公开(公告)号：US20070045118A1

公开(公告)日：2007-03-01

申请号：US11510838

申请日：2006-08-28

申请人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

发明人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

IPC分类号： G01N27/00

CPC分类号： G01N27/44778

摘要： The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.

摘要翻译： 本发明提供一种在样品分离装置中使用的样品分离装置，其包括：保持装置，用于保持支撑第一介质的第一介质支持体; 以及用于在平行或垂直于其侧面沿第一方向和第二方向延伸的平面的方向上移动固定装置或保持装置的驱动装置。 样品分离装置包括用于存储第二介质的绝缘体，其允许在第一方向上在第一介质中分离的样品在与第一方向不同的第二方向上进一步分离，其中：绝缘体包括第一开口和第二开口 其中每个开口限定第二介质带电的第二方向，第二开口具有允许包含分离样品的第一介质沿着第一方向附着到第二介质的形状。 本发明实现了二维电泳的自动化，以增强二维电泳的便利性，较少依赖于操作者的技能，并提高电泳结果的再现性。

公开(公告)号：US07964077B2

公开(公告)日：2011-06-21

申请号：US11510838

申请日：2006-08-28

申请人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

发明人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： G01N27/44778

摘要： The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.

摘要翻译： 本发明提供一种在样品分离装置中使用的样品分离装置，其包括：保持装置，用于保持支撑第一介质的第一介质支持体; 以及用于在平行或垂直于其侧面沿第一方向和第二方向延伸的平面的方向上移动固定装置或保持装置的驱动装置。 样品分离装置包括用于存储第二介质的绝缘体，其允许在第一方向上在第一介质中分离的样品在与第一方向不同的第二方向上进一步分离，其中：绝缘体包括第一开口和第二开口 其中每个开口限定第二介质带电的第二方向，第二开口具有允许包含分离样品的第一介质沿着第一方向附着到第二介质的形状。 本发明实现了二维电泳的自动化，以增强二维电泳的便利性，较少依赖于操作者的技能，并提高电泳结果的再现性。

公开(公告)号：US20110114485A1

公开(公告)日：2011-05-19

申请号：US13054271

申请日：2009-07-15

申请人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

发明人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44773

摘要： An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.

摘要翻译： 电泳装置包括样品分离部分，用于容纳用于在水平方向上分离样品的样品分离介质，样品分离部分包含样品分离介质，使得样品分离介质具有暴露部分，至少一端 所述样品分离介质的表面与所述水平方向平行; 以及介质连接装置，用于在连接区域连接包含样品的介质到样品分离介质，所述样品包含含有样品的介质和满足特定等式的连接区域。 由此，可以提供比现有技术更好的精度的电泳技术。

公开(公告)号：US08500981B2

公开(公告)日：2013-08-06

申请号：US13054271

申请日：2009-07-15

申请人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

发明人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44773

摘要： An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.

摘要翻译： 电泳装置包括样品分离部分，用于容纳用于在水平方向上分离样品的样品分离介质，样品分离部分包含样品分离介质，使得样品分离介质具有暴露部分，至少一端 所述样品分离介质的表面与所述水平方向平行; 以及介质连接装置，用于在连接区域连接包含样品的介质到样品分离介质，所述样品包含含有样品的介质和满足特定等式的连接区域。 由此，可以提供比现有技术更好的精度的电泳技术。

公开(公告)号：US20080296158A1

公开(公告)日：2008-12-04

申请号：US12130691

申请日：2008-05-30

申请人： Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

发明人： Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

IPC分类号： C25B7/00 ,  C25B9/00

CPC分类号： G01N27/44704 ,  B01L3/50273

摘要： A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.

摘要翻译： 用于电泳的装置将电压施加到与多个电导体接触的介质，使得相邻导体的电位在一定范围内。 这样可以防止电泳速度下降。 用于电泳和转印的装置包括具有彼此绝缘并沿特定方向布置的多个电极区域的电极。 这允许提供用于电泳和转移的实用且易于使用的装置。 用于传送的装置将施加的电压或施加的电压持续时间改变到某个位置到另一位置。 这样可以提高传输效率。

公开(公告)号：US08702950B2

公开(公告)日：2014-04-22

申请号：US13607844

申请日：2012-09-10

申请人： Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

发明人： Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

IPC分类号： G01N27/26 ,  B01D57/02

CPC分类号： G01N27/44704 ,  B01L3/50273

摘要： A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.

摘要翻译： 用于电泳的装置将电压施加到与多个电导体接触的介质，使得相邻导体的电位在一定范围内。 这样可以防止电泳速度下降。 用于电泳和转印的装置包括具有彼此绝缘并沿特定方向布置的多个电极区域的电极。 这允许提供用于电泳和转移的实用且易于使用的装置。 用于传送的装置将施加的电压或施加的电压持续时间改变到某个位置到另一位置。 这样可以提高传输效率。

公开(公告)号：US20130015069A1

公开(公告)日：2013-01-17

申请号：US13607844

申请日：2012-09-10

申请人： Yuji MARUO ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

发明人： Yuji MARUO ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

IPC分类号： B01D57/02

CPC分类号： G01N27/44704 ,  B01L3/50273

摘要： A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.

摘要翻译： 用于电泳的装置将电压施加到与多个电导体接触的介质，使得相邻导体的电位在一定范围内。 这样可以防止电泳速度下降。 用于电泳和转印的装置包括具有彼此绝缘并沿特定方向布置的多个电极区域的电极。 这允许提供用于电泳和转移的实用且易于使用的装置。 用于传送的装置将施加的电压或施加的电压持续时间改变到某个位置到另一位置。 这样可以提高传输效率。

公开(公告)号：US20120273360A1

公开(公告)日：2012-11-01

申请号：US13518686

申请日：2010-12-21

申请人： Hideki Kinoshita ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Koji Sakairi ,  Satonari Akutsu

发明人： Hideki Kinoshita ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Koji Sakairi ,  Satonari Akutsu

IPC分类号： C25B9/00 ,  G01N27/447

CPC分类号： G01N27/44756 ,  G01N27/44743

摘要： An instrument for electrophoresis includes a sample-separating portion in which a slab sample-separating medium that separates a sample is provided. The instrument for electrophoresis includes a sample-transporting portion that transports a sample-containing medium containing the sample to the sample-separating portion. The instrument for electrophoresis includes a positioning portion that is provided to the sample-separating portion and/or the sample-transporting portion and used for connecting the sample-transporting portion to a predetermined position of the sample-separating portion.

摘要翻译： 电子仪器包括样品分离部分，其中提供分离样品的板坯样品分离介质。 用于电泳的仪器包括将含有样品的含样本培养基输送到样品分离部分的样品输送部分。 电泳仪包括设置在样品分离部分和/或样品输送部分上的定位部分，用于将样品输送部分连接到样品分离部分的预定位置。

公开(公告)号：US20090084681A1

公开(公告)日：2009-04-02

申请号：US12237816

申请日：2008-09-25

申请人： Satonari AKUTSU ,  Koji Sakairi ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama

发明人： Satonari AKUTSU ,  Koji Sakairi ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama

IPC分类号： B01D61/42 ,  B05D5/12

CPC分类号： G01N27/44747

摘要： With the use of a chip (4) for electrophoresis and transfer including a transfer membrane (1) and an electrophoretic gel that is integrated with the transfer membrane (1), separation of a sample by electrophoresis and transfer of the sample separated to the transfer membrane (1) are carried out consecutively. This allows molecules separated on the gel by electrophoresis to be accurately transferred to the transfer membrane.

摘要翻译： 通过使用芯片（4）进行电泳和转印，包括转印膜（1）和与转印膜（1）整合的电泳凝胶，通过电泳分离样品并将样品分离转移到转印 膜（1）连续进行。 这允许通过电泳在凝胶上分离的分子被精确地转移到转移膜上。

公开(公告)号：US08449748B2

公开(公告)日：2013-05-28

申请号：US13001274

申请日：2009-06-01

申请人： Uichi Midorikawa ,  Takateru Matsunaga ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama

发明人： Uichi Midorikawa ,  Takateru Matsunaga ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama

IPC分类号： G01N27/447

CPC分类号： G01N27/4473

摘要： A sample separation/adsorption appliance (100) according to one embodiment of the present invention includes: a first buffer solution tank (103) equipped with a first electrode (101) and used for holding a first buffer solution; a second buffer solution tank (104) equipped with a second electrode (102) and used for holding a second buffer solution; a sample separation section (110) that holds a sample separation medium (131) for separating a sample; and a conductive section (120) that holds a conductive medium (133), wherein: the sample separation medium (131) and the conductive medium (133) are in contact with the sample adsorption member (132), respectively, on the opposite sides thereof; an end of the sample separation medium (131) opposite to an end being in contact with the sample adsorption member (132) is connected to an inside of the first buffer solution tank (103); and an end of the conductive medium (133) opposite to an end being in contact with the sample adsorption member (132) is connected to an inside of the second buffer solution tank (104). With this arrangement, a sample separation/adsorption appliance that enables efficient transfer of a sample is offered.

摘要翻译： 根据本发明的一个实施方案的样品分离/吸附装置（100）包括：装备有第一电极（101）并用于保持第一缓冲溶液的第一缓冲溶液罐（103） 装备有第二电极（102）并用于保持第二缓冲溶液的第二缓冲溶液罐（104）; 样品分离部（110），其保持用于分离样品的样品分离介质（131） 以及保持导电介质（133）的导电部（120），其中：所述样品分离介质（131）和所述导电介质（133）分别在所述样品吸附部件（132）的相对侧 的; 与第一缓冲溶液罐（103）的内部连接的是与样品吸附部件（132）接触的端部相反的样品分离介质（131）的端部。 并且与与样品吸附构件（132）接触的端部相对的导电介质（133）的端部连接到第二缓冲溶液罐（104）的内部。 通过这种布置，提供了能够有效地转移样品的样品分离/吸附装置。