公开(公告)号：US20170292939A1

公开(公告)日：2017-10-12

申请号：US15633812

申请日：2017-06-27

申请人： KING FAHD UNIVERSITY OF PETROLEUM AND MINERALS

发明人： Chanbasha BASHEER ,  Mousa Yaser Amayreh

IPC分类号： G01N33/18 ,  G01N30/88 ,  G01N30/06 ,  G01N35/10 ,  G01N30/72 ,  G01N30/24

CPC分类号： G01N33/1826 ,  G01N1/38 ,  G01N30/7206 ,  G01N30/88 ,  G01N2001/4061 ,  G01N2030/062 ,  G01N2030/884 ,  G01N2035/00554 ,  Y10T436/170769 ,  Y10T436/173845 ,  Y10T436/24 ,  Y10T436/255

摘要： An automated dispersive liquid-liquid microextraction method of detecting and quantifying N-nitrosamines in an aqueous sample. The method includes (a) extracting an aqueous solution comprising the N-nitrosamines by mixing an extraction solvent and a dispersive solvent with the aqueous solution, such that the N-nitrosamines, or a portion thereof, re-distribute from the aqueous solution to the extraction solvent, (b) permitting the resulting mixture in (a) to form a two-phase mixture comprising an aqueous phase comprising the aqueous solution with reduced amounts of the N-nitrosamines and an organic phase comprising the extraction solvent with the N-nitrosamines extracted from the aqueous solution, (c) injecting the organic phase, or a portion thereof, into an injection port of a gas chromatograph coupled with at least one mass spectrometer, and (d) analyzing the N-nitrosamines by gas chromatography and mass spectrometry to detect and quantify the concentration of the N-nitrosamines in the aqueous solution.

公开(公告)号：US09623409B2

公开(公告)日：2017-04-18

申请号：US15336735

申请日：2016-10-27

申请人： CUE INC.

发明人： Ayub Khattak ,  Clinton Sever ,  Paul Nelson ,  Ryan Cooper ,  Thomas Congdon ,  Justin Demartino ,  Raphael Shapiro ,  Mark Duncan

IPC分类号： G01N31/22 ,  B01L3/00 ,  B01L7/00 ,  B05D3/00 ,  C12Q1/68 ,  F16K99/00 ,  G01N27/28 ,  G01N27/327 ,  G01N33/543 ,  G01N33/58 ,  G01N35/00 ,  H04M1/725 ,  G01N1/02 ,  G01N21/78 ,  G01N35/10

CPC分类号： B01L3/508 ,  B01F11/0266 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502738 ,  B01L3/5029 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/087 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/023 ,  B01L2300/025 ,  B01L2300/04 ,  B01L2300/044 ,  B01L2300/06 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/12 ,  B01L2300/161 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0439 ,  B01L2400/0487 ,  B01L2400/0677 ,  B01L2400/0683 ,  B05D3/002 ,  B05D2518/00 ,  C12Q1/6825 ,  F16K99/0032 ,  F16K99/0036 ,  F16K2099/0084 ,  G01N1/02 ,  G01N21/78 ,  G01N27/28 ,  G01N27/3271 ,  G01N27/3272 ,  G01N27/3273 ,  G01N33/54306 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/581 ,  G01N35/00029 ,  G01N35/0098 ,  G01N35/1095 ,  G01N2001/027 ,  G01N2001/028 ,  G01N2035/00277 ,  G01N2035/00554 ,  G01N2458/30 ,  H04M1/72527 ,  Y10T137/1797

摘要： Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

公开(公告)号：US09347966B2

公开(公告)日：2016-05-24

申请号：US12867795

申请日：2009-05-01

申请人： Chie Sugiyama ,  Shigeki Matsubara

发明人： Chie Sugiyama ,  Shigeki Matsubara

IPC分类号： G01N1/38 ,  B01F11/02 ,  G01N35/10 ,  B01F7/16 ,  B01F15/00 ,  B01F15/02 ,  G01N35/02 ,  G01N35/00

CPC分类号： G01N35/0092 ,  B01F7/161 ,  B01F11/0283 ,  B01F15/00253 ,  B01F15/00324 ,  B01F15/00435 ,  B01F2015/00629 ,  B01F2015/00636 ,  B01F2015/0221 ,  G01N1/38 ,  G01N35/025 ,  G01N35/1002 ,  G01N2035/00514 ,  G01N2035/00554 ,  G01N2035/0444 ,  Y10T436/25 ,  Y10T436/25625

摘要： In the field of automatic analyzers, as items to be analyzed are increase, various reagents differing in such properties as liquid viscosity and contact angle are being used more frequently, and this trend is expected to continue. Also, reagents now take various forms (e.g., a concentrated reagent to be diluted by the water of an automatic analyzer), and so does dilution water. Such being the case, the invention provides an automatic analyzer capable of sufficient stirring regardless of items to be analyzed. To sufficiently stir a substance to which a reagent has been added, the automatic analyzer is designed to alter stirring conditions after a given amount of time has passed since the addition of that reagent.

摘要翻译： 在自动分析仪领域，随着分析项目的增加，液体粘度和接触角等特性不同的各种试剂的使用频率较高，预计这一趋势将继续下去。 此外，试剂现在采取各种形式（例如，由自动分析仪的水稀释的浓缩试剂），稀释水也是如此。 在这种情况下，本发明提供了能够充分搅拌的自动分析器，而不管要分析的物品。 为了充分搅拌添加有试剂的物质，自动分析装置被设计成在添加该试剂经过规定时间后改变搅拌条件。

公开(公告)号：US20150166979A1

公开(公告)日：2015-06-18

申请号：US14634042

申请日：2015-02-27

申请人： Covaris, Inc.

发明人： James A. Laugharn, JR.

IPC分类号： C12N13/00

CPC分类号： C12N13/00 ,  B01F11/0283 ,  B01J2219/00308 ,  B01J2219/00344 ,  B01J2219/00486 ,  B01J2219/00722 ,  B01J2219/0074 ,  G01N1/286 ,  G01N2035/00554

摘要： Method and apparatus for controlling acoustic treatment of a sample including a liquid. A processing volume in which the sample is acoustically treated may be controlled, e.g., by positioning a suitable element so as to reduce and/or eliminate a headspace size at a sample/gas interface. An interaction between the acoustic energy and the sample may be controlled, e.g., by using a headspace control element positioned at least partially in the sample that helps to reduce splashing or other sample ejection that would otherwise occur.

摘要翻译： 用于控制包括液体的样品的声学处理的方法和装置。 可以例如通过定位合适的元件来控制样品进行声学处理的处理体积，以减少和/或消除样品/气体界面处的顶空尺寸。 可以例如通过使用至少部分定位在样品中的顶空控制元件来控制声能和样品之间的相互作用，所述顶空控制元件有助于减少否则会发生的飞溅或其它样品喷射。

公开(公告)号：US09046505B2

公开(公告)日：2015-06-02

申请号：US13010623

申请日：2011-01-20

申请人： Ryuichiro Ebi ,  Koki Tajima

发明人： Ryuichiro Ebi ,  Koki Tajima

IPC分类号： B01F11/02 ,  G01N35/04 ,  B01F3/12 ,  G01N15/14 ,  B01F3/00 ,  G01N1/40

CPC分类号： G01N35/04 ,  B01F3/1242 ,  B01F11/0266 ,  B01F11/0283 ,  B01F2003/0042 ,  G01N1/38 ,  G01N15/14 ,  G01N15/1459 ,  G01N2001/4094 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2035/00465 ,  G01N2035/00554

摘要： A sample preparation apparatus, comprising: an ultrasonic vibrating unit which applies ultrasonic vibration to a sample including an analyte held in a measurement sample container; a sample preparation unit which prepares a measurement sample by mixing the sample including the analyte to which the ultrasonic vibration is applied and a predetermined reagent; and a container transporting unit which transports the measurement sample container holding the sample including the analyte from the ultrasonic vibrating unit to the sample preparation unit.

摘要翻译： 一种样品制备装置，包括：超声波振动单元，其对包含保存在测量样品容器中的分析物的样品施加超声波振动; 样品制备单元，其通过将包含施加超声波振动的分析物的样品与预定试剂混合来制备测量样品; 以及容器搬送单元，其将将来自超声波振动单元的包含分析物的样本的测定样本容器搬送到样品制备单元。

公开(公告)号：US08975085B2

公开(公告)日：2015-03-10

申请号：US13058828

申请日：2009-08-06

申请人： Jonathan Spencer Howells ,  Kevin Deane

发明人： Jonathan Spencer Howells ,  Kevin Deane

IPC分类号： G01N1/38 ,  G01N1/40 ,  B01F1/00 ,  B01F5/10 ,  B01F11/02 ,  B01F15/04 ,  B01F15/00 ,  G01N35/00

CPC分类号： B01F1/0027 ,  B01F1/0038 ,  B01F5/10 ,  B01F11/0266 ,  B01F11/0283 ,  B01F15/00123 ,  B01F15/0437 ,  B01F2001/0061 ,  G01N1/38 ,  G01N1/4055 ,  G01N2001/383 ,  G01N2001/4061 ,  G01N2035/00554 ,  Y10T436/25

摘要： An extraction system for distributing a test product in an extraction fluid, the system including a flow cell and a pump The flow cell has walls defining an internal passageway having an extraction region for holding the test product and also an upstream end and a downstream end between which the extraction region extends. The internal cross-sectional area of the upstream end is larger than the internal cross-sectional area of the downstream end the and extraction region tapers from the upstream end to the downstream end. The pump has an inlet in fluid communication with the downstream end and an outlet in fluid communication with the upstream end. The pump re-circulates extraction fluid from the downstream end back to the upstream end so as to progressively erode the test product and distribute the test product in the extraction fluid.

摘要翻译： 一种用于将检测产品分配在提取流体中的提取系统，该系统包括流动池和泵。流动池具有限定内部通道的壁，该内部通道具有用于保持测试产品的提取区域，以及用于保持测试产品的上游端和下游端 提取区域延伸。 上游端的内部横截面积大于下游端的内部横截面积，并且提取区域从上游端到下游端逐渐变细。 泵具有与下游端流体连通的入口和与上游端流体连通的出口。 泵将下游端的萃取液再循环回上游端，以逐渐侵蚀试验产品并将试验产品分配到萃取液中。

公开(公告)号：US20140272938A1

公开(公告)日：2014-09-18

申请号：US14203436

申请日：2014-03-10

申请人： Theranos, Inc.

发明人： Alexander Loo ,  Elizabeth A. Holmes

IPC分类号： C12Q1/04 ,  C12Q1/70 ,  G01N33/569

CPC分类号： C12Q1/04 ,  B01F11/0266 ,  B01F11/0283 ,  B01F2215/0037 ,  B01L2300/0609 ,  B01L2400/0439 ,  C12N1/066 ,  G01N1/286 ,  G01N1/38 ,  G01N35/00722 ,  G01N2001/386 ,  G01N2035/00554 ,  G01N2035/00891 ,  G01N2333/24 ,  G01N2333/31

摘要： Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens.

摘要翻译： 提供了包括用于样品制备的超声波发生器的装置，系统和方法。 超声波仪可用于混合，重悬，雾化，分散，分解或除气。 超声波仪器可用于破坏细胞，如样品中的病原体细胞。 样品制备可以包括通过超声处理暴露病原体识别材料以检测，鉴定或测量病原体。 超声波发生器可通过将其尖端接触到含有样品的容器的外壁将超声能量转移到样品溶液。 包括超声波发生器的多用途装置还包括用于附加动作和测定的其它组件。 设备和包括这种设备的系统可以与用于样品测定和分析的系统中的实验室或其他设备通信。 提供了利用这种装置和系统的方法。 改进的样品制备装置，系统和方法可用于分析样品，例如 用于诊断病原体感染的患者。

公开(公告)号：US20140147348A1

公开(公告)日：2014-05-29

申请号：US14171790

申请日：2014-02-04

申请人： HITACHI HIGH-TECHNOLOGIES CORPORATION

发明人： Hajime KATOU ,  Ryo MIYAKE ,  Hiroyasu UCHIDA ,  Katsuhiro KAMBARA ,  Takenori OKUSA ,  Shigenori WATARI ,  Yoichiro SUZUKI ,  Tomonori MIMURA ,  Miki TAKI ,  Akira INAGAKI

IPC分类号： G01N35/10

CPC分类号： G01N35/1002 ,  B01F11/0283 ,  B01F2215/0037 ,  G01N35/025 ,  G01N2035/00554 ,  Y10S707/962 ,  Y10S707/99933 ,  Y10S707/99942 ,  Y10S707/99943 ,  Y10S707/99956

摘要： Chemical analysis apparatus for inserting a sample serving as an analyzable substance and a reagent into a reaction container and agitating by irradiating sound waves to the reaction container, the chemical analysis apparatus including: a piezoelectric element generating sound waves; and a driver for driving the piezoelectric element, and including: a wave form producing device for producing an oscillation waveform having a fundamental frequency of the sound waves to be irradiated; an auxiliary waveform producing device for producing an oscillation wave form having a frequency lower than that of the oscillation waveform; a multiplying circuit creating a multiplied waveform between the waveform and the wave form; and, a power amplifier for power-amplifying the multiplied waveform, wherein it is intermittently irradiated with the sound wave in the reaction container, and further, the wave form producing device has a function capable of frequency-modulation with a prescribed frequency width.

摘要翻译： 用于将用作可分析物质和试剂的样品插入反应容器并通过向反应容器照射声波进行搅拌的化学分析装置，所述化学分析装置包括：产生声波的压电元件; 以及用于驱动压电元件的驱动器，并且包括：波形产生装置，用于产生具有待照射的声波的基频的振荡波形; 用于产生频率低于振荡波形的振荡波形的辅助波形产生装置; 在波形和波形之间产生相乘波形的乘法电路; 以及用于对乘法波形进行功率放大的功率放大器，其中它被反应容器中的声波间歇地照射，此外，波形产生装置具有能够以规定频率宽度进行频率调制的功能。

公开(公告)号：US20140127082A1

公开(公告)日：2014-05-08

申请号：US14153352

申请日：2014-01-13

申请人： Biocartis SA

发明人： Arie R. Van Doorn ,  Michiel De Jong

IPC分类号： B01J19/00

CPC分类号： G01N1/28 ,  B01F11/0283 ,  B01J19/008 ,  G01N2001/2866 ,  G01N2035/00554 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

摘要： An apparatus for irradiating a liquid sample with acoustic energy to generate cavitation in the liquid sample. The apparatus includes a source for emitting high intensity ultrasound waves and is adapted to receive a cartridge having a chamber containing the liquid sample, wherein the high intensity ultrasound waves are focused on a focus position at or near a nucleation element extending at least partially into the chamber.

摘要翻译： 一种用于用液体样品照射液体样品以在液体样品中产生空化的装置。 该装置包括用于发射高强度超声波的源，并且适于接收具有包含液体样品的室的盒，其中高强度超声波被聚焦在成核元件处或其附近的聚焦位置，成核元件至少部分地延伸到 房间。

公开(公告)号：US20130087445A1

公开(公告)日：2013-04-11

申请号：US13675749

申请日：2012-11-13

申请人： Orion Diagnostica Oy

发明人： Juhani Luotola ,  Teuvo Sillanpaa

IPC分类号： B01J19/10

CPC分类号： B01F11/0266 ,  B01F11/0005 ,  G01N2001/2866 ,  G01N2035/00554

摘要： Sonication of a medium for example in an immunoassay is provided by applying sound waves from a transducer to a vessel in which the medium is held by a sonotrode coupled between the transducer and the vessel. The sonotrode has a recess in which the vessel is held, the recess being formed by facing surfaces of a plurality of protruding portions of the sonotrode separated by slits and arranged around the recess. The sonotrode is coupled to the vessel by dry contact with the vessel without any coupling layers therebetween. The use of such a sonotrode provides the advantages of allowing effective sonication with relatively low energy loss and with a low temperature elevation.

摘要翻译： 通过将来自换能器的声波施加到容器中来提供例如在免疫测定中的介质的超声处理，其中介质由连接在换能器和容器之间的超声波驱动器保持。 超声波发生器具有容器保持的凹部，凹部由通过狭缝分开并布置在凹部周围的超声焊丝的多个突出部的相对表面形成。 超声波发生器通过与容器干接触而耦合到容器，而在它们之间没有任何耦合层。 使用这样的超声波发生器提供了以相当低的能量损失和低温升高来进行有效超声处理的优点。