  Kenji Yokoyama ,  Nobuyuki Takagi ,  Tomoyuki Chimuro ,  Atsushi Shinohara

IPC分类号： C07D313/00

CPC分类号： C07D309/34

摘要： A convenient, rapid, and highly sensitive protein detection method is provided. According to the present invention, an easily water-soluble compound of formula I is provided: [wherein, R1 and R2 are same or different, each of which is an aryl or heteroaryl group substituted with one or more anionic substituents].

摘要翻译： 提供了一种方便，快速，高灵敏度的蛋白质检测方法。 根据本发明，提供了一种易于水溶性的式I化合物：[其中，R 1和R 2相同或不同，各自为被一个或多个阴离子取代基取代的芳基或杂芳基]。

公开(公告)号：US4693637A

公开(公告)日：1987-09-15

申请号：US801847

申请日：1985-11-26

申请人： Yoshio Suzuki ,  Yoshio Suzuki ,  Satoshi Saitoh ,  Mituoki Yamamoto ,  Hisashi Hosomi

发明人： Yoshio Suzuki ,  Yoshio Suzuki ,  Satoshi Saitoh ,  Mituoki Yamamoto ,  Hisashi Hosomi

IPC分类号： E02B17/00 ,  E02B17/02

CPC分类号： E02B17/025 ,  E02B2017/0065 ,  E02B2017/0069

摘要： The present invention relates to a gravity type oceanic structure and its stable installation wherein a skirt is provided downward on the outside periphery of the lower end of the part to be settled and fixed on the sea bed. When the structure is settled on the sea bed, the skirt is pushed into the sea bed foundation to intercept penetration of sea water from the circumference into the structure, and sea water in the skirt is adequately discharged by draining means to make the internal pressure equal to the atmospheric pressure. Buoyancy is thereby eliminated so that the bottom surface of the structure is fixed firmly to the sea bed.

摘要翻译： 本发明涉及一种重力型海洋结构及其稳定的装置，其中裙部设置在待沉降和固定在海床上的部分的下端的外周边上。 当结构安置在海床上时，裙子被推入海床基础，以拦截海水从周边渗透进入结构，并且通过排水装置将裙带中的海水充分排放，以使内部压力相等 到大气压力。 由此消除浮力，使结构的底面牢固地固定在海床上。