公开(公告)号：US06562954B1

公开(公告)日：2003-05-13

申请号：US09478025

申请日：2000-01-05

申请人： Yuji Matsuzaki ,  Yousuke Yasuda ,  Satoshi Miyauchi ,  Junichi Onaya ,  Yusuke Hori ,  Akira Tawada ,  Hideo Mochizuki ,  Masami Iida

发明人： Yuji Matsuzaki ,  Yousuke Yasuda ,  Satoshi Miyauchi ,  Junichi Onaya ,  Yusuke Hori ,  Akira Tawada ,  Hideo Mochizuki ,  Masami Iida

IPC分类号： C07H1702

CPC分类号： C07H11/00 ,  Y02P20/55

摘要： Oligosaccharides including those usable as anti-inflammatory agent or anti-allergy agent, represented by the following general formula (3): wherein R10 and R11 each independently represent a hydrogen atom or —SO3M where M represents a proton or a monovalent cation, Ac represents an acetyl group, R12 represents a hydrogen atom or an anomeric substituent (a 6-O-sulfated N-acetylglucosamine residue, an alkyl group, a glycerol residue, an O-alkylglycerol residue, a cholesterol residue, a cholestanyl group, a ceramide residue, a phospholipid residue, a biotin residue, or a peptide residue), and Z represents an oxygen atom or —NHCO—.

摘要翻译： 包括可用作抗炎剂或抗过敏剂的寡糖，由以下通式（3）表示：其中R10和R11各自独立地表示氢原子或-SO3M，其中M表示质子或一价阳离子，Ac表示 乙酰基，R 12表示氢原子或端基异构取代基（6-O-硫酸化N-乙酰葡糖胺残基，烷基，甘油残基，O-烷基甘油残基，胆固醇残基，胆甾烷基，神经酰胺残基 ，磷脂残基，生物素残基或肽残基），Z表示氧原子或-NHCO-。

公开(公告)号：US06492503B1

公开(公告)日：2002-12-10

申请号：US09509478

申请日：2000-05-22

申请人： Yutaka Kariya ,  Ryo Takano ,  Kaeko Kamei ,  Saburo Hara ,  Junichi Onaya ,  Yusuke Hori

发明人： Yutaka Kariya ,  Ryo Takano ,  Kaeko Kamei ,  Saburo Hara ,  Junichi Onaya ,  Yusuke Hori

IPC分类号： C07H100

CPC分类号： C08B37/0075 ,  C08B37/0063 ,  Y10T436/143333

摘要： There is provided a glycosaminoglycan having a backbone structure comprising a repetitive disaccharide bearing a uronic acid residue and a glucosamine residue, and having sulfate groups, wherein substantially no sulfate group bound to the hydroxyl group at the 6-position of the glucosamine residue in the backbone structure is detected as determined by a chemical disaccharide analysis method in which the glycosaminoglycan is decomposed with nitrous acid, reacted with para-nitrophenylhydrazine and analyzed by high performance liquid chromatography, and the molar % of a uronic acid residue having a sulfate group at the 2-position is not less than 45% relative to total uronic acid residues, which is calculated from a disaccharide composition obtained by an enzymatic disaccharide analysis method in which the glycosaminoglycan is digested with glycosaminoglycan-degrading enzymes and analyzed by high performance liquid chromatography. The glycosaminoglycan can be used as an active ingredient of pharmaceuticals.

摘要翻译： 提供了具有骨架结构的糖胺聚糖，该骨架结构包含具有糖醛酸残基和葡糖胺残基的重复二糖并且具有硫酸基，其中基本上没有与骨架中的葡糖胺残基的6-位上的羟基结合的硫酸基 通过化学二糖分析方法检测结构，其中糖胺聚糖用亚硝酸分解，与对硝基苯肼反应并通过高效液相色谱法分析，并且具有硫酸根的糖醛酸残基的摩尔％在2 - 相对于总糖醛酸残基不低于45％，其由通过其中糖胺聚糖用糖胺聚糖降解酶消化并通过高效液相色谱分析的酶二糖分析方法获得的二糖组合物计算。 糖胺聚糖可以用作药物的活性成分。

公开(公告)号：US5801162A

公开(公告)日：1998-09-01

申请号：US446662

申请日：1995-08-28

申请人： Akikazu Takada ,  Junichi Onaya ,  Mikio Arai ,  Satoshi Miyauchi ,  Mamoru Kyogashima ,  Keiichi Yoshida

发明人： Akikazu Takada ,  Junichi Onaya ,  Mikio Arai ,  Satoshi Miyauchi ,  Mamoru Kyogashima ,  Keiichi Yoshida

IPC分类号： A61K31/715 ,  A61K31/726 ,  A61K31/737 ,  A61K38/36 ,  A61K38/49 ,  A61P7/02 ,  A61P9/10 ,  C08B37/00 ,  C08B37/08 ,  C08L5/00 ,  C08L5/08

CPC分类号： A61K38/49 ,  A61K31/715 ,  A61K31/726 ,  A61K31/737 ,  C08B37/0069 ,  C08L5/00 ,  C08L5/08

摘要： Dermatan sulfate compositions having certain characteristics have been found to have thrombolytic activity. Dermatan sulfate combined with tissue plasminogen activator (t-PA) enhances the thrombolytic activity of t-PA. Antithrombotic compositions containing dermatan sulfates can be used for treating various thrombotic diseases.

摘要翻译： PCT No.PCT / JP94 / 01643 Sec。 371日期：1995年8月28日 102（e）日期1995年8月28日PCT 1994年9月30日PCT公布。 公开号WO95 / 09188 日期1995年4月6日已经发现具有某些特征的硫酸皮肤素组合物具有溶栓活性。 硫酸皮肤素组合纤维蛋白溶酶原激活剂（t-PA）增强了t-PA的溶栓活性。 含有硫酸皮肤素的抗血栓组合物可用于治疗各种血栓性疾病。

公开(公告)号：US20110318759A1

公开(公告)日：2011-12-29

申请号：US13142262

申请日：2009-12-24

申请人： Shigeyuki Wakitani ,  Hiroshi Fujita ,  Takeshi Ishimaru ,  Koji Yamamoto ,  Yasuhiro Kurahashi ,  Junichi Onaya ,  Hiroyuki Masuda

发明人： Shigeyuki Wakitani ,  Hiroshi Fujita ,  Takeshi Ishimaru ,  Koji Yamamoto ,  Yasuhiro Kurahashi ,  Junichi Onaya ,  Hiroyuki Masuda

IPC分类号： G01N33/577 ,  C07K16/18

CPC分类号： G01N33/53 ,  G01N2400/40 ,  G01N2800/102

摘要： The inventions provides a method for immunologically determining a keratan sulfate level which method includes bringing an anti-keratan sulfate monoclonal antibody into contact with a biological sample, the anti-keratan sulfate monoclonal antibody exhibiting a relative reaction specificity between keratan sulfate-I and keratan sulfate-II represented by IC50KS-I/KS-II of 0.4 to 5, to thereby provide a signal; and detecting keratan sulfate contained in the biological sample from the signal. On the basis of the method, the invention also provides a joint disease detection method and a method for assessing the effect of a remedy for a joint disease and a candidate substance therefor. Through these methods, a very small amount of keratan sulfate contained in a sample, can be determined. Particularly, these methods can determine, at high-sensitivity and high-specificity, the total keratan sulfate including keratan sulfate-I, which have been difficult to determine through a conventional technique. The methods also enables detect a joint disease and assess the effect of a remedy for a joint disease or a candidate substance therefor.

摘要翻译： 本发明提供了一种免疫测定硫酸角质素水平的方法，该方法包括使硫酸角质素硫酸单克隆抗体与生物样品接触，硫酸角质素-I与硫酸角质素之间呈现相对反应特异性的硫酸角质素单体抗体 -II由IC50KS-I / KS-II表示，为0.4〜5，从而提供信号; 并从信号中检测生物样品中所含的硫酸角质素。 在该方法的基础上，本发明还提供了关于联合疾病的检测方法和评估对于关节疾病及其候选物质的补救效果的方法。 通过这些方法，可以测定样品中所含的极少量硫酸角质素。 特别地，这些方法可以高灵敏度和高特异性地确定通过常规技术难以确定的总硫酸角质素，包括硫酸角质素-I。 该方法还能够检测关节疾病并评估对于关节疾病或候选物质的补救的效果。

公开(公告)号：US08822229B2

公开(公告)日：2014-09-02

申请号：US13142262

申请日：2009-12-24

申请人： Shigeyuki Wakitani ,  Hiroshi Fujita ,  Takeshi Ishimaru ,  Koji Yamamoto ,  Yasuhiro Kurahashi ,  Junichi Onaya ,  Hiroyuki Masuda

发明人： Shigeyuki Wakitani ,  Hiroshi Fujita ,  Takeshi Ishimaru ,  Koji Yamamoto ,  Yasuhiro Kurahashi ,  Junichi Onaya ,  Hiroyuki Masuda

IPC分类号： G01N33/564

CPC分类号： G01N33/53 ,  G01N2400/40 ,  G01N2800/102

摘要： The inventions provides a method for immunologically determining a keratan sulfate level which method includes bringing an anti-keratan sulfate monoclonal antibody into contact with a biological sample, the anti-keratan sulfate monoclonal antibody exhibiting a relative reaction specificity between keratan sulfate-I and keratan sulfate-II represented by IC50KS-I/KS-II of 0.4 to 5, to thereby provide a signal; and detecting keratan sulfate contained in the biological sample from the signal. On the basis of the method, the invention also provides a joint disease detection method and a method for assessing the effect of a remedy for a joint disease and a candidate substance therefor. Through these methods, a very small amount of keratan sulfate contained in a sample, can be determined. Particularly, these methods can determine, at high-sensitivity and high-specificity, the total keratan sulfate including keratan sulfate-I, which have been difficult to determine through a conventional technique. The methods also enables detect a joint disease and assess the effect of a remedy for a joint disease or a candidate substance therefor.

摘要翻译： 本发明提供了一种免疫测定硫酸角质素水平的方法，该方法包括使硫酸角质素硫酸单克隆抗体与生物样品接触，硫酸角质素-I与硫酸角质素之间呈现相对反应特异性的硫酸角质素单体抗体 -II由IC50KS-I / KS-II表示，为0.4〜5，从而提供信号; 并从信号中检测生物样品中所含的硫酸角质素。 在该方法的基础上，本发明还提供了关于联合疾病的检测方法和评估对于关节疾病及其候选物质的补救效果的方法。 通过这些方法，可以测定样品中所含的极少量硫酸角质素。 特别地，这些方法可以高灵敏度和高特异性地确定通过常规技术难以确定的总硫酸角质素，包括硫酸角质素-I。 该方法还能够检测关节疾病并评估对于关节疾病或候选物质的补救的效果。

公开(公告)号：US20060105991A1

公开(公告)日：2006-05-18

申请号：US11324407

申请日：2006-01-04

申请人： Shin-ichi Ishikawa ,  Junichi Onaya

发明人： Shin-ichi Ishikawa ,  Junichi Onaya

IPC分类号： A61K31/728

CPC分类号： A61K31/728 ,  A61K31/726

摘要： A medical composition for protuberance of epithelium, which comprises a solution comprising a polysaccharide or a medically acceptable salt thereof, wherein the solution has a viscosity of: (1) from 50 to 500 mPa·s at a shear rate of from 7.7 to 10.0 s−1; (2) from 45 to 300 mPa·s at a shear rate of from 19.2 to 20.0 s−1; and (3) from 40 to 200 mPa·s at a shear rate of from 38.3 to 40.0 s−1, when measured using a rotational viscometer at 25° C., and a syringe filled with the medical composition.

公开(公告)号：US07008626B2

公开(公告)日：2006-03-07

申请号：US10286822

申请日：2002-11-04

申请人： Shin-ichi Ishikawa ,  Junichi Onaya

发明人： Shin-ichi Ishikawa ,  Junichi Onaya

IPC分类号： A61K7/00

CPC分类号： A61K31/728 ,  A61K31/726

摘要： A medical composition for protuberance of epithelium, which comprises a solution comprising a polysaccharide or a medically acceptable salt thereof, wherein the solution has a viscosity of: (1) from 50 to 500 mPa·s at a shear rate of from 7.7 to 10.0 s−1; (2) from 45 to 300 mPa·s at a shear rate of from 19.2 to 20.0 s−1; and (3) from 40 to 200 mPa·s at a shear rate of from 38.3 to 40.0 s−1, when measured using a rotational viscometer at 25° C., and a syringe filled with the medical composition.

摘要翻译： 一种用于上皮隆起的药物组合物，其包含含有多糖或其医学上可接受的盐的溶液，其中所述溶液的粘度为：（1）在7.7至10.0s的剪切速率下为50至500mPa.s ^{-1 ; （2）在剪切速率为19.2至20.0s -1的范围内为45至300mPa.s; 和（3）当在25℃下使用旋转粘度计测量时，剪切速率为38.3至40.0s -1的剪切速率为40至200mPa.s，以及填充有医疗的注射器 组成。}