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公开(公告)号：WO2011059826A3
公开(公告)日：2011-09-29
申请号：PCT/US2010054760
申请日：2010-10-29
Applicant: CALIFORNIA INST OF TECHN , TRUONG THAI V , CHOI JOHN M , FRASER SCOTT E , SUPATTO WILLY , KOOS DAVID S
Inventor： TRUONG THAI V , CHOI JOHN M , FRASER SCOTT E , SUPATTO WILLY , KOOS DAVID S
CPC classification number: G02B21/16 , G01N21/6408 , G01N21/6428 , G01N21/6458 , G01N21/6486 , G01N2021/655 , G02B21/002 , G02B21/367
Abstract: An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.
Abstract translation: 提供了一种多光子激光荧光与光谱显微镜正交照明相结合的多光子光谱显微镜（MP-LISH）的设备和方法。 通过对整个果蝇和斑马鱼胚胎的实时成像，MP-LISH的高性能与目前最先进的成像技术相比，保持生物组织内部的良好信号和高空间分辨率（比单光子光更深两倍） 片状显微镜），在采集速度（比常规双光子激光扫描显微镜快超过一个数量级），并且具有低的光毒性。 这种新的成像技术的固有多模态也被证明了二次谐波发生光谱显微镜检测小鼠尾部组织中的胶原蛋白。 共同的，这些属性为MP-LISH在活体生物系统4D成像中的应用提供了广泛的应用。
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公开(公告)号：WO2011015832A1
公开(公告)日：2011-02-10
申请号：PCT/GB2010/001495
申请日：2010-08-06
Applicant: UNIVERSITY OF STRATHCLYDE , MCCONNELL, Gail
Inventor： MCCONNELL, Gail
CPC classification number: G02B21/16 , G02B21/002 , G02B21/33 , G02B21/36
Abstract: A far field scanning microscope, for example a MPLSM system, comprising a laser source for generating an excitation beam; self-focusing means for causing self-focusing of the excitation beam, thereby to reduce its lateral size; means for scanning the reduced size excitation beam relative to a sample area and means for forming an image of the sample using light reflected from or transmitted through the sample.
Abstract translation: 远场扫描显微镜，例如MPLSM系统，包括用于产生激发光束的激光源; 自聚焦装置，用于引起激发光束的自聚焦，从而减小其横向尺寸; 用于相对于采样区域扫描减小尺寸的激发光束的装置和用于使用从样品反射或透射通过样品的光形成样品的图像的装置。
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公开(公告)号：WO2010108042A3
公开(公告)日：2011-01-13
申请号：PCT/US2010027872
申请日：2010-03-18
Applicant: UNIV UTAH RES FOUND , JACKSON LAB , UNIV MAINE SYS BOARD TRUSTEES , BENNETT BRIAN THOMAS , BEWERSDORF JOERG , JORGENSEN ERIK , HESS SAM , GOULD TRAVIS , GUNEWARDENE MUDALIGE SIYATH
Inventor： BENNETT BRIAN THOMAS , BEWERSDORF JOERG , JORGENSEN ERIK , HESS SAM , GOULD TRAVIS , GUNEWARDENE MUDALIGE SIYATH
IPC: G01N21/64 , G01N33/483 , G02B21/00 , H01J37/26
CPC classification number: H01J37/26 , G01N21/6458 , G02B21/002 , G02B21/16 , G02B21/361 , G02B27/1006 , H01J37/228 , H01J37/28
Abstract: An optical microscope (101) with heightened resolution and capable of providing three dimensional images is disclosed and described. The microscope (101) can include a sample stage (160) for mounting a sample having a plurality of probe molecules. At least one non-coherent light source (127) can be provided. At least one lens (140a, 140b) can be configured to direct a beam of light from the at least one non-coherent light source (127) toward the sample causing the probe molecules to luminesce. A camera (155) can be configured to detect luminescence from the probe molecules. A light beam path modification module (132, 150) can be configured to alter a path length of the probe molecule luminescence to allow camera luminescence detection at a plurality of object planes.
Abstract translation: 公开并描述了具有高分辨率并且能够提供三维图像的光学显微镜（101）。 显微镜（101）可以包括用于安装具有多个探针分子的样品的样品台（160）。 可以提供至少一个非相干光源（127）。 至少一个透镜（140a，140b）可以被配置为将来自至少一个非相干光源（127）的光束引向样品，使得探针分子发光。 相机（155）可被配置为检测来自探针分子的发光。 光束路径修改模块（132,150）可以被配置为改变探针分子发光的路径长度以允许在多个物体平面处的照相机发光检测。
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公开(公告)号：WO2011001635A1
公开(公告)日：2011-01-06
申请号：PCT/JP2010/004183
申请日：2010-06-23
Applicant: 株式会社日立ハイテクノロジーズ , 山口敦子 , 桃井義典 , 田中潤一 , 川田洋揮
IPC: G01B15/04 , G01N23/225 , H01L21/66
CPC classification number: H01J37/28 , G01B2210/56 , G02B21/002 , G02B21/361 , G02B21/365 , G02B21/367 , G06T5/50 , G06T7/0004 , G06T7/0006 , G06T7/0008 , G06T7/001 , G06T2207/10016 , G06T2207/10056 , G06T2207/10061 , G06T2207/20081 , G06T2207/30141 , G06T2207/30148 , H01J37/244 , H01J2237/24578 , H01J2237/24592 , H01J2237/2816 , H01J2237/2817 , H01L22/12 , H01L2924/0002 , H01L2924/00
Abstract: 電子顕微鏡で得られる一視野の中の試料像内において、測定パターン下層の材料・構造に起因したエッジ位置計測誤差の変動を抑制することができる半導体検査装置および半導体検査装置の検査方法を提供するために、第１の方法として、電子線を走査して得られる視野内の観察対象の構造や材料の情報に基づいて、視野内を複数の領域に分割し、領域毎に電子線の走査条件を変える(８０５，８０６)、第２の方法として、取得した画像に対して分割された領域毎に画像処理条件を変える、第３の方法として、取得した画像のエッジ検査領域において分割された領域毎にエッジ検出条件を変える。
Abstract translation: 提供一种半导体检查装置和半导体检查方法，使得在通过电子显微镜获得的单个视场中的样本图像中，可以抑制归因于下部的材料和结构的边缘位置测量误差的变化 通过第一方法测量图案层，其中通过电子束扫描获得的视野中的区域基于关于待观察物体的结构和材料的信息以及电子束扫描被分成多个区域 对于各个区域（805,806）的条件改变，第二种方法，其中，针对由所获得的图像的划分产生的各个区域改变图像处理条件，或者第三种方法，其中针对各个区域改变边缘检测条件 由获得的图像的边缘检查区域内的划分产生。
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公开(公告)号：WO2010120231A1
公开(公告)日：2010-10-21
申请号：PCT/SE2010/050385
申请日：2010-04-09
Applicant: GENERAL ELECTRIC COMPANY , GE HEALTHCARE BIO-SCIENCES AB , KENNY, Kevin, B. , YAZDANFAR, Siavash , HENDERSON, David, L.
Inventor： KENNY, Kevin, B. , YAZDANFAR, Siavash , HENDERSON, David, L.
CPC classification number: G02B21/244 , G02B21/002 , G02B21/245 , G02B21/365
Abstract: In an imaging device having an objective and a stage for holding a sample to be imaged, a method for autofocusing is presented. The method includes determining a measured focus value corresponding to at least a first of a plurality of logical image segments. Further, the method includes imaging the first logical image segment using the measured focus value. The method also includes determining a predicted focus value for a second of the plurality of logical image segments using the measured focus value and a stored focus variation parameter. In addition, the method includes imaging the second logical image segment using the predicted focus value.
Abstract translation: 在具有用于保持要成像的样品的目标和阶段的成像装置中，提出了一种用于自动聚焦的方法。 该方法包括确定对应于多个逻辑图像片段中的至少第一个的测量焦点值。 此外，该方法包括使用测量的聚焦值对第一逻辑图像片段进行成像。 该方法还包括使用测量的聚焦值和存储的聚焦变化参数来确定多个逻辑图像片段中的第二个的预测聚焦值。 此外，该方法包括使用预测的聚焦值来成像第二逻辑图像片段。
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Patent Agency Ranking
公开(公告)号：WO2010108042A2
公开(公告)日：2010-09-23
申请号：PCT/US2010/027872
申请日：2010-03-18
Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION , THE JACKSON LABORATORY , UNIVERSITY OF MAINE SYSTEM BOARD OF TRUSTEES , BENNETT, Brian, Thomas , BEWERSDORF, Joerg , JORGENSEN, Erik , HESS, Sam , GOULD, Travis , GUNEWARDENE, Mudalige, Siyath
Inventor： BENNETT, Brian, Thomas , BEWERSDORF, Joerg , JORGENSEN, Erik , HESS, Sam , GOULD, Travis , GUNEWARDENE, Mudalige, Siyath
IPC: G01N21/64 , G02B21/00 , H01J37/26 , G01N33/483
CPC classification number: H01J37/26 , G01N21/6458 , G02B21/002 , G02B21/16 , G02B21/361 , G02B27/1006 , H01J37/228 , H01J37/28
Abstract: An optical microscope (101) with heightened resolution and capable of providing three dimensional images is disclosed and described. The microscope (101) can include a sample stage (160) for mounting a sample having a plurality of probe molecules. At least one non-coherent light source (127) can be provided. At least one lens (140a, 140b) can be configured to direct a beam of light from the at least one non-coherent light source (127) toward the sample causing the probe molecules to luminesce. A camera (155) can be configured to detect luminescence from the probe molecules. A light beam path modification module (132, 150) can be configured to alter a path length of the probe molecule luminescence to allow camera luminescence detection at a plurality of object planes.
Abstract translation: 公开并描述了具有高分辨率并且能够提供三维图像的光学显微镜（101）。 显微镜（101）可以包括用于安装具有多个探针分子的样品的样品台（160）。 可以提供至少一个非相干光源（127）。 至少一个透镜（140a，140b）可以被配置为将来自至少一个非相干光源（127）的光束引向样品，使得探针分子发光。 相机（155）可被配置为检测来自探针分子的发光。 光束路径修改模块（132,150）可以被配置为改变探针分子发光的路径长度以允许在多个物体平面处的照相机发光检测。
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公开(公告)号：WO2010096786A2
公开(公告)日：2010-08-26
申请号：PCT/US2010024959
申请日：2010-02-22
Applicant: VISIONGATE INC , WATSON MATHEW , HAYENGA JON
Inventor： WATSON MATHEW , HAYENGA JON
IPC: G01B11/24 , B81B7/02 , G01N21/00 , G01N33/483
CPC classification number: G02B21/025 , G01N21/4795 , G02B21/002
Abstract: An object of interest is illuminated within the field of view of a microscope objective lens (10) located to receive light passing through the object of interest. Light transmitted through the microscope objective lens (10) impinges upon a variable power element (33). The variable power element (33) is driven with respect to the microscope objective lens (10) to scan through multiple focal planes in the object of interest. Light transmitted from the variable power element (33) is sensed by a sensing element or array (30).
Abstract translation: 感兴趣的物体在位于接收通过感兴趣物体的光的显微镜物镜（10）的视野内被照亮。 透过显微镜物镜（10）的光照射在可变功率元件（33）上。 可变功率元件（33）相对于显微镜物镜（10）被驱动以扫描感兴趣对象中的多个焦平面。 从可变功率元件（33）发射的光由感测元件或阵列（30）感测。
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公开(公告)号：WO2010095263A1
公开(公告)日：2010-08-26
申请号：PCT/JP2009/053203
申请日：2009-02-23
Inventor： 瀧本 真一
IPC: G02B21/00
CPC classification number: G02B21/16 , G01J3/44 , G01N21/65 , G01N2021/653 , G02B21/002 , G02B21/0088
Abstract: レーザ照射光学系３，４，５，６により、ＣＡＲＳ用レーザ光とラマン散乱用レーザ光とを同軸で標本に照射して、ＣＡＲＳ光をＣＡＲＳ光検出手段１２で検出し、ラマン散乱光をラマン散乱光検出手段１３で検出するようにする。これにより、観察対象の標本を動かすことなく、ラマン散乱光検出およびＣＡＲＳ光観察を選択的に行うことができ、煩雑な作業を要することなく、ＣＡＲＳ光観察のための振動周波数を効率的に選択できる。
Abstract translation: 通过激光照射光学系统（3,4,5和6）将样品与CARS激光束和拉曼散射激光束同轴照射，使得CARS光被CARS光检测装置（12）检测，而 由拉曼散射光检测装置（13）检测拉曼散射光。 因此，在不移动要观察的样本的情况下，可以选择性地进行拉曼散射光检测和CARS光观察，从而可以有效地选择CARS光观测的振动频率，而不需要任何复杂的工作。
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公开(公告)号：WO2010055362A3
公开(公告)日：2010-07-08
申请号：PCT/HU2009000095
申请日：2009-11-17
Applicant: FEMTONICS KFT , ROZSA BAIAZS , KATONA GERGELY , VIZI E SZILVESZTER
Inventor： ROZSA BAIAZS , KATONA GERGELY , VIZI E SZILVESZTER
CPC classification number: G01N21/6458 , G01N21/6428 , G01N2201/105 , G02B21/002 , G02B21/0036 , G02B26/10 , G06K9/00134 , G06K9/209 , G06K9/30
Abstract: The invention relates to a method for scanning a region of interest, such as a portion of a neural process, via a laser scanning microscope having focusing means for focusing a laser beam and having electro-mechano-optic deflecting means for deflecting the laser beam, the method comprising: providing a primary scanning trajectory (92a) for the at least one region of interest; providing a plurality of spaced apart (89) auxiliary scanning trajectories (92b) running along the primary scanning trajectory within the region of interest; providing a scanning sequence for scanning the scanning trajectories (192); providing cross-over (94) trajectories between the scanning trajectories of two consecutive scanning trajectories in the scanning sequence. The invention further relates to a measuring system for implementing the method according to the invention.
Abstract translation: 本发明涉及一种用于通过激光扫描显微镜扫描诸如神经过程的一部分的感兴趣区域的方法，所述激光扫描显微镜具有用于聚焦激光束并具有用于偏转激光束的电机械光学偏转装置的聚焦装置， 所述方法包括：为所述至少一个感兴趣区域提供主扫描轨迹（92a）; 提供沿所述感兴趣区域内的主扫描轨迹延伸的多个间隔开的（89）辅助扫描轨迹（92b） 提供用于扫描扫描轨迹（192）的扫描序列; 在扫描序列中的两个连续扫描轨迹的扫描轨迹之间提供交叉（94）轨迹。 本发明还涉及一种用于实现根据本发明的方法的测量系统。
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公开(公告)号：WO2010048584A2
公开(公告)日：2010-04-29
申请号：PCT/US2009061957
申请日：2009-10-23
Applicant: APERIO TECHNOLOGIES INC , NAJMABADI PEYMAN , CRANDALL GREGORY J , STEARRETT AARON ALAN , SOENKSEN DIRK G , LEE CHRISTOPHER ADAM , PERZ CYNTHIA
Inventor： NAJMABADI PEYMAN , CRANDALL GREGORY J , STEARRETT AARON ALAN , SOENKSEN DIRK G , LEE CHRISTOPHER ADAM , PERZ CYNTHIA
CPC classification number: G02B21/0084 , G01N21/64 , G01N21/6458 , G01N2021/6471 , G02B21/00 , G02B21/002 , G02B21/0076 , G02B21/16 , G06K9/00134 , G06T7/30 , G06T2207/10056 , G06T2207/10064 , G06T2207/30024 , H04N5/3692
Abstract: A whole slide fluorescence digital pathology system is provided that uses a monochrome TDI line scan camera, which is particularly useful in fluorescence scanning where the signal is typically much weaker than in brightfield microscopy. The system uses oblique brightfield illumination for fast and accurate tissue finding and employs a unique double sweep focus scoring and objective lens height averaging technique to identify focus points and create a focus map that can be followed during subsequent scanning to provide autofocusing capability. The system also scans and analyzes image data to determine the optimal line rate for the TDI line scan camera to use during subsequent scanning of the digital slide image and it also creates a light profile to compensate for loss of illumination light due to roll off.
Abstract translation: 提供了一个完整的幻灯片荧光数字病理系统，该系统使用单色TDI线扫描照相机，这在荧光扫描中特别有用，其中信号通常比明视野显微镜更弱。 该系统使用倾斜的明场照明进行快速准确的组织发现，并采用独特的双重扫描聚焦评分和物镜高度平均技术来确定聚焦点并创建聚焦图，在随后的扫描过程中可以遵循该聚焦图提供自动聚焦功能。 该系统还扫描和分析图像数据，以确定TDI线扫描相机在随后的数字载玻片图像扫描期间使用的最佳线速率，并且还创建光轮廓以补偿由于滚降导致的照明光损失。
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