摘要:
An apparatus and method for making a photometric absorbance measurement of hemoglobin in a blood sample utilizing a reusable cuvette (10) and an inexpensive photometer. The cuvette (10) is a unitary plastic body having a flow-through slot (15) of a predetermined height which allows an absorbance measurement to be made with an undiluted blood sample and which permits cleaning by inserting the body in a liquid cleaning solution. Two absorbance measurements are made, the first at a wavelength at which the absorbance of oxyhemoglobin and deoxyhemoglobin are approximately equal, near an isobestic point, and a second measurement at which these components absorb substantially no light. By making the first measurement near an absorbance minimum at about 510 nm, an absorbance measurement can be made with a relatively thick slot.
摘要:
The method according to the invention is a method of photometric in vitro determination of the content of oxygen in a blood sample. The blood sample is transferred directly from an in vivo locality to an at least partially transparent sample container of a sampling device. The sample container has a measuring chamber wherein a luminophor is provided, whereof the luminescence is quenched in the presence of oxygen. The oxygen content is determined on the basis of the radiation detected by the radiation detector. A sampling device and a system for photometric in vitro determination of oxygen in a blood sample is also described.
摘要:
The method is a method of photometric in vitro determination of a blood gas parameter in a blood sample. The blood sample is transferred directly from an in vivo locality to an at least partially transparent sample container, whereafter the connection between the sample containing sample container and the blood circulation is broken. Subsequently the sample container with its content of blood sample is brought into optical communication with an optical system comprising a radiation source and a radiation detector interacting therewith, preferably by placing the sample container in a sample container system in an analyzer. The blood gas parameter is determined on the basis of the radiation detected by the radiation detector. A system for photometric in vitro determination of a blood gas parameter in a blood sample is also described.
摘要:
This invention measures the change of a fluid's refractive index with changes in the concentration of a solute dissolved therein. A determination of this quantity is required for many types of chemical analyses especially for the determination of molecular weights. The fluid is restricted to a thin capillary channel (11) within a transparent material (10) such as glass. A fine light beam (18) is incident upon the capillary at an angle close to the critical angle. The axes of the light beam and capillary intersect at a point within the capillary defining thereby a plane within which the refraction occurs. A position sensing (27) device is placed to measure the displacement of the beam twice refracted during its passage through the capillary channel, said measure being used to generate a numerical value of the ratio dn/dc, where dc is the change of solute concentration resulting in a change dn of the solution's refractive index.
摘要:
A method for detecting an analyte reactive towards luminol, comprising the steps of: feeding into a reaction chamber an alkaline solution of luminol, noble metal nanoparticles and at least one analyte reactive towards luminol, wherein the reaction chamber is in the form of a curved channel; detecting the light emitted due to a chemiluminescence reaction taking place in said channel; and discharging a reaction mass from said channel, characterized in that the average diameter of the metal nanoparticles is greater than 25nm. Also provided is a microfluidic device for carrying out the method.
摘要:
Described herein are methods and apparatus for spectroscopic analysis of samples. In many embodiments, an apparatus for providing spectroscopic analysis of a sample comprises a sample holder. For example, the sample holder may comprise a consumable single use sample holder that can be readily coupled to and removed from a measurement apparatus such as a spectrometer. The sample holder may comprise a measurement surface configured to receive the sample during measurement, wherein the measurement surface may comprise a porous mesh. The porous mesh can receive the sample to optimally configure the sample for spectroscopic measurement, as described in further detail herein.
摘要:
The invention provides methods and devices for generating optical pulses in one or more waveguides using a spatially scanning light source. A detection system, methods of use thereof and kits for detecting a biologically active analyte molecule are also provided. The system includes a scanning light source, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, a detector that is coupled to and in optical communication with the substrate, and means for spatially translating a light beam emitted from said scanning light source such that the light beam is coupled to and in optical communication with the waveguides of the substrate at some point along its scanning path. The use of a scanning light source al lows the coupling of light into the waveguides of the substrate in a simple and cost-effective manner.
摘要:
An optical waveguide comprises multiple layers of solid-state material disposed on a substrate. One of the layers is a lifting-gate valve made of a high refractive index material. The device provides for better optical confinement in microfluidic channels, and has the capability to integrate both optical signals and fluid sample processing. The optical paths on the chip are reconfigurable because of the use of a movable microvalve that guides light in one of its positions.