公开(公告)号：WO01064024A1

公开(公告)日：2001-09-07

申请号：PCT/US2001/006276

申请日：2001-02-28

IPC分类号： C07K14/325 ,  C12N15/79 ,  C12N15/82 ,  A01H5/02 ,  A01H5/10 ,  A01H13/00 ,  C12N1/20 ,  C12N1/12 ,  C12N5/04 ,  C12N15/29 ,  C12N15/32 ,  C12N15/52 ,  C12N15/31 ,  C12N15/17 ,  C12N15/13 ,  C12N15/63 ,  C12N15/74

CPC分类号： C07K14/325 ,  C12N15/79 ,  C12N15/8214 ,  C12N15/8241 ,  C12N15/8259 ,  C12N15/8271 ,  C12N15/8286 ,  Y02A40/162

摘要： Introducing blocks of foreign genes in a single operon would avoid complications such as position effect and gene silencing inherent in putting one gene at a time into random locations in the nuclear genome. Cloning several genes into a single T-DNA does not avoid the compounded variable expression problem encountered in nuclear transgenic plants. This disclosure shows that a bacterial operon can be expressed in a single integration event as opposed to multiple events requiring several years to accomplish. Expression of multiple genes via a single transformation event opens the possibility of expressing foreign pathways or pharmaceutical proteins involving multiple genes. Expressing the Cry2aA2 operon, including a putative chaperonin to aid in protein folding, in the chloroplast via a single transformation event leads to production of crystalized insecticidal proteins. Expressing the Mer operon via a single transformation event leads to a phytoremediation system.

摘要翻译： 将外源基因块导入单个操纵子避免了基因定位和失活等并发症，这些并发症一次将基因置于随机位置。 的核基因组。 将几个基因克隆到单个T-DNA中并不能避免在转基因核植物中出现的化合物可变表达问题。 本发明证明细菌操纵子可以在单个整合事件中表达，而不是需要几年发生的多个事件。 通过单个转化事件表达多个基因提供了表达外来途径或涉及多个基因的药物蛋白质的可能性。 通过单一转化事件表达Cry2aA2操纵子，包括促进蛋白质折叠的通常已知的伴侣蛋白在叶绿体中的表达导致产生结晶的杀虫蛋白质。 通过单一转化事件表达Mer操纵子提供植物修复系统。

公开(公告)号：WO01002579A1

公开(公告)日：2001-01-11

申请号：PCT/FR2000/001856

申请日：2000-06-30

IPC分类号： C07K14/32 ,  C12N15/31 ,  C12N15/32 ,  C12N15/82 ,  C07K14/325 ,  A01H5/00 ,  A01N63/00

CPC分类号： C07K14/32 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention concerns a synthetic coding for a Bacillus thuringiensis CryIC toxin and transgenic plants expressing said gene.

摘要翻译： 本发明涉及对苏云金芽孢杆菌CryIC毒素和表达所述基因的转基因植物的合成编码。

公开(公告)号：WO0066742A2

公开(公告)日：2000-11-09

申请号：PCT/US0012136

申请日：2000-05-03

申请人： MONSANTO CO

发明人： RUPAR MARK J ,  DONOVAN WILLIAM P ,  CHU CHIH-REI ,  PEASE ELIZABETH ,  TAN YUPING ,  SLANEY ANNETTE C ,  MALVAR THOMAS M ,  BAUM JAMES A

IPC分类号： A01N63/02 ,  C07K14/325 ,  C12N1/21 ,  C12N15/32 ,  C12N15/82 ,  A01H5/00 ,  A01N63/00

CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

摘要： Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed delta -endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

摘要翻译： 公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。 还公开了载体，转化的宿主细胞和含有编码所公开的δ-内毒素多肽的核酸片段的转基因植物。 还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予 对植物对目标昆虫物种的抗性。

公开(公告)号：WO0011200A3

公开(公告)日：2000-08-31

申请号：PCT/US9919102

申请日：1999-08-18

申请人： MONSANTO CO

发明人： CONNER TIMOTHY W ,  SANTINO COLLEEN G

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/32 ,  C12N15/82 ,  A01H5/00 ,  C12N5/10

CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N15/8282 ,  C12N15/8286 ,  Y02A40/162

摘要： Novel combinations of 5', 3' and intron genetic elements are provided for enhanced expression in transgenic plants. The elements are associated with a fructose 1,6-bisphosphatase gene, a chlorophyll a/b binding protein gene, a ubiquitin gene, a nopaline synthase gene, and/or a heat shock gene. Recombinant DNA molecules containing the non-translated 5' and/or 3' non-translated elements of the invention are further provided, as are plant cells, tissues and plants containing those DNA molecules.

摘要翻译： 提供了5'，3'和内含子遗传元件的新型组合，用于在转基因植物中增强表达。 这些元件与果糖1,6-二磷酸酶基因，叶绿素a / b结合蛋白基因，泛素基因，胭脂碱合酶基因和/或热休克基因相关。 还提供了含有本发明的非翻译5'和/或3'非翻译元件的重组DNA分子，以及含有那些DNA分子的植物细胞，组织和植物。

公开(公告)号：WO00024905A1

公开(公告)日：2000-05-04

申请号：PCT/US1999/024648

申请日：1999-10-21

IPC分类号： C07K14/325 ,  C12N15/32 ,  C12N15/82 ,  C12N5/10 ,  A01H5/00

CPC分类号： C07K14/325 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention provides new, plant-optimized polynucleotide sequences that encode pesticidal toxins. The polynucleotide sequences of the subject invention have certain modifications, compared to wild-type sequences, that make them particularly well-suited for optimized expression in plants. Using the polynucleotide sequences described herein, the transformation of plants can be accomplished, using techniques known to those skilled in the art, in order to confer pest resistance upon said plants. In preferred embodiments, the subject invention provides plant-opimized polynucleotide sequences which encode toxin proteins that can be referred to as cytC-type toxins. A preferred toxin encoded by a plant-optimized gene is also disclosed.

摘要翻译： 本发明涉及可用于防治害虫，特别是植物害虫的材料和方法。 更具体地，本发明提供新的植物优化的编码杀虫毒素的多核苷酸序列。 与野生型序列相比，本发明的多核苷酸序列具有某些修饰，使得它们特别适用于在植物中优化表达。 使用本文所述的多核苷酸序列，可以使用本领域技术人员已知的技术来实现植物的转化，以便赋予所述植物上的害虫抗性。 在优选的实施方案中，本发明提供编码可称为cytC型毒素的毒素蛋白质的植物修饰的多核苷酸序列。 还公开了由植物优化的基因编码的优选毒素。

公开(公告)号：WO00011025A1

公开(公告)日：2000-03-02

申请号：PCT/FI1999/000698

申请日：1999-08-24

IPC分类号： A01N63/00 ,  C07K14/325 ,  C12N15/32 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8216 ,  A01N63/00 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The present invention is related to modified, synthetic DNA sequences allowing improved insect control due to their improved mRNA procession, stability and their enhanced translation in higher plants. Said synthetic DNA sequences comprise useful tools for resisitance management, especially for overcoming cross-resistance problems. The improvements are achieved by providing synthetic modifications of the truncated DNA sequence (SEQ ID NO:3:) of the cry9Aa gene, preferably SEQ ID NO:2: coding for a protein characterized by having an amino acid sequence SEQ ID NO:1: or alterations thereof still having substantially the same insecticidal action as the insecticidal protein encoded by the cry9Aa gene of Bacillus thuringiensis ssp. galleria (Btg). Also disclosed is the use of the modified, synthetic DNA sequences for producing said insecticidal protein and for producing transgenic plants expressing effective amounts of the insecticidal proteins.

摘要翻译： 本发明涉及修饰的合成DNA序列，其允许改进的昆虫控制，这是由于它们改进的mRNA加成，稳定性及其在高等植物中的增强翻译。 所述合成DNA序列包括用于电阻管理的有用工具，特别是用于克服交叉阻力问题。 通过提供cry9Aa基因的截短DNA序列（SEQ ID NO：3 :)的合成修饰来实现改进，优选SEQ ID NO：2：编码特征在于具有氨基酸序列SEQ ID NO：1的蛋白质： 或其改变仍然具有与由苏云金芽孢杆菌（Bacillus thuringiensis）ssp的cry9Aa基因编码的杀虫蛋白基本相同的杀虫作用。 galleria（Btg）。 还公开了修饰的合成DNA序列用于产生所述杀虫蛋白和用于产生表达有效量的杀虫蛋白的转基因植物的用途。

公开(公告)号：WO99057282A2

公开(公告)日：1999-11-11

申请号：PCT/US1999/009997

申请日：1999-05-06

IPC分类号： A01H5/00 ,  A01N63/00 ,  C07K14/325 ,  C12N5/10 ,  C12N15/09 ,  C12N15/32 ,  C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/8286 ,  A01N63/00 ,  C07K14/325 ,  C12Q1/689 ,  C12Q2600/142 ,  C12Q2600/156 ,  Y02A40/162

摘要： Disclosed and claimed are novel Bacillus thuringiensis isolates, pesticidal toxins, genes, and nucleotide probes and primers for the identification of genes encoding toxins active against pests. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The subject invention provides entirely new families of toxins which can be obtained from the supernatants of Bacillus cultures.

摘要翻译： 公开和要求的是新型苏云金芽孢杆菌分离株，杀虫毒素，基因和核苷酸探针和引物，用于鉴定编码针对害虫的毒素的基因。 引物在PCR技术中可用于产生编码这些毒素的基因特征的基因片段。 本发明提供了可以从芽孢杆菌培养物的上清液中获得的全新的毒素家族。

公开(公告)号：WO9931248A9

公开(公告)日：1999-09-16

申请号：PCT/US9826852

申请日：1998-12-17

申请人： ECOGEN INC ,  MONSANTO CO ,  ENGLISH LEIGH ,  BRUSSOCK SUSAN M ,  MALVAR THOMAS M ,  BRYSON JAMES W ,  KULESZA CAROLINE A ,  WALTERS FREDERICK S ,  SLATIN STEPHEN L ,  VON TERSCH MICHAEL A ,  ROMANO CHARLES

发明人： ENGLISH LEIGH ,  BRUSSOCK SUSAN M ,  MALVAR THOMAS M ,  BRYSON JAMES W ,  KULESZA CAROLINE A ,  WALTERS FREDERICK S ,  SLATIN STEPHEN L ,  VON TERSCH MICHAEL A ,  ROMANO CHARLES

IPC分类号： C07K14/325 ,  C12N15/32 ,  A01H5/00

CPC分类号： C07K14/325

摘要： Disclosed are methods for increasing the activity of B. thuringiensis delta -endotoxins against Coleopteran insect pests. Also disclosed are methods for mutagenizing nucleic acid sequences encoding these polypeptides, and increasing insect resistance in transgenic plants expressing these genes.

摘要翻译： 公开了用于增加苏云金芽孢杆菌δ-内毒素对鞘翅目害虫的活性的方法。 还公开了用于诱变编码这些多肽的核酸序列并增加表达这些基因的转基因植物中的昆虫抗性的方法。