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公开(公告)号：US06841376B2
公开(公告)日：2005-01-11
申请号：US09872868
申请日：2001-05-01
申请人： Timothy Gardner , James J. Collins
发明人： Timothy Gardner , James J. Collins
CPC分类号： C12N15/635 , C12N15/63
摘要： Provided are methods and compositions for regulating gene expression in a cell. The invention provides recombinant genetic toggle switches which contain a first constitutive promoter-regulatory gene operon and a second constitutive promoter-regulatory gene operon. Expression of the regulatory gene from the first operon inhibits expression from the promoter in the second operon, and expression of the regulatory gene from the second operon inhibits expression from the promoter in the first operon. By use of the toggle switch and various switching agents it is possible to reversibly switch the expression of a gene of interest between a stable “on” state and stable “off” state or vice versa via transient exposure to a switching agent.
摘要翻译： 提供用于调节细胞中基因表达的方法和组合物。 本发明提供含有第一组成型启动子调控基因操纵子和第二组成型启动子调控基因操纵子的重组遗传切换开关。 来自第一操纵子的调节基因的表达抑制第二操纵子中启动子的表达，并且来自第二操纵子的调节基因的表达抑制来自第一操纵子中启动子的表达。 通过使用拨动开关和各种开关剂，可以通过瞬时暴露于开关剂来将感兴趣的基因的表达在稳定的“导通”状态和稳定的“断开”状态之间进行可逆的切换。
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公开(公告)号：US06828140B2
公开(公告)日：2004-12-07
申请号：US09872339
申请日：2001-06-01
申请人： Timothy Gardner , James J. Collins
发明人： Timothy Gardner , James J. Collins
IPC分类号： C12N120
CPC分类号： C12N15/635 , C12N15/63
摘要： Provided are methods and compositions for regulating gene expression in a cell. The invention provides recombinant adjustable-threshold switches which contain a first inducible promoter-regulatory gene operon and a second constitutive promoter-regulatory gene operon. A threshold amount of activating agent is required to switch the inducible promoter “on” and the constitutive promoter “off”. An adjustable-threshold switch is useful to reversibly switch the expression of a gene of interest between a stable “on” state and stable “off” state in response to an activating agent at or above a threshold concentration.
摘要翻译： 提供用于调节细胞中基因表达的方法和组合物。 本发明提供了包含第一诱导型启动子调控基因操纵子和第二组成型启动子调控基因操纵子的重组可调阈值开关。 需要阈值量的活化剂来切换诱导型启动子“开”并且组成型启动子“关闭”。 可调节阈值开关对于在阈值浓度或阈值浓度以上的活化剂可逆地切换感兴趣的基因在稳定的“开”状态和稳定的“关闭”状态之间的表达是有用的。
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公开(公告)号：US20130060543A1
公开(公告)日：2013-03-07
申请号：US13655737
申请日：2012-10-19
IPC分类号： G06F17/10
摘要： The present invention provides methods and accompanying computer-based systems and computer-executable code stored on a computer-readable medium for constructing a model of a biological network. The invention further provides methods for performing sensitivity analysis on a biological network and for identifying major regulators of species in the network and of the network as a whole. In addition, the invention provides methods for identifying targets of a perturbation such as that resulting from exposure to a compound or an environmental change. The invention further provides methods for identifying phenotypic mediators that contribute to differences in phenotypes of biological systems.
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公开(公告)号：US06737269B2
公开(公告)日：2004-05-18
申请号：US09872338
申请日：2001-06-01
申请人： Timothy Gardner , James J. Collins
发明人： Timothy Gardner , James J. Collins
IPC分类号： C12N1509
CPC分类号： C12N15/635 , C12N15/63
摘要： Provided are methods and compositions for regulating gene expression in a cell. The invention provides recombinant multi-state genetic oscillators containing an adjustable-threshold genetic switch that is periodically activated by an oscillating amount of an activator agent. The activating agent is a gene product expressed from a promoter regulated by the adjustable-threshold switch and thus forms a feedback loop that causes periodic switching of the adjustable-threshold switch between an “on” state and an “off” state. Multi-state genetic oscillators are useful to express one or more genes of interest in a periodic manner without requiring a periodic application of an external activating agent.
摘要翻译： 提供用于调节细胞中基因表达的方法和组合物。 本发明提供了包含可调节阈值遗传开关的重组多态遗传振荡器，其由振荡量的活化剂周期性地激活。 活化剂是由可调节阈值开关调节的启动子表达的基因产物，因此形成反馈环路，其导致可调节阈值开关在“接通”状态和“关闭”状态之间的周期性切换。 多状态遗传振荡器可用于周期性地表达一种或多种感兴趣的基因而不需要周期性施用外部活化剂。
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公开(公告)号：US20050111097A1
公开(公告)日：2005-05-26
申请号：US10722663
申请日：2003-11-26
申请人： Thomas Iannarelli , Timothy Gardner
发明人： Thomas Iannarelli , Timothy Gardner
CPC分类号： G02B7/002 , G02B23/18 , G02B25/004
摘要： An apparatus for head mounting gear for hands free operation includes a headband and adjustable mounting structure wherein gear such as binoculars and range finders may be selectively mounted, and such gear once mounted may be conveniently raised to a storage position or placed in a use position. The structure includes a quick release mechanism which enables quick and easy interchange between various gear. The headband is preferably comprised of an encircling band and a band that goes over the top of the head. The mounting support structure has adjustments for length from the eyes and height. It is further adjustable into multiple use positions and vertical angle line of sight adjustment.
摘要翻译： 用于免提操作的头部安装齿轮的装置包括头带和可调整的安装结构，其中可以选择性地安装诸如双筒望远镜和测距仪的齿轮，并且一旦安装的这种齿轮可以方便地升高到存储位置或放置在使用位置。 该结构包括快速释放机构，其能够在各种齿轮之间快速和容易地交换。 头带优选地包括环绕带和越过头顶部的带。 安装支撑结构具有从眼睛和高度的长度的调整。 它可以进一步调整到多个使用位置和垂直角度视线调整。
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公开(公告)号：US20100269492A1
公开(公告)日：2010-10-28
申请号：US12430194
申请日：2009-04-27
CPC分类号： F01N3/0231 , F01N3/025 , F01N3/035 , F01N3/0807 , F01N3/0842 , F01N3/106 , F01N3/2066 , F01N13/009 , F01N2240/14 , F01N2610/02 , F01N2610/03 , F01N2610/04 , Y02T10/24
摘要： A diesel exhaust gas aftertreatment system (10) is provided to treat the exhaust (12) from a diesel combustion process (14), such as a diesel compression engine (16). The system (10) includes a burner (18) that selectively supplies the exhaust (12) at an elevated temperature to the rest of the system (10), a diesel particulate filter (20) connected downstream from the burner (18) to receive the exhaust (12) therefrom, and a NOx reducing device (22) connected downstream from the filter (20) to receive the exhaust therefrom.
摘要翻译： 提供柴油机废气后处理系统（10）以从诸如柴油压缩机（16）的柴油机燃烧过程（14）处理废气（12）。 系统（10）包括燃烧器（18），其将升高的温度的排气（12）选择性地供应到系统（10）的其余部分;柴油机微粒过滤器（20），其连接在燃烧器（18）的下游以接收 排气（12），以及连接在过滤器（20）的下游以从其排出的NOx还原装置（22）。
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公开(公告)号：US20120090304A1
公开(公告)日：2012-04-19
申请号：US12907342
申请日：2010-10-19
申请人： Adam J. Kotrba , Jeremy Popovich , Guanyu Zheng , Timothy Gardner , Argun Yetkin
发明人： Adam J. Kotrba , Jeremy Popovich , Guanyu Zheng , Timothy Gardner , Argun Yetkin
IPC分类号： F01N3/10
CPC分类号： F01N3/0211 , F01N3/103 , F01N3/2066 , F01N13/009 , F01N13/011 , F01N2410/00 , F01N2590/02 , F01N2590/08 , F01N2590/10 , Y02A50/2325 , Y02T10/20
摘要： A vehicle exhaust system for an engine having a plurality of combustion chambers includes a first emission treatment device, a second emission treatment device and a housing defining a first exhaust passageway in fluid communication with the combustion chambers and containing the first emission treatment device. A second parallel exhaust passageway is in fluid communication with the combustion chambers and contains the second emission treatment device. The first and second passageways share a common wall having a serpentine shape such that the cross-sectional area of the passageways varies along a direction of exhaust flow. The first emission treatment device is positioned at a location of increased cross-sectional area in the first passageway and the second emission treatment device is positioned at an axially offset location of increased cross-sectional area in the second passageway.
摘要翻译： 一种用于具有多个燃烧室的发动机的排气系统，包括：第一排放处理装置，第二排放处理装置和限定与燃烧室流体连通并且容纳第一排放处理装置的第一排气通道的壳体。 第二平行排气通道与燃烧室流体连通并且包含第二排放处理装置。 第一通道和第二通道共用具有蛇形形状的共同的壁，使得通道的横截面积沿着排气流的方向变化。 第一排放处理装置位于第一通道中的横截面积增加的位置，第二排放处理装置位于第二通道中横截面积增加的轴向偏移位置。
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公开(公告)号：US20120102931A1
公开(公告)日：2012-05-03
申请号：US12915106
申请日：2010-10-29
申请人： Adam J. Kotrba , Jeremy Popovich , Guanyu Zheng , Timothy Gardner , Argun Yetkin
发明人： Adam J. Kotrba , Jeremy Popovich , Guanyu Zheng , Timothy Gardner , Argun Yetkin
CPC分类号： F01N3/2066 , F01N3/025 , F01N3/032 , F01N3/103 , F01N3/2033 , F01N3/2053 , F01N13/0097 , F01N13/017 , F01N2240/36 , F01N2410/04 , Y02T10/24 , Y02T10/26
摘要： An exhaust system for an engine having a plurality of combustion chambers includes a housing containing a first array of parallel positioned emission treatment devices and a second array of parallel positioned emission treatment devices axially spaced apart from one another. A first exhaust passageway is in fluid communication with the combustion chambers and contains the first array of emission treatment devices. A second and separate exhaust passageway is in fluid communication with the combustion chambers and contains the second array of emission treatment devices. A first valve restricts the exhaust flow through the first exhaust passageway. A second valve restricts the exhaust flow through the second exhaust passageway.
摘要翻译： 一种用于具有多个燃烧室的发动机的排气系统，包括：壳体，其容纳并联放置的排放处理装置的第一阵列;以及彼此轴向间隔开的平行放置的排放处理装置的第二阵列。 第一排气通道与燃烧室流体连通并且包含第一阵列的排放处理装置。 第二和分开的排气通道与燃烧室流体连通并且包含第二排放处理装置。 第一阀限制排气流过第一排气通道。 第二阀限制排气流过第二排气通道。
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9.发明申请公开(公告)号：US20070016390A1
公开(公告)日：2007-01-18
申请号：US11174989
申请日：2005-07-05
摘要： The present invention provides methods and accompanying computer-based systems and computer-executable code stored on a computer-readable medium for constructing a model of a biological network. The invention further provides methods for performing sensitivity analysis on a biological network and for identifying major regulators of species in the network and of the network as a whole. In addition, the invention provides methods for identifying targets of a perturbation such as that resulting from exposure to a compound or an environmental change. The invention further provides methods for identifying phenotypic mediators that contribute to differences in phenotypes of biological systems.
摘要翻译： 本发明提供了存储在用于构建生物网络的模型的计算机可读介质上的方法和伴随的基于计算机的系统和计算机可执行代码。 本发明还提供了用于在生物网络上执行灵敏度分析并且用于识别网络和整个网络中物种的主要调节剂的方法。 此外，本发明提供用于鉴定诸如由化合物暴露或环境变化引起的扰动目标的方法。 本发明还提供用于鉴定有助于生物系统表型差异的表型介质的方法。
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10.发明申请公开(公告)号：US20060293873A1
公开(公告)日：2006-12-28
申请号：US10506734
申请日：2003-03-05
申请人： Timothy Gardner , James Collins , Diego di Bernardo , Jesper Tegner , Man Yeung
发明人： Timothy Gardner , James Collins , Diego di Bernardo , Jesper Tegner , Man Yeung
摘要： The present invention provides methods and accompanying computer-based systems and computer-executable code stored on a computer-readable medium for constructing a model of a biological network. The invention further provides methods for performing sensitivity analysis on a biological network and for identifying major regulators of species in the network and of the network as a whole. In addition, the invention provides methods for identifying targets of a perturbation such as that resulting from exposure to a compound or an environmental change. The invention further provides methods for identifying phenotypic mediators that contribute to differences in phenotypes of biological systems.
摘要翻译： 本发明提供了存储在用于构建生物网络的模型的计算机可读介质上的方法和伴随的基于计算机的系统和计算机可执行代码。 本发明还提供了用于在生物网络上执行灵敏度分析并且用于识别网络和整个网络中物种的主要调节剂的方法。 此外，本发明提供用于鉴定诸如由化合物暴露或环境变化引起的扰动目标的方法。 本发明还提供用于鉴定有助于生物系统表型差异的表型介质的方法。
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