公开(公告)号：US20240268415A1

公开(公告)日：2024-08-15

申请号：US18644506

申请日：2024-04-24

Applicant: Upside Foods, Inc.

Inventor： Mark Peterson

IPC: A23J3/22 ,  A23J3/06 ,  A23J3/26 ,  A23J3/28

CPC classification number: A23J3/227 ,  A23J3/06 ,  A23J3/26 ,  A23J3/28

Abstract: This disclosure describes methods for forming cell-based-meat products that mimic cuts of slaughtered meat. Generally, the disclosed method comprises forming a cell mass into primary structures, including fibers and/or sheets. A toughening agent is applied to the exterior surfaces of the primary structures, and the primary structures are arranged to mimic structures in a target slaughtered meat. The toughening agent creates a textural contrast that, during consumption, is like the textural contrast experienced when biting through bundles of meat fibers in slaughtered meat.

公开(公告)号：US12037569B2

公开(公告)日：2024-07-16

申请号：US18394522

申请日：2023-12-22

Applicant: Upside Foods, Inc.

Inventor： Matthew Leung ,  Konrad Müller-Auffermann

IPC: C12M3/00 ,  A22C17/00 ,  A23J3/04 ,  C12N5/077

CPC classification number: C12M21/08 ,  A22C17/0006 ,  A23J3/04 ,  C12N5/0652

Abstract: This disclosure describes methods for growing cells in a controlled environment in a growing room, transporting the cells to a harvest room, and removing the cells from the controlled environment in the harvest room. The disclosed method can limit exposure of cells to contaminants by maintaining a controlled environment through cell growth and additional processing. For instance, the cells may be washed and cooled within the controlled environment. The cells are transferred to a harvest room where they are harvested and removed from the controlled environment.

公开(公告)号：US11981884B2

公开(公告)日：2024-05-14

申请号：US18047196

申请日：2022-10-17

Applicant: Upside Foods, Inc.

Inventor： Charles Knight Benton ,  Konrad Müller-Auffermann

IPC: C12M3/00 ,  A22C17/00 ,  C12M1/00 ,  C12M1/12 ,  C12M3/04 ,  A23L13/00

CPC classification number: C12M23/06 ,  A22C17/0006 ,  C12M23/58 ,  C12M25/14 ,  C12M27/10 ,  A23L13/00 ,  A23V2002/00

Abstract: The present disclosure relates to systems, apparatuses, and methods for preparing cell-based meat products (i.e., comestible meat products). In particular, a pipe-based bioreactor is disclosed having one or more substrates disposed therein. In one or more embodiments, the one or more substrates comprise a plurality of nominally spaced substrates conforming to an interior profile of an elongated enclosure of the pipe-based bioreactor. In some embodiments, multiple pipe-based bioreactors are interconnected a fluid source for preparing cell-based meat products. In addition, various methods and procedures for utilizing embodiments of pipe-based bioreactors are disclosed.

公开(公告)号：US20240124814A1

公开(公告)日：2024-04-18

申请号：US18394522

申请日：2023-12-22

Applicant: Upside Foods, Inc.

Inventor： Matthew Leung ,  Konrad Müller-Auffermann

IPC: C12M3/00 ,  A22C17/00 ,  A23J3/04 ,  C12N5/077

CPC classification number: C12M21/08 ,  A22C17/0006 ,  A23J3/04 ,  C12N5/0652

Abstract: This disclosure describes methods for growing cells in a controlled environment in a growing room, transporting the cells to a harvest room, and removing the cells from the controlled environment in the harvest room. The disclosed method can limit exposure of cells to contaminants by maintaining a controlled environment through cell growth and additional processing. For instance, the cells may be washed and cooled within the controlled environment. The cells are transferred to a harvest room where they are harvested and removed from the controlled environment.

公开(公告)号：US20240117287A1

公开(公告)日：2024-04-11

申请号：US18542048

申请日：2023-12-15

Applicant: Upside Foods, Inc.

Inventor： Konrad Mueller-Auffermann

IPC: C12M1/12 ,  C12M1/00 ,  C12M1/33

CPC classification number: C12M25/02 ,  C12M29/10 ,  C12M45/02

Abstract: Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.

公开(公告)号：US20240114921A1

公开(公告)日：2024-04-11

申请号：US18045704

申请日：2022-10-11

Applicant: Upside Foods, Inc.

Inventor： Mark Peterson

IPC: A23J3/22 ,  A23J3/06 ,  A23J3/26 ,  A23J3/28

CPC classification number: A23J3/227 ,  A23J3/06 ,  A23J3/26 ,  A23J3/28

Abstract: This disclosure describes methods for forming cell-based-meat products that mimic cuts of slaughtered meat. Generally, the disclosed method comprises forming a cell mass into primary structures, including fibers and/or sheets. A toughening agent is applied to the exterior surfaces of the primary structures, and the primary structures are arranged to mimic structures in a target slaughtered meat. The toughening agent creates a textural contrast that, during consumption, is like the textural contrast experienced when biting through bundles of meat fibers in slaughtered meat.

公开(公告)号：US20230392120A1

公开(公告)日：2023-12-07

申请号：US18331092

申请日：2023-06-07

Applicant: Upside Foods, Inc.

Inventor： Sukhdeep Singh DHADWAR ,  Rachel Michele SCHUMAKER

IPC: C12N5/077 ,  C12N15/85 ,  C12N9/12 ,  C07K14/50 ,  C07K14/65 ,  C07K14/49 ,  A23L13/00

CPC classification number: C12N5/0658 ,  C12N15/85 ,  C12N9/1241 ,  C07K14/50 ,  C07K14/65 ,  C07K14/49 ,  A23L13/00 ,  C12N2800/107

Abstract: Provided herein are methods of engineering a cell line for reduced dependence on exogenous growth factor(s). In some embodiments, the method includes introducing into a cell one or more of: a polynucleotide comprising a coding sequence of a growth factor ligand; a polynucleotide comprising a coding sequence of a growth factor receptor; or a polynucleotide comprising a coding sequence of an activated downstream growth factor target, and culturing the cells in a cultivation infrastructure.

公开(公告)号：US20230329311A1

公开(公告)日：2023-10-19

申请号：US17659807

申请日：2022-04-19

Applicant: Upside Foods, Inc.

Inventor： Antony John Wicke

IPC: A23P30/10 ,  C12N5/077 ,  A23L13/00 ,  A23L29/00

CPC classification number: A23P30/10 ,  C12N5/0658 ,  A23L13/00 ,  A23L29/06 ,  A23V2002/00

Abstract: This disclosure relates to methods of shaping a raw cell-based-meat product using vacuum sealing. The disclosed method includes preparing a cell-mass mixture by mixing a grown cell mass with a binding agent. The cell-mass mixture is added to a mold that is covered with a film and vacuum sealed. Because, in some embodiments, the mold is rigid, the cell-mass mixture conforms to the shape of the mold, such as the shape of slaughtered or harvested meat. To shape a cell-based-meat product, an apparatus may be used with a mold for sealing and conforming a cell-mass mixture.

公开(公告)号：US11760964B2

公开(公告)日：2023-09-19

申请号：US17572297

申请日：2022-01-10

Applicant: Upside Foods, Inc.

Inventor： Konrad Muller-Aufferman ,  Michaela Walker

IPC: C12M3/00 ,  C12N5/077 ,  C12M1/12 ,  C12M1/00 ,  A23L13/00 ,  A23L33/17 ,  A23L33/15 ,  A23L33/16 ,  A23L33/12

CPC classification number: C12M21/08 ,  A23L13/00 ,  A23L33/12 ,  A23L33/15 ,  A23L33/16 ,  A23L33/17 ,  C12M25/14 ,  C12M37/06 ,  C12M47/02 ,  C12N5/0658 ,  A23V2002/00 ,  C12N2533/90 ,  C12N2539/00

Abstract: A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.

公开(公告)号：US20220251550A1

公开(公告)日：2022-08-11

申请号：US17545632

申请日：2021-12-08

Applicant: Upside Foods, Inc.

Inventor： Nicholas J. GENOVESE ,  Danielle Nicole DESMET ,  Eric SCHULZE

IPC: C12N15/11 ,  C12N9/12 ,  C12N5/00 ,  A23L33/135 ,  C12N5/077 ,  C12N9/22

Abstract: A product and process for extending the replicative capacity of metazoan somatic cells using targeted genetic amendments to abrogate inhibition of cell-cycle progression during replicative senescence and derive clonal cell lines for scalable applications and industrial production of metazoan cell biomass. An insertion or deletion mutation using guide RNAs targeting the first exon of the transcript encoding each protein is created using CRISPR/Cas9. Targeted amendments result in inactivation of p15 and p16 proteins which increases the proliferative capacity of the modified cell populations relative to their unaltered parental populations. Combining these amendments with ancillary telomerase activity from a genetic construct directing expression of a telomerase protein homolog from a TERT gene, increases the replicative capacity of the modified cell populations indefinitely. One application is to manufacture skeletal muscle for dietary consumption using cells from the poultry species Gallus gallus; another is from the livestock species Bos taurus.