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1.发明申请METHOD FOR PRODUCING A PROTEIN HYDROLYSATE EMPLOYING AN ASPERGILLUS FUMIGATUS TRIPEPTIDYL PEPTIDASE 审中-公开生产蛋白水解产物的方法，其使用ASPERGILLUS FUMIGATUS三磷酸化肽酶
公开(公告)号：WO2017147060A9
公开(公告)日：2017-08-31
申请号：PCT/US2017/018686
申请日：2017-02-21
发明人： KRAGH, Karsten Matthias , HAANING, Svend , MEINJOHANNS, Ernst , DEGN, Peter Edvard , BAK, Steffen Yde , EISELE, Thomas
摘要： The present invention relates to compositions and methods for the production of a hydrolysate comprising at least one endoprotease and a tripeptidyl peptidase capable of cleaving tripeptides from the N-terminus a peptide and/or proteins having one or more of lysine, arginine or glycine in the P1 position wherein said tripeptidyl peptidase is capable of being used at a temperature between 45ºC and 70ºC.
摘要翻译： 本发明涉及用于生产水解产物的组合物和方法，所述水解产物包含至少一种内切蛋白酶和能够从N端切割三肽的三肽基肽酶和/或具有一个或多个 在P1位置的赖氨酸，精氨酸或甘氨酸，其中所述三肽基肽酶能够在45℃和70℃之间的温度下使用; C。
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公开(公告)号：WO2016210395A8
公开(公告)日：2016-12-29
申请号：PCT/US2016/039494
申请日：2016-06-27
发明人： KRAGH, Karsten Matthias , HAANING, Svend , MEINJOHANNS, Ernst , EISELE, Thomas , DEGN, Peter Edvard , BAK, Steffen Yde
摘要： The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. In some embodiments, the present disclosure further provides to methods of obtaining protein hydrolysates useful as flavor improving agents.
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公开(公告)号：WO2016164096A1
公开(公告)日：2016-10-13
申请号：PCT/US2016/015299
申请日：2016-01-28
CPC分类号： A23C9/1275 , A23C9/1209 , A23C9/123 , A23C9/1234 , A23C9/1238 , A23C9/1307 , A23C9/137 , A23C9/1422 , A23C13/16 , C12Y304/17 , C12Y304/21 , C12Y304/24
摘要： The present disclosure provides compositions, methods, and uses concerning the preparation of a fermented milk product having a high protein content such as high protein yogurt, Greek yogurt, Labneh or sour cream comprising application of a microorganism and an exogenous protease to a milk substrate having a protein content of at least or greater than 6.5%. Application of proteases of the classes EC 3.4.21 (serine endopeptidases), EC 3.4.24 (metalloendopeptidases) and EC 3.4.17 (metallocarboxypeptidases) is envisaged. Especially application of neutrase (Protex 7L) and Protex 6L resulted in a decreased viscosity and a good flavour.
摘要翻译： 本公开提供了关于制备具有高蛋白质含量的发酵乳产品的组合物，方法和用途，例如高蛋白酸奶，希腊酸奶，Labneh或酸奶油，其包括将微生物和外源蛋白酶施用于具有 蛋白质含量至少为6.5％以上。 考虑到EC 3.4.21（丝氨酸内肽酶），EC 3.4.24（金属内肽酶）和EC 3.4.17（金属羧肽酶）的蛋白酶的应用。 尤其是中性粒子（Protex 7L）和Protex 6L的应用导致粘度降低和味道好。
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公开(公告)号：WO2016210395A1
公开(公告)日：2016-12-29
申请号：PCT/US2016/039494
申请日：2016-06-27
发明人： KRAGH, Karsten Matthias , HAANING, Svend , MEINJOHANNS, Ernst , EISELE, Thomas , DEGN, Peter Edvard , BAK, Steffen Yde
CPC分类号： C12N9/485 , C07K2319/02 , C12N15/80 , C12P21/06 , C12Y304/11
摘要： The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. In some embodiments, the present disclosure further provides to methods of obtaining protein hydrolysates useful as flavor improving agents.
摘要翻译： 本公开提供了具有氨基肽酶活性的多肽和分离的编码多肽的核酸序列。 在一些实施方案中，本公开还提供了包含核酸序列的核酸构建体，载体和宿主细胞以及用于产生多肽的方法。 在一些实施方案中，本公开进一步提供了获得可用作风味改善剂的蛋白质水解产物的方法。
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5.发明申请METHOD OF GENERATING A SACCHARIDE CONTAINING A GALACTOSE AND A FRUCTOSE MOIETY EMPLOYING ENZYME WITH TRANSGALACTOSYLATING ACTIVITY 审中-公开产生含有胶原糖的壳聚糖和使用转移活性的酶解纤维素酶的方法
公开(公告)号：WO2016071502A1
公开(公告)日：2016-05-12
申请号：PCT/EP2015/075948
申请日：2015-11-06
IPC分类号： C12P19/18 , C12P19/12 , C12P19/04 , C12P19/44 , A23L33/21 , A23C9/12 , A23C9/20 , C13K13/00 , C07H3/04 , C12N9/38
CPC分类号： A23C9/1206 , A23C9/203 , A23L33/21 , C07H1/00 , C07H3/04 , C07H3/06 , C12N9/2471 , C12P19/04 , C12P19/12 , C12P19/18 , C12Y302/01023 , C13K13/00 , C13K13/005
摘要： A method of generating a saccharide, especially lactulose or lactosucrose, in the presence of a transgalactosylase enzyme, is described herein.
摘要翻译： 本文描述了在转半乳糖酶的存在下产生糖，特别是乳果糖或乳糖的方法。
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公开(公告)号：WO2016123326A1
公开(公告)日：2016-08-04
申请号：PCT/US2016/015337
申请日：2016-01-28
CPC分类号： A23C9/1209 , A23C9/1206 , A23C9/123 , A23C9/127 , A23C13/16 , A23C17/02 , C12Y302/01 , C12Y304/17 , C12Y304/21 , C12Y304/24
摘要： The present invention relates to a method of preparing a fermented milk product. The method comprises the steps of treating a milk substrate with a low pH sensitive peptidase and a microorganism, and allowing the treated milk substrate to ferment to produce the fermented milk product.
摘要翻译： 本发明涉及一种制备发酵乳制品的方法。 该方法包括以下步骤：用低pH敏感性肽酶和微生物处理乳底物，并使经过处理的乳基质发酵以产生发酵乳制品。
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公开(公告)号：WO2016062855A1
公开(公告)日：2016-04-28
申请号：PCT/EP2015/074600
申请日：2015-10-23
发明人： KRAGH, Karsten Matthias , MEINJOHANNS, Ernst , MIASNIKOV, Andrei , MA, Maria , EISELE, Thomas , HAANING, Svend , DEGN, Peter Edvard , BAK, Steffen Yde
CPC分类号： A23J3/34 , A23K10/14 , A23K20/147 , A23K50/30 , A23K50/75 , C12N9/485 , C12P21/06 , C12Y304/14 , C12Y304/14009
摘要： A method for the production of a hydrolysate comprising: (a) admixing at least one protein or a portion thereof with: (A) at least one endoprotease; and (B) (a') at least one proline tolerant tripeptidyl peptidase or fermentate comprising a proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at P1; and synthetic amino acids at P1; or (b') at least one proline tolerant tripeptidyl peptidase having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at PV; and synthetic amino acids at PV; and (b) recovering the hydrolysate. The invention also relates to methods for producing a hydrolysate comprising the use of an endoprotease an exo-tripeptidyl peptidase of the S53 family and an aminopeptidase, to uses of a proline tolerant tripeptidyl peptidase, compositions, food and/or feed additive compositions comprising the same, as well as hydrolysates and uses of proline tolerant tripeptidyl peptidases.
摘要翻译： 一种生产水解产物的方法，包括：（a）将至少一种蛋白质或其部分与下列物质混合：（A）至少一种内切蛋白酶; 和（B）（a'）至少一种脯氨酸耐受性三肽基肽酶或发酵物，其包含主要具有外肽酶活性的脯氨酸耐受性三肽基肽酶，其中所述脯氨酸耐受性三肽基肽酶能够从具有以下的肽的N-末端切割三肽： 在P1; 和P1的合成氨基酸; 或（b'）至少一种具有外肽酶活性的脯氨酸耐受性三肽基肽酶，其中所述脯氨酸耐受性三肽基肽酶能够从肽的N-末端切割三肽：在PV下具有脯氨酸; 和PV处的合成氨基酸; 和（b）回收水解产物。 本发明还涉及用于生产水解产物的方法，其包括使用内切蛋白酶，S53家族的外切三肽基肽酶和氨基肽酶，使用脯氨酸耐受性三肽基肽酶，组合物，包含其的食品和/或饲料添加剂组合物 ，以及脯氨酸耐受性三肽基肽酶的水解产物和用途。
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8.发明申请A METHOD FOR PREPARING A DAIRY PRODUCT HAVING A STABLE CONTENT OF GALACTO-OLIGOSACCHARIDE(S) 审中-公开一种用于制备具有稳定含量的寡糖的乳制品的方法（S）
公开(公告)号：WO2015086746A1
公开(公告)日：2015-06-18
申请号：PCT/EP2014/077380
申请日：2014-12-11
CPC分类号： C12P19/14 , A23C9/1206 , A23C9/1307 , A23C2220/202 , A23L33/135 , A23V2002/00 , A23Y2300/25 , A61K31/702 , C12Y302/01023
摘要： The present invention describes a process for in situ generation of stable GOS in different dairy products by the use of a transgalactosylating β-galactosidase.
摘要翻译： 本发明描述了通过使用反式半乳糖基化β-半乳糖苷酶在不同乳制品中原位产生稳定GOS的方法。
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9.发明申请
公开(公告)号：WO2017147060A1
公开(公告)日：2017-08-31
申请号：PCT/US2017/018686
申请日：2017-02-21
发明人： KRAGH, Karsten Matthias , HAANING, Svend , MEINJOHANNS, Ernst , DEGN, Peter Edvard , BAK, Steffen Yde , EISELE, Thomas
CPC分类号： C12P21/06 , A23J3/341 , A23J3/346 , A23K10/10 , A23K10/14 , A23K20/147 , A23K20/189 , A23K50/30 , A23K50/60 , A23K50/75 , C12N9/485 , C12Y304/14009 , C12Y304/1401
摘要： The present invention relates to compositions and methods for the production of a hydrolysate comprising at least one endoprotease and a tripeptidyl peptidase capable of cleaving tripeptides from the N-terminus a peptide and/or proteins having one or more of lysine, arginine or glycine in the P1 position wherein said tripeptidyl peptidase is capable of being used at a temperature between 45ºC and 70ºC.
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10.发明申请RECOMBINANT HOST CELL EXPRESSING BETA-GALACTOSIDASE AND/OR TRANSGALACTOSYLATING ACTIVITY DEFICIENT IN MANNANASE, CELLULASE AND PECTINASE 审中-公开在MANNANASE，CELLULASE和PECTINASE中表达β-GALACTOSIDASE和/或转移活性缺陷的重组宿主细胞
公开(公告)号：WO2016071504A1
公开(公告)日：2016-05-12
申请号：PCT/EP2015/075950
申请日：2015-11-06
发明人： LARSEN, Morten Krog , CRAMER, Jacob Flyvholm , LABARGE, Jeremy , EISELE, Thomas , HANSEN KJER, Karina
IPC分类号： A23C9/12 , A23C9/137 , A23L29/206 , A23L29/231 , A23L29/238 , A23L29/244 , A23L29/25 , A23L29/262 , A23L33/135
CPC分类号： A23L33/21 , A23C9/1206 , A23C9/1216 , A23C2220/202 , A23G9/38 , A23L33/135 , C12N9/2471 , C12Y302/01023
摘要： A host cell capable of expressing a polypeptide having β-galactosidase and/or transgalactosylating activity and which host cell is modified to be cellulase, mannanase and pectinase deficient.
摘要翻译： 能够表达具有β-半乳糖苷酶和/或转半乳糖基化活性的多肽的宿主细胞，宿主细胞被修饰为纤维素酶，甘露聚糖酶和果胶酶缺陷型。
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