公开(公告)号：US20050214918A1

公开(公告)日：2005-09-29

申请号：US10961826

申请日：2004-10-08

Applicant: Aled Edwards ,  Akil Dharamsi ,  Masoud Vedadi ,  Matthew Kimber

Inventor： Aled Edwards ,  Akil Dharamsi ,  Masoud Vedadi ,  Matthew Kimber

IPC: C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12Q1/18 ,  C12Q1/48 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC classification number: C12N9/0008 ,  C07K2299/00 ,  C12N9/0006 ,  C12N9/1085 ,  C12N9/1241 ,  C12N9/93 ,  C12Q1/18 ,  C12Q1/48 ,  C12Y101/01158 ,  C12Y102/01011 ,  C12Y207/07023 ,  C12Y603/02004 ,  C12Y603/02009 ,  C12Y603/0201 ,  G01N2333/315 ,  G01N2333/91034 ,  G16B15/00

Abstract: The present invention relates to novel drug targets for pathogenic bacteria. Accordingly, the invention provides purified protein comprising the amino acid sequence set forth in SEQ ID NO: 4. The invention also provides biochemical and biophysical characteristics of the polypeptides of the invention.

Abstract translation: 本发明涉及病原菌的新型药物靶标。 因此，本发明提供了包含SEQ ID NO：4所示氨基酸序列的纯化蛋白质。本发明还提供本发明多肽的生物化学和生物物理学特征。

公开(公告)号：US20060183676A1

公开(公告)日：2006-08-17

申请号：US10478268

申请日：2002-05-20

Applicant: Marcus Horwitz ,  Gunter Harth ,  Paul Zamecnik ,  David Tabatadze

Inventor： Marcus Horwitz ,  Gunter Harth ,  Paul Zamecnik ,  David Tabatadze

IPC: A61K48/00

CPC classification number: C12N15/113 ,  A61K38/00 ,  C12N2310/315 ,  C12N2310/351 ,  C12N2310/3517 ,  C12Y205/01019 ,  C12Y501/01001 ,  C12Y603/01002 ,  C12Y603/02004 ,  C12Y603/02009 ,  C12Y603/0201

Abstract: Methods of inhibiting the proliferation of Mycobacterium tuberculosis comprising contacting Mycobacterium tuberculosis with an effective amount of a polynucleotide complementary to an mRNA transcript expressed by Mycobacterium tuberculosis are provided. Typical methods of the invention utilize phosphorothioate modified antisense polynucleotides (PS-ODNs) against the mRNA of M. tuberculosis genes such as glutamine synthetase, aroA, ask, groES, and the genes of the Antigen 85 complex. Optionally, the methods employ multiple antisense polynucleotides targeting different Mycobacterium tuberculosis transcripts. In preferred embodiments of the invention, the antisense polynucleotides are complementary to the 5′ regions of the Mycobacterium tuberculosis transcripts.

Abstract translation: 提供了抑制结核分枝杆菌增殖的方法，其包括使结核分枝杆菌与由结核分枝杆菌表达的mRNA转录物互补的有效量的多核苷酸接触。 本发明的典型方法利用硫代磷酸酯修饰的反义多核苷酸（PS-ODN）针对结核分枝杆菌基因的mRNA，如谷氨酰胺合成酶，aroA，ask，groES以及抗原85复合物的基因。 任选地，所述方法使用靶向不同结核分枝杆菌转录本的多个反义多核苷酸。 在本发明的优选实施方案中，反义多核苷酸与结核分枝杆菌转录本的5'区域互补。