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公开(公告)号：US06284546B1
公开(公告)日：2001-09-04
申请号：US08469578
申请日：1995-06-06
IPC分类号： G01N2176
CPC分类号： G01N21/8806 , B01F5/0085 , B01F11/00 , B01F13/0001 , B01F13/0013 , B01F13/0809 , B01F15/0201 , B01F2215/0037 , B01L3/5085 , B01L2400/0415 , B01L2400/0433 , G01N21/8483 , G01N2001/386 , G01N2035/00237 , G01N2035/00554 , Y10T436/110833 , Y10T436/25
摘要： The present invention provides methods and apparatus for mixing two or more liquids and detecting light emitted by the mixture. The method comprises forming a liquid droplet containing two or more liquids on a substantially planar surface in containerless containment on the surface and causing the droplet to deform in an essentially zero air flow environment thereby mixing the liquids and surrounding the planar surface with a reflective housing and photodetector. An apparatus of the invention comprises (a) a substantially planar support, (b) means for dispensing liquids onto the support to form a droplet, and (c) non-evaporative means for causing the droplet to deform without deforming the support thereby mixing the liquids and a two part reflective housing and photodetector which is moveable. The drop can be deformed by, for example, application of acoustic energy or a variable electrostatic field. The methods and apparatus have particular application to the determination of an analyte.
摘要翻译： 本发明提供了混合两种或多种液体并检测由混合物发射的光的方法和装置。 该方法包括在表面上的无容器容纳物中的基本上平坦的表面上形成含有两种或更多种液体的液滴，并使液滴在基本为零的空气流动环境中变形，从而混合液体并围绕平面表面与反射壳体 光电探测器 本发明的装置包括（a）基本上平面的支撑件，（b）用于将液体分配到支撑件上以形成液滴的装置，和（c）非蒸发装置，用于使液滴变形而不使支架变形， 液体和可移动的两部分反射壳体和光电检测器。 可以通过例如施加声能或可变静电场来使液滴变形。 所述方法和装置特别适用于分析物的测定。
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公开(公告)号：US06406913B1
公开(公告)日：2002-06-18
申请号：US08471130
申请日：1995-06-06
IPC分类号： G01T1161
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/81 , Y10S435/968 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/829
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。 光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。 活化的化学发光化合物随后产生光。 所产生的光量与介质中分析物的量有关。 优选地，光敏剂和化学发光化合物中的至少一种与通常是悬浮颗粒的表面相关联，并且特异性结合对成员结合到其上。 还公开了组合物和试剂盒。
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公开(公告)号：US5618732A
公开(公告)日：1997-04-08
申请号：US434617
申请日：1995-05-04
IPC分类号： G01N33/532 , G01N21/78 , G01N33/58 , G01N21/64 , G01N21/76
CPC分类号： G01N33/582 , Y10S435/81 , Y10S436/829 , Y10S436/905 , Y10T436/10
摘要： Methods for labeling a material are disclosed. The methods comprise combining with the material (a) a photosensitizer capable upon irradiation of generating singlet oxygen and (b) a chemiluminescent compound capable of being activated by singlet oxygen wherein the photosensitizer and the chemiluminescent compound are incorporated in a particulate matrix or a non-particulate solid matrix. The particulate matrix can be solid or fluid. The methods allow for generating delayed luminescence, which can be realized upon irradiation of the matrix. The methods have application to the determination of an analyte in a medium suspected of containing the analyte. One method comprises subjecting a medium suspected of containing an analyte to conditions under which a complex of specific binding pair (sbp) members is formed in relation to the presence of the analyte and determining whether the sbp member complex has formed by employing as a label a single composition having both chemiluminescent and photosensitizer properties. Upon activation of the photosensitizer property singlet oxygen is generated and activates the chemiluminescent property. Compositions and kits are also disclosed.
摘要翻译： 公开了标记材料的方法。 所述方法包括与材料（a）结合，所述光敏剂能够在产生单线态氧的照射时和（b）能够被单线态氧活化的化学发光化合物，其中光敏剂和化学发光化合物掺入颗粒基质或非均相中， 颗粒状固体基质。 颗粒基质可以是固体或流体。 这些方法允许产生延迟发光，这可以在照射基质时实现。 该方法可用于确定怀疑含有分析物的介质中的分析物。 一种方法包括使怀疑含有分析物的培养基相对于分析物的存在形成特异性结合对（sbp）成员的复合物的条件，以及通过使用标记a来确定sbp成员复合物是否形成 具有化学发光和光敏剂性质的单一组合物。 激活光敏剂时，产生单线态氧并激活化学发光性能。 还公开了组合物和试剂盒。
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公开(公告)号：US5709994A
公开(公告)日：1998-01-20
申请号：US470862
申请日：1995-06-06
CPC分类号： G01N33/582 , Y10S435/81 , Y10S436/829 , Y10S436/905 , Y10T436/10
摘要： Methods for labeling a material are disclosed. The methods comprise combining with the material (a) a photosensitizer capable upon irradiation of generating singlet oxygen and (b) a chemiluminescent compound capable of being activated by singlet oxygen wherein the photosensitizer and the chemiluminescent compound are incorporated in a particulate matrix or a non-particulate solid matrix. The particulate matrix can be solid or fluid. The methods allow for generating delayed luminescence, which can be realized upon irradiation of the matrix. The methods have application to the determination of an analyte in a medium suspected of containing the analyte. One method comprises subjecting a medium suspected of containing an analyte to conditions under which a complex of specific binding pair (sbp) members is formed in relation to the presence of the analyte and determining whether the sbp member complex has formed by employing as a label a single composition having both chemiluminescent and photosensitizer properties. Upon activation of the photosensitizer property singlet oxygen is generated and activates the chemiluminescent property. Compositions and kits are also disclosed.
摘要翻译： 公开了标记材料的方法。 所述方法包括与材料（a）结合，所述光敏剂能够在产生单线态氧的照射时和（b）能够被单线态氧活化的化学发光化合物，其中光敏剂和化学发光化合物掺入颗粒基质或非均相中， 颗粒状固体基质。 颗粒基质可以是固体或流体。 这些方法允许产生延迟发光，这可以在照射基质时实现。 该方法可用于确定怀疑含有分析物的介质中的分析物。 一种方法包括使怀疑含有分析物的培养基相对于分析物的存在形成特异性结合对（sbp）成员的复合物的条件，以及通过使用标记a来确定sbp成员复合物是否形成 具有化学发光和光敏剂性质的单一组合物。 激活光敏剂时，产生单线态氧并激活化学发光性能。 还公开了组合物和试剂盒。
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公开(公告)号：US5340716A
公开(公告)日：1994-08-23
申请号：US718490
申请日：1991-06-20
IPC分类号： G01N33/569 , G01N33/58 , C12Q1/00
CPC分类号： G01N33/582 , G01N33/56977 , G01N33/58 , G01N33/585
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises providing (1) a medium suspected of containing the analyte, (2) a label reagent comprising a first specific binding pair (sbp) member associated with a photochemically activatable chemiluminescent compound wherein the first sbp member is capable of binding to the analyte or to a second sbp member to form a complex related to the presence of the analyte. The method further comprises photochemically activating the chemiluminescent compound. The amount of luminescence generated by the chemiluminescent compound is detected. The amount thereof is related to the amount of analyte in the medium. Compositions and kits are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括提供（1）怀疑含有分析物的培养基，（2）标记试剂，其包含与光化学活化的化学发光化合物相关的第一特异性结合对（sbp）成员，其中第一sbp成员能够结合分析物 或第二sbp成员以形成与分析物的存在有关的复合物。 该方法还包括光化学活化化学发光化合物。 检测由化学发光化合物产生的发光量。 其量与培养基中分析物的量有关。 还公开了组合物和试剂盒。
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公开(公告)号：US06251581B1
公开(公告)日：2001-06-26
申请号：US07704569
申请日：1991-05-22
IPC分类号： C12Q100
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/81 , Y10S435/968 , Y10S435/971 , Y10S435/973 , Y10S435/975 , Y10S436/829
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括在允许分析物（如果存在）使光敏剂和化学发光化合物接近的条件下处理疑似含有分析物的介质。 光敏剂产生单线态氧并在化学发光化合物接近时激活化学发光化合物。 活化的化学发光化合物随后产生光。 所产生的光量与介质中分析物的量有关。 优选地，光敏剂和化学发光化合物中的至少一种与通常是悬浮颗粒的表面相关联，并且特异性结合对成员结合到其上。 还公开了组合物和试剂盒。
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公开(公告)号：US4891324A
公开(公告)日：1990-01-02
申请号：US925
申请日：1987-01-07
申请人： John Pease , Litai Weng , Hrair Kirakossian , Edwin F. Ullman
发明人： John Pease , Litai Weng , Hrair Kirakossian , Edwin F. Ullman
IPC分类号： G01N33/543 , C09B23/01 , C09B57/00 , G01N33/544 , G01N33/58
CPC分类号： C09B23/0066 , C09B57/007 , G01N33/582 , G01N33/585 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10S436/809 , Y10S436/821 , Y10S436/823 , Y10S436/829 , Y10T428/2982
摘要： Assay methods are provided for determining an analyte in a sample suspected of containing the analyte. The method is carried out using a composition that includes a conjugate of a first sbp member with a particle. A luminescer is reversibly associated with a nonaqueous phase of the particle. Where the first sbp member is not complementary to the analyte, a second sbp member that is capable of binding to the first sbp member is employed. Unbound conjugate is separated from conjugate that is bound to the analyte or to the second sbp member. A reagent for enhancing the detectability of the luminescer is added and the light emission of the luminescer acted on by the reagent is measured.
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公开(公告)号：US07635571B2
公开(公告)日：2009-12-22
申请号：US09732047
申请日：2000-12-07
IPC分类号： G01N33/00
CPC分类号： G01N33/542 , G01N33/531
摘要： Methods are disclosed for determining minute quantities of an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a substrate having an oxidant cleavable linker and a photosensitizer to come into close proximity. The photosensitizer generates singlet oxygen which oxidatively cleaves the linker to form a product which can be detected in a sandwich detection assay such as LOCI. The amount of product detected is related to the amount of analyte in the medium. Compositions, kits, and compounds are also disclosed.
摘要翻译： 公开了用于确定怀疑含有分析物的介质中微量分析物的方法。 一种方法包括处理怀疑含有分析物的介质，使得分析物（如果存在的话）引起具有氧化剂可切割接头和光敏剂的底物接近。 光敏剂产生单线态氧，其氧化地切割接头以形成可在三明治检测测定（例如LOCI）中检测的产物。 所检测的产物量与培养基中分析物的量有关。 还公开了组合物，试剂盒和化合物。
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公开(公告)号：US20080213767A1
公开(公告)日：2008-09-04
申请号：US11869054
申请日：2007-10-09
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/682 , C12Q1/6823 , C12Q2521/319 , C12Q2537/149 , C12Q2521/301 , C12Q2563/101
摘要： A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5′-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5′-nuclease to cleave the oligonucleotide to provide (i) a first fragment that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3′ of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte. The method has particular application to the detection of a polynucleotide analyte such as DNA. Kits for conducting methods in accordance with the present invention are also disclosed.
摘要翻译： 公开了一种修饰寡核苷酸的方法，该方法可用于检测多核苷酸分析物。 在等温条件下，在5'-核酸酶的存在下，寡核苷酸与多核苷酸例如多核苷酸分析物可逆地杂交。 多核苷酸分析物用作识别元件，使得5'-核酸酶能够切割寡核苷酸，以提供（i）与多核苷酸分析物基本上不可杂交的第一片段和（ii）位于3'末端的第3个片段 第一片段（在完整寡核苷酸中）并且与多核苷酸分析物基本上可杂交。 相对于多核苷酸分析物的摩尔量，获得至少100倍摩尔过量的第一片段和/或第二片段。 检测到第一片段和/或第二片段的存在，其存在表明多核苷酸分析物的存在。 该方法特别适用于多核苷酸分析物如DNA的检测。 还公开了用于根据本发明的方法的套件。
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公开(公告)号：US07022529B2
公开(公告)日：2006-04-04
申请号：US10745972
申请日：2003-12-24
IPC分类号： G01N33/543
CPC分类号： G01N33/587 , C12Q1/6816 , Y10T436/10 , C12Q2563/155 , C12Q2523/319
摘要： Methods, compositions and kits are disclosed. The compositions are light emitting and comprise a polymeric matrix having dissolved therein a photoactive compound. The composition has the characteristic that, after activation of the photoactive compound, the rate of decrease in the intensity of light emission at any time during a 20-fold decrease in the intensity is proportional to the intensity of the light emission. In one embodiment the polymeric matrix is comprised of particles of about 20 nm to about 100 μm in diameter to which is bound a specific binding pair member. The particles generally comprise a polymeric matrix having dissolved therein about 1 to about 20% by weight of a dopant. The compositions may be used in methods for determining an analyte. A combination is provided comprising (1) a medium suspected of containing the analyte, (2) and the aforementioned composition. The photoactive substance is activated and the effect of the activating on the optical properties of the combination is detected. The presence and amount of the effect is related to the presence and amount of the analyte in the medium. Also disclosed are kits for use in an assay.
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