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    A METHOD FOR REGULATING CYTOSOLIC ISOPRENOID BIOSYNTHESIS IN HEVEA BRASILIENSIS
    21.
    发明申请
    A METHOD FOR REGULATING CYTOSOLIC ISOPRENOID BIOSYNTHESIS IN HEVEA BRASILIENSIS 审中-公开
    一种用于调节血管性支气管炎生物活性的方法

    公开(公告)号：WO2013039378A1

    公开(公告)日：2013-03-21

    申请号：PCT/MY2012/000214

    申请日：2012-07-23

    Applicant: UNIVERSITI SAINS MALAYSIA ,  MAQSUDUL, Alam ,  MOHD NAZALAN, Mohd Najimudin ,  SAITO, Jennifer Ann ,  BISWAPRIYA, Biswavas Misra

    Inventor： MAQSUDUL, Alam ,  MOHD NAZALAN, Mohd Najimudin ,  SAITO, Jennifer Ann ,  BISWAPRIYA, Biswavas Misra

    IPC: C12N9/10 ,  C12N9/02 ,  C12N9/12 ,  C12N9/14 ,  A01H5/00 ,  C12N15/29 ,  C12N15/52

    CPC classification number: C12N15/8243 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/88 ,  C12Y101/01034 ,  C12Y203/01009 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y401/01033

    Abstract: The present invention discloses an isolated polynucleotide encoding an enzyme for catalyzing cytosolic biosynthesis of isopentenyl diphosphate, dimefhylallyl diphosphate or the combination thereof in the plant of Hevea brasiliensis , comprising nucleotide sequence set forth in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, SEQ ID NO. 7, SEQ' ID NO. 9 or any complementary sequence thereof; and a method for enhancing cytoplasmic availability and functionality of the enzyme, comprising the steps of predicting catalytic domain, signal peptide or membrane-spanning domain of the enzyme; introducing a point mutation to the predicted catalytic domain, signal peptide or membrane-spanning transmembrane domain; and expressing the mutated catalytic domain, signal peptide or membrane-spanning transmembrane domain in a plant cell, tissue or organ of Hevea brasiliensis . The present invention also relates to an isolated polypeptide encoded by the polynucleotide, a recombinant gene construct comprising the polynucleotide, a transformant and a transgenic plant comprising the recombinant gene construct, with enhanced production of cytosolic isoprenoid towards rubber production.

    Abstract translation: 本发明公开了一种分离的多核苷酸，其编码用于催化异二烯基二磷酸，二烯丙基二磷酸或其组合的细胞质生物合成的酶，其包含在SEQ ID NO。 1，SEQ ID NO： 3，SEQ ID NO： 5，SEQ ID NO： 7，SEQ ID NO： 9或其任何互补序列; 以及用于增强酶的细胞质可用性和功能的方法，包括预测酶的催化结构域，信号肽或跨膜结构域的步骤; 对预测的催化结构域，信号肽或跨膜跨膜结构域引入点突变; 并在巴西三叶草的植物细胞，组织或器官中表达突变的催化结构域，信号肽或跨膜跨膜结构域。 本发明还涉及由多核苷酸编码的分离的多肽，包含多核苷酸的重组基因构建体，转化体和包含重组基因构建体的转基因植物，其具有增强的产生细胞质类异戊二烯用于橡胶生产的功能。

    METHODS AND COMPOSITIONS FOR DETECTING MICROBIAL PRODUCTION OF WATER-IMMISCIBLE COMPOUNDS
    23.
    发明申请
    METHODS AND COMPOSITIONS FOR DETECTING MICROBIAL PRODUCTION OF WATER-IMMISCIBLE COMPOUNDS 审中-公开
    检测水中不溶性化合物微生物的方法和组合物

    公开(公告)号：WO2012158466A1

    公开(公告)日：2012-11-22

    申请号：PCT/US2012/037351

    申请日：2012-05-10

    Applicant: AMYRIS, INC. ,  UBERSAX, Jeff ,  FRENZ, Lucas

    Inventor： UBERSAX, Jeff ,  FRENZ, Lucas

    IPC: G01N33/58 ,  C12P5/00

    CPC classification number: C12Q1/02 ,  C12N9/0006 ,  C12P5/007 ,  C12Y101/01034 ,  C12Y203/01009 ,  C12Y203/0301 ,  G01N33/582

    Abstract: Provided herein are methods and compositions useful for detecting the production of compounds in a cell, for example, a microbial cell genetically modified to produce one or more such compounds at greater yield and/or with increased persistence compared to a parent microbial cell that is not genetically modified. In some embodiments, the methods comprise contacting a solution with a fluorescent dye that directly binds the recombinantly produced compound, wherein the solution comprises a plurality of cells recombinantly producing the compound; and detecting the fluorescent dye under spectral conditions suitable for the selective detection of the fluorescent dye bound to the recombinantly produced compound.

    Abstract translation: 本文提供了用于检测细胞中化合物的生产的方法和组合物，例如，与不是亲本微生物细胞相比，经遗传修饰以产生一种或多种此类化合物以更高的产率和/或具有增加的持久性的微生物细胞 转基因 在一些实施方案中，所述方法包括使溶液与直接结合重组产生的化合物的荧光染料接触，其中所述溶液包含重组产生所述化合物的多个细胞; 并在适于选择性检测与重组产生的化合物结合的荧光染料的光谱条件下检测荧光染料。

    TYPE II FATTY ACID SYSTHESIS ENZYMES IN REVERSE B-OXIDATION
    29.
    发明申请
    TYPE II FATTY ACID SYSTHESIS ENZYMES IN REVERSE B-OXIDATION 审中-公开
    II型脂肪酸在反向氧化中的合成酶

    公开(公告)号：WO2015112988A1

    公开(公告)日：2015-07-30

    申请号：PCT/US2015/012932

    申请日：2015-01-26

    Applicant: WILLIAM MARSH RICE UNIVERSITY

    Inventor： GONZALEZ, Ramon ,  CLOMBURG, James, M. ,  VICK, Jacob, E.

    IPC: C10L1/06

    CPC classification number: C12P7/64 ,  C10L1/02 ,  C10L1/04 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/011 ,  C12Y101/03013 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y103/01009 ,  C12Y114/00 ,  C12Y203/01009 ,  C12Y206/00 ,  C12Y401/99005 ,  C12Y402/01059 ,  Y02E50/343

    Abstract: This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis ("FAS") pathway that can be used in combination with thiolases to operate a functional reversal of the β-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the β-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their α-, β-, and ω-functionalized derivatives from renewable carbon sources can be achieved.

    Abstract translation: 本公开描述了可以与硫醇酶组合使用以操作β-氧化循环的功能逆转的来自II型（一组离散的酶）脂肪酸合成（“FAS”）途径的酶。 3-硫基酶与一个或多个3-氧基酰基 - [酰基 - 载体 - 蛋白]还原酶（FabG，其它），3-羟基酰基 - [acp]脱水酶（FabA，FabZ等）和烯酰基 - - 蛋白]还原酶（FabI，FabK，FabL，FabV等）产生β-氧化循环的功能逆转。 如果只使用一种或两种酶，剩余的酶将是传统的β氧化酶。 一旦该循环与适当的引发和终止途径相结合，就可以实现羧酸，醇，烃，胺及其可再生碳源的α-，β-和ω-官能化衍生物的生产。

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