公开(公告)号：WO2018180987A1

公开(公告)日：2018-10-04

申请号：PCT/JP2018/011703

申请日：2018-03-23

Applicant: 東洋紡株式会社

Inventor： 岡本　淳 ,  青野　利哉

IPC: C12Q1/6834 ,  C12Q1/6804 ,  G01N33/53 ,  G01N33/543

Abstract: 効率的に核酸を検出するための新たな技術を提供すること。 標的核酸の検出方法であって、 （１）標的核酸にハイブリダイズする標識された捕捉核酸プローブ、及び標的核酸にハイブリダイズする標識された検出核酸プローブを準備する工程 （２）標的核酸を含む試料と捕捉核酸プローブを混合し、標的核酸に捕捉核酸プローブをハイブリダイズさせる工程 （３）標的核酸を含む試料と検出核酸プローブを混合し、標的核酸に検出核酸プローブをハイブリダイズさせる工程 （４）捕捉核酸プローブの標識を特異的に認識する捕捉抗体が固定化された反応器に、捕捉核酸プローブがハイブリダイズした標的核酸を添加し、標的核酸を捕捉抗体に結合させる工程 （５）反応器を洗浄し、標的核酸にハイブリダイズしていない検出核酸プローブを除去する工程、及び （６）検出核酸プローブの標識を検出する工程 を含む、方法。

公开(公告)号：WO2018170003A1

公开(公告)日：2018-09-20

申请号：PCT/US2018/022245

申请日：2018-03-13

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： WOODBURY, Neal ,  ZHAO, Zhan-gong

IPC: B01J19/00 ,  B82Y10/00 ,  C12Q1/00 ,  C12Q1/68 ,  C12Q1/6834

CPC classification number: B01J19/0046 ,  B01J2219/00421 ,  B01J2219/00495 ,  B01J2219/00536 ,  B01J2219/00623 ,  B82Y5/00

Abstract: The manufacturing of molecular arrays often requires the coordination of various physical, chemical, and thermal parameters. Hence, the quality and homogeneity of many molecular arrays can be very dependent on the method of manufacturing. The instant disclosure provides a device that is configured to consistently yield peptide arrays of high quality. The device distributes optimum levels of heat and coupling solution during the chemical coupling and manufacturing of peptide array.

公开(公告)号：WO2018138299A1

公开(公告)日：2018-08-02

申请号：PCT/EP2018/052021

申请日：2018-01-26

Applicant: KATHOLIEKE UNIVERSITEIT LEUVEN

Inventor： CORNELIS, Peter ,  KHORSHID, Mehran ,  WACKERS, Gideon ,  WAGNER, Patrick

IPC: G01N25/48 ,  G01N33/543 ,  C12Q1/6834 ,  C12Q1/6837 ,  G01N33/74 ,  G01N33/94

Abstract: A device suitable for the detection and/or characterization of target particles in a fluid is disclosed. The device comprises: at least one heating element for heating and/or measuring a temperature, the heating element comprising a core comprising at least one electrically conducting portion, an electric isolating layer provided at a surface of the core and electrically isolates the core from the sample, and a plurality of binding sites at/to which target particles can bind. The device further comprising a processing means configured to measure an electric output of the least one heating element, a change of the electric output of the at least one heating element and/or its heating power and for deriving, based thereon, a characteristic of the target particles.

公开(公告)号：WO2018091676A1

公开(公告)日：2018-05-24

申请号：PCT/EP2017/079650

申请日：2017-11-17

Applicant: SPATIAL TRANSCRIPTOMICS AB

Inventor： FRISÉN, Jonas ,  STÅHL, Patrik ,  LUNDEBERG, Joakim ,  SALMÉN, Fredrik ,  VICKOVIC, Sanja

IPC: C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874

Abstract: The present invention relates to methods for spatial tagging of nucleic acid molecules in a biological specimen and in particular to a method comprising: (a) providing a solid substrate on which multiple species of capture probes are immobilized such that each species occupies a distinct position on the solid substrate, wherein said probes are for a primer extension reaction and wherein each species of said capture probes comprise a nucleic acid molecule comprising: (i) a cleavage domain for releasing the capture probe from the surface of the solid substrate, (ii) a positional domain that corresponds to the position of the capture probe on the solid substrate, and (iii) a capture domain; (b) contacting said solid substrate with a biological specimen; and (c) releasing said capture probes from the surface of the solid substrate under conditions that allow nucleic acids of the biological specimen to hybridise to the capture domain in said capture probes and simultaneously and/or subsequently extending said capture probes using the nucleic acid molecules hybridised to the capture probes as extension templates to produce extended probes thereby spatially tagging the nucleic acids of the biological specimen, wherein step (c) comprises contacting said solid substrate with an aqueous reaction mixture comprising: (i) a polymerase enzyme capable of extending said capture probes using the nucleic acid molecules hybridised to the capture probes as extension templates; and (ii) means for releasing said capture probes from the surface of the solid substrate.

公开(公告)号：WO2023069367A1

公开(公告)日：2023-04-27

申请号：PCT/US2022/046906

申请日：2022-10-17

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： LACKEY, Jeremy ,  BANYAI, William

IPC: C12Q1/6869 ,  C12M1/34 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q1/6806

Abstract: Provided herein are compositions, devices, systems and methods for polynucleotide sequencing. Further provided are devices comprising surfaces for continuous sequencing. The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.

公开(公告)号：WO2023023737A1

公开(公告)日：2023-03-02

申请号：PCT/AU2022/050964

申请日：2022-08-23

Applicant: UNIVERSAL BIOSENSORS PTY LTD

Inventor： GREENE IV, George Warren ,  MOULTON, Simon ,  SILVA, Saimon Moraes ,  QUIGLEY, Anita ,  KAPSA, Robert ,  LANGLEY, Daniel Peter

IPC: B01L3/00 ,  G01N27/327 ,  C12Q1/6825 ,  G01N33/574 ,  C12Q1/6834

Abstract: The invention disclosed generally relates to sensors, systems and methods for the detection of an analyte in a complex sample.

公开(公告)号：WO2022271954A1

公开(公告)日：2022-12-29

申请号：PCT/US2022/034734

申请日：2022-06-23

Applicant: ILLUMINA, INC.

Inventor： MANZO, Andrea ,  BROWN, Colin ,  NORBERG, Steven ,  HARRINGTON, Timothy

IPC: C12Q1/6806 ,  C12Q1/6834 ,  G01N33/543 ,  C12N15/10 ,  C12Q1/6816

Abstract: Some embodiments relate to methods and compositions for preparing combinatorially indexed beads. Some embodiments include sequential addition of different indexes to polynucleotides attached to beads. In some embodiments, indexes are added by chemical ligation, polymerase extension, ligation of partially double-stranded adaptors, or short splint ligation.

公开(公告)号：WO2022266714A1

公开(公告)日：2022-12-29

申请号：PCT/AU2022/050637

申请日：2022-06-23

Applicant: ROYAL MELBOURNE INSTITUTE OF TECHNOLOGY ,  PETER MACCALLUM CANCER INSTITUTE

Inventor： AHMED, Taimur ,  PERERA, Ganganath ,  WALIA, Sumeet ,  BHASKARAN, Madhu ,  SRIRAM, Sharath ,  FOX, Stephen ,  FELLOWES, Andrew

IPC: G01N27/12 ,  H01L21/62 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6834

Abstract: The invention provides a sensor for detecting a nucleic acid, comprising: a substrate; a pair of terminal electrodes disposed on the substrate in mutually spaced apart and opposing relation; and a sensing element, between and in electrical contact with the pair of terminal electrodes, wherein the sensing element comprises: (i) a semiconducting portion of the substrate, wherein a conduction path between the terminal electrodes passes through the semiconducting portion; and (ii) an oligonucleotide on a surface of the semiconducting portion, the oligonucleotide being complementary to the nucleic acid to be detected, wherein hybridisation of the nucleic acid with the oligonucleotide leads to a change in resistance of the sensor.

公开(公告)号：WO2022266513A2

公开(公告)日：2022-12-22

申请号：PCT/US2022/034110

申请日：2022-06-17

Applicant: MAMMOTH BIOSCIENCES, INC.

Inventor： PATNO, Timothy James ,  LEE, Phillip You Fai ,  BLIZARD, Benjamin Andrew ,  MIAO, Xin ,  LESINSKI, Jacob ,  BROWN, Ryan A. ,  DRZAL, Daniel Thomas ,  SHAPIRO, Sarah Jane ,  BAIK, Joshua ,  CHEN, Janice Sha ,  BROUGHTON, James Paul ,  FASCHING, Clare Louise ,  CHING, Jesus ,  JAIN, Sonal ,  VERMA, Deepika ,  VEROSLOFF, Matthew ,  SPRATT, Devin ,  FANTIN, Nicholas John

IPC: C12Q1/6844 ,  C12Q1/6834 ,  C12N9/22 ,  C12Q1/6806 ,  C12Q1/686

Abstract: The present disclosure provides various systems, diagnostic devices, and methods for nucleic acid analysis. The systems, devices, and methods allow for the analysis of nucleic acids, in a sample, via programmable nuclease-based assays. Provided are systems comprising an instrument and a cartridge allowing for point of care use. The systems, devices, and methods described herein can be configured for multiplexed detection of nucleic acids in a single sample.

公开(公告)号：WO2022235949A2

公开(公告)日：2022-11-10

申请号：PCT/US2022/027893

申请日：2022-05-05

Applicant: SALUS DISCOVERY, LLC ,  FLAMBEAU DIAGNOSTICS, LLC

Inventor： BEEBE, Dave ,  MCMINN, Patrick ,  MULLINS, Brianna ,  WARRICK, Jay

IPC: C12N15/10 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/558 ,  G01N35/00 ,  B01L1/00 ,  B01L3/00 ,  B03C1/00 ,  B03C1/32 ,  C12Q1/6834 ,  G01N1/40 ,  B01J20/103 ,  B01J20/28042 ,  B01J20/28069 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0829 ,  B01L3/502761 ,  C12Q1/6851 ,  C12Q1/701 ,  G01N33/54326

Abstract: Systems, devices, compositions and methods for positioning and/or processing a target are provided. The invention includes systems, devices, methods and related compositions useful, for example, for the separation, isolation, purification, identification, detection and quantification of materials. Also provided are systems and methods for isolation, and/or detection and/or quantification of a target or analyte in a sample. Some systems, devices, compositions and methods comprise oil and aqueous phases stabilized in close proximity to each other. Some systems, devices and methods use a magnetic force to draw a target or carrier-bound through multiple layers. In some embodiments, systems and devices comprise reagents for detection of a target or analyte.