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公开(公告)号：WO2009145828A3
公开(公告)日：2009-12-03
申请号：PCT/US2009/002003
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , BJORNSON, Keith , LEE, Harold , KORLACH, Jonas , TURNER, Stephen , BIBILLO, Arek , CHRISTIANS, Fred , EMIG, Robin , HE, Molly , PARK, Insil , JIA, Lei , FEDOROV, Andrei , LYLE, John
Inventor： BJORNSON, Keith , LEE, Harold , KORLACH, Jonas , TURNER, Stephen , BIBILLO, Arek , CHRISTIANS, Fred , EMIG, Robin , HE, Molly , PARK, Insil , JIA, Lei , FEDOROV, Andrei , LYLE, John
Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
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公开(公告)号：WO2009145818A1
公开(公告)日：2009-12-03
申请号：PCT/US2009/001970
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC , EID, John , TURNER, Stephen , DALAL, Ravi , FLUSBERG, Benjamin , KORLACH, Jonas , LIN, Steven , FEHR, Adrian , CHRISTIANS, Fred , EMIG, Robin
Inventor： EID, John , TURNER, Stephen , DALAL, Ravi , FLUSBERG, Benjamin , KORLACH, Jonas , LIN, Steven , FEHR, Adrian , CHRISTIANS, Fred , EMIG, Robin
CPC classification number: B01J19/0046 , B01J2219/00596 , C12N2310/50 , C12N2310/51 , C12Q1/6869 , C12Q2535/122 , C40B40/06 , G01N33/54313 , G01N33/54366
Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.
Abstract translation: 提供了将单一分析物分子非随机加载到阵列结构中的方法，组合物和阵列。
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公开(公告)号：WO2009100382A1
公开(公告)日：2009-08-13
申请号：PCT/US2009/033467
申请日：2009-02-06
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , OTTO, Geoffrey , SHEN, Gene , KONG, Xiangxu , EMIG, Robin
Inventor： OTTO, Geoffrey , SHEN, Gene , KONG, Xiangxu , EMIG, Robin
IPC: C07H21/00
Abstract: The present invention provides methods and compositions for performing illuminated reactions, particularly sequencing reactions, while mitigating and/or preventing photodamage to reactants that can result from prolonged illumination. In particular, the invention provides methods and compositions for incorporating photoprotective agents into conjugates comprising reporter molecules and nucleoside polyphosphates.
Abstract translation: 本发明提供用于进行照明反应，特别是测序反应的方法和组合物，同时减轻和/或防止可能由长时间照射引起的反应物的光损伤。 特别地，本发明提供了将光保护剂掺入包含报道分子和核苷多磷酸盐的缀合物中的方法和组合物。
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4.发明申请
公开(公告)号：WO2003075129A2
公开(公告)日：2003-09-12
申请号：PCT/US2003/006551
申请日：2003-03-03
Applicant: MAXYGEN, INC. , GUSTAFSSON, Claes , GOVINDARAJAN, Sridar , EMIG, Robin , FOX, Richard, John , ROY, Ajoy , MINSHULL, Jeremy , DAVIS, S., Christopher , COX, Anthony , PATTEN, Phil , CASTLE, Linda, A. , SIEHL, Daniel, L. , GORTON, Rebecca, Lynne , CHEN, Teddy
Inventor： GUSTAFSSON, Claes , GOVINDARAJAN, Sridar , EMIG, Robin , FOX, Richard, John , ROY, Ajoy , MINSHULL, Jeremy , DAVIS, S., Christopher , COX, Anthony , PATTEN, Phil , CASTLE, Linda, A. , SIEHL, Daniel, L. , GORTON, Rebecca, Lynne , CHEN, Teddy
IPC: G06F
Abstract: The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.
Abstract translation: 本发明一般涉及快速有效地搜索生物相关数据空间的方法。 更具体地，本发明包括从复杂的生物分子文库或这些文库集合鉴定具有所需性质或最适合于获得这些性质的生物分子的方法。 本发明还提供了对序列活性关系建模的方法。 由于许多方法是计算机实现的，本发明另外提供用于执行这些方法的数字系统和软件。
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5.发明申请
公开(公告)号：WO2011040971A9
公开(公告)日：2011-04-07
申请号：PCT/US2010/002659
申请日：2010-09-30
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , CLARK, Sonya , BIBILLO, Arek , PELUSO, Paul , CHRISTIANS, Fred , HE, Molly , PARK, Insil , LEE, Harold , BJORNSON, Keith , JIA, Lei , EMIG, Robin
Inventor： CLARK, Sonya , BIBILLO, Arek , PELUSO, Paul , CHRISTIANS, Fred , HE, Molly , PARK, Insil , LEE, Harold , BJORNSON, Keith , JIA, Lei , EMIG, Robin
Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
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Patent Agency Ranking6.发明申请GENERATION OF MODIFIED POLYMERASES FOR IMPROVED ACCURACY IN SINGLE MOLECULE SEQUENCING 审中-公开在单分子序列中改进的改进聚合物的生成
公开(公告)号：WO2011040971A2
公开(公告)日：2011-04-07
申请号：PCT/US2010002659
申请日：2010-09-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
CPC classification number: C12N9/1252 , C12Q1/6869 , C12Q2521/101
Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
Abstract translation: 提供了包含重组DNA聚合酶的组合物，其包括氨基酸取代，插入，缺失和/或异源或外源特征，其在聚合酶上赋予改进的性质以用于增强的单分子测序。 这种性质可以包括聚合酶动力学循环的一个或多个步骤的降低的反应速率，增加的闭合聚合酶/ DNA复合物稳定性，增强的金属离子配位，减少的核酸外切酶活性，降低的支化部分等。 还提供了编码具有上述表型的聚合酶的核酸，以及使用这种聚合酶制备DNA或顺序DNA模板的方法。
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7.发明申请
公开(公告)号：WO0151663A3
公开(公告)日：2002-06-13
申请号：PCT/US0101056
申请日：2001-01-10
Applicant: MAXYGEN INC , BASS STEVEN H , DAVIS S CHRISTOPHER , PATTEN PHILLIP A , TOBIN MATTHEW , MINSHULL JEREMY , WELCH MARK , GUSTAFSSON CLAES , CARR BRIAN , JENNE STEPHANE , RAILLARD SUN AI , CRAMERI ANDREAS , STEMMER WILLEM P C , EMIG ROBIN , LONGCHAMP PASCAL , GOLDMAN STANLEY , GIVER LORRAINE J , AFFHOLTER JOSEPH A
Inventor： BASS STEVEN H , DAVIS S CHRISTOPHER , PATTEN PHILLIP A , TOBIN MATTHEW , MINSHULL JEREMY , WELCH MARK , GUSTAFSSON CLAES , CARR BRIAN , JENNE STEPHANE , RAILLARD SUN AI , CRAMERI ANDREAS , STEMMER WILLEM P C , EMIG ROBIN , LONGCHAMP PASCAL , GOLDMAN STANLEY , GIVER LORRAINE J , AFFHOLTER JOSEPH A
CPC classification number: C12N15/1031 , B01J19/0046 , B01J2219/00378 , B01J2219/00585 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00695 , B01J2219/007 , B01J2219/00722 , B82Y30/00 , C12N15/1027 , C40B60/04 , G06F19/18 , Y02A90/22
Abstract: Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.
Abstract translation: 提供了用于多样化生成和筛选的集成系统和方法。 该系统使用普通液体和阵列处理组分来提供核酸多样化，转录，翻译，产物筛选和随后的多样化反应。
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公开(公告)号：WO2010117470A3
公开(公告)日：2011-03-31
申请号：PCT/US2010001072
申请日：2010-04-09
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , TURNER STEPHEN , FLUSBERG BENJAMIN , FOQUET MATHIEU , CALLEBAUT HANS , SEBRA ROBERT , CHAUDHURI BIDHAN , SORENSON JON , BJORNSON KEITH , FEHR ADRIAN , KORLACH JONAS , EMIG ROBIN
Inventor： TURNER STEPHEN , FLUSBERG BENJAMIN , FOQUET MATHIEU , CALLEBAUT HANS , SEBRA ROBERT , CHAUDHURI BIDHAN , SORENSON JON , BJORNSON KEITH , FEHR ADRIAN , KORLACH JONAS , EMIG ROBIN
CPC classification number: G01N33/48721 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , C12Q1/6874 , C12Q2565/631 , G01N27/447 , G01N27/44791 , C12Q2563/116 , C12Q2527/113
Abstract: The invention relates to devices and methods for nanopore sequencing. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed.
Abstract translation: 本发明涉及纳米孔测序的装置和方法。 本发明包括纳入电子电路的纳米孔阵列，例如在CMOS中。 在一些情况下，纳米孔阵列包括用于隔离电子信号的电阻开口以改善测序。 公开了通过纳米孔控制易位的方法。
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公开(公告)号：WO2010036311A3
公开(公告)日：2010-06-17
申请号：PCT/US2009005169
申请日：2009-09-16
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , MAXHAM KENNETH MARK , SORENSON JON , EID JOHN , MARKS PATRICK , TRAVERS KEVIN , GRAY DONALD , EMIG ROBIN , CHAISSON MARK , FLUSBERG BENJAMIN
Inventor： MAXHAM KENNETH MARK , SORENSON JON , EID JOHN , MARKS PATRICK , TRAVERS KEVIN , GRAY DONALD , EMIG ROBIN , CHAISSON MARK , FLUSBERG BENJAMIN
IPC: G01N21/00
CPC classification number: C12Q1/6869 , G01N2021/7786 , Y10T436/143333 , C12Q2525/301 , C12Q2533/101 , C12Q2537/149 , C12Q2565/631 , C12Q2525/307 , C12Q2525/191
Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.
Abstract translation: 提供了用于在分析反应期间执行间歇检测的方法，装置和系统。 这种方法便于从不同的反应时间收集反应数据。 此外，此类方法可用于减少在给定反应时间下照明分析反应中一种或多种反应物的光致损伤。 在优选的实施方案中，使反应混合物经历至少一个照射和未照射的时间段并使其进行，使得照射反应混合物的时间小于光诱导的损伤阈值时间段。
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公开(公告)号：WO2009145828A2
公开(公告)日：2009-12-03
申请号：PCT/US2009002003
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , BJORNSON KEITH , LEE HAROLD , KORLACH JONAS , TURNER STEPHEN , BIBILLO AREK , CHRISTIANS FRED , EMIG ROBIN , HE MOLLY , PARK INSIL , JIA LEI , FEDOROV ANDREI , LYLE JOHN
Inventor： BJORNSON KEITH , LEE HAROLD , KORLACH JONAS , TURNER STEPHEN , BIBILLO AREK , CHRISTIANS FRED , EMIG ROBIN , HE MOLLY , PARK INSIL , JIA LEI , FEDOROV ANDREI , LYLE JOHN
CPC classification number: C12Q1/6869 , C12Q1/6874 , C12Q2527/125 , C12Q2527/119 , C12Q2527/101 , C12Q2565/1015 , C12Q2527/137 , C12Q2521/101
Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
Abstract translation: 用于核苷酸测序的组合物，试剂盒，方法和系统，其包括产生聚合酶反应，其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶，包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。
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