利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

6 条记录 (耗时 0.008 秒)

    Electrophoresis Cassette and Method for Producing the Same
    2.
    发明申请
    Electrophoresis Cassette and Method for Producing the Same 审中-公开
    电泳盒及其制备方法

    公开(公告)号：US20090314643A1

    公开(公告)日：2009-12-24

    申请号：US12224337

    申请日：2007-02-28

    申请人： Koji Sakairi ,  Chie Hayashida ,  Ichiji Namatame ,  Kenji Yokoyama ,  Atsunori Hiratsuka ,  Kisho Shiseki

    发明人： Koji Sakairi ,  Chie Hayashida ,  Ichiji Namatame ,  Kenji Yokoyama ,  Atsunori Hiratsuka ,  Kisho Shiseki

    IPC分类号： C25B9/00 ,  C23C16/513

    CPC分类号： G01N27/44704

    摘要： The object of the present invention is to inject smoothly a precursor to be a support and make an excellent support in which a boundary between a separation gel and a concentration gel after the injection is not ill-defined in the cassette which is made by combining two plates made of a synthetic resin, and in order to achieve the object, the present invention provides an electrophoresis cassette made by combining plates made of a synthetic resin which has a cavity for forming a support, wherein the surface of the plate which contacts with the support is covered with a SiOx film, and the SiOx film has a contact angle to water of 30° or less, and preferably 10° or less.

    摘要翻译： 本发明的目的是平滑地注入作为载体的前体，并且制成优异的载体，其中注射后的分离凝胶和浓缩凝胶之间的边界在通过组合两个 为了达到上述目的，本发明提供一种电泳盒，其通过将由合成树脂制成的板组合而形成，该合成树脂具有用于形成支撑体的空腔，其中该板的表面与 支撑体覆盖有SiO x膜，SiO x膜与水的接触角为30°以下，优选为10°以下。

    Method for analyzing protein
    3.
    发明授权
    Method for analyzing protein 有权
    分析蛋白质的方法

    公开(公告)号：US08062492B2

    公开(公告)日：2011-11-22

    申请号：US12090789

    申请日：2006-10-18

    申请人： Yoshio Suzuki ,  Kenji Yokoyama ,  Ichiji Namatame

    发明人： Yoshio Suzuki ,  Kenji Yokoyama ,  Ichiji Namatame

    IPC分类号： G01N27/447 ,  G01N27/453

    CPC分类号： G01N27/44726

    摘要： A method for analyzing proteins with the use of electrophoresis is provided, which makes it possible to rapidly and conveniently analyze a great variety of proteins with high sensitivity. The method for analyzing a protein in a sample comprises setting a sample in a carrier for electrophoresis, performing electrophoresis for the sample using a buffer for electrophoresis in which a labeling compound represented by formula I: wherein R1 is an aryl group or a heteroaryl group that may be substituted, R2 is a heterocyclic group that may be substituted, and n is an integer between 1 and 5, is dissolved, and then detecting a complex of the compound of formula I and the protein via spectrophotometrical measurement.

    摘要翻译： 提供了一种利用电泳分析蛋白质的方法，可以快速方便地分析高灵敏度的多种蛋白质。 用于分析样品中的蛋白质的方法包括设置用于电泳的载体中的样品，使用电泳缓冲液对样品进行电泳，其中由式I表示的标记化合物：其中R1是芳基或杂芳基， 可以被取代，R2是可以被取代的杂环基，n是1和5之间的整数，并且然后通过分光光度测量法检测式I化合物和蛋白质的络合物。

    METHOD OF SEPARATING PROTEIN, METHOD OF STAINING PROTEIN AND LIQUID PROTEIN-STAINING AGENT AND PROTEIN-STAINING KIT TO BE USED IN THESE METHODS
    4.
    发明申请
    METHOD OF SEPARATING PROTEIN, METHOD OF STAINING PROTEIN AND LIQUID PROTEIN-STAINING AGENT AND PROTEIN-STAINING KIT TO BE USED IN THESE METHODS 审中-公开
    分离蛋白质的方法，蛋白质和液体蛋白质染色剂的染色方法以及用于这些方法的蛋白质染色试剂盒

    公开(公告)号：US20090223823A1

    公开(公告)日：2009-09-10

    申请号：US11720927

    申请日：2005-12-06

    申请人： Ichiji Namatame ,  Yoshinori Ishii ,  Yoshimasa Saito ,  Takayoshi Komatsu ,  Yasuhiro Ogawa ,  Takashi Shibata ,  Hideki Kinoshita ,  Kenji Yokoyama

    发明人： Ichiji Namatame ,  Yoshinori Ishii ,  Yoshimasa Saito ,  Takayoshi Komatsu ,  Yasuhiro Ogawa ,  Takashi Shibata ,  Hideki Kinoshita ,  Kenji Yokoyama

    IPC分类号： B01D57/02 ,  C07K2/00 ,  G01N33/00

    CPC分类号： G01N27/44726

    摘要： A method of separating a protein by gel electrophoresis, which comprises a staining step for bringing a protein-containing sample into contact with a staining liquid containing a staining agent, a surfactant and a buffer solution, and an electrophoresis step for subjecting the protein-containing sample after the staining step to gel electrophoresis.According to the present invention, using a staining liquid containing a surfactant such as SDS and the like, a protein-containing sample can be stained in a short time, and can develop color with high sensitivity. In addition, since an excess staining agent migrates earlier than protein by electrophoresis, a washing operation is not necessary. Furthermore, by staining, after the first dimension electrophoresis, with a staining liquid containing a surfactant such as SDS and the like, the second dimension electrophoresis can be performed immediately. As a result, the number of steps can be reduced as compared to conventional protein separation methods, and the separation operation can be simplified. Hence, a method capable of staining and separating a protein conveniently and quickly by electrophoresis can be provided.

    摘要翻译： 一种通过凝胶电泳分离蛋白质的方法，其包括使含蛋白质的样品与含有染色剂，表面活性剂和缓冲溶液的染色液体接触的染色步骤，以及将含蛋白质的样品 染色步骤后进行凝胶电泳。 根据本发明，使用含有SDS等表面活性剂的染色液，可以在短时间内对含蛋白质的样品进行染色，可以以高灵敏度显色。 此外，由于过量的染色剂通过电泳比蛋白质迁移更早，所以不需要洗涤操作。 此外，通过染色，在第一维电泳之后，用含有诸如SDS等的表面活性剂的染色液进行染色，可以立即进行第二维电泳。 结果，与常规蛋白质分离方法相比，可以减少步骤数，并且可以简化分离操作。 因此，可以提供通过电泳方便快速地染色和分离蛋白质的方法。

    METHOD FOR ANALYZING PROTEIN
    6.
    发明申请
    METHOD FOR ANALYZING PROTEIN 有权
    分析蛋白质的方法

    公开(公告)号：US20090045060A1

    公开(公告)日：2009-02-19

    申请号：US12090789

    申请日：2006-10-18

    申请人： Yoshio Suzuki ,  Kenji Yokoyama ,  Ichiji Namatame

    发明人： Yoshio Suzuki ,  Kenji Yokoyama ,  Ichiji Namatame

    IPC分类号： G01N27/26

    CPC分类号： G01N27/44726

    摘要： A method for analyzing proteins with the use of electrophoresis is provided, which makes it possible to rapidly and conveniently analyze a great variety of proteins with high sensitivity. The method for analyzing a protein in a sample comprises setting a sample in a carrier for electrophoresis, performing electrophoresis for the sample using a buffer for electrophoresis in which a labeling compound represented by formula I: wherein R1 is an aryl group or a heteroaryl group that may be substituted, R2 is a heterocyclic group that may be substituted, and n is an integer between 1 and 5, is dissolved, and then detecting a complex of the compound of formula I and the protein via spectrophotometrical measurement.

    摘要翻译： 提供了一种利用电泳分析蛋白质的方法，可以快速方便地分析高灵敏度的多种蛋白质。 用于分析样品中的蛋白质的方法包括设置用于电泳的载体中的样品，使用电泳缓冲液对样品进行电泳，其中由式I表示的标记化合物：其中R1是芳基或杂芳基， 可以被取代，R2是可以被取代的杂环基，n是1和5之间的整数，并且然后通过分光光度测量法检测式I化合物和蛋白质的络合物。