公开(公告)号：WO1995029985A1

公开(公告)日：1995-11-09

申请号：PCT/US1995005391

申请日：1995-04-27

Applicant: RESEARCH DEVELOPMENT FOUNDATION

Inventor： RESEARCH DEVELOPMENT FOUNDATION ,  KOZEL, Thomas, R.

IPC: C12N01/00

CPC classification number: G01N33/569 ,  C12P19/04 ,  G01N33/6854

Abstract: The present invention provides a method of identifying individuals who are at high risk for development of cryptococcosis, comprising the step of measuring the levels of IgG antibody to glucuronoxylomannan in the sera of said individuals. Also provided are various methods of detecting antibody to glucuronoxylomannan in sera and a kit for assaying the presence of antibody to glucuronoxylomannan in the sera of immunocompromised individuals.

Abstract translation: 本发明提供了一种识别处于隐球菌病发展的高风险个体的方法，其包括测量所述个体血清中葡萄糖醛氧基甘露聚糖的IgG抗体水平的步骤。 还提供了各种检测血清中葡糖醛酸氧基甘露聚糖的抗体的方法和用于测定免疫受损个体血清中葡萄糖醛氧乙醇的抗体的存在的试剂盒。

公开(公告)号：WO1993001273A1

公开(公告)日：1993-01-21

申请号：PCT/US1992005806

申请日：1992-07-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  RUSH-PRESBYTERIAN

Inventor： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  RUSH-PRESBYTERIAN ,  LEVY, Jay, A. ,  LANDAY, Alan, L.

IPC: C12N01/00

CPC classification number: C07K14/005 ,  C12N7/00 ,  C12N2710/10322 ,  C12N2710/16122 ,  C12N2710/16222 ,  C12N2710/16522 ,  C12N2710/16722 ,  C12N2740/14022 ,  C12N2740/16022 ,  C12N2760/18422 ,  C12N2770/32322 ,  C12N2770/36222 ,  G01N33/5091 ,  G01N33/56972 ,  Y10S435/975

Abstract: The subject invention permits diagnosis of chronic fatigue syndrome (CFS) by analyzing peripheral blood mononuclear cell subset populations and activation markers. A positive diagnosis for CFS is associated with an increase in the percentage of CD8+ cells showing CD38 or HLA-DR cell markers or a decrease in CD11b markers in CD8+ cells. The analysis is preferably performed using fluorochrome-labelled monoclonal antibodies specific for a determinant of the above subset cells and activation markers.

Abstract translation: 本发明允许通过分析外周血单核细胞亚群和激活标记来诊断慢性疲劳综合征（CFS）。 CFS的阳性诊断与CD38或HLA-DR细胞标志物的CD8 +细胞的百分比增加或CD8 +细胞中CD11b标志物的减少相关。 优选使用对上述子集细胞和活化标记物的决定簇特异的荧光染料标记的单克隆抗体进行分析。

公开(公告)号：WO1992007934A1

公开(公告)日：1992-05-14

申请号：PCT/US1991007887

申请日：1991-10-31

Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

Inventor： IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. ,  KRAMER, Theodore, T.

IPC: C12N01/00

CPC classification number: C12R1/42 ,  A61K39/0275 ,  A61K2039/522 ,  A61K2039/552 ,  C12N1/36 ,  Y02A50/482 ,  Y10S435/879

Abstract: Disclosed herein are methods for attenuating virulent Gram negative bacteria to produce avirulent bacteria. The methods comprise passaging the wild-type bacteria through phagocytic cells, such as macrophages or polymorphonuclear leukocytes, or through lysosomes derived from such cells, a sufficient number of times until the bacteria become avirulent to the animal host. The bacteria are preferably from the family Enterobacteracea and most preferably from the genus Salmonellae. The invention further comprises the avirulent bacteria produced by the methods, pure cultures of such bacteria, and methods of using the bacteria, preferably in a vaccine for administration to an animal host to induce an immune response to the wild-type Gram negative bacteria in the host.

Abstract translation: 本文公开了用于减毒毒性革兰氏阴性细菌以产生无毒力细菌的方法。 所述方法包括通过吞噬细胞（例如巨噬细胞或多形核白细胞）或通过来源于这些细胞的溶酶体传代野生型细菌，直到细菌无毒给动物宿主为止。 细菌优选来自肠杆菌属，最优选来自沙门氏菌属。 本发明还包括通过这些方法产生的无毒力细菌，这种细菌的纯培养物，以及使用细菌的方法，优选在疫苗中给动物宿主诱导对野生型革兰氏阴性菌的免疫应答 主办。

公开(公告)号：WO1990000191A1

公开(公告)日：1990-01-11

申请号：PCT/US1989002809

申请日：1989-06-27

Applicant: THE SALK INSTITUTE BIOTECHNOLOGY/INDUSTRIAL ...

Inventor： THE SALK INSTITUTE BIOTECHNOLOGY/INDUSTRIAL ... ,  TSCHOPP, Juerg, Friedrich ,  DIXON, Douglas, Brian

IPC: C12N01/00

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N15/815 ,  C12N2760/20122

Abstract: The present invention provides a method of producing a rabies glycoprotein G by culturing cells of a methylotrophic species of yeast, which have a gene capable of expressing the pre-form of the rabies glycoprotein G in such cells, under conditions such that the gene is transcribed. The preferred species of yeast for carrying out the invention is Pichia pastoris. Mature rabies glycoprotein G is expressed at a high level in methylotrophic yeast cells as an extractable, membrane-associated glycoprotein. Also provided by the invention are DNAs capable of transforming methylotrophic yeast to express rabies pre-glycoprotein Gs, cultures of cells of such a yeast transformed with such DNAs and the novel glycosylated rabies glycoprotein Gs made by the method of the invention. The rabies glycoprotein G provided by the invention is useful as an immunogen in vaccines to protect mammals against infection by rabies virus.

Abstract translation: 本发明提供了通过培养酵母的甲基营养物质的细胞来生产狂犬病糖蛋白G的方法，所述酵母的细胞在具有能够在该细胞中表达狂犬病糖蛋白G的前体形式的基因的条件下， 。 用于实施本发明的优选酵母菌种是巴斯德毕赤酵母。 成熟的狂犬病糖蛋白G在甲基营养酵母细胞中以高水平表达，作为可提取的膜相关糖蛋白。 本发明还提供能够转化甲基营养酵母以表达狂犬病前糖蛋白G的DNA，用这种DNA转化的酵母的细胞培养物和通过本发明的方法制备的新的糖基化狂犬病糖蛋白Gs。 本发明提供的狂犬病糖蛋白G可用作疫苗中的免疫原，以保护哺乳动物免受狂犬病毒感染。

公开(公告)号：WO1989009815A1

公开(公告)日：1989-10-19

申请号：PCT/US1989001379

申请日：1989-04-04

Applicant: RESEARCH CORPORATION TECHNOLOGIES, INC.

Inventor： RESEARCH CORPORATION TECHNOLOGIES, INC. ,  SHAW, George, M. ,  HAHN, Beatrice, H. ,  KONG, Lilly, I. ,  LEE, Shei-Wen

IPC: C12N01/00

CPC classification number: C07K14/005 ,  A61K39/00 ,  C07K16/1045 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12Q1/703

Abstract: The present invention relates to a novel virus, said virus belonging to the HIV-2 family of viruses. In particular, the invention relates to a retrovirus characterized by being antigenically more similar to, but genetically distinct from, HIV-2/ROD and SIVMAC than to HIV-1. More particularly, the present invention relates to the isolation and cultivation of HIV-2/ST thereby providing a source of said virus and its derivatives including virus-derived antigens and derivatives and parts thereof, useful in the development of diagnostic assays for said virus or other related viruses belonging to the HIV-2 family. Furthermore, the present invention is also directed to recombinant DNA molecules containing the entire HIV-2/ST provirus thereby providing a source of recombinant viral components useful in the development of said diagnostic assays for HIV-2 viruses. The present invention is also directed to antibodies specific to viral components and to different antibodies specific to the first antibodies, said antibodies are also useful in the development of diagnostic assays for HIV-2 viruses. The present invention also contemplates a method of the isolation of HIV-2/ST-type viruses.

Abstract translation: 本发明涉及一种新型病毒，所述病毒属于HIV-2病毒家族。 特别地，本发明涉及一种逆转录病毒，其特征在于与HIV-1相比，其在抗原上与HIV-2 / ROD和SIVMAC更相似，但在遗传上不同。 更具体地，本发明涉及HIV-2 / ST的分离和培养，从而提供所述病毒及其衍生物的来源，所述病毒及其衍生物包括病毒衍生抗原及其衍生物及其部分，可用于开发用于所述病毒的诊断测定或 属于HIV-2家族的其他相关病毒。 此外，本发明还涉及含有整个HIV-2 / ST原病毒的重组DNA分子，从而提供可用于开发用于HIV-2病毒的所述诊断测定的重组病毒组分的来源。 本发明还涉及针对病毒组分和针对第一抗体特异性的不同抗体的抗体，所述抗体也可用于开发用于HIV-2病毒的诊断测定。 本发明还考虑了分离HIV-2 / ST型病毒的方法。

公开(公告)号：WO1987004181A1

公开(公告)日：1987-07-16

申请号：PCT/US1987000044

申请日：1987-01-05

Applicant: CALGENE, INC. ,  STALKER, David, M.

Inventor： CALGENE, INC.

IPC: C12N01/00

CPC classification number: C12N15/63 ,  C12N9/78 ,  C12N15/64 ,  C12N15/70 ,  C12N15/8274

Abstract: Nitrilase enzymes specific for the hydrolysis of the nitrile group of bromoxynil, nucleotide sequences encoding for such enzymes, and transformed cells in which the nitrilase expression is foreign. The transformed cells are capable of expressing the nitrilase enzyme to provide detoxification of an environment and protect bromoxynil-sensitive cells from its cytotoxic effect. Particularly, plants are developed which are resistant to bromoxynil.

Abstract translation: 对溴苯腈的腈基进行水解的腈水解酶，编码这些酶的核苷酸序列以及腈水解酶表达是外来的转化细胞。 转化细胞能够表达腈水解酶以提供环境的解毒，并保护溴苯腈敏感细胞免受其细胞毒性作用。 特别地，开发出抗溴苯腈耐受性的植物。

公开(公告)号：WO1984001959A1

公开(公告)日：1984-05-24

申请号：PCT/US1983001786

申请日：1983-11-16

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  BLANCH, Harvey, W. ,  WILKE, Charles, R. ,  ROY, T., Bruce, Vick

Inventor： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

IPC: C12N01/00

CPC classification number: C12M29/16 ,  C12M29/14 ,  C12P7/56

Abstract: A method and apparatus for continuous cultivation of microbial, animal or plant cells, particularly bacteria for producing lactic acid, employing a reactor comprising at least two microporous walled hollow fibers (13, 14) having an open port at one end and a sealed port (15) at the other end. The mass transfer of nutrients to the cells in the reactor shell space (19) and of products from the cells is enhanced by convective currents between the first (13) and second (14) fibers which results in attainment of improved cell density and productivity of desirable cell products.

Abstract translation: 一种用于连续培养微生物，动物或植物细胞，特别是用于生产乳酸的细菌的方法和装置，其使用包含至少两个在一端具有开放端口的密封空心纤维（13,14）的反应器和密封端口 15）在另一端。 通过在第一（13）和第二（14）纤维之间的对流电流，将营养物质传递到反应器壳体空间（19）中的细胞和来自细胞的产物的质量转移导致改善的细胞密度和生产率 理想的电池产品。

公开(公告)号：WO1998017779A1

公开(公告)日：1998-04-30

申请号：PCT/US1997019196

申请日：1997-10-23

Applicant: SMITHKLINE BEECHAM CORPORATION ,  GENTRY, Daniel, Robert ,  LONSDALE, John, Timothy ,  PAYNE, David, John ,  PEARSON, Stewart, Campbell

Inventor： SMITHKLINE BEECHAM CORPORATION

IPC: C12N01/00

CPC classification number: C12N9/1029 ,  A01K2217/05 ,  A61K38/00

Abstract: The invention provides FabH polypeptides and DNA (RNA) encoding such FabH and a procedure for producing such polypeptiddes by recombinant techniques. Also provided are methods for utilizing such FabH for the treatment of infection, particularly bacterial infections. Antagonists against such FabH and their use as a therapeutic to treat infections, particularly bacterial infections are also provided. Further provided are diagnostic assays for detecting deseases related to the presence of FabH nucleic acid sequences and the polypeptides in a host. Also provided are diagnostic assays for detecting polynucleotides encoding novel Fab family proteins and for detecting such polypeptides in a host.

Abstract translation: 本发明提供FabH多肽和编码这种FabH的DNA（RNA）和通过重组技术产生这种多肽的方法。 还提供了利用这种FabH来治疗感染，特别是细菌感染的方法。 还提供了抗这种FabH的拮抗剂及其用作治疗感染，特别是细菌感染的治疗剂。 进一步提供了用于检测与宿主中FabH核酸序列和多肽的存在相关的病症的诊断测定。 还提供了用于检测编码新型Fab家族蛋白的多核苷酸并用于在宿主中检测此类多肽的诊断测定。

公开(公告)号：WO1997032969A1

公开(公告)日：1997-09-12

申请号：PCT/JP1996001414

申请日：1996-05-23

Applicant: TAKASHIMA, Yasuhide

IPC: C12N01/00

CPC classification number: C12N1/00 ,  C02F3/34

Abstract: Four types of microorganisms, i.e., "aerobic-light" bacteria, "aerobic-dark" bacteria, "anaerobic-light" bacteria, and "anaerobic-dark" bacteria, are allowed to coexist and symbiose in mutual coprosperity in a given space under artificial conditions, which has hitherto been regarded as impossible in the conventional simple, double and parallel double fermentation techniques. This method cuts off all of oxidation, deterioration, and putrefaction, creates only the reduction process of fermentation, decomposition and synthesis and the antioxidation process (oxidation and reduction being simultaneously present), utilizes the function and substrate possessed by the microorganisms and information as informational microbial engineering, and freely uses proliferation, induction, and fermentation to permit all the aerobic and anaerobic organisms to coexist and symbiose in mutual coprosperity.

Abstract translation: 允许四种类型的微生物，即“好氧光”细菌，“需氧 - 黑暗”细菌，“厌氧光”细菌和“厌氧黑暗”细菌在给定的空间内相互共生共生 人造条件迄今被认为在传统的简单，双重和平行双重发酵技术中是不可能的。 这种方法切断了氧化，变质和腐败的所有方法，仅产生发酵，分解和合成的还原过程和抗氧化过程（氧化还原同时存在），利用微生物和信息所具有的功能和底物作为信息 微生物工程，并自由使用增殖，诱导和发酵，以使所有有氧和厌氧生物共存并共生共生。

公开(公告)号：WO1994007992A1

公开(公告)日：1994-04-14

申请号：PCT/US1993009502

申请日：1993-10-05

Applicant: THE GENERAL HOSPITAL CORPORATION ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： THE GENERAL HOSPITAL CORPORATION ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  REED, Guy, L.

IPC: C12N01/00

CPC classification number: G01N33/86 ,  A61K38/00 ,  C07K14/3153 ,  C07K16/40 ,  G01N2333/968 ,  G01N2333/972

Abstract: The present invention is directed to nucleic acids which encode polypeptides that bind with specificity to plasminogen and which correspond to regions of streptokinase. The invention is also directed to vectors and hosts which express such nucleic acids and to the polypeptides themselves. The binding of various purified, cleaved recombinant streptokinase fragments to 125I-plasminogen is shown. In addition, the invention is directed to the use of the claimed polypeptides in assays which detect the presence of plasminogen. Streptokinase fragments which retain their ability to activate plasminogen may be used therapeutically.

Abstract translation: 本发明涉及编码与纤溶酶原特异性结合且对应于链激酶区域的多肽的核酸。 本发明还涉及表达这种核酸和多肽本身的载体和宿主。 显示各种纯化的，切割的重组链激酶片段与125I-纤溶酶原的结合。 此外，本发明涉及所要求保护的多肽在检测纤溶酶原的存在的测定中的用途。 保留其激活纤溶酶原的能力的链激酶片段可以在治疗上使用。