公开(公告)号：WO2008097342A3

公开(公告)日：2008-08-14

申请号：PCT/US2007/074751

申请日：2007-07-30

Applicant: SIGMA-ALDRICH CO. ,  CHEN, Fuqiang ,  KREADER, Carol

Inventor： CHEN, Fuqiang ,  KREADER, Carol

IPC: B01D15/00 ,  B01D24/00

Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.

公开(公告)号：WO2007100934A2

公开(公告)日：2007-09-07

申请号：PCT/US2007/060575

申请日：2007-01-16

Applicant: SIGMA-ALDRICH CO. ,  CHEN, Fuqiang ,  KREADER, Carol

Inventor： CHEN, Fuqiang ,  KREADER, Carol

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: The present invention provides extraction compositions and methods for the rapid and efficient isolation of small RNA molecules from a biological sample. In particular, the extraction compositions, when contacted with a biological sample, releases the small RNA molecules from the other molecules in a biological sample, and the released small RNA molecules may then be isolated.

Abstract translation: 本发明提供了用于从生物样品中快速且有效地分离小RNA分子的提取组合物和方法。 特别地，当与生物样品接触时，提取组合物从生物样品中的其他分子释放小RNA分子，然后可以分离释放的小RNA分子。

公开(公告)号：WO2014089290A1

公开(公告)日：2014-06-12

申请号：PCT/US2013/073307

申请日：2013-12-05

Applicant: SIGMA-ALDRICH CO. LLC ,  CHEN, Fuqiang ,  DAVIS, Gregory D. ,  KANG, Qiaohua ,  KNIGHT, Scott W.

Inventor： CHEN, Fuqiang ,  DAVIS, Gregory D. ,  KANG, Qiaohua ,  KNIGHT, Scott W.

IPC: A61K45/00

CPC classification number: C12N15/907 ,  A61K38/00 ,  C07K7/06 ,  C07K14/463 ,  C07K2319/09 ,  C07K2319/10 ,  C07K2319/81 ,  C12N7/00 ,  C12N9/22 ,  C12N9/96 ,  C12N15/102 ,  C12N15/11 ,  C12N15/63 ,  C12N15/67 ,  C12N15/85 ,  C12N15/86 ,  C12N2310/3513 ,  C12N2750/14143 ,  C12N2800/22 ,  C12N2800/80 ,  C12Y301/00 ,  C12Y301/21004

Abstract: The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.

Abstract translation: 本发明提供了用于在真核细胞或胚胎中表达的RNA引导的内切核酸酶，以及使用RNA引导的内切核酸酶在真核细胞或胚胎中靶向基因组修饰的方法。 还提供融合蛋白，其中每个融合蛋白包含CRISPR / Cas样蛋白或其片段和效应结构域。 效应结构域可以是切割结构域，表观遗传修饰结构域，转录激活结构域或转录阻遏物结构域。 还提供了使用融合蛋白修饰染色体序列或调节染色体序列表达的方法。

公开(公告)号：WO2007100934A3

公开(公告)日：2007-12-06

申请号：PCT/US2007060575

申请日：2007-01-16

Applicant: SIGMA ALDRICH CO ,  CHEN FUQIANG ,  KREADER CAROL

Inventor： CHEN FUQIANG ,  KREADER CAROL

IPC: C12N5/06 ,  C12P21/08

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: The present invention provides extraction compositions and methods for the rapid and efficient isolation of small RNA molecules from a biological sample. In particular, the extraction compositions, when contacted with a biological sample, releases the small RNA molecules from the other molecules in a biological sample, and the released small RNA molecules may then be isolated.

Abstract translation: 本发明提供了用于从生物样品快速和有效地分离小RNA分子的提取组合物和方法。 特别地，当与生物样品接触时，提取组合物从生物样品中的其他分子释放小RNA分子，然后可以分离所释放的小RNA分子。

公开(公告)号：WO2005111059A3

公开(公告)日：2006-04-27

申请号：PCT/US2005013376

申请日：2005-04-20

Applicant: SIGMA ALDRICH CO ,  RAY KEVIN B ,  KREADER CAROL A ,  CHEN FUQIANG ,  CUTTER DAVID E

Inventor： RAY KEVIN B ,  KREADER CAROL A ,  CHEN FUQIANG ,  CUTTER DAVID E

IPC: C07H21/04 ,  C12N15/10 ,  C12N15/74 ,  C12Q1/68

CPC classification number: C07H21/04 ,  C12N15/101

Abstract: The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography.

Abstract translation: 本发明提供使用碳水化合物非离子洗涤剂与二氧化硅色谱法还原质粒制备中的内毒素的方法。