DNA SYNTHESIS USING MAGNETIC NANOPARTICLES

    公开(公告)号:US20250027129A1

    公开(公告)日:2025-01-23

    申请号:US18478642

    申请日:2023-09-29

    Abstract: A method for serial and contemporaneous synthesis of disparate deoxy-ribonucleic acid (DNA) strands in an array of wells defined within a substrate. Each well in the array of wells contains a precursor nucleotide chain. A first subset of wells of the array of wells is designated not to receive a nucleotide of a specified nucleobase type and the first subset of wells is closed. A solution of a binding reaction enzyme and nucleotides of the specified nucleobase type bound with a corresponding chemical blocker is flowed over the array of wells. Nucleotides of the specified nucleobase type are received in each of a second subset of wells of the array of wells that are open and designated to receive the nucleotide of the specified nucleobase type. Received nucleotides of the specified nucleobase type are bound with assistance of the binding reaction enzyme to corresponding precursor nucleotide chains in the second subset of wells.

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