公开(公告)号：US06555116B1

公开(公告)日：2003-04-29

申请号：US09238379

申请日：1999-01-27

申请人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihon C. Yee ,  Oscar L. Frick

发明人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihon C. Yee ,  Oscar L. Frick

IPC分类号： A61K3936

CPC分类号： A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A21D13/064 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L5/27 ,  A23L7/109 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A61K39/35 ,  A61K2035/122 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009 ,  Y10S436/904 ,  Y10T436/182

摘要： Thioredoxin, a small dithiol protein, is a specific reductant for allergenic proteins and particularly allergenic proteins present in pollen and animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by lipoic acid chemical reductant. Skin tests carried out with sensitized dogs showed that treatment of the pollens with reduced thioredoxin prior to injection eliminated or decreased the allergenicity of the pollen. Studies showed increased digestion of the pollen proteins by pepsin following reduction by thioredoxin. Pollen proteins that have been reduced by thioredoxin are effective and safe immunotherapeutic agents for decreasing or eliminating an animal's allergic reaction that would otherwise occur upon exposure to the non-reduced pollen protein.

摘要翻译： 硫氧还蛋白，一种小二硫醇蛋白，是一种特异性的还原剂，适用于过敏原蛋白，特别是花粉和动植物来源的变应原蛋白。 所有靶向蛋白含有通过硫氧还蛋白还原成巯基（SH）水平的二硫键（S-S）键。 蛋白质是过敏性活性的，在氧化（S-S）状态下消化不良。 当减少（SH状态）时，它们失去过敏原性和/或变得更易消化。 当NADPH通过NADP-硫氧还蛋白还原酶（生理条件）或硫辛酸化学还原剂被活化（还原）时，硫氧还蛋白实现了这一还原。 用致敏狗进行的皮肤试验表明，在注射前用降低的硫氧还蛋白处理花粉消除或降低了花粉的致敏性。 研究显示硫氧还蛋白还原后，通过胃蛋白酶消化花粉蛋白。 通过硫氧还蛋白还原的花粉蛋白是有效和安全的免疫治疗剂，用于减少或消除否则在暴露于未还原花粉蛋白时会发生的动物过敏反应。

公开(公告)号：US06277383B1

公开(公告)日：2001-08-21

申请号：US08410614

申请日：1995-03-24

申请人： Mohan Bir Singh ,  Robert Bruce Knox ,  Penelope Smith ,  Asil Avjioglu ,  Piyada Theerakulpisut ,  Terryn Hough ,  Cenk Suphioglu ,  Eng Kok Ong

发明人： Mohan Bir Singh ,  Robert Bruce Knox ,  Penelope Smith ,  Asil Avjioglu ,  Piyada Theerakulpisut ,  Terryn Hough ,  Cenk Suphioglu ,  Eng Kok Ong

IPC分类号： A61K3936

CPC分类号： C12N15/8289 ,  A61K38/00 ,  A61K39/00 ,  C07K14/415 ,  C07K16/16 ,  C07K2319/02 ,  G01N33/569 ,  G01N33/56961 ,  G01N33/56972 ,  G01N33/689 ,  G01N33/6893 ,  G01N2800/24

摘要： The present invention provides nucleic acid sequences coding for two ryegrass pollen allergen Lol p Ib family members, purified Lol p Ib.1 and Lol p Ib.2 proteins and fragments thereof, methods of producing recombinant Lol p Ib.1 or Lol p Ib.2 or at least one fragment thereof or derivative or homologue thereof, and methods of using the nucleic acid sequences, proteins and peptides of the invention.

摘要翻译： 本发明提供编码两种黑麦草花粉变应原Lol p Ib家族成员，纯化的Lol p Ib.1和Lol p Ib.2蛋白及其片段的核酸序列，生产重组Lol p Ib.1或Lol p Ib的方法。 2或其至少一个片段或其衍生物或同源物，以及使用本发明的核酸序列，蛋白质和肽的方法。

公开(公告)号：US06239269B1

公开(公告)日：2001-05-29

申请号：US08433288

申请日：1995-05-03

申请人： Mohan Bir Singh ,  Robert Bruce Knox ,  Penelope Smith ,  Asil Avjioglu ,  Piyada Theerakulpisut ,  Terryn Hough

发明人： Mohan Bir Singh ,  Robert Bruce Knox ,  Penelope Smith ,  Asil Avjioglu ,  Piyada Theerakulpisut ,  Terryn Hough

IPC分类号： A61K3936

CPC分类号： C07K16/16 ,  C07K14/415 ,  G01N2800/24

摘要： The present invention provides a nucleic acid sequences coding for the ryegrass pollen allergens Lol pIa and Lol pIb, purified Lol pIa and Lol pIb protein and fragments thereof, methods of producing recombinant Lol pIa and Lol pIb or at least one fragment thereof or derivative or homologue thereof, and methods of using the nucleic acid sequences, proteins and peptides of the invention.

摘要翻译： 本发明提供了编码黑麦草花粉过敏原Lol pIa和Lol pIb，纯化的Lol pIa和Lol pIb蛋白及其片段的核酸序列，产生重组Lol pIa和Lol pIb的方法或其至少一种片段或衍生物或同系物 以及使用本发明的核酸序列，蛋白质和肽的方法。