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1.发明申请公开(公告)号：US20080038717A1
公开(公告)日：2008-02-14
申请号：US11761180
申请日：2007-06-11
申请人： Lisa Garrard , Dennis Henner , Steven Bass , Ronald Greene , Henry Lowman , James Wells , David Matthews
发明人： Lisa Garrard , Dennis Henner , Steven Bass , Ronald Greene , Henry Lowman , James Wells , David Matthews
CPC分类号： C40B40/02 , C07K14/005 , C07K14/575 , C07K14/61 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/75 , C12N15/1037 , C12N15/62 , C12N2750/00011 , C12N2795/14022 , C12N2795/14122 , G01N33/6845 , G01N33/74 , G01N2333/61 , Y10S436/802 , Y10S930/12
摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
摘要翻译： 提供了选择新型蛋白质如生长激素和具有改变其各自受体分子的结合特性的抗体片段变体的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。
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2.发明授权Enrichment method for variant proteins having altered binding properties, M13 phagemids, and growth hormone variants 失效具有改变的结合特性的变体蛋白质的富集方法，M13噬菌粒和生长激素变体
公开(公告)号：US5750373A
公开(公告)日：1998-05-12
申请号：US50058
申请日：1993-04-30
申请人： Lisa J. Garrard , Dennis J. Henner , Steven Bass , Ronald Greene , Henry B. Lowman , James A. Wells , David J. Matthews
发明人： Lisa J. Garrard , Dennis J. Henner , Steven Bass , Ronald Greene , Henry B. Lowman , James A. Wells , David J. Matthews
IPC分类号： A61K47/48 , C07K14/01 , C07K14/47 , C07K14/575 , C07K14/61 , C07K19/00 , C12N5/10 , C12N15/10 , C12N15/11 , C12N15/62 , C12N15/85 , C12Q1/68 , C40B40/02 , G01N33/554 , G01N33/566 , G01N33/74 , C12P21/06 , C12N15/63
CPC分类号： C40B40/02 , C07K14/005 , C07K14/575 , C07K14/61 , C12N15/1037 , C12N15/62 , G01N33/6845 , G01N33/74 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/75 , C12N2750/00011 , C12N2795/14022 , C12N2795/14122 , G01N2333/61 , Y10S436/802 , Y10S930/12
摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
摘要翻译： PCT No.PCT / US91 / 09133 Sec。 371日期：1993年04月30日 102（e）日期1993年4月30日PCT 1991年12月3日提交了一种选择具有改变其各自受体分子结合特性的生长激素和抗体片段变体的新蛋白质的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。
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3.发明授权
公开(公告)号：US6040136A
公开(公告)日：2000-03-21
申请号：US923854
申请日：1997-09-03
申请人： Lisa J. Garrard , Dennis J. Henner , Steven Bass , Ronald Greene , Henry B. Lowman , James A. Wells , David J. Matthews
发明人： Lisa J. Garrard , Dennis J. Henner , Steven Bass , Ronald Greene , Henry B. Lowman , James A. Wells , David J. Matthews
IPC分类号： A61K47/48 , C07K14/01 , C07K14/47 , C07K14/575 , C07K14/61 , C07K19/00 , C12N5/10 , C12N15/10 , C12N15/11 , C12N15/62 , C12N15/85 , C12Q1/68 , C40B40/02 , G01N33/554 , G01N33/566 , G01N33/74 , C12Q1/70 , C07H17/00 , C07K14/00 , C12P21/06
CPC分类号： C40B40/02 , C07K14/005 , C07K14/575 , C07K14/61 , C12N15/1037 , C12N15/62 , G01N33/6845 , G01N33/74 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/75 , C12N2750/00011 , C12N2795/14022 , C12N2795/14122 , G01N2333/61 , Y10S436/802 , Y10S930/12
摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
摘要翻译： 提供了选择新型蛋白质如生长激素和具有改变其各自受体分子的结合特性的抗体片段变体的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。
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公开(公告)号：US5834598A
公开(公告)日：1998-11-10
申请号：US463667
申请日：1995-06-05
申请人： Henry B. Lowman , James A. Wells
发明人： Henry B. Lowman , James A. Wells
IPC分类号： A61K47/48 , C07K14/01 , C07K14/47 , C07K14/575 , C07K14/61 , C07K19/00 , C12N5/10 , C12N15/10 , C12N15/11 , C12N15/62 , C12N15/85 , C12Q1/68 , C40B40/02 , G01N33/554 , G01N33/566 , G01N33/74 , A61K38/27
CPC分类号： C40B40/02 , C07K14/005 , C07K14/575 , C07K14/61 , C12N15/1037 , C12N15/62 , G01N33/6845 , G01N33/74 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/75 , C12N2750/00011 , C12N2795/14022 , C12N2795/14122 , G01N2333/61 , Y10S436/802 , Y10S930/12
摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
摘要翻译： 提供了选择新型蛋白质如生长激素和具有改变其各自受体分子的结合特性的抗体片段变体的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。
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5.发明申请公开(公告)号：US20100035236A1
公开(公告)日：2010-02-11
申请号：US12508859
申请日：2009-07-24
申请人： Lisa J. GARRARD , Dennis J. Henner , Steven Bass , Ronald Greene , Henry B. Lowman , James A. Wells , David J. Matthews
发明人： Lisa J. GARRARD , Dennis J. Henner , Steven Bass , Ronald Greene , Henry B. Lowman , James A. Wells , David J. Matthews
CPC分类号： C40B40/02 , C07K14/005 , C07K14/575 , C07K14/61 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/75 , C12N15/1037 , C12N15/62 , C12N2750/00011 , C12N2795/14022 , C12N2795/14122 , G01N33/6845 , G01N33/74 , G01N2333/61 , Y10S436/802 , Y10S930/12
摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
摘要翻译： 提供了选择新型蛋白质如生长激素和具有改变其各自受体分子的结合特性的抗体片段变体的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。
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公开(公告)号：US20060115874A1
公开(公告)日：2006-06-01
申请号：US11199062
申请日：2005-08-08
申请人： Lisa Garrard , Dennis Henner , Steven Bass , Ronald Greene , Henry Lowman , James Wells , David Matthews
发明人： Lisa Garrard , Dennis Henner , Steven Bass , Ronald Greene , Henry Lowman , James Wells , David Matthews
CPC分类号： C40B40/02 , C07K14/005 , C07K14/575 , C07K14/61 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/75 , C12N15/1037 , C12N15/62 , C12N2750/00011 , C12N2795/14022 , C12N2795/14122 , G01N33/6845 , G01N33/74 , G01N2333/61 , Y10S436/802 , Y10S930/12
摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
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公开(公告)号：US5821047A
公开(公告)日：1998-10-13
申请号：US463587
申请日：1995-06-05
申请人： Lisa J. Garrard , Dennis J. Henner , Steven Bass , Ronald Greene , Henry B. Lowman , James A. Wells , David J. Matthews
发明人： Lisa J. Garrard , Dennis J. Henner , Steven Bass , Ronald Greene , Henry B. Lowman , James A. Wells , David J. Matthews
IPC分类号： A61K47/48 , C07K14/01 , C07K14/47 , C07K14/575 , C07K14/61 , C07K19/00 , C12N5/10 , C12N15/10 , C12N15/11 , C12N15/62 , C12N15/85 , C12Q1/68 , C40B40/02 , G01N33/554 , G01N33/566 , G01N33/74 , C12Q1/70 , C07K16/00 , C12N7/02 , C12N15/63
CPC分类号： C40B40/02 , C07K14/005 , C07K14/575 , C07K14/61 , C12N15/1037 , C12N15/62 , G01N33/6845 , G01N33/74 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/75 , C12N2750/00011 , C12N2795/14022 , C12N2795/14122 , G01N2333/61 , Y10S436/802 , Y10S930/12
摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
摘要翻译： 提供了选择新型蛋白质如生长激素和具有改变其各自受体分子的结合特性的抗体片段变体的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。
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