-
公开(公告)号：US20140080143A1
公开(公告)日：2014-03-20
申请号：US14008441
申请日：2012-03-28
IPC分类号： G01N33/573
CPC分类号： G01N33/543 , G01N33/573 , G01N33/57434 , G01N33/577 , G01N2333/96433
摘要： Provided is a method for the simple and highly accurate assay of PSA by a one-step reaction that does not use carriers having different average grain sizes. Also provided is a reagent therefor. The PSA assay method comprises sensitizing insoluble carriers having the same average grain size within a range of greater than 0.20 μm but 0.40 μm or less using two types of anti-PSA monoclonal antibodies having different epitopes that are anti-PSA monoclonal antibodies that will react with both free PSA and PSA-ACT, which is a complex of free PSA and α1-antichymotrypsin, and bringing the carriers into contact with a sample in the presence of an aggregation promoter.
摘要翻译： 提供了通过不使用具有不同平均粒径的载体的一步反应简单且高度精确地测定PSA的方法。 还提供了一种试剂。 PSA测定方法包括使用具有不同表位的两种类型的抗PSA单克隆抗体对具有相同平均粒径的不溶性载体敏感，所述抗原单克隆抗体是抗PSA单克隆抗体，其将与 游离PSA和PSA-ACT两者均为游离PSA和α1-抗胰凝乳蛋白酶的复合物，并使载体在聚集促进剂存在下与样品接触。
-
公开(公告)号：US20110236996A1
公开(公告)日：2011-09-29
申请号：US13132790
申请日：2009-12-03
IPC分类号： G01N33/68
CPC分类号： G01N33/543 , G01N33/6893 , G01N2333/8139
摘要： There is provided a particle-enhanced immunoassay method for cystatin C in a human body fluid, which has higher specificity and is easily automatable at low cost as compared with conventional assay methods that use large amounts of polyclonal antibodies having low specificity or monoclonal antibodies having high specificity but having poor agglutinability.Disclosed is an assay method specific to cystatin C in a human body fluid, which is a particle-enhanced immunoassay method utilizes a combination of antibody-coated particles in which some insoluble carrier particles are coated with anti-human cystatin C monoclonal antibodies of one type which have high affinity and in which some other insoluble carrier particles are coated with anti-human cystatin C monoclonal antibodies of one type which recognize an epitope different from that of the monoclonal antibody of the first-mentioned type and which have relatively low affinity, wherein the binding amount of the anti-human cystatin C monoclonal antibodies relative to the total weight of the particles being coated with the corresponding antibodies is less than 5% by weight.
摘要翻译： 与使用大量具有低特异性的多克隆抗体或具有高特异性的单克隆抗体的常规测定方法相比，提供了人体液中半胱氨酸蛋白酶抑制剂C的微粒增强免疫测定方法，其具有更高的特异性并且可以以低成本容易地自动化 特异性但具有差的凝集性。 本发明公开了一种人体液中半胱氨酸蛋白酶抑制剂C特异性检测方法，其是使用抗体包被的颗粒的组合，其中一些不溶性载体颗粒被一种类型的抗人半胱氨酸蛋白酶抑制剂C单克隆抗体包被， 其具有高亲和力，并且其中一些其它不溶性载体颗粒被一种类型的抗人半胱氨酸蛋白酶抑制剂C单克隆抗体包被，所述抗人半胱氨酸蛋白酶抑制剂C单克隆抗体识别与第一类型的单克隆抗体的表位不同并具有相对低亲和力的表位，其中 抗人半胱氨酸蛋白酶抑制剂C单克隆抗体相对于被相应抗体包被的颗粒的总重量的结合量小于5重量％。
-
公开(公告)号：US08802446B2
公开(公告)日：2014-08-12
申请号：US13132790
申请日：2009-12-03
IPC分类号： G01N33/543
CPC分类号： G01N33/543 , G01N33/6893 , G01N2333/8139
摘要： Provided is a method and reagent for measuring cystatin C in a human body fluid, by contacting a human body fluid with (a) first insoluble carrier particles coated with a first anti-human cystatin C monoclonal antibody and (b) second insoluble carrier particles coated with a second anti-human cystatin C monoclonal antibody, where the first anti-human cystatin C monoclonal antibody has an affinity for cystatin C that is higher than the second anti-human cystatin C monoclonal antibody, the second anti-human cystatin C monoclonal antibody recognizes an epitope of cystatin C that is different from the epitope recognized by the first anti-human cystatin C monoclonal antibody, and the first and second insoluble carrier particles are coated with from 1% to less than 4% by weight of the anti-human cystatin C monoclonal antibodies.
摘要翻译： 本发明提供一种通过使人体液与（a）涂覆有第一抗人半胱氨酸蛋白酶抑制剂C单克隆抗体的第一不溶性载体颗粒和（b）涂覆有第一抗人半胱氨酸蛋白酶抑制剂C单克隆抗体的第二不溶性载体颗粒接触来测量人体液体中的半胱氨酸蛋白酶抑制剂C的方法和试剂 使用第二抗人半胱氨酸蛋白酶抑制剂C单克隆抗体，其中第一抗人半胱氨酸蛋白酶抑制剂C单克隆抗体对半胱氨酸蛋白酶抑制剂C具有高于第二抗人半胱氨酸蛋白酶抑制剂C单克隆抗体的亲和力，第二抗人半胱氨酸蛋白酶抑制剂C单克隆抗体 识别与由第一抗人半胱氨酸蛋白酶抑制剂C单克隆抗体识别的表位不同的半胱氨酸蛋白酶抑制剂C的表位，并且第一和第二不溶性载体颗粒涂覆有1％至小于4重量％的抗人 半胱氨酸蛋白酶抑制剂C单克隆抗体。
-
公开(公告)号：US20110165701A1
公开(公告)日：2011-07-07
申请号：US13061849
申请日：2009-09-03
CPC分类号： G01N33/545 , C07K16/4283 , G01N33/541 , G01N33/54393 , G01N33/6854
摘要： An objective of the present invention is to provide an anti-human IgM monoclonal antibody that is capable of reacting specifically with human IgM and inducing immunoagglutination based on an antigen-antibody reaction with human IgM in solution, and an immunoassay using the said monoclonal antibody. Another objective of the present invention is to provide an agent for suppressing non-specific reactions caused by human IgM that could not be prevented by conventional methods, and an immunoassay in which non-specific reactions caused by human IgM are suppressed.By selecting a monoclonal antibody that reacts with human IgM on the basis of evaluation of reactivity with human IgM in solution, a novel monoclonal antibody capable of agglutinating human IgM by itself and performing a practical immunoagglutination assay has been obtained, and the objectives above have been thus achieved.
摘要翻译： 本发明的目的是提供能够与人IgM特异性反应并基于与溶液中的人IgM的抗原抗体反应诱导免疫凝集的抗人IgM单克隆抗体，以及使用所述单克隆抗体的免疫测定。 本发明的另一个目的是提供一种用于抑制由人IgM引起的非特异性反应的试剂，其不能通过常规方法进行抑制，并且抑制由人IgM引起的非特异性反应的免疫测定。 通过选择基于与溶液中的人IgM的反应性的评价与人IgM反应的单克隆抗体，获得能够自身凝集人IgM并进行实际的免疫凝集试验的新型单克隆抗体，并且已经得到上述目的 从而实现。
-
公开(公告)号：US20130004976A1
公开(公告)日：2013-01-03
申请号：US13518788
申请日：2010-12-27
IPC分类号： G01N33/566 , G01N30/00 , C07K16/26 , G01N33/577
CPC分类号： G01N33/74 , C07K16/26 , C07K2317/33 , C07K2317/34 , G01N2333/62
摘要： A problem of the present invention is to provide an antibody specific to human insulin and an assay and an assay reagent using the antibody capable of accurately assaying human insulin without being affected by porcine insulin. The present invention provides an assay and an assay reagent capable of specifically assaying human insulin by combining a monoclonal antibody specifically reactive with human insulin and nonreactive with porcine insulin and a different anti-human insulin antibody.
摘要翻译： 本发明的一个问题是提供一种对人胰岛素有特异性的抗体以及使用能够精确测定人胰岛素而不受猪胰岛素影响的抗体的测定法和测定试剂。 本发明提供能够通过将与胰岛素特异性反应的单克隆抗体与猪胰岛素和不同抗人胰岛素抗体反应的单克隆抗体结合来特异性测定人胰岛素的测定法和测定试剂。
-
公开(公告)号：US20110195438A1
公开(公告)日：2011-08-11
申请号：US13122930
申请日：2010-07-21
CPC分类号： G01N33/74 , C07K16/26 , G01N33/53 , G01N2333/62
摘要： The present invention provides an insulin-specific assay and an assay reagent capable of sensitively and specifically assaying insulin using an antibody having a property of reacting with insulin bound to an anti-insulin antibody while not reacting with insulin not bound to an anti-insulin antibody, without being affected by proinsulin and insulin analogs.
摘要翻译： 本发明提供一种胰岛素特异性测定法和能够使用具有与结合抗胰岛素抗体结合的胰岛素反应的性质而不与未与抗胰岛素抗体结合的胰岛素反应的抗体敏感且特异性地测定胰岛素的测定试剂 ，而不受胰岛素原和胰岛素类似物的影响。
-
公开(公告)号：US09476891B2
公开(公告)日：2016-10-25
申请号：US13122930
申请日：2010-07-21
CPC分类号： G01N33/74 , C07K16/26 , G01N33/53 , G01N2333/62
摘要： The present invention provides an insulin-specific assay and an assay reagent capable of sensitively and specifically assaying insulin using an antibody having a property of reacting with insulin bound to an anti-insulin antibody while not reacting with insulin not bound to an anti-insulin antibody, without being affected by proinsulin and insulin analogs.
摘要翻译： 本发明提供一种胰岛素特异性测定法和能够使用具有与结合抗胰岛素抗体结合的胰岛素反应的性质而不与未与抗胰岛素抗体结合的胰岛素反应的抗体敏感且特异性地测定胰岛素的测定试剂 ，而不受胰岛素原和胰岛素类似物的影响。
-
公开(公告)号：US20150301038A1
公开(公告)日：2015-10-22
申请号：US14129771
申请日：2012-06-29
申请人： Shinya Yokokawa , Mitsuaki Yamamoto , Tomo Shimizu
发明人： Shinya Yokokawa , Mitsuaki Yamamoto , Tomo Shimizu
IPC分类号： G01N33/553 , C07K17/14 , C07K17/08 , C07K16/36 , C07K14/765
CPC分类号： G01N33/553 , C07K14/765 , C07K16/36 , C07K17/08 , C07K17/14
摘要： To adjust detection sensitivity and expand a measurement range depending on objects to be detected by a simple procedure in an immunoassay method involving using a colloidal metal having immobilized thereon an antibody or an antigen. Blocking treatment for a surface of a colloidal metal particle having immobilized thereon an antibody or an antigen with both components, i.e., a component of biological origin and a component of non-biological origin enables adjustment of measurement sensitivity with, for example, kinds and concentrations of both the components, and enables use of the component of non-biological origin, which has not been usable alone because of aggregation of a colloidal metal.
摘要翻译： 为了调整检测灵敏度，根据涉及使用固定有抗体或抗原的胶体金属的免疫测定方法，通过简单的步骤检测的对象扩大测定范围。 在其上固定有抗体或具有两种成分的成分，即生物来源的成分和非生物来源的成分的胶体金属颗粒的表面的封闭处理使得能够例如通过种类和浓度来调节测量灵敏度 并且能够使用由于胶体金属的聚集而单独使用的非生物来源的成分。
-
公开(公告)号：US06433412B1
公开(公告)日：2002-08-13
申请号：US09800589
申请日：2001-03-08
申请人： Hideko Ando , Hiroshi Kikuchi , Ikuo Yoshida , Toshihiko Sato , Tomo Shimizu
发明人： Hideko Ando , Hiroshi Kikuchi , Ikuo Yoshida , Toshihiko Sato , Tomo Shimizu
IPC分类号： H01L2302
摘要： A central portion of a main face of a package substrate 2 is mounted with a memory chip 1 using face down bonding by a flip chip bonding system. Further, a plurality of chip condensers 7 are mounted at vicinities of the memory chip 1. A clearance between a main face (lower face) of the memory chip 1 and a main face of the package substrate 2 is filled with underfill resin (seal resin) 10 constituting a seal member for achieving protection of connecting portions and for relaxation of thermal stress. An outer edge of the underfill resin 10 is extended to an outer side of the memory chip 1 and covers entire faces of the chip condensers 7 mounted at vicinities of the memory chip 1.
-
-
-
-
-
-
-
-
搜索结果
9 条记录 (耗时 0.023 秒)
