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10 条记录 (耗时 0.041 秒)

    Portable inducible control system
    6.
    发明授权
    Portable inducible control system 失效
    便携式诱导控制系统

    公开(公告)号：US4912046A

    公开(公告)日：1990-03-27

    申请号：US62318

    申请日：1987-06-08

    申请人： Dennis J. Henner ,  Daniel G. Yansura

    发明人： Dennis J. Henner ,  Daniel G. Yansura

    IPC分类号： C07K14/56 ,  C12N9/86 ,  C12N15/72 ,  C12N15/75 ,  C12N15/90

    CPC分类号： C07K14/56 ,  C12N15/72 ,  C12N15/75 ,  C12N15/90 ,  C12N9/86

    摘要： Shuttle vector systems are provided for the regulated expression of subject genes in transformed hosts. A promoter/operator which does not originate with the transformed host cell controls expression of the subject gene and a repressor, which also does not originate with the host cell, regulates the promoter/operator. According to this invention, promoter/operator--repressor functionalities from one bacterium are used in other bacteria, thereby obviating the need to locate homologous expression regulatory sequences for each bacterial host.

    摘要翻译： 提供穿梭载体系统用于受体基因在转化宿主中的调节表达。 不起源于转化的宿主细胞的启动子/操纵子控制受试者基因的表达和也不是源自宿主细胞的阻遏物调节启动子/操纵子。 根据本发明，来自一种细菌的启动子/操纵子阻遏物官能团用于其它细菌，从而避免需要定位每个细菌宿主的同源表达调控序列。

    Processes and compositions for the isolation of human relaxin
    7.
    发明授权
    Processes and compositions for the isolation of human relaxin 失效
    用于分离人类松弛素的方法和组合物

    公开(公告)号：US5464756A

    公开(公告)日：1995-11-07

    申请号：US908766

    申请日：1992-07-01

    申请人： Dennis J. Henner ,  Richard L. Vandlen ,  James A. Wilkins ,  Daniel G. Yansura

    发明人： Dennis J. Henner ,  Richard L. Vandlen ,  James A. Wilkins ,  Daniel G. Yansura

    IPC分类号： A61K38/00 ,  A61P13/02 ,  A61P15/00 ,  C07H21/04 ,  C07K1/113 ,  C07K1/12 ,  C07K14/00 ,  C07K14/575 ,  C07K14/64 ,  C12N1/21 ,  C12N15/09 ,  C12N15/12 ,  C12N15/16 ,  C12P21/00 ,  C12P21/02 ,  C12P21/06 ,  C12R1/19 ,  C12N15/70

    CPC分类号： C07K14/64 ,  Y10S435/849 ,  Y10S930/31

    摘要： A process is provided for cleaving a polypeptide into at least two polypeptide components comprising treating a reduced, free-cysteine form of the polypeptide with a cleaving agent under conditions for cleaving the polypeptide at a desired junction between the polypeptide cleavage products. More preferably, the process for cleaving comprises culturing cells containing DNA encoding said polypeptide, wherein at least one Asp codon is present in said DNA at a desired junction between the components to be cleaved from each other, said culturing resulting in expression of the DNA to produce the polypeptide in the host cell culture; and treating a reduced, free-cysteine form of the polypeptide with dilute acid under conditions for cleaving the polypeptide at the Asp junction. In particular embodiments, a DNA sequence is provided that encodes a relaxin precursor and includes codons encoding aspartic acid-containing linkers at novel positions within the precursor, allowing the ready cleavage of relaxin A peptides by treatment with dilute acid.

    摘要翻译： 提供了一种用于将多肽切割成至少两种多肽组分的方法，包括在多肽切割产物之间的所需连接处切割多肽的条件下用切割剂处理还原的游离半胱氨酸形式的多肽。 更优选地，所述切割方法包括培养含有编码所述多肽的DNA的细胞，其中至少一个Asp密码子存在于所述DNA中在待切割的组分之间的所需连接处，所述培养导致DNA的表达 在宿主细胞培养物中产生多肽; 以及在Asp连接处切割多肽的条件下用稀酸处理还原的游离半胱氨酸形式的多肽。 在具体实施方案中，提供了编码松弛素前体的DNA序列，并且包括在前体内的新位置编码含有天冬氨酸的接头的密码子，从而允许通过用稀酸处理来快速切割松弛素A肽。

    Chimpanzee erythropoietin (CHEPO) polypeptides and nucleic acids encoding the same
    8.
    发明授权
    Chimpanzee erythropoietin (CHEPO) polypeptides and nucleic acids encoding the same 有权
    黑猩猩促红细胞生成素（CHEPO）多肽和编码其的核酸

    公开(公告)号：US06831060B2

    公开(公告)日：2004-12-14

    申请号：US09813775

    申请日：2001-03-20

    申请人： Frederic DeSauvage ,  Dennis J. Henner

    发明人： Frederic DeSauvage ,  Dennis J. Henner

    IPC分类号： A61K3816

    CPC分类号： C07K14/505 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00

    摘要： The present invention is directed to novel chimpanzee erythropoietin polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, and antibodies which bind to the polypeptides of the present invention.

    摘要翻译： 本发明涉及新型黑猩猩红细胞生成素多肽和编码这些多肽的核酸分子。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明多肽的嵌合多肽分子，以及结合本发明多肽的抗体。

    Chimpanzee erythropoietin (CHEPO) polypeptides and nucleic acids encoding the same
    9.
    发明授权
    Chimpanzee erythropoietin (CHEPO) polypeptides and nucleic acids encoding the same 有权
    黑猩猩促红细胞生成素（CHEPO）多肽和编码其的核酸

    公开(公告)号：US06555343B1

    公开(公告)日：2003-04-29

    申请号：US09552265

    申请日：2000-04-19

    申请人： Frederic DeSauvage ,  Dennis J. Henner

    发明人： Frederic DeSauvage ,  Dennis J. Henner

    IPC分类号： C12N1509

    CPC分类号： C07K14/505 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00 ,  Y10S977/773 ,  Y10S977/907

    摘要： The present invention is directed to novel chimpanzee erythropoietin polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

    摘要翻译： 本发明涉及新型黑猩猩红细胞生成素多肽和编码这些多肽的核酸分子。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。

    Protease deficient bacillus
    10.
    发明授权
    Protease deficient bacillus 失效
    蛋白酶缺陷型杆菌

    公开(公告)号：US5264366A

    公开(公告)日：1993-11-23

    申请号：US797577

    申请日：1991-11-25

    申请人： Eugenio Ferrari ,  Dennis J. Henner ,  Mark L. Stahl

    发明人： Eugenio Ferrari ,  Dennis J. Henner ,  Mark L. Stahl

    IPC分类号： C11D3/386 ,  C12N9/54 ,  C12N15/10 ,  C12N15/75 ,  C12N15/00 ,  C12N15/03 ,  C12N15/31

    CPC分类号： C12R1/07 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54

    摘要： There are described, normally sporulating mutant Bacillus strain(s) which produce no detectable proteolytic activity during any phase of its growth. The absence of detectable proteolytic activity is due to the deletion of one or more codons specifying the mature subtilisin protease and the mature neutral protease. Also described are methods for producing such normally sporulating, protease deficient Bacillus mutants.

    摘要翻译： 描述了通常是孢子形成的突变芽孢杆菌菌株，其在其生长的任何阶段都不产生可检测的蛋白水解活性。 不存在可检测的蛋白水解活性是由于缺失一个或多个密码子，指定成熟的枯草杆菌蛋白酶和成熟的中性蛋白酶。 还描述了用于产生这种通常形成孢子的蛋白酶缺陷芽孢杆菌突变体的方法。