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    ASSAY FOR DETECTING MYCOBACTERIAL INFECTION
    81.
    发明申请
    ASSAY FOR DETECTING MYCOBACTERIAL INFECTION 审中-公开
    检测MYCOBACTERIAL感染的测定

    公开(公告)号：WO2008135771A1

    公开(公告)日：2008-11-13

    申请号：PCT/GB2008/001596

    申请日：2008-05-08

    Applicant: THE UNIVERSITY OF BIRMINGHAM ,  LALVANI, Ajit ,  MONTAMAT-SICOTTE, Damien, JC ,  WILLCOX, Benjamin, E. ,  BESRA, Gurdyal, S.

    Inventor： LALVANI, Ajit ,  MONTAMAT-SICOTTE, Damien, JC ,  WILLCOX, Benjamin, E. ,  BESRA, Gurdyal, S.

    IPC: G01N33/569 ,  G01N33/50

    CPC classification number: G01N33/5695 ,  G01N33/505 ,  G01N2333/57

    Abstract: A method of assessing a mycobacterial infection in a subject comprising; i. exposing at least one CDl molecule or analogue to mycolic acid or a mycolic acid analogue; ii. subsequently incubating the at least one CDl molecule or analogue with a sample comprising at least one T cell isolated from the subject; iii. measuring the T cell response and/or the number of mycolic acid specific T cells present in the T cell sample. The method can distinguish between active and latent tuberculosis infection and can be used to monitor the effectiveness of therapeutic intervention.

    Abstract translation: 评估受试者中分枝杆菌感染的方法，包括： 一世。 将至少一种CD1分子或类似物暴露于霉酚酸或霉酚酸类似物; II。 随后将所述至少一种CD1分子或类似物与包含从受试者分离的至少一种T细胞的样品孵育; III。 测量T细胞样品中存在的T细胞应答和/或霉菌酸特异性T细胞的数量。 该方法可以区分活动性和潜伏性结核感染，可用于监测治疗干预的有效性。

    CROSS-SPOT
    83.
    发明申请
    CROSS-SPOT 审中-公开

    公开(公告)号：WO2008048097A1

    公开(公告)日：2008-04-24

    申请号：PCT/NL2007/050498

    申请日：2007-10-16

    Applicant: THIJSEN, Steven, Frederik, Theodoor ,  BOSSINK, Ailko, Wim, Jan

    Inventor： BOSSINK, Ailko, Wim, Jan

    IPC: G01N33/50 ,  G01N33/569

    CPC classification number: G01N33/6854 ,  G01N33/505 ,  G01N33/5052 ,  G01N2333/57

    Abstract: The invention relates to an in vitro method for measuring the presence of an antigen indicative for the presence of an infectious agent, and preferably a medical condition or disease in a sample using an immune effector cell capable of producing an immune effector molecule following stimulation by an antigen.

    Abstract translation: 本发明涉及一种体外测定方法，该方法用于使用能够产生免疫效应分子的免疫效应细胞，在刺激后测定存在感染因子的抗原的存在，优选在样品中存在医学病症或疾病 抗原。

    NANOPARTICLE AND MICROPARTICLE BASED DETECTION OF CELLULAR PRODUCTS
    84.
    发明申请
    NANOPARTICLE AND MICROPARTICLE BASED DETECTION OF CELLULAR PRODUCTS 审中-公开
    纳米颗粒和微粒子检测细胞产物

    公开(公告)号：WO2005012505A3

    公开(公告)日：2005-06-16

    申请号：PCT/US2004023582

    申请日：2004-07-23

    Applicant: CELLULAR TECHNOLOGY LTD ,  LEHMANN PAUL V ,  KARULIN ALEXEY Y ,  KLEEN THOMAS OLIVER

    Inventor： LEHMANN PAUL V ,  KARULIN ALEXEY Y ,  KLEEN THOMAS OLIVER

    IPC: G01N33/50 ,  G01N33/533 ,  G01N33/58 ,  G01N3/58

    CPC classification number: G01N33/505 ,  B82Y15/00 ,  G01N33/587 ,  G01N33/588 ,  G01N2333/5409 ,  G01N2333/57 ,  Y10T436/25375

    Abstract: Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon or comprising hydrophobic membranes are described having greater capability to detect products from individual cells in a mixture of heterogeneous cells. With the use of multiple detection particles, multiple cellular products can be detected in a single well. Additionally, using the inherent spectral properties of detection particles, no enzymatic reactions are needed to visualize a secreted product, thereby increasing the sensitivity, reproducibility and ease of use.

    Abstract translation: 本发明的实施方式涉及使用具有产品特异性检测试剂并具有特征性光谱特征的检测颗粒来检测细胞产物的装置和方法。 具体而言，提供了用于测量包括细胞因子的分泌细胞产物的装置和方法。 检测底物包括在其上具有产物特异性捕获试剂或包含疏水性膜的微孔，具有更强的检测异质细胞混合物中各个细胞产物的能力。 通过使用多种检测颗粒，可以在单个孔中检测多种细胞产物。 此外，利用检测颗粒的固有光谱特性，不需要酶促反应来显现分泌的产物，从而增加灵敏度，重现性和易用性。

    NANOPARTICLE AND MICROPARTICLE BASED DETECTION OF CELLULAR PRODUCTS
    85.
    发明申请
    NANOPARTICLE AND MICROPARTICLE BASED DETECTION OF CELLULAR PRODUCTS 审中-公开
    基于纳米颗粒和微孔的蜂窝产品检测

    公开(公告)号：WO2005012505A2

    公开(公告)日：2005-02-10

    申请号：PCT/US2004/023582

    申请日：2004-07-23

    Applicant: CELLULAR TECHNOLOGY LTD. ,  LEHMANN, Paul, V. ,  KARULIN, Alexey, Y. ,  KLEEN, Thomas, Oliver

    Inventor： LEHMANN, Paul, V. ,  KARULIN, Alexey, Y. ,  KLEEN, Thomas, Oliver

    IPC: C12N5/00

    CPC classification number: G01N33/505 ,  B82Y15/00 ,  G01N33/587 ,  G01N33/588 ,  G01N2333/5409 ,  G01N2333/57 ,  Y10T436/25375

    Abstract: Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon or comprising hydrophobic membranes are described having greater capability to detect products from individual cells in a mixture of heterogeneous cells. With the use of multiple detection particles, multiple cellular products can be detected in a single well. Additionally, using the inherent spectral properties of detection particles, no enzymatic reactions are needed to visualize a secreted product, thereby increasing the sensitivity, reproducibility and ease of use.

    Abstract translation: 本发明的实施方案涉及使用具有产品特异性检测试剂并具有特征光谱特征的检测粒子检测细胞产物的装置和方法。 特别地，提供了用于测量包括细胞因子在内的分泌的细胞产物的装置和方法。 描述了检测底物，包括其上具有产物特异性捕获试剂或包含疏水膜的微孔，其具有检测来自异种细胞混合物中的各个细胞的产物的更大能力。 通过使用多个检测粒子，可以在单个孔中检测多个细胞产物。 此外，使用检测颗粒的固有光谱性质，不需要酶反应来可视化分泌产物，从而增加灵敏度，再现性和易用性。

    A NOVEL ASSAY FOR DETECTING IMMUNE RESPONSES INVOLVING ANTIGEN SPECIFIC CYTOKINE AND/OR ANTIGEN SPECIFIC CYTOKINE SECRETING T-CELLS
    88.
    发明申请
    A NOVEL ASSAY FOR DETECTING IMMUNE RESPONSES INVOLVING ANTIGEN SPECIFIC CYTOKINE AND/OR ANTIGEN SPECIFIC CYTOKINE SECRETING T-CELLS 审中-公开
    用于检测涉及抗原特异性细胞因子和/或抗原特异性细胞因子分泌T细胞的免疫应答的新方法

    公开(公告)号：WO02033421A1

    公开(公告)日：2002-04-25

    申请号：PCT/US2001/032442

    申请日：2001-10-18

    IPC: G01N33/50 ,  G01N33/567 ,  G01N33/68

    CPC classification number: G01N33/6866 ,  G01N33/5047 ,  G01N33/567 ,  G01N33/6863 ,  G01N2333/4715 ,  G01N2333/57

    Abstract: Here, we describe a sensitive and specific assay and kit for the detection of chemokines having activity that is upregulated by Th-1 cytolines (such IFN- gamma ) and chemokines that upregulate the activity of Th-1 cytolines (such as IFN- gamma . In a typical embodiment, detection of the chemokine monokine induced by gamma interferon (MIG) provides a measure of the biological effect of IFN- gamma rather than direct quantitation of IFN- gamma or IFN- gamma secreting cell per se. Upregulation of MIG expression was observed following in vitro activation of PBMC with defined CD8 T cell epitopes derived from influenza virus, CMV, or EBV, and in all cases this was antigen-specific, genetically restricted and dependent on both CD8 T cells and IFN- gamma . Responses as assessed by the MIG assay paralleled those detected by conventional IFN- gamma ELISPOT, but the magnitude of response and sensitivity of the MIG assay were superior. Our data validate this novel method for the detection of high as well as low levels of antigen-specific and genetically restricted IFN- gamma activity of MIG.

    Abstract translation: 在这里，我们描述一种灵敏和特异性的测定和试剂盒，用于检测具有Th-1细胞系（如IFN-γ）上调的活性的趋化因子和调节Th-1细胞系活性（如IFN-γ）的趋化因子。 在典型的实施方案中，由γ干扰素（MIG）诱导的趋化因子单核因子的检测提供IFN-γ的生物学效应的量度，而不是直接定量IFN-γ或IFN-γ分泌细胞本身.MIG表达的上调是 在体外激活具有来自流感病毒，CMV或EBV的确定的CD8 + T细胞表位的PBMC后观察到，并且在所有情况下，这是抗原特异性的，遗传限制性的并且依赖于CD8 + T细胞和IFN - 通过MIG测定评估的反应与常规IFN-γELISPOT检测的反应相似，但是MIG测定的响应和灵敏度的大小是优异的。我们的数据验证了这种新的检测方法 高和低水平的抗原特异性和遗传受限的IFN-γ活性的MIG。

    DIAGNOSTIC AND THERAPEUTIC METHODS BASED UPON V alpha 24J alpha Q T CELLS
    89.
    发明申请
    DIAGNOSTIC AND THERAPEUTIC METHODS BASED UPON V alpha 24J alpha Q T CELLS 审中-公开
    基于Vα24JαQT细胞的诊断和治疗方法

    公开(公告)号：WO99034209A1

    公开(公告)日：1999-07-08

    申请号：PCT/US1998/026868

    申请日：1998-12-17

    IPC: G01N33/50 ,  G01N33/564 ,  G01N33/68

    CPC classification number: G01N33/5091 ,  G01N2333/5406 ,  G01N2333/57

    Abstract: The present invention is directed to methods that can be used for diagnosing whether an individual either has, or is likely to develop, an autoimmune disease. The methods are based upon determining the level of CD4 CD8 V alpha 24J alpha Q T cells present in the individual being tested or the pattern of cytokine secretion evidenced by these cells. In addition, the invention is directed to a therapeutic method for treating or preventing autoimmune disease which is based upon the specific expansion of the CD4 CD8 V alpha 24J alpha Q T cell population.

    Abstract translation: 本发明涉及可用于诊断个体是否具有或可能发展自身免疫性疾病的方法。 所述方法基于确定存在于所测试个体中的CD4 + CD8 +Vα24JαQ+ T细胞的水平或由这些细胞证实的细胞因子分泌的模式。 此外，本发明涉及一种治疗或预​​防基于CD4 + CD8 - / - α2αJJC+ T细胞群的特异性扩增的自身免疫性疾病的治疗方法。

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