公开(公告)号：WO2011149762A3

公开(公告)日：2012-04-12

申请号：PCT/US2011037179

申请日：2011-05-19

Applicant: SLOAN KETTERING INST CANCER ,  STUDER LORENZ ,  CHAMBERS STUART M ,  GRUBER MICA YVONNE

Inventor： STUDER LORENZ ,  CHAMBERS STUART M ,  GRUBER MICA YVONNE

IPC: G01N33/68 ,  C12N5/0735 ,  C12Q1/68 ,  G01N33/15

CPC classification number: C12N5/0618 ,  C12N5/062 ,  C12N5/0626 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5023 ,  G01N33/5058

Abstract: The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC), human induced pluripotent stem cells (hiPSC), somatic stem cells, cancer stem cells, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and/or hiPSC to nociceptors (i.e. nociceptor cells) using novel culture conditions. The nociceptors made using the methods of the present invention are further contemplated for various uses including, but limited to, use in in vitro drug discovery assays, pain research, and as a therapeutic to reverse disease of, or damage to, the peripheral nervous system (PNS). Further, compositions and methods are provided for producing melanocytes from human pluripotent stem cells for use in disease modeling.

Abstract translation: 本发明涉及干细胞生物学领域，特别是多能干细胞或多能干细胞的细胞特异性分化，其可包括但不限于人胚胎干细胞（hESC），人诱导多能干细胞（hiPSC ），体细胞干细胞，癌症干细胞或能够谱系特异性分化的任何其他细胞。 具体描述的是使用新的培养条件将hESC和/或hiPSC的谱系特异性分化指向伤害感受器（即伤害感受器细胞）的方法。 使用本发明的方法制备的伤害感受器进一步考虑用于各种用途，包括但不限于用于体外药物发现测定，疼痛研究以及作为逆转外周神经系统疾病或损害的治疗剂 （PNS）。 此外，提供用于从人多能干细胞产生用于疾病建模的黑素细胞的组合物和方法。

公开(公告)号：WO2011079017A3

公开(公告)日：2011-10-20

申请号：PCT/US2010060756

申请日：2010-12-16

Applicant: CENTOCOR ORTHO BIOTECH INC ,  XU JEAN

Inventor： XU JEAN

IPC: C12N5/071 ,  C12N5/0735 ,  C12Q1/68

CPC classification number: C12N5/0676 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/70 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2533/90

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells expressing markers characteristic of the pancreatic endocrine lineage that co-express NKX6.1 and insulin and minimal amounts of glucagon.

Abstract translation: 本发明提供促进多能干细胞分化成胰岛素生成细胞的方法。 特别地，本发明提供了一种产生表达特征性胰岛内分泌谱系特异性标志物的方法，其共表达NKX6.1和胰岛素和最小量的胰高血糖素。

公开(公告)号：WO2011017337A3

公开(公告)日：2011-06-09

申请号：PCT/US2010044268

申请日：2010-08-03

Applicant: WISCONSIN ALUMNI RES FOUND ,  ZHANG SU-CHUN ,  ZHANG XIAOQING

Inventor： ZHANG SU-CHUN ,  ZHANG XIAOQING

IPC: C12N5/079

CPC classification number: C12N5/0623 ,  C12N5/0618 ,  C12N5/0696 ,  C12N15/86 ,  C12N2501/40 ,  C12N2501/60 ,  C12N2502/99 ,  C12N2506/02 ,  C12N2506/08 ,  C12N2510/00

Abstract: A transcription factor both necessary and sufficient for human neuroectoderm specification, Pax6, as well as applications thereof, is disclosed.

Abstract translation: 公开了对人类神经外胚层规范Pax6所必需的足够的转录因子及其应用。

公开(公告)号：WO2011063039A1

公开(公告)日：2011-05-26

申请号：PCT/US2010/057102

申请日：2010-11-17

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY ,  BLAU, Helen M. ,  PAJCINI, Kostandin ,  POMERANTZ, Jason

Inventor： BLAU, Helen M. ,  PAJCINI, Kostandin ,  POMERANTZ, Jason

IPC: C12N5/00

CPC classification number: C12N5/0658 ,  A61K31/713 ,  A61K35/34 ,  C12N2501/00 ,  C12N2501/40 ,  C12N2501/405 ,  C12N2506/1323 ,  G01N33/502

Abstract: Methods are provided for producing cells within a lineage (lineage restricted cells) from post-mitotic differentiated cells of the same lineage ex vivo and in vivo, and for treating a subject in need of tissue regeneration therapy by employing these lineage-restricted cells. In addition, the production of lineage restricted cells from postmitotic tissues derived from patients with diseases allows for a characterization of pathways that have gone awry in these diseases and for screening of drugs that will ameliorate or correct the defects as a means of novel drug discovery. Also provided are kits for performing these methods.

Abstract translation: 提供了用于从离体和体内相同谱系的有丝分裂后分化细胞中产生谱系内的细胞（谱系受限细胞）以及用于通过以下方式治疗需要组织再生治疗的受试者的方法： 采用这些谱系限制细胞。 此外，从患有疾病的患者衍生的有丝分裂后组织中产生谱系受限细胞允许表征这些疾病中出现错误的途径以及筛选能够改善或纠正缺陷的药物作为新型药物发现的手段。 还提供了执行这些方法的工具包。

公开(公告)号：WO2011034073A1

公开(公告)日：2011-03-24

申请号：PCT/JP2010/065903

申请日：2010-09-15

Applicant: 国立大学法人東京大学 ,  江藤 浩之 ,  高山 直也 ,  中村 壮 ,  中内 啓光

Inventor： 江藤　浩之 ,  高山　直也 ,  中村　壮 ,  中内　啓光

IPC: C12N5/0789 ,  A61K35/14 ,  A61P7/00 ,  C12N5/10 ,  C12N15/09

CPC classification number: C12N5/0641 ,  A61K35/00 ,  C12N5/0644 ,  C12N2501/40 ,  C12N2501/48 ,  C12N2501/60 ,  C12N2501/606 ,  C12N2506/02 ,  C12N2506/45

Abstract: 　本発明は、所望の分化段階の細胞を増幅し、特定の細胞を製造する方法の提供を目的とする。　本発明は、細胞を分化誘導して特定の細胞を製造する方法であって、所望の分化段階の細胞を増幅するために、当該所望の分化段階の細胞内で癌遺伝子を強制発現させて、特定の細胞の製造する方法を提供する。さらに、当該所望の分化段階の細胞内で発現される癌遺伝子が誘導する癌遺伝子誘導性細胞老化（ＯＩＳ）を抑制し、特定の細胞を製造する方法も提供する。

Abstract translation: 公开了通过在期望的分化阶段扩增细胞来产生特异性细胞的方法。 所公开的方法是诱导细胞分化以产生特定细胞。 为了在期望的分化阶段扩增细胞，致癌基因在所需分化阶段的细胞内强制表达，产生特异性细胞。 此外，所公开的方法抑制致癌基因诱导的衰老（OIS），其由在所需分化阶段的细胞内表达的致癌基因诱导。

公开(公告)号：WO2010111422A2

公开(公告)日：2010-09-30

申请号：PCT/US2010/028541

申请日：2010-03-24

Applicant: THE SALK INSTITUTE FOR BIOLOGICAL STUDIES ,  KAWAMURA, Teruhisa ,  SUZUKI, Jotaro ,  WANG, Yunyuan, V. ,  RAYA, Angel ,  WAHL, Geoffrey, M. ,  IZPISUA BELMONTE, Juan Carlos

Inventor： KAWAMURA, Teruhisa ,  SUZUKI, Jotaro ,  WANG, Yunyuan, V. ,  RAYA, Angel ,  WAHL, Geoffrey, M. ,  IZPISUA BELMONTE, Juan Carlos

IPC: C12N5/074 ,  C12N15/12 ,  C12N5/10

CPC classification number: C12N5/0696 ,  C12N2501/40 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/998 ,  C12N2510/00

Abstract: Methods and compositions are provided for, inter alia , the generation of induced pluripotent stem cells. Induced pluripotent stem cells may be generated by reprogramming and inhibition of p53. Further, useful intermediates for the generation of induced pluripotent stem cells are also provided.

Abstract translation: 提供方法和组合物，尤其是诱导多能干细胞的产生。 诱导的多能干细胞可通过重编程和抑制p53产生。 此外，还提供了用于产生诱导多能干细胞的有用中间体。

公开(公告)号：WO2007053207A3

公开(公告)日：2009-06-18

申请号：PCT/US2006025800

申请日：2006-06-30

Applicant: GEN HOSPITAL CORP ,  LEE JEANNIE T

Inventor： LEE JEANNIE T

IPC: C12N15/09 ,  C12N5/00 ,  C12N5/02 ,  C12N5/0735 ,  C12N5/18 ,  C12N15/11

CPC classification number: C12N15/85 ,  C12N5/0606 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2330/10 ,  C12N2501/40 ,  C12N2501/60 ,  C12N2510/00 ,  C12N2800/30

Abstract: Disclosed herein are methods for controlling stem cell differentiation through the introduction of transgenes having Xic, Tsix, or Xite sequences to block differentiation and the removal of the transgenes to allow differentiation. Also disclosed are small RNA molecules and methods for using the small RNA molecules to control stem cell differentiation. Also disclosed are stem cells genetically modified by the introduction of Xic, Tsix, or Xite sequences.

Abstract translation: 本文公开了通过引入具有Xic，Tsix或Xite序列的转基因来控制干细胞分化的方法，以阻断转基因的分化和去除以允许分化。 还公开了小RNA分子和使用小RNA分子来控制干细胞分化的方法。 还公开了通过引入Xic，Tsix或Xite序列进行遗传修饰的干细胞。

公开(公告)号：WO2009030217A3

公开(公告)日：2009-04-30

申请号：PCT/DE2008001470

申请日：2008-09-03

Applicant: MEDICYTE GMBH ,  BRASPENNING ADRIANUS J C M ,  HOLDER STEFAN ,  KUEPPER HEINER

Inventor： BRASPENNING ADRIANUS J C M ,  HOLDER STEFAN ,  KUEPPER HEINER

IPC: C12N5/06

CPC classification number: C12N5/00 ,  C12N2501/40

Abstract: The invention relates to a method for propagating or concentrating primary cells without tumorous characteristics and to the subsequent use thereof.

Abstract translation: 本发明涉及一种用于传播或原代细胞的富集无肿瘤性能和它们的进一步使用的方法。

公开(公告)号：WO2008091680A3

公开(公告)日：2008-12-18

申请号：PCT/US2008000959

申请日：2008-01-24

Applicant: GEN HOSPITAL CORP ,  LEE JEANNIE T

Inventor： LEE JEANNIE T

IPC: C12N5/00 ,  C12N5/02

CPC classification number: C12N5/0606 ,  C12N5/16 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/85 ,  C12N15/86 ,  C12N15/907 ,  C12N2330/10 ,  C12N2501/065 ,  C12N2501/40 ,  C12N2501/60 ,  C12N2510/00

Abstract: Disclosed herein are methods for controlling stem cell differentiation through the introduction of transgenes having Xic, Tsix, Xite, or Xic flanking region sequences to block differentiation and the removal of the transgenes to allow differentiation. Also disclosed are small RNA molecules and methods for using the small RNA molecules to control stem cell differentiation. Also disclosed are stem cells genetically modified by the introduction of Xic, Tsix, XUe, or Xic flanking region sequences.

Abstract translation: 本文公开了通过引入具有Xic，Tsix，Xite或Xic侧翼区序列的转基因来控制干细胞分化以阻断分化和去除转基因以允许分化的方法。 还公开了小RNA分子和使用小RNA分子来控制干细胞分化的方法。 还公开了通过引入Xic，Tsix，XUe或Xic侧翼区序列进行遗传修饰的干细胞。

公开(公告)号：WO2008106771A1

公开(公告)日：2008-09-12

申请号：PCT/CA2008/000397

申请日：2008-03-03

Applicant: UNGRIN, Mark ,  ZANDSTRA, Peter

Inventor： UNGRIN, Mark ,  ZANDSTRA, Peter

IPC: C12N5/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/32 ,  C12M3/00 ,  C12N5/00 ,  C12N5/08 ,  C12Q1/24

CPC classification number: C12M23/12 ,  B01L3/5085 ,  B01L2200/0668 ,  B01L2300/0893 ,  B01L2400/0409 ,  C12M23/16 ,  C12N5/0603 ,  C12N5/0606 ,  C12N2501/155 ,  C12N2501/40 ,  C12N2501/48 ,  C12N2501/70 ,  C12N2502/13 ,  C12N2533/92

Abstract: The present application provides methods and devices for the production and recovery of cell aggregates. In one embodiment, the device is a microwell device with a high density of microwells. The application also provides a device for extracting cell aggregates such as stem cells or embryoid bodies from well plates. Such cell aggregates are used for the differentiation of pluripotent stem cells such as embryonic stem cells, in the fields of developmental biology and regenerative medicine / tissue engineering.

Abstract translation: 本申请提供了用于生产和回收细胞聚集体的方法和装置。 在一个实施例中，该装置是具有高密度微孔的微孔器件。 本申请还提供了从孔板中提取诸如干细胞或胚状体的细胞聚集体的装置。 这种细胞聚集体用于在发育生物学和再生医学/组织工程领域中多能干细胞如胚胎干细胞的分化。