公开(公告)号：US11779666B2

公开(公告)日：2023-10-10

申请号：US18124582

申请日：2023-03-22

申请人： INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Fengzhong Wang ,  Litao Tong ,  Zhiying Chen ,  Bei Fan ,  Lili Wang ,  Long Li

IPC分类号： A61L2/00 ,  A23L3/22 ,  A23L3/00 ,  B04B11/02 ,  B04B11/06 ,  B04B13/00 ,  A61L2/26 ,  A61L2/24 ,  A23B9/02

CPC分类号： A61L2/0023 ,  A23B9/025 ,  A23L3/001 ,  A23L3/003 ,  A23L3/225 ,  A61L2/0017 ,  A61L2/24 ,  A61L2/26 ,  B04B11/02 ,  B04B11/06 ,  B04B13/00 ,  A61L2202/11 ,  A61L2202/14 ,  A61L2202/15 ,  A61L2202/21

摘要： A continuous differential-pressure steam sterilization system for a powder, and belongs to the field of material sterilization includes: a superheated steam generation system, a steam pressure and flow rate control system, a quantitative feeding system, an instantaneous differential-pressure sterilization system, a dust explosion suppression system, a sterile cooling system, a primary gas-solid separation system, a secondary gas-solid separation system, a sterile storage system, a steam recovery and reheating system, and a condensate recovery system. The continuous differential-pressure steam sterilization system shortens the thermal contact time and mainly accumulates the heat on the surface of the powder, rather than in the center of the powder, which reduces the damage to the nutritional quality of the powder. Comprehensive treatment methods such as superheated steam, temperature compensation and non-sticky inner lining are adopted to reduce the problem of powder binding, agglomeration, and even blocking in the pipe of the system.

公开(公告)号：US20230218786A1

公开(公告)日：2023-07-13

申请号：US18124582

申请日：2023-03-22

申请人： INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Fengzhong WANG ,  Litao TONG ,  Zhiying CHEN ,  Bei FAN ,  Lili WANG ,  Long LI

IPC分类号： A61L2/00 ,  A23B9/02 ,  A23L3/22 ,  A23L3/00 ,  B04B11/02 ,  B04B11/06 ,  B04B13/00 ,  A61L2/26 ,  A61L2/24

CPC分类号： A61L2/0023 ,  A23B9/025 ,  A23L3/225 ,  A23L3/003 ,  A23L3/001 ,  B04B11/02 ,  B04B11/06 ,  B04B13/00 ,  A61L2/26 ,  A61L2/24 ,  A61L2/0017 ,  A61L2202/15 ,  A61L2202/11 ,  A61L2202/14 ,  A61L2202/21

摘要： A continuous differential-pressure steam sterilization system for a powder, and belongs to the field of material sterilization includes: a superheated steam generation system, a steam pressure and flow rate control system, a quantitative feeding system, an instantaneous differential-pressure sterilization system, a dust explosion suppression system, a sterile cooling system, a primary gas-solid separation system, a secondary gas-solid separation system, a sterile storage system, a steam recovery and reheating system, and a condensate recovery system. The continuous differential-pressure steam sterilization system shortens the thermal contact time and mainly accumulates the heat on the surface of the powder, rather than in the center of the powder, which reduces the damage to the nutritional quality of the powder. Comprehensive treatment methods such as superheated steam, temperature compensation and non-sticky inner lining are adopted to reduce the problem of powder binding, agglomeration, and even blocking in the pipe of the system.

公开(公告)号：US20220088103A1

公开(公告)日：2022-03-24

申请号：US16627728

申请日：2019-07-18

申请人： INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Qiang WANG ,  Aimin SHI ,  Lei DENG ,  Rui GUO ,  Hui HU ,  Li LIU ,  Hongzhi LIU ,  Qin GUO

IPC分类号： A61K36/48

摘要： A peanut stem and leaf extract contains at least naringenin-4,7′-dimethyl ether, 2′-O-methylisoliquiritigenin, linalool and ferulic acid. The peanut stem and leaf extract can be prepared by pulverizing dried peanut stems and leaves to a micropowder level, followed by water extraction. The water extraction includes placing the pulverized peanut stems and leaves in water at a temperature of 75 to 85° C. for 4 to 5 hours. The preparation method can efficiently extract active substances in peanut stems and leaves, and the peanut stem and leaf extract can effectively improve sleep and treat insomnia.

公开(公告)号：US20210388392A1

公开(公告)日：2021-12-16

申请号：US17243079

申请日：2021-04-28

申请人： Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences

发明人： Xiaoyang PANG ,  Weixun LI ,  Lan YANG ,  Jiaping LV ,  Jing LU ,  Shuwen ZHANG ,  Qing ZHU ,  Chen XU

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/683

摘要： The present invention discloses a CRISPR/LpCas9 gene editing system and application thereof, the CRISPR/LpCas9 gene editing system includes a complex of LpCas9 protein and sgRNA, which can accurately locate a target DNA sequence and cleave DNA double strands. The LpCas9 protein has an amino acid sequence shown in SEQ ID NO:1; and the sgRNA has a nucleotide sequence shown in SEQ ID NO:2, or a modified sgRNA sequence based on SEQ ID NO: 2. The present invention can effectively solve the problems of heterologous codon bias and cytotoxicity in the application of SpCas9 in Lactobacillus paracasei. The gene editing is performed in cells or in vitro by introducing artificially designed CRISPR RNA and a repair template, which solves the problem of low electroporation efficiency caused by the strains' characteristics or relatively large carried plasmids, and has broad application prospects in the field of gene editing.

公开(公告)号：US11134697B2

公开(公告)日：2021-10-05

申请号：US16362587

申请日：2019-03-22

申请人： Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences

发明人： Chunhui Zhang ,  Wei Jia ,  Xiaojie Qin ,  Qingshan Shen ,  Hongru Zhang

IPC分类号： A23C9/18 ,  A23C9/152

摘要： The present invention discloses a preparation method of instant bone milk, including: washing edible animal bones and then crushing, adding a certain amount composite effect enhancer, performing two high pressure hydrolysis-instantaneous pressure relief in high pressure hydrolysis chamber, collecting liquid-phase materials, performing centrifugation for degreasing, wet sieving, homogenizing, spray drying to obtain powdered instant bone milk. A device for preparation method of instant bone milk comprises a high-pressure hydrolysis tank, a grading conveying pipe and a gas-liquid separator. The instant bone milk prepared by the present invention has stable chemical structure, uniform particle size distribution and regular normal distribution, high protein solubility and good solution stability. And the preparation method has simple process, low cost equipment and good versatility.

公开(公告)号：US11976038B1

公开(公告)日：2024-05-07

申请号：US18399457

申请日：2023-12-28

申请人： INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Qiang Wang ,  Qin Guo ,  Tian Li ,  Xiaoning Hu ,  Manzhu Liang ,  Yang Qu ,  Zhenyuan Li

IPC分类号： C07C67/62 ,  C07C37/82 ,  C07C37/88 ,  C07C67/56

CPC分类号： C07C67/62 ,  C07C37/82 ,  C07C37/88 ,  C07C67/56

摘要： The present disclosure provides a preparation method of resveratrol nervonic acid ester, comprising the following steps of: step 1: weighing resveratrol, a nervonic acid and thiocarbonyldiimidazole according to a molar ratio of 1:1:0.8 to 1:7:11.2 in a reactor; step 2: adding a certain amount of solvent into the reactor to completely dissolve the reactants; and step 3: stirring for reaction at room temperature for 30 min to 180 min. The preparation method is safe, efficient and clean, and enables synthesis of the resveratrol nervonic acid ester with high conversion rate, high purity and high yield.

公开(公告)号：US11795242B1

公开(公告)日：2023-10-24

申请号：US18336627

申请日：2023-06-16

申请人： INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Jinfeng Bi ,  Jianyong Yi ,  Shuhan Feng ,  Xin Jin ,  Xinye Wu ,  Youchuan Ma

IPC分类号： C08B37/00

CPC分类号： C08B37/0045

摘要： Disclosed is a method for regulating a porous structure and texture property of a freeze-dried pectin aerogel, which comprises the following steps of step 1: preparation of pectin/starch solution by mixing pectin powder and a gelatinized starch solution to form a stable pectin/starch mixed solution, a concentration of the pectin being 0.05% to 0.2% (w/v); step 2: induction of gel reaction by adding D-(+)-Glucono-δ-lactone and calcium carbonate into the stable pectin-starch mixed solution; and step 3: vacuum freeze of the starch/pectin composite gel subjected to the gel reaction to obtain the freeze-dried pectin/starch aerogel with an adjustable porous structure and an improved texture characteristic. Controllable regulation of the porous structure of the freeze-dried pectin aerogel is achieved by the method without complex pretreatment. Raw material ingredients applied in the method are cheap, easy-to-access, and has low-toxicity.

公开(公告)号：US20220308068A1

公开(公告)日：2022-09-29

申请号：US17310842

申请日：2020-08-20

申请人： Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences

发明人： Chunhui ZHANG ,  Mengliang YE ,  Yujie GUO ,  Qiankun ZHENG

IPC分类号： G01N33/68 ,  G01N30/06 ,  G01N30/88

摘要： The disclosure discloses a biomarker in osteoporosis intervention therapy by bone peptide, the biomarker including a lipid and lipid-like molecule, an organic acid and its derivative, and/or a neurotransmitter, wherein the lipid and lipid-like molecule includes one or more of taurine, arachidonic acid, 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphoethanolamine, (4Z, 7Z,10Z,13Z,16Z,19Z)-4,7,10,13,16,19-docosahexaenoic acid, 1-stearoyl-2-hydroxy-sn-glycerol-3-phosphocholine, taurodeoxycholic acid, taurochenodeoxycholate or taurocholic acid. The disclosure discloses a screening method of a biomarker in the anti-osteoporosis activity of bone peptide. The disclosure discloses a use of the biomarker.

公开(公告)号：US10375981B2

公开(公告)日：2019-08-13

申请号：US15791845

申请日：2017-10-24

申请人： Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences

发明人： Qiang Wang ,  Li Liu ,  Jinchuang Zhang ,  Song Zhu ,  Hongzhi Liu ,  Aimin Shi ,  Hui Hu

IPC分类号： A23L25/00 ,  A23L5/00 ,  A23P30/20 ,  A23J3/14 ,  A23J3/22

摘要： High-moisture TPP and its preparation by crushing and mixing low-temperature defatted peanut protein powder and extrusion texturizing at 60° C. to 80° C. in the feeding zone, 90° C. to 100° C. in the mixing zone, 120° C. to 160° C. in the melting zone, 90° C. to 150° C. at the cooling die, and 50° C. to 100° C. in the molding zone. Water is added online during extrusion to adjust the moisture content to 45% to 60%. The cooled product is high-moisture TPP that has a moisture content of 55% or more, bright white color, fragrant taste, abundant fibrous structure, is immediately edible, and can be used as a substitute for meat in the manufacture of chicken dices, pulled meat, vegetarian sausage and the like. The method fully utilizes raw materials, has almost no waste, is continuous, has a high process integration, and low energy consumption, and it can enhance the value of peanut protein powder.

公开(公告)号：US11134704B2

公开(公告)日：2021-10-05

申请号：US16170845

申请日：2018-10-25

申请人： Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences

发明人： Chunhui Zhang ,  Wei Jia ,  Xia Li ,  Hongru Zhang ,  Xiaojie Qin

IPC分类号： A23J1/10 ,  A23J3/04 ,  B01D39/08 ,  B01D39/10 ,  B01D45/08 ,  B02C17/08 ,  B09B3/00 ,  A23L5/10 ,  A23L5/20 ,  F22B37/26 ,  B01J3/00 ,  B01J3/02 ,  B01J3/03

摘要： The invention discloses a liquefaction device of hard bone, comprising: outer cavity having upper end detachably sealed with upper cover, and lower end openable/closable sealed with lower sealing cover, first liquefaction cavity coaxially slidably disposed in outer cavity, stainless steel cage disposed coaxially in first liquefaction cavity, second liquefaction cavity fixed to outer sidewall of outer cavity such that lower end portion of outer cavity is located in second liquefaction cavity. The invention also provides a method for co-production of bone collagen polypeptide and ultrafine bone powder based on liquefaction device, including: selecting hard bones, crushing; performing first-stage and second-stage liquefaction to obtain liquid phase and solid phase; centrifuging, concentrating, drying liquid phase to obtain bone collagen polypeptide; drying, coarsely and superfine pulverizing solid phase to obtain ultrafine bone powder. The invention has effects of simplifying process, improving production efficiency, and reducing production and equipment investments.