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公开(公告)号：US11618876B2
公开(公告)日：2023-04-04
申请号：US15734128
申请日：2019-06-01
申请人： S2 Genomics, Inc.
摘要： A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.
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公开(公告)号：US20230061702A1
公开(公告)日：2023-03-02
申请号：US17891435
申请日：2022-08-19
申请人： Meredian, Inc.
摘要： A method for recovering polyhydroxyalkanoates from a biomass is disclosed. According to the method, polynucleotide chains are cleaved by addition of an endonuclease. A lysing agent is used to disrupt cell walls of the microorganism cells and release the intracellular polyhydroxyalkanoates from the cells. Proteins are also degraded by addition of a peptidase. The polyhydroxyalkanoates are then separated from cellular debris of the cells. According to the present disclosure, this method is carried out without the use of organic solvents in the cleaving, lysing, and degrading steps.
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公开(公告)号：US20230002720A1
公开(公告)日：2023-01-05
申请号：US17903751
申请日：2022-09-06
申请人： VGXI, INC.
发明人： Ruxandra Draghia-Akli , Henry Hebel , Ying Cai
摘要： Large scale processes for producing high purity samples of biologically active molecules of interest from bacterial cells are disclosed. The methods comprise the steps of producing a lysate solution by contacting a cell suspension of said plurality of cells with lysis solution; neutralizing said lysate solution with a neutralizing solution to produce a dispersion that comprises neutralized lysate solution and debris; filtering the dispersion through at least one filter; performing ion exchange separation on said neutralized lysate solution to produce an ion exchange eluate; and performing hydrophobic interaction separation on said ion exchange eluate to produce a hydrophobic interaction solution. Further, provided are compositions comprising large scale amounts of plasmid DNA produced by the disclosed large scale processes.
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公开(公告)号：US20220411743A1
公开(公告)日：2022-12-29
申请号：US17772808
申请日：2020-11-09
申请人： Affibody AB
发明人： Finn Dunås , Belinda Röjstäter , Tomas Moks
摘要： A method of extracting a cytoplasmic or periplasmic protein. The method comprises provision of a first cell suspension comprising cells, the cells containing a cytoplasmic or periplasmic protein of interest to be extracted, the first cell suspension having a first temperature, and heating of the first cell suspension to an operating temperature, at which operating temperature at least a fraction of the cells is subject to heat-induced lysis and at least a fraction of the cytoplasmic or periplasmic protein of interest to be extracted is not subject to irreversible denaturation. The heating of the first cell suspension comprises provision of an aqueous solution, the aqueous solution having a second temperature that is higher than the first temperature, and mixing of the first cell suspension with the aqueous solution, thereby obtaining a second cell suspension, the second cell suspension having a third temperature that is higher than the first temperature. A system for extracting a cytoplasmic or periplasmic protein. The system comprises, i.a., a static mixer.
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公开(公告)号：US20220340864A1
公开(公告)日：2022-10-27
申请号：US17765539
申请日：2020-10-01
发明人： TATSUYA SHIMIZU , YUJI HARAGUCHI , TORU ASAHI , YUTA OKAMOTO
摘要： The present invention provides a production method for a composition for cell culturing. This production method comprises: (1) a step for subjecting algae to an acid hydrolysis treatment and/or an alkali hydrolysis treatment; (2) a step for neutralizing the hydrolysis product obtained in the step (1) to obtain an algae extract; and (3) a step for mixing the algae extract with a medium for cell culturing, wherein the medium for cell culturing does not substantially contain L-glutamine.
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公开(公告)号：US11473049B2
公开(公告)日：2022-10-18
申请号：US16565206
申请日：2019-09-09
发明人： Robert Doebler , Ali Nadim , James D. Sterling , Anna Hickerson , Barbara Erwin , Denice Woyski , Ryan P. Talbot , Bruce Irvine
摘要： Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.
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公开(公告)号：US11453855B2
公开(公告)日：2022-09-27
申请号：US16517985
申请日：2019-07-22
申请人： VGXI, INC.
发明人： Ruxandra Draghia-Akli , Henry Hebel , Ying Cai
摘要： Large scale processes for producing high purity samples of biologically active molecules of interest from bacterial cells are disclosed. The methods comprise the steps of producing a lysate solution by contacting a cell suspension of said plurality of cells with lysis solution; neutralizing said lysate solution with a neutralizing solution to produce a dispersion that comprises neutralized lysate solution and debris; filtering the dispersion through at least one filter; performing ion exchange separation on said neutralized lysate solution to produce an ion exchange eluate; and performing hydrophobic interaction separation on said ion exchange eluate to produce a hydrophobic interaction solution. Further, provided are compositions comprising large scale amounts of plasmid DNA produced by the disclosed large scale processes.
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公开(公告)号：US11446652B2
公开(公告)日：2022-09-20
申请号：US17595802
申请日：2020-05-08
发明人： Jason Bryant , Eric Yeaton , Dan Harris , Josiah Hackendorf , Steven Allen , Mark Talmer
摘要： Magnetic beads having cell components of interest are translated between a sequence of processing wells in a tray without need for pipetting. The circular tray contains one or more sequences of wells each interconnected by a respective channel. The tray is rotated about a central axis and a magnet, an agitator, and a heater provided external to the tray enable magnetic bead translation, mixing, and incubation, respectively. The magnet proximate a well forms a cluster of beads. Manipulation of the tray in rotation and elevation results in translation of the cluster from one well, through a channel, and into an adjacent well. The well containing a cluster may be rotationally positioned in front of the agitator, the agitator extended into contact with the well, followed by mechanical agitation. The heater, disposed beneath the tray, may accept a well lowered thereto for selective heating.
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公开(公告)号：US11434519B2
公开(公告)日：2022-09-06
申请号：US16399305
申请日：2019-04-30
IPC分类号： C12Q1/68 , C12N1/06 , C12Q1/70 , C12Q1/6806 , G01N33/569
摘要： A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.
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公开(公告)号：US20220267713A1
公开(公告)日：2022-08-25
申请号：US17596732
申请日：2020-06-24
发明人： Yunfan LIU , Wenbo WANG , Liming ZHANG , Qian CHEN , Sanmao KANG
摘要： The present invention provides a method for producing a virus and a harvesting solution composition. The method includes culturing cells, wherein the cells have been inoculated with viruses or have been transfected with viral packaging elements; and contacting the cultured cells with a harvesting solution composition to harvest the viruses by one-step, wherein the harvesting solution composition comprises a trypsin, a pH buffer and optionally a nuclease, and the pH of the harvesting solution composition is greater than 7.5 and no more than 10.5. The virus production method of the present invention has advantages of simple operation, easy scale-up, stable yield and so on, and the yield is unexpectedly and significantly improved compared to the prior art, and it can ensure the integrity of the viral particles without damaging the biological activity of the viruses. Therefore, it is very suitable for large-scale production of viruses.
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