公开(公告)号：US08195775B2

公开(公告)日：2012-06-05

申请号：US12450684

申请日：2007-04-06

申请人： Zhi Gang Zhang ,  Kui Gao ,  Jian Song Li

发明人： Zhi Gang Zhang ,  Kui Gao ,  Jian Song Li

IPC分类号： G06F15/16

CPC分类号： H04L47/10 ,  H04L29/12283 ,  H04L47/125 ,  H04L61/2015 ,  H04L61/2061

摘要： An enhanced method and apparatus for reducing congestion in dynamic host configuration protocol network system are provided. The congestion reduction method comprises steps of receiving a DHCP request from a DHCP client; and stopping responding to a certain number of DHCPDISCOVER message. And the dynamic host configuration protocol server comprises at least one distributed server, at which the contents of the network service provider server are backed up.

摘要翻译： 提供了一种用于减少动态主机配置协议网络系统拥塞的增强方法和装置。 所述拥塞减少方法包括从DHCP客户端接收DHCP请求的步骤; 并停止响应一定数量的DHCPDISCOVER消息。 并且动态主机配置协议服务器包括至少一个分布式服务器，在该服务器上备份网络服务提供商服务器的内容。

公开(公告)号：US08911946B2

公开(公告)日：2014-12-16

申请号：US12340622

申请日：2008-12-19

申请人： Michael M. Becker ,  Kui Gao ,  Wai-Chung Lam

发明人： Michael M. Becker ,  Kui Gao ,  Wai-Chung Lam

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q2600/16 ,  C12Q2545/114 ,  C12Q2545/101 ,  C12Q2531/113

摘要： Method of detecting methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) in a nucleic acid coamplification assay. The invention advantageously reduces the incidence of false-positive MRSA determinations in real-time assays by requiring satisfaction of a threshold criterion that excludes certain co-infections from the MRSA determination. The invention further provides for determination of MSSA, even when the MSSA is present in combination with methicillin-resistant coagulase-negative (MR-CoNS) bacteria at high or low levels.

摘要翻译： 在核酸共扩增测定中检测耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感性金黄色葡萄球菌（MSSA）的方法。 本发明有利地通过要求满足从MRSA测定中排除某些共感染的阈值标准来降低实时分析中假阳性MRSA测定的发生率。 本发明还提供MSSA的测定，即使MSSA与高水平或低水平的耐甲氧西林凝固酶阴性（MR-CoNS）细菌组合存在。

公开(公告)号：US09752201B2

公开(公告)日：2017-09-05

申请号：US14232874

申请日：2012-07-13

申请人： Kui Gao ,  Jeffrey M. Linnen ,  Kurt Craft Norton ,  Patricia C. Gordon ,  Dat Do ,  Tan N. Le

发明人： Kui Gao ,  Jeffrey M. Linnen ,  Kurt Craft Norton ,  Patricia C. Gordon ,  Dat Do ,  Tan N. Le

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C07H21/02

CPC分类号： C12Q1/706 ,  C12Q1/701 ,  C12Q2600/16 ,  Y02A50/54

摘要： Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

公开(公告)号：US20060188912A1

公开(公告)日：2006-08-24

申请号：US11356613

申请日：2006-02-17

申请人： Kui Gao ,  Michael Becker ,  Wen Wu ,  Jeffrey Linnen

发明人： Kui Gao ,  Michael Becker ,  Wen Wu ,  Jeffrey Linnen

IPC分类号： C12Q1/68

CPC分类号： C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2527/119

摘要： Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.

公开(公告)号：US08420317B2

公开(公告)日：2013-04-16

申请号：US13307991

申请日：2011-11-30

申请人： Kui Gao ,  Michael M. Becker ,  Wen Wu ,  Jeffrey M. Linnen

发明人： Kui Gao ,  Michael M. Becker ,  Wen Wu ,  Jeffrey M. Linnen

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2527/119

摘要： Method of processing a biological sample to yield nucleic acid appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.

摘要翻译： 处理生物样品以产生适合用于随后的体外核酸扩增反应的核酸的方法。 该方法包括快速，瞬时暴露于碱性条件，这可以通过将碱性溶液与包含洗涤剂的pH缓冲溶液和待测试的生物样品混合使用体外扩增来存在特定的核酸物种来实现。 本发明的方法有利地可以改善某些靶核酸的检测，而不会显着损害其他目标核酸的可检测性。 该方法特别适用于同时制备可用于多重扩增反应的RNA和DNA模板。

公开(公告)号：US08385432B2

公开(公告)日：2013-02-26

申请号：US11920819

申请日：2006-05-03

申请人： Ying Chen ,  Jiefu Zhai ,  Kui Gao ,  Kai Xie

发明人： Ying Chen ,  Jiefu Zhai ,  Kui Gao ,  Kai Xie

IPC分类号： H04N7/12

CPC分类号： H04N19/615 ,  H04N19/103 ,  H04N19/105 ,  H04N19/13 ,  H04N19/159 ,  H04N19/172 ,  H04N19/33 ,  H04N19/59 ,  H04N19/61 ,  H04N19/63

摘要： Scalable video coding based on H.264/AVC uses motion compensation, which results in motion vectors and residual pictures. To optimize the encoding of the residual pictures of the enhancement-layer, they can be predicted from the base-layer. This requires complex encoders and decoders. Simplified residual prediction techniques are provided that focus on reducing the encoding and decoding complexity of the spatial scalable enhancement-layer with optimized coding efficiency. The method for encoding video data containing high-pass frames and low-pass frames comprises the steps of encoding the low-pass frames, wherein residual prediction may be used, splitting the high-pass frames into two interleaving frame groups, encoding the frames of the first of said frame groups, wherein residual prediction may be used, and encoding the frames of the second of the frame groups using an encoding method without residual prediction.

公开(公告)号：US08071301B2

公开(公告)日：2011-12-06

申请号：US12827605

申请日：2010-06-30

申请人： Kui Gao ,  Michael M. Becker ,  Wen Wu ,  Jeffrey M. Linnen

发明人： Kui Gao ,  Michael M. Becker ,  Wen Wu ,  Jeffrey M. Linnen

IPC分类号： C12Q1/68

CPC分类号： C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2527/119

摘要： Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.

摘要翻译： 制备适合用于随后体外核酸扩增反应的生物样品的方法。 该方法包括快速，瞬时暴露于碱性条件，这可以通过将碱性溶液与包含洗涤剂的pH缓冲溶液和待测试的生物样品混合使用体外扩增来存在特定的核酸物种来实现。 本发明的方法有利地可以改善某些靶核酸的检测，而不会显着损害其他目标核酸的可检测性。 该方法特别适用于同时制备可用于多重扩增反应的RNA和DNA模板。

公开(公告)号：US20140255914A1

公开(公告)日：2014-09-11

申请号：US14232874

申请日：2012-07-13

申请人： Kui Gao ,  Jeffrey M. Linnen ,  Kurt Craft Norton ,  Patricia C. Gordon ,  Dat Do ,  Tan N. Le

发明人： Kui Gao ,  Jeffrey M. Linnen ,  Kurt Craft Norton ,  Patricia C. Gordon ,  Dat Do ,  Tan N. Le

IPC分类号： C12Q1/70

CPC分类号： C12Q1/706 ,  C12Q1/701 ,  C12Q2600/16 ,  Y02A50/54

摘要： Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

摘要翻译： 公开了对人细小病毒基因组DNA特异性的核酸低聚物。 公开了用于在生物样品中扩增和检测人细小病毒基因型1,2和3核酸的测定法。 公开了用于扩增和检测人类生物样本中人细小病毒基因型1,2和3基因组DNA的存在的组合物。

公开(公告)号：US07803581B2

公开(公告)日：2010-09-28

申请号：US12409818

申请日：2009-03-24

申请人： Kui Gao ,  Michael M. Becker ,  Wen Wu ,  Jeffrey M. Linnen

发明人： Kui Gao ,  Michael M. Becker ,  Wen Wu ,  Jeffrey M. Linnen

IPC分类号： C12P19/34 ,  C07H1/08

CPC分类号： C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2527/119

摘要： Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.

摘要翻译： 制备适合用于随后体外核酸扩增反应的生物样品的方法。 该方法包括快速，瞬时暴露于碱性条件，这可以通过将碱性溶液与包含洗涤剂的pH缓冲溶液和待测试的生物样品混合使用体外扩增来存在特定的核酸物种来实现。 本发明的方法有利地可以改善某些靶核酸的检测，而不会显着损害其他目标核酸的可检测性。 该方法特别适用于同时制备可用于多重扩增反应的RNA和DNA模板。

公开(公告)号：US20100198910A1

公开(公告)日：2010-08-05

申请号：US12450684

申请日：2007-04-06

申请人： Zhigang Zhang ,  Kui Gao ,  Jilan Li Song

发明人： Zhigang Zhang ,  Kui Gao ,  Jilan Li Song

IPC分类号： G06F15/16

CPC分类号： H04L47/10 ,  H04L29/12283 ,  H04L47/125 ,  H04L61/2015 ,  H04L61/2061

摘要： An enhanced method and apparatus for reducing congestion in dynamic host configuration protocol network system are provided. The congestion reduction method comprises steps of receiving a DHCP request from a DHCP client; and stopping responding to a certain number of DHCPDISCOVER message. And the dynamic host configuration protocol server comprises at least one distributed server, at which the contents of the network service provider server are backed up.

摘要翻译： 提供了一种用于减少动态主机配置协议网络系统拥塞的增强方法和装置。 所述拥塞减少方法包括从DHCP客户端接收DHCP请求的步骤; 并停止响应一定数量的DHCPDISCOVER消息。 并且动态主机配置协议服务器包括至少一个分布式服务器，在该服务器上备份网络服务提供商服务器的内容。