公开(公告)号：US10821172B2

公开(公告)日：2020-11-03

申请号：US16074724

申请日：2017-11-03

申请人： PULIKE BIOLOGICAL ENGINEERING, INC.

发明人： Kegong Tian ,  Yi Cheng ,  Wenqiang Pang ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： A61K39/235 ,  A61K39/17 ,  A61K39/145 ,  A61K39/215 ,  A61P31/20 ,  A61K39/12 ,  A61K39/00

摘要： The present disclosure provides a vaccine composition comprising an immune amount of Fiber protein of egg drop syndrome virus or an immune amount of a live vector recombined with gene of the Fiber protein of egg drop syndrome virus and a veterinarily acceptable carrier.

公开(公告)号：US10548972B2

公开(公告)日：2020-02-04

申请号：US14901981

申请日：2015-01-06

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Xuke Zhang ,  Jinzhong Sun ,  Feifei Tan

IPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/245 ,  C12N7/04 ,  A61K39/12 ,  A61K45/06

摘要： The present invention provides an attenuated strain of porcine pseudorabies virus (PRV), in which said attenuated strain of PRV is a variant strain of PRV with inactivation of gI/gE/11K/28K proteins. In addition, the present invention also provides a vaccine composition comprising the attenuated strain of PRV as an antigen, a preparation method and use thereof. Proved by immunogenicity and pathogenicity testing of the live vaccine, said live PRV vaccine can provide a good protection for pigs with no clinical signs observed, indicating excellent immune protection.

公开(公告)号：US20160114029A1

公开(公告)日：2016-04-28

申请号：US14980673

申请日：2015-12-28

申请人： PULIKE BIOLOGICAL ENGINEERING, INC.

发明人： XUKE ZHANG ,  JINZHONG SUN ,  RUI WU ,  FEIFEI TAN ,  CHAOYONG BAI ,  KEGONG TIAN

IPC分类号： A61K39/245 ,  C12N7/00 ,  C07K14/005

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K2039/5252 ,  A61K2039/552 ,  C07H21/04 ,  C12N7/00 ,  C12N7/04 ,  C12N2710/16721 ,  C12N2710/16722 ,  C12N2710/16734 ,  C12N2710/16761 ,  C12N2710/16771

摘要： Provided in the embodiments of the present invention is a vaccine composition including an immune amount of attenuated live vaccine, inactivated vaccine, subunit vaccine, synthetic vaccine, or genetically engineered vaccine of the porcine pseudorabies virus strain. The vaccine composition can effectively induce antibody generation, and prevent infections of virulent strains of the porcine pseudorabies virus, and provides effective protection for pigs.

摘要翻译： 在本发明的实施方案中提供了包含免疫量的减毒活疫苗，灭活疫苗，亚单位疫苗，合成疫苗或猪伪狂犬病病毒株的遗传工程化疫苗的疫苗组合物。 疫苗组合物可以有效地诱导抗体产生，并防止猪伪狂犬病病毒毒株的感染，并为猪提供有效的保护。

公开(公告)号：US20220096620A1

公开(公告)日：2022-03-31

申请号：US17421541

申请日：2019-01-15

申请人： PULIKE BIOLOGICAL ENGINEERING, INC.

发明人： Kegong Tian ,  Yan Xiao ,  Wenqiang Pang ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： A61K39/135 ,  C07K14/005 ,  A61P31/14

摘要： The present disclosure provides a type O foot-and-mouth disease virus-like particle antigen, wherein the type O foot-and-mouth disease virus-like particle antigen is type O CATHAY type foot-and-mouth disease virus-like particle antigen, and the type O CATHAY type foot-and-mouth disease virus-like particle antigen is assembled by VP0, VP3 and VP1 antigen proteins of type O CATHAY type foot-and-mouth disease virus. The type O foot-and-mouth disease virus-like particle antigen of the present disclosure has good immunogenicity. The prepared vaccine can produce complete protection against the O-type foot-and-mouth disease virus on the 14th day after immunization. The antibody titer produced is higher than that of the commercial inactivated vaccine, and the duration of immune protection can be maintained for at least 133 days. The disclosure also relates to the prepared vaccine composition, preparation method and use thereof.

公开(公告)号：US10449246B2

公开(公告)日：2019-10-22

申请号：US15738462

申请日：2015-11-12

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Tongyan Wang ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： A61K39/245 ,  A61P31/22 ,  A61K39/39 ,  A61K39/12 ,  C07K14/005 ,  A61K39/00

摘要： The present disclosure provides a PRV gB protein fragment, or a conservative variant or active fragment thereof, the gB protein fragment has a high level of expression, the subunit vaccine antigen prepared from the gB protein fragment has a better immune effect than a subunit vaccine antigen prepared from gB protein. The invention also provides a preparation method of a subunit vaccine by using the gB protein fragment alone, or the gB protein fragment together with gD protein. This vaccine has a simple preparation method and provides excellent protection against disease caused by the porcine pseudorabies virus.

公开(公告)号：US10201605B2

公开(公告)日：2019-02-12

申请号：US15213662

申请日：2016-07-19

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Xuke Zhang ,  Jinzhong Sun ,  Feifei Tan

IPC分类号： A61K39/00 ,  C12N7/00 ,  A61K39/245 ,  C12N7/04 ,  A61K39/12 ,  A61K45/06

摘要： The present invention provides an attenuated strain of porcine pseudorabies virus (PRV), in which said attenuated strain of PRV is a variant strain of PRV with inactivation of gI/gE/11K/28K proteins. In addition, the present invention also provides a vaccine composition comprising the attenuated strain of PRV as an antigen, a preparation method and use thereof. Proved by immunogenicity and pathogenicity testing of the live vaccine, said live PRV vaccine can provide a good protection for pigs with no clinical signs observed, indicating excellent immune protection.

公开(公告)号：US20230149529A1

公开(公告)日：2023-05-18

申请号：US17996879

申请日：2020-08-28

申请人： PULIKE BIOLOGICAL ENGINEERING, INC.

发明人： Kegong Tian ,  Wenqiang Pang ,  Yan Xiao ,  Xuke Zhang

IPC分类号： A61K39/135 ,  C12N15/86

CPC分类号： A61K39/135 ,  C12N15/86 ,  A61K2039/5258

摘要： The present disclosure provides a type A foot-and-mouth disease virus-like particle antigen assembled by VP2, VP3 and VP1 antigen proteins of an epidemic strain of type A foot-and-mouth disease virus. The type A foot-and-mouth disease virus VP2 antigen protein is encoded by a nucleotide sequence shown in SEQ ID No. 1 or its degenerate sequence, the type A foot-and-mouth disease virus VP3 antigen protein is encoded by a nucleotide sequence shown in SEQ ID No. 2 or its degenerate sequence, and the type A foot-and-mouth disease virus VP1 antigen protein is encoded by a nucleotide sequence shown in SEQ ID No. 3 or its degenerate sequence.

公开(公告)号：US20230048677A1

公开(公告)日：2023-02-16

申请号：US17758182

申请日：2021-03-09

申请人： PULIKE BIOLOGICAL ENGINEERING, INC.

发明人： Kegong Tian ,  Wenqiang Pang ,  Xuke Zhang

IPC分类号： A61K39/12 ,  C07K14/005 ,  A61P31/20

摘要： Provided is an African swine fever virus chimeric protein. The chimeric protein comprises: (1) an African swine fever virus p72 domain I; (2) an African swine fever virus p72 domain II; (3) an African swine fever virus p72 domain III; and (4) an African swine fever virus antigenic protein. By using African swine fever virus p72 protein as a skeleton, the chimeric protein provided in the present invention will exhibit antigenic epitopes of African swine fever virus antigenic proteins p54, p30, CD2v, and p12, achieve a good immune effect, and can produce significant humoral and cell-mediated immune response.

公开(公告)号：US09795667B2

公开(公告)日：2017-10-24

申请号：US15030345

申请日：2015-07-06

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Feifei Tan ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： C12N7/04 ,  A61K39/00 ,  A61K39/245 ,  C12N7/08 ,  C12N7/00 ,  A61K39/12

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N7/08 ,  C12N2710/16734 ,  C12N2710/16764

摘要： This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.

公开(公告)号：US09765105B2

公开(公告)日：2017-09-19

申请号：US14902129

申请日：2013-11-01

申请人： Pulike Biological Engineering, Inc.

发明人： Xingjin Liu ,  Xuke Zhang

IPC分类号： C07H17/08 ,  C07H17/00 ,  A61K31/7052 ,  A61K31/706

CPC分类号： C07H17/00 ,  A61K31/7052 ,  A61K31/706 ,  C07H17/08

摘要： The present disclosure relates to a macrolide compound as shown by formula I and pharmaceutically acceptable salt thereof. The compound of the present disclosure is an antibacterial agent, and can be used to treat various bacterial and protozoal infections. The present disclosure further relates to the preparation method of the compound and a pharmaceutical composition thereof.