公开(公告)号：US08394767B2

公开(公告)日：2013-03-12

申请号：US12987876

申请日：2011-01-10

申请人： Ian M. Dickerson ,  Edward B. Brown

发明人： Ian M. Dickerson ,  Edward B. Brown

IPC分类号： A61K38/23 ,  A61K38/00

CPC分类号： G01N33/5011 ,  A01K67/0271 ,  A01K2267/0331 ,  A61K38/1709 ,  A61K38/1796 ,  A61K38/225 ,  C12N5/0693 ,  C12N2501/34 ,  C12N2503/02 ,  C12N2510/00 ,  G01N2333/585 ,  G01N2333/705

摘要： The present invention is directed to methods of treating cancer and preventing cancer metastasis in a subject by administering a modulator of CGRP receptor signaling. The present invention is further directed to methods of identifying novel compounds that inhibit CGRP receptor signaling, and reagents and animal models useful for the same.

摘要翻译： 本发明涉及通过施用CGRP受体信号传导的调节剂治疗癌症并预防癌症转移的方法。 本发明还涉及鉴定抑制CGRP受体信号传导的新化合物的方法，以及可用于其的试剂和动物模型。

公开(公告)号：US20060122104A1

公开(公告)日：2006-06-08

申请号：US10515421

申请日：2003-05-22

申请人： Sharon Presnell ,  Mohammad Heidaran ,  Perry Haaland ,  Neil Robbins ,  David Scharp ,  Paul Latta ,  Margaret Coutts ,  Catherine McIntyre

发明人： Sharon Presnell ,  Mohammad Heidaran ,  Perry Haaland ,  Neil Robbins ,  David Scharp ,  Paul Latta ,  Margaret Coutts ,  Catherine McIntyre

IPC分类号： C12N5/08 ,  A61K38/18

CPC分类号： C12N5/0676 ,  A61K35/12 ,  A61K2035/126 ,  C12N5/0037 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/235 ,  C12N2501/315 ,  C12N2501/335 ,  C12N2501/34 ,  C12N2501/345 ,  C12N2501/35 ,  C12N2501/37 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/41 ,  C12N2501/83 ,  C12N2501/85 ,  C12N2501/998 ,  C12N2506/22 ,  Y02A50/471

摘要： This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in a cell culture system comprising a cell culture medium and a cell attachment surface, under conditions wherein the acinar cells undergo a 3-4 fold expansion together with transdifferentiation into a modified cell phenotype (IP cells) showing characteristics of acinar cells and liver cells. The invention also relates to a method for transforming these IP cells to insulin-producing cells in vitro, comprising culturing the cells in a novel, defined medium. Also disclosed are suitable culture media for performing these methods, isolated cells having the phenotype of IP cells and/or produced by these methods, and kits for performing the methods.

摘要翻译： 本发明涉及例如扩展哺乳动物腺泡细胞的方法，包括在包括细胞培养基和细胞附着表面的细胞培养系统中培养细胞，其中腺泡细胞与 转化为显示腺泡细胞和肝细胞特征的修饰的细胞表型（IP细胞）。 本发明还涉及在体外将这些IP细胞转化成胰岛素生成细胞的方法，包括在新颖的限定培养基中培养细胞。 还公开了用于进行这些方法的合适的培养基，具有IP细胞表型和/或通过这些方法产生的分离的细胞，以及用于进行该方法的试剂盒。

公开(公告)号：US20080317720A1

公开(公告)日：2008-12-25

申请号：US11911466

申请日：2006-04-14

申请人： Noritoshi Nagaya ,  Kunio Miyatake ,  Kenji Kangawa ,  Masaharu Kataoka

发明人： Noritoshi Nagaya ,  Kunio Miyatake ,  Kenji Kangawa ,  Masaharu Kataoka

IPC分类号： A61K35/12 ,  C12N5/06 ,  A61P9/00

CPC分类号： A61L27/3843 ,  A61K35/12 ,  A61L27/3804 ,  C12N5/0667 ,  C12N2501/165 ,  C12N2501/34 ,  C12N2533/90

摘要： An objective of the present invention is to provide methods for producing progenitor cells which show a high proliferation ability ex vivo and promote neovascularization or myocardium regeneration, by culturing pluripotent cells in a medium containing adrenomedullin (AM) and/or vascular endothelial growth factor (VEGF). Moreover, another objective of the present invention is to provide the progenitor cells and uses thereof. In order to solve the above problems, the inventors of the present application have successfully developed a novel culture technique for producing progenitor cells from adipose tissues more efficiently compared to the conventional techniques, by using adrenomedullin (AM) and/or vascular endothelial growth factor (VEGF). The progenitor cells produced by the present inventors are specialized cells having a potent proliferation ability, a revascularization ability, and a myocardium regeneration ability, and have the property of not differentiating into adipocytes even if subjected to differentiation induction into adipocytes. Therefore, the progenitor cells of the present invention can be effective pharmaceutical agents for intractable diseases in the brain, lung, liver, kidney and the like.

摘要翻译： 本发明的目的是提供通过在含有肾上腺髓质素（AM）和/或血管内皮生长因子（VEGF）的培养基中培养多能细胞来产生离体显示高增殖能力并促进新生血管形成或心肌再生的祖细胞的方法 ）。 此外，本发明的另一个目的是提供祖细胞及其用途。 为了解决上述问题，本申请的发明人通过使用肾上腺髓质素（AM）和/或血管内皮生长因子（AM）和/或血管内皮生长因子（AM），成功地开发了一种新型的培养技术，用于与常规技术相比更有效地从脂肪组织产生祖细胞 VEGF）。 由本发明人生产的祖细胞是具有有效增殖能力，血运重建能力和心肌再生能力的特殊细胞，即使进行脂肪细胞分化诱导，也具有不分化成脂肪细胞的性质。 因此，本发明的祖细胞可以是脑，肺，肝，肾等中难治性疾病的有效药剂。

公开(公告)号：US06869926B1

公开(公告)日：2005-03-22

申请号：US09509306

申请日：1998-09-25

申请人： Ian Reginald Reid ,  Jillian Cornish

发明人： Ian Reginald Reid ,  Jillian Cornish

IPC分类号： A61K45/00 ,  A61K38/00 ,  A61K38/22 ,  A61P19/08 ,  A61P43/00 ,  C07K14/575 ,  C12N5/077 ,  A61K38/16

CPC分类号： C12N5/0655 ,  A61K38/00 ,  C07K14/575 ,  C12N2500/38 ,  C12N2501/34 ,  C12N2501/998 ,  Y10S530/84

摘要： The present invention relates to new therapeutic use of amylin as agent which stimulates chondrocyte proliferation and which therefore have utility in the treatment of cartilage disorders and/or cartilage mediated bone growth.

摘要翻译： 本发明涉及胰岛淀粉样多肽作为刺激软骨细胞增殖的药物的新治疗用途，因此其可用于治疗软骨疾病和/或软骨介导的骨生长。

公开(公告)号：US20060275900A1

公开(公告)日：2006-12-07

申请号：US11410954

申请日：2006-04-26

申请人： Sharon Presnell ,  Mohammad Heidaran ,  Perry Haaland ,  David Scharp ,  Paul Latta ,  Margaret Coutts ,  Catherine McIntyre

发明人： Sharon Presnell ,  Mohammad Heidaran ,  Perry Haaland ,  David Scharp ,  Paul Latta ,  Margaret Coutts ,  Catherine McIntyre

IPC分类号： C12N5/08 ,  A61K38/17

CPC分类号： C12N5/0676 ,  A61K35/12 ,  A61K2035/126 ,  C12N5/0037 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/235 ,  C12N2501/315 ,  C12N2501/335 ,  C12N2501/34 ,  C12N2501/345 ,  C12N2501/35 ,  C12N2501/37 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/41 ,  C12N2501/83 ,  C12N2501/85 ,  C12N2501/998 ,  C12N2506/22 ,  Y02A50/471

摘要： This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in a cell culture system comprising a cell culture medium and a cell attachment surface, under conditions wherein the acinar cells undergo a 3-4 fold expansion together with transdifferentiation into a modified cell phenotype (IP cells) showing characteristics of acinar cells and liver cells. The invention also relates to a method for transforming these IP cells to insulin-producing cells in vitro, comprising culturing the cells in a novel, defined medium. Also disclosed are suitable culture media for performing these methods, isolated cells having the phenotype of IP cells and/or produced by these methods, and kits for performing the methods.

公开(公告)号：US20040259244A1

公开(公告)日：2004-12-23

申请号：US10800813

申请日：2004-03-15

发明人： David William Scharp ,  Paul Presley Latta ,  Margaret Coutts ,  Catherine Anne McIntyre ,  Sharon C. Presnell ,  Mohammad A. Heidaran ,  Perry D. Haaland

IPC分类号： C12N005/08

CPC分类号： C12N5/0676 ,  A61K35/12 ,  A61K2035/126 ,  C12N5/0037 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/235 ,  C12N2501/315 ,  C12N2501/335 ,  C12N2501/34 ,  C12N2501/345 ,  C12N2501/35 ,  C12N2501/37 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/41 ,  C12N2501/83 ,  C12N2501/85 ,  C12N2501/998 ,  C12N2506/22 ,  Y02A50/471

摘要： A method of converting differentiated non-hormone producing pancreatic cells into differentiated hormone producing cells is disclosed. The method comprises two steps: first, culturing cells under conditions which convert differentiated non-hormone producing cells into stem cells; and second, culturing stem cells under conditions which provide for differentiating stem cells into hormone-producing cells. The invention defines growth and differentiation factors that are presented to the stem cells to result in their differentiation into hormone-producing cells, especially insulin-producing cells. The invention provides a new source of large quantities of hormone producing cells such as insulin-producing cells that are riot currently available for therapeutic uses such as the treatment of diabetes.

摘要翻译： 公开了将分化的非激素产生胰腺细胞转化成分化的激素产生细胞的方法。 该方法包括两个步骤：首先在将分化的非激素产生细胞转化为干细胞的条件下培养细胞; 其次，在将干细胞分化成激素产生细胞的条件下培养干细胞。 本发明定义了向干细胞提供的生长和分化因子，以导致其分化成产生激素的细胞，特别是产生胰岛素的细胞。 本发明提供了大量激素产生细胞的新来源，例如目前可用于诸如治疗糖尿病的治疗用途的疾病的胰岛素产生细胞。

公开(公告)号：US20040127406A1

公开(公告)日：2004-07-01

申请号：US10443732

申请日：2003-05-22

发明人： Sharon C. Presnell ,  Mohammad Heidaran ,  Perry Haaland

IPC分类号： A61K038/17 ,  A61K038/16 ,  C12N005/08

CPC分类号： C12N5/0676 ,  A61K35/12 ,  A61K2035/126 ,  C12N5/0037 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/235 ,  C12N2501/315 ,  C12N2501/335 ,  C12N2501/34 ,  C12N2501/345 ,  C12N2501/35 ,  C12N2501/37 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/41 ,  C12N2501/83 ,  C12N2501/85 ,  C12N2501/998 ,  C12N2506/22 ,  Y02A50/471

摘要： This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in a cell culture system comprising a cell culture medium and a cell attachment surface, under conditions wherein the acinar cells undergo a 3-4 fold expansion together with transdifferentiation into a modified cell phenotype (IP cells) showing characteristics of acinar cells and liver cells. The invention also relates to a method for transforming these IP cells to insulin-producing cells in vitro, comprising culturing the cells in a novel, defined medium. Also disclosed are suitable culture media for performing these methods, isolated cells having the phenotype of IP cells and/or produced by these methods, and kits for performing the methods.

摘要翻译： 本发明涉及例如扩展哺乳动物腺泡细胞的方法，包括在包括细胞培养基和细胞附着表面的细胞培养系统中培养细胞，其中腺泡细胞与 转化为显示腺泡细胞和肝细胞特征的修饰的细胞表型（IP细胞）。 本发明还涉及在体外将这些IP细胞转化成胰岛素生成细胞的方法，包括在新颖的限定培养基中培养细胞。 还公开了用于进行这些方法的合适的培养基，具有IP细胞表型和/或通过这些方法产生的分离的细胞，以及用于进行该方法的试剂盒。

公开(公告)号：US20110189205A1

公开(公告)日：2011-08-04

申请号：US12987876

申请日：2011-01-10

申请人： Ian M. Dickerson ,  Edward B. Brown

发明人： Ian M. Dickerson ,  Edward B. Brown

IPC分类号： A61K39/395 ,  A61K38/17 ,  A61K31/713 ,  C07K14/585 ,  A61P35/00 ,  C12N5/02 ,  G01N33/53

CPC分类号： G01N33/5011 ,  A01K67/0271 ,  A01K2267/0331 ,  A61K38/1709 ,  A61K38/1796 ,  A61K38/225 ,  C12N5/0693 ,  C12N2501/34 ,  C12N2503/02 ,  C12N2510/00 ,  G01N2333/585 ,  G01N2333/705

摘要： The present invention is directed to methods of treating cancer and preventing cancer metastasis in a subject by administering a modulator of CGRP receptor signaling. The present invention is further directed to methods of identifying novel compounds that inhibit CGRP receptor signaling, and reagents and animal models useful for the same.

摘要翻译： 本发明涉及通过施用CGRP受体信号传导的调节剂治疗癌症并预防癌症转移的方法。 本发明还涉及鉴定抑制CGRP受体信号传导的新化合物的方法，以及可用于其的试剂和动物模型。

公开(公告)号：US07208469B2

公开(公告)日：2007-04-24

申请号：US11086628

申请日：2005-03-21

申请人： Ian Reginald Reid ,  Jillian Cornish

发明人： Ian Reginald Reid ,  Jillian Cornish

IPC分类号： A61K38/22 ,  A61K35/32 ,  A61K38/00

CPC分类号： C12N5/0655 ,  A61K38/00 ,  C07K14/575 ,  C12N2500/38 ,  C12N2501/34 ,  C12N2501/998 ,  Y10S530/84

摘要： We claim a method of treating a patient to stimulate chondrocyte proliferation comprising administering to said patient an adrenomedullin analog that is adrenomedullin-(27–52) wherein said patient is suffering from cartilage disorders, and a method of stimulating chondrocyte proliferation comprising administering to chondrocyte cells adrenomedullin-(27–52).

摘要翻译： 我们提出一种治疗患者以刺激软骨细胞增殖的方法，包括向所述患者施用肾上腺髓质素类似物，其是所述患者患有软骨疾病的肾上腺髓质素（27-52），以及刺激软骨细胞增殖的方法，包括施用软骨细胞 肾上腺髓质素（27-52）。

公开(公告)号：US20050171007A1

公开(公告)日：2005-08-04

申请号：US11086628

申请日：2005-03-21

申请人： Ian Reid ,  Jillian Cornish

发明人： Ian Reid ,  Jillian Cornish

IPC分类号： A61K45/00 ,  A61K38/00 ,  A61K38/22 ,  A61P19/08 ,  A61P43/00 ,  C07K14/575 ,  C12N5/077 ,  A61K38/17

CPC分类号： C12N5/0655 ,  A61K38/00 ,  C07K14/575 ,  C12N2500/38 ,  C12N2501/34 ,  C12N2501/998 ,  Y10S530/84

摘要： This invention is directed to new therapeutic uses which involve the stimulation of chondrocyte prloliferation. More particularly, it is directed to the use of amylin and adrenomedullin as agents which stimulate chrondrocyte proliferation and which therefore have utility in the treatment of cartilage disorders and/or cartilage mediated bone growth.

摘要翻译： 本发明涉及涉及刺激软骨细胞增殖的新的治疗用途。 更具体地，涉及使用胰岛淀粉样多肽和肾上腺髓质素作为刺激软骨细胞增殖的药剂，因此其可用于治疗软骨疾病和/或软骨介导的骨生长。