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    Method using keyhole limpet hemocyanin composition with enhanced immunogenic activity
    3.
    发明授权
    Method using keyhole limpet hemocyanin composition with enhanced immunogenic activity 失效
    使用具有增强的免疫原性活性的匙孔血蓝蛋白组合物的方法

    公开(公告)号：US5855919A

    公开(公告)日：1999-01-05

    申请号：US837498

    申请日：1997-03-26

    申请人： Ray F. Ebert ,  Richard D. Swerdlow

    发明人： Ray F. Ebert ,  Richard D. Swerdlow

    IPC分类号： A61K38/00 ,  A61P35/00 ,  C07K14/435 ,  A61K35/56

    CPC分类号： C07K14/435 ,  A61K38/00

    摘要： The present intention is directed to a stabilized keyhole limpet hemocyanin (KLH) composition in which (i) its intact non-degraded subunit is approximately 400,000 in molecular weight based on SDS-PAGE analysis; and (ii) are contained at least about 50% didecameric or higher KLH multimers, based on sedimentation-equilibrium and/or sedimentation-velocity ultracentrifugation analyses. The KLH composition is stabilized at 4.degree. C. by dissolving and storing it in an isotonic buffer preferably containing calcium and magnesium. It is critical that the KLH not have been frozen or lyophilized during its preparation or storage. The KLH composition demonstrates enhanced immunogenic activity, particularly enhanced anti-tumor activity, which is reduced if the KLH is frozen or lyophilized. The KLH composition of the present invention exhibits enhanced anti-tumor activity in a murine bladder tumor model and thereby represents a new and useful anti-tumor immunotherapeutic agent.

    摘要翻译： 本目的是针对稳定的匙孔血蓝蛋白（KLH）组合物，其中（i）基于SDS-PAGE分析，其完整的未降解亚基的分子量约为400,000; 和（ii）基于沉降 - 平衡和/或沉降速度超速离心分析，含有至少约50％的多孔或更高的KLH多聚体。 通过将KLH组合物溶解并储存在优选含有钙和镁的等渗缓冲液中，将其稳定在4℃。 在制备或储存期间，KLH不被冷冻或冻干是至关重要的。 KLH组合物表现出增强的免疫原性活性，特别是增强的抗肿瘤活性，如果KLH被冷冻或冻干，则其降低。 本发明的KLH组合物在鼠膀胱肿瘤模型中表现出增强的抗肿瘤活性，因此代表了新的和有用的抗肿瘤免疫治疗剂。

    Method for treating bladder cancer with a keyhole limpet hemocyanin composition having enhanced anti-tumor activity
    6.
    发明授权
    Method for treating bladder cancer with a keyhole limpet hemocyanin composition having enhanced anti-tumor activity 失效
    用具有增强的抗肿瘤活性的匙孔血蓝蛋白组合物治疗膀胱癌的方法

    公开(公告)号：US5981476A

    公开(公告)日：1999-11-09

    申请号：US9121

    申请日：1998-01-20

    申请人： Ray F. Ebert ,  Richard D. Swerdlow

    发明人： Ray F. Ebert ,  Richard D. Swerdlow

    IPC分类号： A61K38/00 ,  A61P35/00 ,  C07K14/435 ,  A61K38/16

    CPC分类号： C07K14/435 ,  A61K38/00

    摘要： The present invention is directed to a stabilized keyhole limpet hemocyanin (KLH) composition in which (i) its intact non-degraded subunit is approximately 400,000 in molecular weight based on SDS-PAGE analysis; and (ii) are contained at least about 50% didecameric or higher KLH multimers, based on sedimentation-equilibrium and/or sedimentation-velocity ultracentrifugation analyses. The KLH composition is stabilized at 4.degree. C. by dissolving and storing it in an isotonic buffer preferably containing calcium and magnesium. It is critical that the KLH not have been frozen or lyophilized during its preparation or storage. The KLH composition demonstrates enhanced immunogenic activity, particularly enhanced anti-tumor activity, which is reduced if the KLH is frozen or lyophilized. The KLH composition of the present invention exhibits enhanced anti-tumor activity in a murine bladder tumor model and thereby represents a new and useful anti-tumor immunotherapeutic agent.

    摘要翻译： 本发明涉及稳定的匙孔血蓝蛋白（KLH）组合物，其中（i）基于SDS-PAGE分析，其完整的未降解亚基的分子量约为400,000; 和（ii）基于沉降 - 平衡和/或沉降速度超速离心分析，含有至少约50％的多孔或更高的KLH多聚体。 通过将KLH组合物溶解并储存在优选含有钙和镁的等渗缓冲液中，将其稳定在4℃。 在制备或储存期间，KLH不被冷冻或冻干是至关重要的。 KLH组合物表现出增强的免疫原性活性，特别是增强的抗肿瘤活性，如果KLH被冷冻或冻干，则其降低。 本发明的KLH组合物在鼠膀胱肿瘤模型中表现出增强的抗肿瘤活性，因此代表了新的和有用的抗肿瘤免疫治疗剂。