公开(公告)号：US20240279828A1

公开(公告)日：2024-08-22

申请号：US18565339

申请日：2022-05-31

申请人： GENSCRIPT USA INC. ,  CUSTOMARRAY, INC. ,  NANJING GENSCRIPT BIOTECH CO., LTD.

发明人： Feichi HU ,  Michael W. REED ,  John COOPER ,  Jackson KELLOCK ,  Cheng-Hsien WU

IPC分类号： C25B11/085 ,  B01J19/00 ,  C07C245/20 ,  C25B3/07 ,  C25B3/09 ,  C25B3/29 ,  C25B11/052 ,  C25B11/061 ,  C25B11/095 ,  C25D9/02 ,  C25F1/00

CPC分类号： C25B11/085 ,  B01J19/0046 ,  C07C245/20 ,  C25B3/07 ,  C25B3/09 ,  C25B3/29 ,  C25B11/052 ,  C25B11/061 ,  C25B11/095 ,  C25D9/02 ,  C25F1/00 ,  B01J2219/00596 ,  B01J2219/00713 ,  B01J2219/00722

摘要： Functionalized aryldiazonium salts and films formed by electrografting of functionalized aryldiazonium salts are provided. Methods for purifying functionalized aryldiazonium salts and for coating solid support systems with functionalized aryldiazonium salts are also provided. These coated solid support systems can be used, for example, in methods of oligonucleotide synthesis.

公开(公告)号：US11898186B1

公开(公告)日：2024-02-13

申请号：US18069123

申请日：2022-12-20

申请人： GENSCRIPT USA INC.

发明人： Cheng-Hsien Wu ,  Fengmei Pi ,  Robert Dempcy ,  Shambhavi Shubham ,  Kristine Bielecki ,  Aaron Ball

IPC分类号： A61K31/7115 ,  A61K48/00 ,  C12P19/34 ,  C12N9/12 ,  C12N9/16 ,  C12N9/64

CPC分类号： C12P19/34 ,  C12N9/1276 ,  C12N9/16 ,  C12N9/6472 ,  C12Y207/07049 ,  C12Y301/03002 ,  C12Y304/22056

摘要： A method for in vitro transcription of a DNA template into RNA includes providing a mixture containing a buffer substance, ribonucleoside triphosphates (NTPs), one or more magnesium salts in a concentration of from about 2 mM to about 60 mM, the DNA template, and a recombinant RNA polymerase, and incubating the reaction mixture at from about 25° C. to about 40° C. for from about 1 hour to about 12 hours thereby producing the RNA. A method for in vitro transcription includes providing a DNA template and a cap analogue that binds to −1 and/or +1 nucleotides of promoter for in vitro transcription, thus producing more full length mRNAs, allowing for more flexibility on the choice of first mRNA base, and providing +2 position open for custom sequence.

公开(公告)号：US20240209018A1

公开(公告)日：2024-06-27

申请号：US18551834

申请日：2022-03-22

申请人： GENSCRIPT USA INC. ,  CustomArray, Inc.

发明人： Michael W. REED ,  Cheng-Hsien WU ,  John COOPER ,  Robert O. DEMPCY

IPC分类号： C07H21/04 ,  C07C247/04 ,  C07C275/10 ,  C07D491/18 ,  C07F9/24 ,  C25B3/05 ,  C25B3/25 ,  C25B3/29

CPC分类号： C07H21/04 ,  C07C247/04 ,  C07C275/10 ,  C07D491/18 ,  C07F9/2458 ,  C25B3/05 ,  C25B3/25 ,  C25B3/29

摘要： Provided herein are methods and compositions for oligonucleotide synthesis utilizing universal linker phosphoramidites. Methods and reagents are described with DNA synthesis using controlled pore glass (CPG) solid supports, and on platinum coated electrodes for electrochemical DNA synthesis. The universal linkers can be used as spacers in single-column PCR primer synthesis to generate 2 strands with free 3′-hydroxy termini after cleavage. The methods and compositions utilize a solid support system for synthesis of oligonucleotides, wherein the support has platinum electrodes and a universal linker, optionally wherein the platinum electrode is coated with an amine. The methods and compositions further describe use of universal linker phosphoramidites and the platinum electrode is coated with a monosaccharide, or a disaccharide.

公开(公告)号：US20240060107A1

公开(公告)日：2024-02-22

申请号：US18069123

申请日：2022-12-20

申请人： GENSCRIPT USA INC.

发明人： Cheng-Hsien WU ,  Fengmei PI ,  Robert DEMPCY ,  Shambhavi SHUBHAM ,  Kristine BIELECKI ,  Aaron BALL

IPC分类号： C12P19/34 ,  C12N9/12 ,  C12N9/64 ,  C12N9/16

CPC分类号： C12P19/34 ,  C12N9/1276 ,  C12Y207/07049 ,  C12N9/6472 ,  C12Y304/22056 ,  C12N9/16 ,  C12Y301/03002

摘要： A method for in vitro transcription of a DNA template into RNA includes providing a mixture containing a buffer substance, ribonucleoside triphosphates (NTPs), one or more magnesium salts in a concentration of from about 2 mM to about 60 mM, the DNA template, and a recombinant RNA polymerase, and incubating the reaction mixture at from about 25° C. to about 40° C. for from about 1 hour to about 12 hours thereby producing the RNA. A method for in vitro transcription includes providing a DNA template and a cap analogue that binds to −1 and/or +1 nucleotides of promoter for in vitro transcription, thus producing more full length mRNAs, allowing for more flexibility on the choice of first mRNA base, and providing +2 position open for custom sequence.

公开(公告)号：US20230332134A1

公开(公告)日：2023-10-19

申请号：US18042066

申请日：2021-08-17

申请人： Nanjing GenScript Biotech Co., Ltd. ,  GenScript USA Inc.

发明人： Stephen Richard Hughes ,  Xiaoli Fu ,  Yifan Li ,  Yuzhuo He ,  Hong Li ,  Cheng-Hsien Wu ,  Chuan Sun

IPC分类号： C12N15/10 ,  C12M1/00

CPC分类号： C12N15/1006 ,  C12M47/06

摘要： Provided is an automated system for preparing purified plasmid DNA in commercial scale.