公开(公告)号：WO2005001109A2

公开(公告)日：2005-01-06

申请号：PCT/US2004014374

申请日：2004-05-06

Applicant: NAT INST HEALTH ,  NYAN DOUGBEH C ,  COLEMAN WILLIAM G JR ,  WELCH ANTHONY R

Inventor： NYAN DOUGBEH C ,  COLEMAN WILLIAM G JR ,  WELCH ANTHONY R

IPC: C12Q20060101 ,  C12Q1/68 ,  C12Q

CPC classification number: C12Q1/689

Abstract: The invention provides a method to test for the presence of a microbe in a fecal sample, a method to determine if the population of a gastrointestinal microbe within an organism increases or decreases over time, a method to test the efficacy of a vaccine against a Helicobacter bacterium, kits, and a nucleic acid segment that specifically hybridizes to H. pylori species found in humans.

Abstract translation: 本发明提供了一种测试粪便样品中微生物的存在的方法，一种确定生物体内胃肠微生物群体随时间增加或减少的方法，测试疫苗针对幽门螺杆菌的功效的方法 细菌，试剂盒和与人类中发现的幽门螺杆菌物种特异性杂交的核酸片段。

公开(公告)号：WO2004042072A3

公开(公告)日：2004-12-29

申请号：PCT/US0334386

申请日：2003-10-30

Applicant: UNIV COLORADO A BODY ,  PERRYMAN BENJAMIN M ,  HELMKE STEVE M ,  DUNCAN MARK W

Inventor： PERRYMAN BENJAMIN M ,  HELMKE STEVE M ,  DUNCAN MARK W

IPC: C12Q20060101 ,  G01N24/00 ,  G01N33/00 ,  G01N33/68 ,  H01J49/00

CPC classification number: G01N33/6848 ,  G01N33/6851

Abstract: The present invention provides for methods of quantitating the amounts of proteins or peptides, including those that are closely related isoforms, using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Measurement of protein concentrations in vivo has been extremely difficult and problematic, and protein concentrations have not been shown to correlate well with mRNA levels, the standard used in the past. The present invention overcomes the deficiencies of prior methodologies by taking advantage of MALDI-TOF-MS technology and applying it to proteins and peptides in a way that allows for accurate, quantitative measurement in vivo of protein or peptide concentrations.

公开(公告)号：WO2004081221A3

公开(公告)日：2004-12-23

申请号：PCT/IB2004000692

申请日：2004-03-04

Applicant: INGENEUS CORP ,  ERIKSON GLEN H ,  DAKSIS JASMINE I

Inventor： ERIKSON GLEN H ,  DAKSIS JASMINE I

IPC: C12Q20060101 ,  C12Q1/68

CPC classification number: C12Q1/6832 ,  C12Q2563/173 ,  C12Q2527/137

Abstract: A method for assaying a nucleobase-containing target with a nucleobase-containing probe, wherein: (a) the target is pre-incubated with at least one target incubation agent prior to being mixed with the probe; and/or (b) the probe is pre-incubated with at least one probe incubation agent prior to being mixed with the target to form the hybridization mixture. The pre-incubation enhances the signal to noise ratio of the assay. The pre-incubation medium and/or the hybridization medium can be pretreated with electric voltage. A kit for performing the method includes the probe, a label adapted to emit the signal, and at least one target incubation agent and/or probe incubation agent.

Abstract translation: 一种用含核碱基的探针分析含核碱基的靶的方法，其中：（a）在与探针混合之前用至少一种靶孵育剂预温育靶; 和/或（b）在与靶标混合形成杂交混合物之前，将探针与至少一种探针孵育剂一起预温育。 预温育增强了测定的信噪比。 预孵育培养基和/或杂交培养基可以用电压预处理。 用于执行该方法的试剂盒包括探针，适于发射信号的标签以及至少一种靶标温育剂和/或探针温育剂。

公开(公告)号：WO2004048600A3

公开(公告)日：2004-10-07

申请号：PCT/US0337749

申请日：2003-11-24

Applicant: UNIV EMORY ,  GEWIRTZ ANDREW ,  SITARAMAN SHANTI V ,  KAPPROTH JAN MICHAEL

Inventor： GEWIRTZ ANDREW ,  SITARAMAN SHANTI V ,  KAPPROTH JAN MICHAEL

IPC: C12Q20060101 ,  G01N33/53 ,  G01N33/68

CPC classification number: G01N33/6893 ,  G01N2800/065

Abstract: Methods are disclosed for diagnosing inflammatory bowel disease (IBD), one exemplar method of which includes determining whether a sample is positive for anti-flagellin antibodies (AFA), diagnosing the presence of IBD when the sample is positive for AFA, and diagnosing the absence of IBD when the sample is negative for AFA. Alternatively, instead of testing for positivity with respect to AFA, or in addition thereto, an exemplar method can determine whether the sample has an AFA level above an AFA cut-off value (X); and diagnosing IBD when' the AFA level is above X, and diagnosing absence of IBD when the AFA level is below X. Test kits that include items used to carry out the disclosed methods are also disclosed.

Abstract translation: 公开了用于诊断炎症性肠病（IBD）的方法，其一种示例性方法包括确定样品是否对抗鞭毛蛋白抗体（AFA）呈阳性，当样品对AFA呈阳性时诊断IBD的存在，以及诊断不存在 当样本为AFA阴性时，IBD的样本。 或者，代替检测关于AFA的积极性，或除此之外，示例性方法可确定样品是否具有高于AFA截止值（X）的AFA水平; 并且当AFA水平高于X时诊断IBD，并且当AFA水平低于X时诊断IBD缺乏。还公开了包括用于执行所公开的方法的项目的测试工具。

公开(公告)号：WO2004072296A2

公开(公告)日：2004-08-26

申请号：PCT/US2004002592

申请日：2004-01-30

Applicant: GEN HOSPITAL CORP ,  POZNANSKY MARK C ,  BRAINARD DIANA M

Inventor： POZNANSKY MARK C ,  BRAINARD DIANA M

IPC: A61K38/11 ,  A61K38/16 ,  A61K39/21 ,  C07K14/16 ,  C12Q20060101 ,  C12Q1/70 ,  G01N33/53 ,  C12Q

CPC classification number: C07K14/005 ,  A61K38/00 ,  A61K38/162 ,  C12N2740/16122

Abstract: The invention relates to a new method for measuring cytotoxic activity of immune cells, and to methods and products for treating abnormal immune responses.

Abstract translation: 本发明涉及一种测定免疫细胞的细胞毒活性的新方法，以及用于治疗异常免疫应答的方法和产品。

公开(公告)号：WO2004016802A3

公开(公告)日：2004-08-12

申请号：PCT/US0324721

申请日：2003-08-06

Applicant: PROTEOPLEX INC ,  LABRIE SAM ,  MATHUR AKASH ,  LAWRY KIM ,  PANCIO HEATHER ,  DANIELSON NATHAN

Inventor： LABRIE SAM ,  MATHUR AKASH ,  LAWRY KIM ,  PANCIO HEATHER ,  DANIELSON NATHAN

IPC: C12N1/00 ,  C12Q20060101 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/551

CPC classification number: G01N33/5306

Abstract: The present invention discloses methods and apparatus for the detection of biological molecules in various samples. In particular, a method for detecting a hapten-labeled analyte in a solution wherein substantially all other solution constituents are labeled with a hapten is disclosed. The method may be used to detect single or multiple analytes in solution (Figure 1). Apparatus to detect such analytes are also disclosed.

Abstract translation: 本发明公开了用于检测各种样品中生物分子的方法和装置。 特别地，公开了一种用于检测溶液中半抗原标记分析物的方法，其中基本上所有其它溶液组分用半抗原标记。 该方法可用于检测溶液中的单一或多个分析物（图1）。 还公开了检测这种分析物的装置。

公开(公告)号：WO2004015133A3

公开(公告)日：2004-08-12

申请号：PCT/US0308761

申请日：2003-03-20

Applicant: CALIFORNIA INST OF TECHN ,  VAN DAM MICHAEL ,  UNGER MARC A ,  QUAKE STEPHEN R

Inventor： VAN DAM MICHAEL ,  UNGER MARC A ,  QUAKE STEPHEN R

IPC: B01J19/00 ,  B81B1/00 ,  C07B61/00 ,  C12Q20060101 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  C40B60/14 ,  G01N1/00 ,  G01N15/06 ,  G01N21/00 ,  G01N21/29 ,  G01N27/00 ,  G01N27/12 ,  G01N33/00 ,  G01N33/48

CPC classification number: G01N15/06 ,  B01J19/0046 ,  B01J2219/00355 ,  B01J2219/00398 ,  B01J2219/00418 ,  B01J2219/00427 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B82Y30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  C40B60/14 ,  Y10T436/10 ,  Y10T436/25 ,  Y10T436/255 ,  Y10T436/2575

Abstract: The present invention provides a microfluidic device for synthesizing an array of compounds and methods for using the same. In particular, the microfluidic device of the present invention comprises a solid support base, an elastomeric layer attached to the solid support, and a plurality of flow channels located within the interface between the solid support and the elastomeric layer. In addition, the solid support comprises a functional group for forming a bond with a reactive reagent. In some embodiments, the microfluidic device further comprises a second plurality of flow channels that intersect the first plurality of flow channels. A plurality of control channels are also present in the microfluidic devices of the present invention. The control channels can be actuated to regulate flow of fluids within the flow channel(s).

Abstract translation: 本发明提供了用于合成化合物阵列的微流体装置及其使用方法。 特别地，本发明的微流体装置包括固体支撑基底，连接到固体支撑体的弹性体层，以及位于固体支撑体和弹性体层之间的界面内的多个流动通道。 此外，固体支持物包含与反应试剂形成键的官能团。 在一些实施例中，微流体装置还包括与第一多个流动通道相交的第二多个流动通道。 多个控制通道也存在于本发明的微流体装置中。 可以启动控制通道以调节流动通道内的流体的流动。

公开(公告)号：WO2004005534A3

公开(公告)日：2004-04-29

申请号：PCT/US0322259

申请日：2003-07-08

Applicant: UNIV DUKE ,  PERICAK-VANCE MARGARET A ,  VANCE JEFFREY M ,  HAINES JONATHAN L

Inventor： PERICAK-VANCE MARGARET A ,  VANCE JEFFREY M ,  HAINES JONATHAN L

IPC: C07H21/04 ,  C12P19/34 ,  C12Q20060101 ,  C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/172

Abstract: The present invention discloses methods of screening a subject for Alzheimer's disease comprising detecting the presence or absence of a marker or functional polymorphism associated with a gene linked to Alzheimer's disease.

Abstract translation: 本发明公开了筛选受试者阿尔茨海默氏病的方法，包括检测与阿尔茨海默病相关基因相关的标志物或功能多态性的存在或不存在。

公开(公告)号：WO2004027078A2

公开(公告)日：2004-04-01

申请号：PCT/US0329248

申请日：2003-09-17

Applicant: UNIV PENNSYLVANIA

Inventor： PERCEC VIRGIL

IPC: B82B1/00 ,  B32B5/16 ,  C07C49/788 ,  C07C69/92 ,  C07C205/55 ,  C07C205/57 ,  C07D209/86 ,  C09K19/32 ,  C09K19/34 ,  C12Q20060101 ,  F21V9/00 ,  C12Q

CPC classification number: C09K19/32 ,  B82Y30/00 ,  C07C49/788 ,  C07C69/92 ,  C07C205/57 ,  C07C2603/18 ,  C07C2603/50 ,  C07D209/86 ,  C09K19/322 ,  C09K19/3477 ,  C09K2019/0407 ,  C09K2019/0418 ,  C09K2019/0429 ,  C09K2019/044 ,  C09K2019/328 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995

Abstract: This invention relates to novel fluorinated dendrons and to a universal strategy for producing functional fluorinated dendrons programmed to self-assemble into supramolecular nanocylinder compositions containing pstacks of high electron or hole mobility donors (D), acceptors (A), or D-A complexes in the core. Such nanocylinder compositions are uniquely applicable to devices spanning from single supramolecule to nanoscopic and to macroscopic scales including transistors, photovoltaics, photoconductors, photorefractives, light emissives, and optoelectronics.

Abstract translation: 本发明涉及新型氟化树枝状大分子以及制备官能化氟化树枝状大分子的通用策略，所述氟化树枝化程序被编程为自组装成含有高电子或空穴迁移率供体（D），受体（A）或核心中的DA复合物的叠层的超分子纳米圆柱体组合物 。 这种纳米圆柱组合物特别适用于从单一超分子到纳米级以及宏观尺度的器件，包括晶体管，光伏器件，光电导体，光折变器，光发射器和光电子器件。

公开(公告)号：WO02072863A8

公开(公告)日：2004-02-19

申请号：PCT/US0206685

申请日：2002-03-05

Applicant: APPLERA CORP ,  HALPERN BENJAMIN R

Inventor： HALPERN BENJAMIN R

IPC: G01N27/62 ,  C12P21/06 ,  C12Q20060101 ,  G01N33/48 ,  G06K9/00 ,  H01J49/04 ,  H01J49/40

CPC classification number: H01J49/0036 ,  G06F19/22 ,  H01J49/0431

Abstract: The present invention provides methods for matching a sample of an unknown query peptide to a database of known peptides. The methods described herein allow for the rapid, sensitive, and selective identification of an unknown query peptide, which enables the development of high throughput protein identification. The methods described herein also allow for mass spectrometry data for a query peptide to be categorized and weighted according to its quality. Furthermore, the methods described herein provide robust identification of modified query proteins by either anticipating modifications or adjusting for modified peptide masses.

Abstract translation: 本发明提供了将未知查询肽的样品与已知肽的数据库进行匹配的方法。 本文描述的方法允许对未知查询肽的快速，灵敏和选择性鉴定，其能够开发高通量蛋白质鉴定。 本文描述的方法还允许将查询肽的质谱数据根据其质量进行分类和加权。 此外，本文所述的方法通过预期修饰或调整修饰的肽质量来提供修饰的查询蛋白的强力鉴定。