摘要:
A method for producing a clean lactase is described. The method comprises incubating a preparation comprising said lactase at a pH close to the isoelectric point of said lactase.
摘要:
본 발명은 고당전이 활성을 갖으며, 카르복시 말단부가 절단된, 베타-갈라토시다제 효소의 변이체 및 그 용도에 관한 것이다. 보다 상세하게는, 카르복시 말단부가 절단된 베타-갈락토시다제 변이체, 이를 코딩하는 유전자, 상기 유전자를 함유하는 재조합 벡터, 상기 유전자 또는 재조합 벡터로 형질 전환된 재조합 미생물 및 상기 재조합 미생물을 이용한 베타-갈락토시다제 변이체의 제조방법에 관한 것이다. 본 발명에 따른 카르복시 말단부가 절단된 베타-갈락토시다제 변이체의 높은 당전이 활성을 이용하여, 효율적으로 갈락토올리고당을 대량 생산할 수 있다.
摘要:
Aspects of the invention relate to recombinant adeno-associated viruses (rAAVs). In some aspects, the rAAVs comprise artificial genetic regulatory elements that modulate transgene expression. In some aspects, the disclosure relates to the treatment of lysosomal storage disorders.
摘要:
A spray-dried composition comprising an enzyme which is a β-galactosidase having transgalactosyiating activity and a maltodextrin and/or sodium chloride.
摘要:
The present disclosure provides, among other things, methods and compositions for detecting and/or quantifying analytes using fragment complementation technologies. In accordance with various embodiments of the present disclosure, a kit can include a) a capture probe immobilized on a surface, wherein the capture probe can associate with an analyte in a sample, thereby forming at least one captured analyte; b) a detection element including a target interacting probe associated with a first subunit of a detectable entity, wherein the target interacting probe can associate with the captured analyte so that a first complex is formed; and c) a second subunit that can complement the first subunit and generate a detectable entity, wherein the presence and/or amount of the analyte is indicated by detecting level or activity of the detectable entity.
摘要:
The present invention relates to a method of obtaining a non-acidic human milk oligosaccharide (HMO) or a precursor in crystalline form from an aqueous medium, such as a fermentation broth, by drying the aqueous medium and then treating it with a hot C 1 -C 6 alcohol solvent.
摘要:
There is provided a novel cold-active beta-galactosidase enzyme specific for lactose. The enzyme is thus useful in e.g. the food industry for catalyzing at low temperatures the hydrolysis of lactose disaccharide into its constituent monosaccharides, glucose and galactose. The present invention further provides a method of producing the cold-active beta-galactosidase by recombinant DNA technology.