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    SYSTEMS AND METHODS FOR DETECTION OF GENETIC STRUCTURAL VARIATION USING INTEGRATED ELECTROPHORETIC DNA PURIFICATION
    2.
    发明申请
    SYSTEMS AND METHODS FOR DETECTION OF GENETIC STRUCTURAL VARIATION USING INTEGRATED ELECTROPHORETIC DNA PURIFICATION 有权

    公开(公告)号：US20210088473A1

    公开(公告)日：2021-03-25

    申请号：US16603573

    申请日：2018-04-06

    申请人： Sage Science, Inc.

    发明人： T. Christian BOLES

    IPC分类号： G01N27/447 ,  G01N27/453 ,  C12Q1/686 ,  C12Q1/6806

    摘要： An electrophoresis cassette may include sample well(s), gel column(s) containing a separation gel, and elution modules arranged adjacent the gel column(s). A sample may be provided to the electrophoresis cassette and high-molecular weight DNA may be isolated from the sample. Single-copy DNA sequences may be cleaved on both sides of a repeat region of the DNA sequences to produce a cleaved sample, which then may be fractionated using gel electrophoresis. DNA fractions may be isolated from consecutive sections of the separation gel and subjected to PCR assays to detect single-copy sequences within the DNA fraction, said single-copy sequence containing repeat expansion sequences. The subjected DNA fractions may be electroeluted into the plurality of elution modules. A size of DNA fractions having the repeat expansion sequences may be determined. It is also determined if that size is above a normal repeat size range.

    Multifunctional Electrophoresis Cassette
    4.
    发明申请
    Multifunctional Electrophoresis Cassette 审中-公开
    多功能电泳盒

    公开(公告)号：US20070284250A1

    公开(公告)日：2007-12-13

    申请号：US11685634

    申请日：2007-03-13

    申请人： Gary Magnant ,  Michael Finney ,  Todd Barbera ,  William Bowers ,  Diane Kozwich ,  Samuel Seymour ,  Robert Nelson ,  T. Boles

    发明人： Gary Magnant ,  Michael Finney ,  Todd Barbera ,  William Bowers ,  Diane Kozwich ,  Samuel Seymour ,  Robert Nelson ,  T. Boles

    IPC分类号： C07K1/26 ,  C07K1/28 ,  C25B9/08

    CPC分类号： G01N27/44739 ,  G01N27/44795

    摘要： Devices and methods are provided for more efficiently performing electrophoresis and electroblotting. A sandwich structure includes an electrophoresis gel affixed to a blotting membrane. A gel casting and/or running frame is used to hold the sandwich. A method and composition that allows separation of the gel and membrane after performing a combined electrophoresis and electroblotting operation so as to allow further operations to be performed individually upon the membrane, gel or both. A uniform electrophoretic field may be created by surrounding the sandwich structure using an insulating fluid; the insulating fluid is then swapped for a conducting fluid to allow application of an electroblotting field. An apparatus automatically manages fluid exchange and actuation of electrophoresis and electroblotting electrodes. A plurality of parallel cavities may be used to hold multiple gels or gel membrane sandwiches.

    摘要翻译： 提供了更有效地进行电泳和电印迹的装置和方法。 夹心结构包括贴在印迹膜上的电泳凝胶。 凝胶浇注和/或运行框架用于夹持夹心物。 在进行组合电泳和电印迹操作之后允许分离凝胶和膜的方法和组合物，以允许在膜，凝胶或两者上分别进行进一步的操作。 可以通过使用绝缘流体围绕夹层结构来产生均匀的电泳场; 然后将绝缘流体交换用于导电流体以允许施加电印迹场。 一种装置自动地管理电泳和电印迹电极的流体交换和致动。 可以使用多个平行的空腔来容纳多个凝胶或凝胶膜三明治。

    PREPARATIVE ELECTROPHORETIC METHOD FOR TARGETED PURIFICATION OF GENOMIC DNA FRAGMENTS
    5.
    发明公开
    PREPARATIVE ELECTROPHORETIC METHOD FOR TARGETED PURIFICATION OF GENOMIC DNA FRAGMENTS 审中-公开

    公开(公告)号：US20230227808A1

    公开(公告)日：2023-07-20

    申请号：US18149603

    申请日：2023-01-03

    申请人： Sage Science, Inc. ,  Washington University

    发明人： Robi David Mitra ,  Jeffrey MILBRANDT ,  Ezra Solomon ABRAMS ,  Todd J. BARBERA ,  T. Christian BOLES

    IPC分类号： C12N15/10 ,  G01N27/447

    CPC分类号： C12N15/101 ,  G01N27/44747

    摘要： A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase re-agents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.

    SEMI-AUTOMATED RESEARCH INSTRUMENT SYSTEM
    8.
    发明申请
    SEMI-AUTOMATED RESEARCH INSTRUMENT SYSTEM 有权

    公开(公告)号：US20210062180A1

    公开(公告)日：2021-03-04

    申请号：US16959989

    申请日：2019-01-04

    申请人： Sage Science, Inc.

    发明人： Ezra ABRAMS ,  Todd J. BARBERA ,  Chris BOLES

    IPC分类号： C12N15/10 ,  G01N27/447 ,  B01L3/00

    摘要： A cassette for retaining molecules during electrophoresis has a housing with a lane configured therein. The lane has a first elongate edge and a second elongate edge, and an elution module is configured to be received in the late to divide the lane into a first chamber and a second chamber. A first buffer reservoir is positioned adjacent the first elongate edge, and a second buffer reservoir is positioned adjacent the second elongate edge. The first side of the elution module facing the first chamber comprises a porous sterile filtration membrane. The second side of the elution module facing the second chamber comprises an ultrafiltration membrane that has a pore size to retain molecules during electrophoresis.