-
1.发明申请
公开(公告)号：WO2011040971A9
公开(公告)日：2011-04-07
申请号：PCT/US2010/002659
申请日：2010-09-30
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , CLARK, Sonya , BIBILLO, Arek , PELUSO, Paul , CHRISTIANS, Fred , HE, Molly , PARK, Insil , LEE, Harold , BJORNSON, Keith , JIA, Lei , EMIG, Robin
Inventor： CLARK, Sonya , BIBILLO, Arek , PELUSO, Paul , CHRISTIANS, Fred , HE, Molly , PARK, Insil , LEE, Harold , BJORNSON, Keith , JIA, Lei , EMIG, Robin
Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
-
2.发明申请GENERATION OF MODIFIED POLYMERASES FOR IMPROVED ACCURACY IN SINGLE MOLECULE SEQUENCING 审中-公开在单分子序列中改进的改进聚合物的生成
公开(公告)号：WO2011040971A2
公开(公告)日：2011-04-07
申请号：PCT/US2010002659
申请日：2010-09-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
CPC classification number: C12N9/1252 , C12Q1/6869 , C12Q2521/101
Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
Abstract translation: 提供了包含重组DNA聚合酶的组合物，其包括氨基酸取代，插入，缺失和/或异源或外源特征，其在聚合酶上赋予改进的性质以用于增强的单分子测序。 这种性质可以包括聚合酶动力学循环的一个或多个步骤的降低的反应速率，增加的闭合聚合酶/ DNA复合物稳定性，增强的金属离子配位，减少的核酸外切酶活性，降低的支化部分等。 还提供了编码具有上述表型的聚合酶的核酸，以及使用这种聚合酶制备DNA或顺序DNA模板的方法。
-
3.发明申请
公开(公告)号：WO2011040971A3
公开(公告)日：2011-11-03
申请号：PCT/US2010002659
申请日：2010-09-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
CPC classification number: C12N9/1252 , C12Q1/6869 , C12Q2521/101
Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
-
4.发明申请
公开(公告)号：WO2009145820A3
公开(公告)日：2009-12-03
申请号：PCT/US2009/001974
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , CLARK, Sonya , BIBILLO, Arek , PELUSO, Paul , CHRISTIANS, Fred , HE, Molly , PARK, Insil , LEE, Harold , BJORNSON, Keith , JIA, Lei , EMIG, Robin
Inventor： CLARK, Sonya , BIBILLO, Arek , PELUSO, Paul , CHRISTIANS, Fred , HE, Molly , PARK, Insil , LEE, Harold , BJORNSON, Keith , JIA, Lei , EMIG, Robin
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
-
5.发明申请GENERATION OF MODIFIED POLYMERASES FOR IMPROVED ACCURACY IN SINGLE MOLECULE SEQUENCING 审中-公开在单分子序列中改进的改进聚合物的生成
公开(公告)号：WO2009145820A2
公开(公告)日：2009-12-03
申请号：PCT/US2009001974
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
CPC classification number: C12N9/1252 , C12Q1/6844 , C12Q1/6869 , C12Q2521/101
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
Abstract translation: 提供了包含修饰的重组聚合酶的组合物，其表现出小于其衍生自其的聚合酶的支化部分的支化部分，或对于磷酸酯标记的核苷酸类似物的支化部分小于约25％。 还提供了包含修饰的重组聚合酶的组合物，其表现出相对于亲本聚合酶具有增加的稳定性的闭合的聚合酶/ DNA复合物。 还提供了包含修饰的重组聚合酶的组合物，其相对于亲本聚合酶表现出降低的速率常数。 提供了产生具有上述表型的聚合酶的方法。 提供使用这种聚合酶制备DNA或顺序DNA模板的方法。
-
Patent Agency Ranking
公开(公告)号：WO2007041342A3
公开(公告)日：2007-10-04
申请号：PCT/US2006038133
申请日：2006-09-28
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , XU YUE , WEGENER JEFFEY , BIBILLO AREK
Inventor： XU YUE , WEGENER JEFFEY , BIBILLO AREK
CPC classification number: C07H19/10 , C07H21/00 , C12Q1/6869 , C12Q2533/101 , C12Q2525/117 , C12Q2525/101
Abstract: Labeled nucleotide analogs used in place of naturally occurring nucleoside triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions, and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays and others.
Abstract translation: 在模板定向核酸合成反应和其他核酸反应中使用代替天然存在的核苷三磷酸或其他类似物的标记的核苷酸类似物，以及基于其的各种分析，包括DNA测序，单碱基鉴定，杂交测定等。
-
公开(公告)号：WO2009145828A3
公开(公告)日：2009-12-03
申请号：PCT/US2009/002003
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , BJORNSON, Keith , LEE, Harold , KORLACH, Jonas , TURNER, Stephen , BIBILLO, Arek , CHRISTIANS, Fred , EMIG, Robin , HE, Molly , PARK, Insil , JIA, Lei , FEDOROV, Andrei , LYLE, John
Inventor： BJORNSON, Keith , LEE, Harold , KORLACH, Jonas , TURNER, Stephen , BIBILLO, Arek , CHRISTIANS, Fred , EMIG, Robin , HE, Molly , PARK, Insil , JIA, Lei , FEDOROV, Andrei , LYLE, John
Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
-
公开(公告)号：WO2008051530A3
公开(公告)日：2008-12-18
申请号：PCT/US2007022459
申请日：2007-10-22
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , RANK DAVID R , BIBILLO AREK , HANZEL DAVID K , PELUSO PAUL , WEGENER JEFF
Inventor： RANK DAVID R , BIBILLO AREK , HANZEL DAVID K , PELUSO PAUL , WEGENER JEFF
CPC classification number: C12N9/1252 , C12P19/34
Abstract: Compositions that include DNA polymerases having increased residence times for nucleotide analogues, particularly modified recombinant F29-type DNA polymerases with such increased residence times, are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA amplification are also provided. Compositions including a-thiophosphate nucleotide analogues with four or more phosphate groups are described, as are methods for determining the sequence of nucleic acid molecules using such analogues.
Abstract translation: 提供了包括具有增加的核苷酸类似物停留时间的DNA聚合酶的组合物，特别是具有增加的停留时间的修饰的重组F29-型DNA聚合酶。 还提供了在测序和DNA扩增中制备聚合酶和使用聚合酶的方法。 描述了包含具有四个或更多个磷酸基团的α-硫代磷酸核苷酸类似物的组合物，以及使用此类类似物确定核酸分子序列的方法。
-
公开(公告)号：WO2008051530A2
公开(公告)日：2008-05-02
申请号：PCT/US2007/022459
申请日：2007-10-22
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , RANK, David, R. , BIBILLO, Arek , HANZEL, David, K. , PELUSO, Paul , WEGENER, Jeff
Inventor： RANK, David, R. , BIBILLO, Arek , HANZEL, David, K. , PELUSO, Paul , WEGENER, Jeff
CPC classification number: C12N9/1252 , C12P19/34
Abstract: Compositions that include DNA polymerases having increased residence times for nucleotide analogues, particularly modified recombinant Φ29-type DNA polymerases with such increased residence times, are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA amplification are also provided. Compositions including α-thiophosphate nucleotide analogues with four or more phosphate groups are described, as are methods for determining the sequence of nucleic acid molecules using such analogues.
Abstract translation: 提供了包含具有增加的核苷酸类似物停留时间的DNA聚合酶的组合物，特别是具有增加的停留时间的经修饰的重组Φ29型DNA聚合酶。 还提供了在测序和DNA扩增中制备聚合酶和使用聚合酶的方法。 描述了包含具有四个或更多个磷酸基团的α-硫代磷酸核苷酸类似物的组合物，以及使用此类类似物确定核酸分子序列的方法。
-
公开(公告)号：WO2007041342A2
公开(公告)日：2007-04-12
申请号：PCT/US2006/038133
申请日：2006-09-28
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , XU, Yue , WEGENER, Jeffey , BIBILLO, Arek
Inventor： XU, Yue , WEGENER, Jeffey , BIBILLO, Arek
CPC classification number: C07H19/10 , C07H21/00 , C12Q1/6869 , C12Q2533/101 , C12Q2525/117 , C12Q2525/101
Abstract: Labeled nucleotide analogs used in place of naturally occurring nucleoside triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions, and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays and others.
Abstract translation: 在模板指导的核酸合成反应和其他核酸反应中用于代替天然存在的三磷酸核苷或其它类似物的标记的核苷酸类似物，以及基于其的各种分析，包括DNA测序，单碱基鉴定，杂交 化验和其他。
-
-
-
-
-
-
-
-
-
Search Results
15
records
(took 0.012 seconds)
