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公开(公告)号：US07105719B1
公开(公告)日：2006-09-12
申请号：US08894356
申请日：1996-02-16
申请人： Toshihiko Ashikari , Yoshikazu Tanaka , Hiroyuki Fujiwara , Masahiro Nakao , Yuko Fukui , Keiko Sakakibara , Masako Mizutani , Takaaki Kusumi
发明人： Toshihiko Ashikari , Yoshikazu Tanaka , Hiroyuki Fujiwara , Masahiro Nakao , Yuko Fukui , Keiko Sakakibara , Masako Mizutani , Takaaki Kusumi
CPC分类号： C07H15/203 , C12N9/1029 , C12N15/825 , C12P19/445
摘要： The invention relates to isolated polynucleotides encoding a protein having aromatic acyltransferase activity, vectors comprising aromatic acyltransferase polynucleotides, and methods of transforming plants with anthocyanin acyltransferase polynucleotides to alter flower colors. The invention also relates to transformed host cells, plants, and flower cuts expressing proteins having anthocyanin acyltransferase activity.
摘要翻译： 本发明涉及编码具有芳族酰基转移酶活性的蛋白质的分离的多核苷酸，包含芳族酰基转移酶多核苷酸的载体，以及用花青素酰基转移酶多核苷酸转化植物以改变花色的方法。 本发明还涉及表达具有花青素酰基转移酶活性的蛋白质的转化的宿主细胞，植物和花切片。
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公开(公告)号：US20060020123A1
公开(公告)日：2006-01-26
申请号：US11207721
申请日：2005-08-22
申请人： Keiko Sakakibara , Yuko Fukui , Yoshikazu Tanaka , Takaaki Kusumi , Masako Mizutani , Toru Nakayama
发明人： Keiko Sakakibara , Yuko Fukui , Yoshikazu Tanaka , Takaaki Kusumi , Masako Mizutani , Toru Nakayama
CPC分类号： C12N9/0059 , C12N15/52 , C12N15/825 , C12P17/04
摘要： The present invention provides a protein having aurone synthase activity involved in the color of flowers such as snapdragons, a gene that encodes it, cDNA in particular, and its applications. This gene can give yellow color to the flowers of plants by introducing into plants deficient in chalcone isomerase and so forth and expressing.
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公开(公告)号：US06982325B1
公开(公告)日：2006-01-03
申请号：US09446089
申请日：1999-04-16
申请人： Keiko Sakakibara , Yuko Fukui , Yoshikazu Tanaka , Takaaki Kusumi , Masako Mizutani , Toru Nakayama
发明人： Keiko Sakakibara , Yuko Fukui , Yoshikazu Tanaka , Takaaki Kusumi , Masako Mizutani , Toru Nakayama
IPC分类号： C07H21/04
CPC分类号： C12N9/0059 , C12N15/52 , C12N15/825 , C12P17/04
摘要： The present invention provides a protein having aurone synthase activity involved in the color of flowers such as snapdragons, a gene that encodes it, cDNA in particular, and its applications. This gene can give yellow color to the flowers of plants by introducing into plants deficient in chalcone isomerase and so forth and expressing.
摘要翻译： 本发明提供一种具有参与花的颜色的鸢尾醇合成酶活性的蛋白质，例如快速克隆，编码它的基因，特别是cDNA及其应用。 该基因可以通过引入缺乏查耳酮异构酶等的植物并进行表达而赋予植物花黄色。
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公开(公告)号：US07592436B2
公开(公告)日：2009-09-22
申请号：US11207721
申请日：2005-08-22
申请人： Keiko Sakakibara , Yuko Fukui , Yoshikazu Tanaka , Takaaki Kusumi , Masako Mizutani , Toru Nakayama
发明人： Keiko Sakakibara , Yuko Fukui , Yoshikazu Tanaka , Takaaki Kusumi , Masako Mizutani , Toru Nakayama
CPC分类号： C12N9/0059 , C12N15/52 , C12N15/825 , C12P17/04
摘要： The present invention provides a protein having aurone synthase activity involved in the color of flowers such as snapdragons, a gene that encodes it, cDNA in particular, and its applications. This gene can give yellow color to the flowers of plants by introducing into plants deficient in chalcone isomerase and so forth and expressing.
摘要翻译： 本发明提供一种具有参与花的颜色的鸢尾醇合成酶活性的蛋白质，例如快速克隆，编码它的基因，特别是cDNA及其应用。 该基因可以通过引入缺乏查耳酮异构酶等的植物并进行表达而赋予植物花黄色。
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公开(公告)号：US07160700B2
公开(公告)日：2007-01-09
申请号：US10048866
申请日：2001-06-01
CPC分类号： C12N15/825 , C12N9/1048 , C12N9/1051
摘要： A nucleic acid encoding a protein which has an amino acid sequence according to SEQ ID NO: 2, 12, or 14, or the amino acid sequence modified by addition or deletion of one or more amino acids and/or substitution by other amino acids, and which has activity to transfer a glycosyl group to the 3′-position of anthocyanins.
摘要翻译： 编码具有根据SEQ ID NO：2,12或14的氨基酸序列的蛋白质的核酸或通过添加或缺失一个或多个氨基酸和/或被其它氨基酸取代而修饰的氨基酸序列， 并且具有将糖基转移到花青素的3'-位的活性。
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公开(公告)号：US20050003476A1
公开(公告)日：2005-01-06
申请号：US10048866
申请日：2001-06-01
IPC分类号： C12N1/15 , C12N1/19 , C12N1/21 , C12N9/10 , C12N15/29 , C12N15/54 , C12N15/82 , A01H1/00 , C07H21/04 , C12N5/04 , C12P19/60
CPC分类号： C12N15/825 , C12N9/1048 , C12N9/1051
摘要： A gene encoding a protein which has an amino acid sequence according to SEQ ID NO: 2, 12, or 14, or the amino acid sequence modified by addition or deletion of one or more amino acids and/or substitution by other amino acids, and which has activity to transfer a glycosyl group to the 3′-position of anthocyanins.
摘要翻译： 编码具有根据SEQ ID NO：2,12或14的氨基酸序列的蛋白质的基因或通过添加或缺失一个或多个氨基酸和/或被其它氨基酸取代而修饰的氨基酸序列，以及 其具有将糖基转移到花青素的3'-位的活性。
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7.发明授权Genes encoding protein having activity of transferring sugar onto aurone 失效编码蛋白质的基因，其具有将糖转移到噢one having上的活性公开(公告)号：US06770747B1
公开(公告)日：2004-08-03
申请号：US09673300
申请日：2000-10-16
IPC分类号： C07H2104
CPC分类号： C12N15/825 , C12N9/1048 , C12N9/1051 , C12N15/8243 , C12P19/60
摘要： There is provided a gene encoding a protein derived from, for example, snapdragons and petunias, said protein having an amino acid sequence as set forth in SEQ ID NO: 2, 8, and 10, and having an activity of transferring a glycosyl group to aurones, and a method of producing said protein using said gene. By introducing this gene into plants that do not have said gene, a yellow pigment aurone can be stabilized and plants having yellow flowers can be obtained.
摘要翻译： 提供了编码来自例如快速聚糖和矮牵牛的蛋白质的基因，所述蛋白质具有如SEQ ID NO：2,8和10所示的氨基酸序列，并且具有将糖基转移到 所述蛋白质使用所述基因产生所述蛋白质的方法。 通过将该基因引入到不具有该基因的植物中，可以稳定黄色的颜料噢one，并且可以获得具有黄色花的植物。
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8.发明授权
公开(公告)号：US5965444A
公开(公告)日：1999-10-12
申请号：US880745
申请日：1997-06-23
CPC分类号： C12N9/2428 , C12N15/65 , C12N15/81 , C12N15/905
摘要： A DNA construct wherein a DNA fragment which is recombinable in yeast chromosomal DNA is directly or indirectly linked at both ends of a DNA fragment which comprises a pair of R sensitive sequences oriented in the same direction and flaking both an R gene placed under the control of an inducible promoter and an expressible selective marker gene, which is a DNA construct designed with the R sensitive sequences non-symmetrically shortened, so that no functionable R sensitive sequence remains after the R sensitive sequence recombination has occurred by expression of the R gene and the selective marker has been removed. Since no functionable R sensitive sequence remains after removal of the selective marker, recombination does not occur again, and thus the same selective marker may be used for multiple insertions of foreign genes.
摘要翻译： 一种DNA构建体，其中在酵母染色体DNA中可重组的DNA片段在DNA片段的两端直接或间接连接，所述DNA片段包含一对沿相同方向取向的R敏感序列， 可诱导的启动子和可表达的选择性标记基因，其是用R敏感序列非对称缩短设计的DNA构建体，使得在通过R基因的表达发生R敏感序列重组后，不存在可操作的R敏感序列 选择性标记已被删除。 由于在去除选择性标记物之后不存在可操作的R敏感序列，因此重新不再次发生，因此相同的选择性标记可用于多次插入外源基因。
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公开(公告)号：US07186886B2
公开(公告)日：2007-03-06
申请号：US10048897
申请日：2001-06-01
申请人： Keiko Sakakibara , Toru Nakayama , Tokuzo Nishino
发明人： Keiko Sakakibara , Toru Nakayama , Tokuzo Nishino
CPC分类号： C12N15/8243 , C12N9/1029
摘要： A protein which has an amino acid sequence shown by SEQ ID NO: 2, 4, 6, 23, 25, 27 or 29, or any of these amino acid sequences modified, and which has activity for transferring an aliphatic acyl group to a glycosyl group at the 5-position of flavonoids, and a gene encoding the same.
摘要翻译： 具有SEQ ID NO：2,4,6,23,25,27或29所示氨基酸序列的氨基酸序列的蛋白质，或这些氨基酸序列的任何修饰，并且具有将脂肪族酰基转移到糖基 在黄酮类化合物的5位上进行组合，编码该基因的基因。
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