摘要:
The present invention relates to methods for preparing patchoulol, β-santalene, and sclareol in transgenic moss cells that include heterologous nucleic acid molecules encoding a polypeptide or synthase capable of using FPP or GGPP as a substrate. Methods for producing the transgenic moss cell, as well as the transgenic moss cell itself are also disclosed.
摘要:
An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a) or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.
摘要:
An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a) or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.
摘要翻译:编码萜烯合酶的分离的核酸分子,其选自:a)包含SEQ ID NO:1,SEQ ID NO:3或SEQ ID NO:5所示核苷酸序列的核酸分子; b)作为(a)的片段的核酸分子; c)包含与(a)或(b)互补的核苷酸序列的核酸分子; 和d)编码具有至少约至少约60%,65%,70%,75%,80%,85%,90%,95%,96%,97%,98的萜烯合酶的核酸分子 (a) - (c)中任何一个的%,99%同一性; 其中所述核酸分子编码萜烯合酶。
摘要:
An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a) or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.
摘要翻译:编码萜烯合酶的分离的核酸分子,其选自:a)包含SEQ ID NO:1,SEQ ID NO:3或SEQ ID NO:5所示核苷酸序列的核酸分子; b)作为(a)的片段的核酸分子; c)包含与(a)或(b)互补的核苷酸序列的核酸分子; 和d)编码具有至少约至少约60%,65%,70%,75%,80%,85%,90%,95%,96%,97%,98的萜烯合酶的核酸分子 (a) - (c)中任何一个的%,99%同一性; 其中所述核酸分子编码萜烯合酶。
摘要:
The present invention provides a method of producing β-santalene, said method comprising contacting at least one polypeptide with farnesyl pyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing β-santalene is also an object of the present invention.
摘要:
The present invention provides a method of producing β-santalene, said method comprising contacting at least one polypeptide with farnesyl pyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing β-santalene is also an object of the present invention.
摘要:
An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a)- or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.
摘要翻译:编码萜烯合酶的分离的核酸分子,其选自:a)包含SEQ ID NO:1,SEQ ID NO:3或SEQ ID NO:5所示核苷酸序列的核酸分子; b)作为(a)的片段的核酸分子; c)包含与(a) - 或(b)互补的核苷酸序列的核酸分子; 65,70%,75%,80%,85%,90%,95%,96%的萜烯合成酶的核酸分子, (a) - (c)中任一个的97%,98%,99%同一性; 其中所述核酸分子编码萜烯合酶。
摘要:
Provided are modified santalene synthase polypeptides, nucleic acid molecules encoding the modified santalene synthase polypeptides, and methods of using the modified santalene synthase polypeptides. The modified santalene synthase polypeptides include those that catalyze production of increased levels of terpenes or altered profiles thereof or both.
摘要:
A method of producing β-santalene by contacting at least one polypeptide with farnesyl pyrophosphate (FPP). This method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing the nucleic acid are also disclosed, along with a non-human host organism or a cell transformed to be used in the method of producing β-santalene.
摘要:
An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a) or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.
摘要翻译:编码萜烯合酶的分离的核酸分子,其选自:a)包含SEQ ID NO:1,SEQ ID NO:3或SEQ ID NO:5所示核苷酸序列的核酸分子; b)作为(a)的片段的核酸分子; c)包含与(a)或(b)互补的核苷酸序列的核酸分子; 和d)编码具有至少约至少约60%,65%,70%,75%,80%,85%,90%,95%,96%,97%,98的萜烯合酶的核酸分子 (a) - (c)中任何一个的%,99%同一性; 其中所述核酸分子编码萜烯合酶。