公开(公告)号：US20080038717A1

公开(公告)日：2008-02-14

申请号：US11761180

申请日：2007-06-11

申请人： Lisa Garrard ,  Dennis Henner ,  Steven Bass ,  Ronald Greene ,  Henry Lowman ,  James Wells ,  David Matthews

发明人： Lisa Garrard ,  Dennis Henner ,  Steven Bass ,  Ronald Greene ,  Henry Lowman ,  James Wells ,  David Matthews

IPC分类号： C12Q1/70 ,  C07K14/00 ,  C12N7/00

CPC分类号： C40B40/02 ,  C07K14/005 ,  C07K14/575 ,  C07K14/61 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/735 ,  C07K2319/75 ,  C12N15/1037 ,  C12N15/62 ,  C12N2750/00011 ,  C12N2795/14022 ,  C12N2795/14122 ,  G01N33/6845 ,  G01N33/74 ,  G01N2333/61 ,  Y10S436/802 ,  Y10S930/12

摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.

摘要翻译： 提供了选择新型蛋白质如生长激素和具有改变其各自受体分子的结合特性的抗体片段变体的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。

公开(公告)号：US20060110723A1

公开(公告)日：2006-05-25

申请号：US10014731

申请日：2001-12-11

申请人： Joseph Monforte

发明人： Joseph Monforte

IPC分类号： C12Q1/70

CPC分类号： G01N33/6842 ,  C12Q1/6809 ,  C12Q1/6844 ,  G01N33/58 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/6848 ,  Y10S435/973 ,  Y10S436/802 ,  Y10T436/24 ,  C12Q2565/531 ,  C12Q2563/131 ,  C12Q2537/143

摘要： A system is for analyzing expression levels and activity of a plurality of proteins is provided. A bio-displayed polypeptide binding component associated with a predetermined marker is used to bind the proteins of interest. The predetermined marker components are then amplified and detected in a high throughput manner.

摘要翻译： 提供了用于分析多种蛋白质的表达水平和活性的系统。 与预定标记物相关的生物显示多肽结合成分用于结合感兴趣的蛋白质。 然后以高通量方式放大和检测预定标记物组分。

公开(公告)号：US4797363A

公开(公告)日：1989-01-10

申请号：US885328

申请日：1986-07-14

申请人： Marius C. Teodorescu ,  Alexandre M. Gaspar

发明人： Marius C. Teodorescu ,  Alexandre M. Gaspar

IPC分类号： C12Q1/04 ,  C12Q1/70 ,  G01N33/53 ,  G01N33/554 ,  G01N33/569 ,  G01N33/58 ,  C12N7/00 ,  C12N7/02 ,  C12N13/00

CPC分类号： C12Q1/70 ,  C12Q1/04 ,  G01N33/53 ,  G01N33/554 ,  G01N33/56983 ,  G01N33/586 ,  Y10S436/802

摘要： Bacteriophages are employed as agents for recognition and identification of molecules and cellular materials, using their ability to recognize their bacterial host, by coating them with antibodies or by selecting them to perform in a manner analogous to antibodies. Visibility for identification is effected by incorporating a fluorescent agent, a radioisotope, a metal, an enzyme, or other staining material. The bacteriophage are prepared so as to bind to the molecular or cellular material through either the tail or head segment of the bacteriophage.

摘要翻译： 噬菌体用作识别和鉴定分子和细胞材料的试剂，使用它们识别其细菌宿主的能力，通过用抗体包被或通过选择它们以类似于抗体的方式进行。 用于鉴定的可见性通过掺入荧光剂，放射性同位素，金属，酶或其它染色材料来实现。 制备噬菌体以通过噬菌体的尾部或头部部分结合分子或细胞材料。

公开(公告)号：US06174664B1

公开(公告)日：2001-01-16

申请号：US09305594

申请日：1999-05-05

申请人： Robert Phillips Heine

发明人： Robert Phillips Heine

IPC分类号： G01N3353

CPC分类号： G01N33/68 ,  G01N2333/22 ,  G01N2333/295 ,  G01N2333/4721 ,  G01N2333/79 ,  Y10S435/962 ,  Y10S435/97 ,  Y10S435/973 ,  Y10S436/802 ,  Y10S436/805 ,  Y10S436/811

摘要： The present invention provides a relatively accurate, rapid and economical method of screening a patient for the presence of inflammatory diseases such as an intraamniotic infection, bacterial meningitis and the sexually transmitted diseases; gonorrhea, chlamydia and trichomoniasis. The method of the present invention involves measuring the concentration of neutrophil defensins HNP1-3 and the concentration of lactoferrin, found in a bodily fluid, tissue or a combination thereof, adding these two concentrations together to yield a summed total, and correlating the measured summed total to known summed totals to give an indication of whether the patient is at risk of suffering from inflammatory diseases such as an intraamniotic infection, bacterial meningitis or the sexually transmitted diseases; gonorrhea, chlamydia and trichomoniasis.

摘要翻译： 本发明提供了一种相对准确，快速和经济的筛选病人存在炎性疾病如羊膜内感染，细菌性脑膜炎和性传播疾病的方法; 淋病，衣原体和毛滴虫病。 本发明的方法包括测量嗜中性粒细胞防御素HNP1-3的浓度和在体液，组织或其组合中发现的乳铁蛋白的浓度，将这两个浓度加在一起以产生总和，并将测得的总和 总共到已知的总计总数来表示患者是否患有炎性疾病如羊膜内感染，细菌性脑膜炎或性传播疾病的风险; 淋病，衣原体和毛滴虫病。

公开(公告)号：US5750373A

公开(公告)日：1998-05-12

申请号：US50058

申请日：1993-04-30

申请人： Lisa J. Garrard ,  Dennis J. Henner ,  Steven Bass ,  Ronald Greene ,  Henry B. Lowman ,  James A. Wells ,  David J. Matthews

发明人： Lisa J. Garrard ,  Dennis J. Henner ,  Steven Bass ,  Ronald Greene ,  Henry B. Lowman ,  James A. Wells ,  David J. Matthews

IPC分类号： A61K47/48 ,  C07K14/01 ,  C07K14/47 ,  C07K14/575 ,  C07K14/61 ,  C07K19/00 ,  C12N5/10 ,  C12N15/10 ,  C12N15/11 ,  C12N15/62 ,  C12N15/85 ,  C12Q1/68 ,  C40B40/02 ,  G01N33/554 ,  G01N33/566 ,  G01N33/74 ,  C12P21/06 ,  C12N15/63

CPC分类号： C40B40/02 ,  C07K14/005 ,  C07K14/575 ,  C07K14/61 ,  C12N15/1037 ,  C12N15/62 ,  G01N33/6845 ,  G01N33/74 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/735 ,  C07K2319/75 ,  C12N2750/00011 ,  C12N2795/14022 ,  C12N2795/14122 ,  G01N2333/61 ,  Y10S436/802 ,  Y10S930/12

摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.

摘要翻译： PCT No.PCT / US91 / 09133 Sec。 371日期：1993年04月30日 102（e）日期1993年4月30日PCT 1991年12月3日提交了一种选择具有改变其各自受体分子结合特性的生长激素和抗体片段变体的新蛋白质的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。

公开(公告)号：US3717705A

公开(公告)日：1973-02-20

申请号：US3717705D

申请日：1970-01-09

申请人： SELA M ,  HAIMOVICH J

发明人： HAIMOVICH J ,  SELA M

IPC分类号： G01N33/53 ,  A61K35/66 ,  C12Q1/70 ,  G01N33/532 ,  G01N33/542 ,  G01N33/68 ,  G01N31/02 ,  G01N33/16

CPC分类号： C12Q1/70 ,  G01N33/532 ,  G01N33/542 ,  Y10S436/802 ,  Y10S436/817

摘要： THE NOVEL CONJUGATES ARE PREPARED BY USING BIFUNCTIONAL MOLECULES AS LINKING AGENTS. THE CONJUGATES WHICH SURVIVE THE COUPLING PROCESS CAN BE USED FOR DETECTING AND DETERMINING QUANTITATIVELT VERY SMALL QUANTITIES OF ANTI-PROTEIN ANTIBODIES. QUANTITIES AS LITTLE AS 0.2 TO 2.0 NANOGRAMS PER MILLILITER OF ANTISERUM CAN BE DETACTED. INSULIN-BACTERIOPHAGE CONJUGATE WAS INACTIVATED TO A SIGNIFICANT EXTENT BY SERA OF DIABETIC PATIENTS WHO HAS RECEIVED INJECTIONS OF INSULIN, WHICH WAS DILUTED A HUNDRED TO TEN-THOUSAND FOLD. THE INACTIVATION OF PROTEIN-BACTERIOPHAGE CONJUGATES BY THE CORRESPONDING ANTI-PROTEIN CAN BE INHIBITED BY THE ADDITION OF PREDETERMINED QUANTITIES OF THE SAID PROTEIN AND THIS INHIBITION CAN BE USED FOR THE DETERMINATION OF VERY SMALL QUANTITIES OF THE PROTEIN.

公开(公告)号：US20100035236A1

公开(公告)日：2010-02-11

申请号：US12508859

申请日：2009-07-24

申请人： Lisa J. GARRARD ,  Dennis J. Henner ,  Steven Bass ,  Ronald Greene ,  Henry B. Lowman ,  James A. Wells ,  David J. Matthews

发明人： Lisa J. GARRARD ,  Dennis J. Henner ,  Steven Bass ,  Ronald Greene ,  Henry B. Lowman ,  James A. Wells ,  David J. Matthews

IPC分类号： C12Q1/70 ,  C07K14/61 ,  C12N7/00

CPC分类号： C40B40/02 ,  C07K14/005 ,  C07K14/575 ,  C07K14/61 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/735 ,  C07K2319/75 ,  C12N15/1037 ,  C12N15/62 ,  C12N2750/00011 ,  C12N2795/14022 ,  C12N2795/14122 ,  G01N33/6845 ,  G01N33/74 ,  G01N2333/61 ,  Y10S436/802 ,  Y10S930/12

摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.

摘要翻译： 提供了选择新型蛋白质如生长激素和具有改变其各自受体分子的结合特性的抗体片段变体的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。

公开(公告)号：US20060115874A1

公开(公告)日：2006-06-01

申请号：US11199062

申请日：2005-08-08

申请人： Lisa Garrard ,  Dennis Henner ,  Steven Bass ,  Ronald Greene ,  Henry Lowman ,  James Wells ,  David Matthews

发明人： Lisa Garrard ,  Dennis Henner ,  Steven Bass ,  Ronald Greene ,  Henry Lowman ,  James Wells ,  David Matthews

IPC分类号： C12P21/06 ,  C12N9/64 ,  C12N9/80 ,  C07K14/16 ,  C07K14/65 ,  C07K14/705 ,  C12N15/74

CPC分类号： C40B40/02 ,  C07K14/005 ,  C07K14/575 ,  C07K14/61 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/735 ,  C07K2319/75 ,  C12N15/1037 ,  C12N15/62 ,  C12N2750/00011 ,  C12N2795/14022 ,  C12N2795/14122 ,  G01N33/6845 ,  G01N33/74 ,  G01N2333/61 ,  Y10S436/802 ,  Y10S930/12

摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.

公开(公告)号：US20040248298A1

公开(公告)日：2004-12-09

申请号：US10482235

申请日：2004-06-15

发明人： Michael Schutz ,  Renate Grassl ,  Roman Meyer ,  Sibylle Frick ,  Ingrid Robl ,  Thomas Zander ,  Stefan Miller

IPC分类号： C12N007/00 ,  C12N007/01 ,  C12N005/00 ,  C12N005/02

CPC分类号： C07K14/005 ,  C12N15/1013 ,  C12N2795/10222 ,  Y10S436/802 ,  Y10T436/10

摘要： The present invention relates to a method for the selective purification of bacterial cells and/or cell components, whereby the purification is performed by means of a solid support.

摘要翻译： 本发明涉及一种选择性纯化细菌细胞和/或细胞成分的方法，由此通过固体支持物进行纯化。

公开(公告)号：US5821047A

公开(公告)日：1998-10-13

申请号：US463587

申请日：1995-06-05

申请人： Lisa J. Garrard ,  Dennis J. Henner ,  Steven Bass ,  Ronald Greene ,  Henry B. Lowman ,  James A. Wells ,  David J. Matthews

发明人： Lisa J. Garrard ,  Dennis J. Henner ,  Steven Bass ,  Ronald Greene ,  Henry B. Lowman ,  James A. Wells ,  David J. Matthews

IPC分类号： A61K47/48 ,  C07K14/01 ,  C07K14/47 ,  C07K14/575 ,  C07K14/61 ,  C07K19/00 ,  C12N5/10 ,  C12N15/10 ,  C12N15/11 ,  C12N15/62 ,  C12N15/85 ,  C12Q1/68 ,  C40B40/02 ,  G01N33/554 ,  G01N33/566 ,  G01N33/74 ,  C12Q1/70 ,  C07K16/00 ,  C12N7/02 ,  C12N15/63

CPC分类号： C40B40/02 ,  C07K14/005 ,  C07K14/575 ,  C07K14/61 ,  C12N15/1037 ,  C12N15/62 ,  G01N33/6845 ,  G01N33/74 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/735 ,  C07K2319/75 ,  C12N2750/00011 ,  C12N2795/14022 ,  C12N2795/14122 ,  G01N2333/61 ,  Y10S436/802 ,  Y10S930/12

摘要： A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.

摘要翻译： 提供了选择新型蛋白质如生长激素和具有改变其各自受体分子的结合特性的抗体片段变体的方法。 该方法包括将编码目的蛋白质的基因融合到丝状噬菌体M13的基因III外壳蛋白的羧基末端结构域上。 基因融合突变以形成在噬菌粒粒子表面上以低量表达的结构相关融合蛋白的文库。 使用生物选择和筛选来鉴定可用作候选药物的新型配体。 公开了优选的噬菌粒表达载体和选择的人生长激素变体。