公开(公告)号：WO2011002731A3

公开(公告)日：2011-01-06

申请号：PCT/US2010/040285

申请日：2010-06-29

Applicant: THE WAY OF H, INC. ,  LEE, Harold ,  DANNENBERG, Roger

Inventor： LEE, Harold ,  DANNENBERG, Roger

IPC: G10H7/00 ,  G10H3/18 ,  G10H1/34

Abstract: A method includes receiving a user selection of a musical piece; providing performance cues to a user to perform musical events on a musical instrument, wherein the performance cues are synchronized to expert performance data of the musical piece; receiving audio data corresponding to musical events performed by the user on the musical instrument; detecting fundamental frequencies associated with the user-performed musical events; determining an extent to which the user-performed musical events have been correctly or incorrectly performed; providing real-time or near real-time audio feedback and/or visual feedback indicating the extent to which the user-performed musical events have been correctly or incorrectly performed; and using the expert performance data as real-time or near real-time audio and/or video feedback by controlling an output level of the expert performance data output to the user during a session.

公开(公告)号：WO2009145828A3

公开(公告)日：2009-12-03

申请号：PCT/US2009/002003

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  BJORNSON, Keith ,  LEE, Harold ,  KORLACH, Jonas ,  TURNER, Stephen ,  BIBILLO, Arek ,  CHRISTIANS, Fred ,  EMIG, Robin ,  HE, Molly ,  PARK, Insil ,  JIA, Lei ,  FEDOROV, Andrei ,  LYLE, John

Inventor： BJORNSON, Keith ,  LEE, Harold ,  KORLACH, Jonas ,  TURNER, Stephen ,  BIBILLO, Arek ,  CHRISTIANS, Fred ,  EMIG, Robin ,  HE, Molly ,  PARK, Insil ,  JIA, Lei ,  FEDOROV, Andrei ,  LYLE, John

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.

公开(公告)号：WO2011040971A3

公开(公告)日：2011-11-03

申请号：PCT/US2010002659

申请日：2010-09-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

Inventor： CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

IPC: C12N9/12 ,  C12N15/54 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/6869 ,  C12Q2521/101

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

公开(公告)号：WO2009145820A3

公开(公告)日：2009-12-03

申请号：PCT/US2009/001974

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  CLARK, Sonya ,  BIBILLO, Arek ,  PELUSO, Paul ,  CHRISTIANS, Fred ,  HE, Molly ,  PARK, Insil ,  LEE, Harold ,  BJORNSON, Keith ,  JIA, Lei ,  EMIG, Robin

Inventor： CLARK, Sonya ,  BIBILLO, Arek ,  PELUSO, Paul ,  CHRISTIANS, Fred ,  HE, Molly ,  PARK, Insil ,  LEE, Harold ,  BJORNSON, Keith ,  JIA, Lei ,  EMIG, Robin

IPC: C12N9/12 ,  C12N15/54 ,  C12N15/10

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

公开(公告)号：WO2009145820A2

公开(公告)日：2009-12-03

申请号：PCT/US2009001974

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

Inventor： CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

IPC: C12N9/12 ,  C12N15/10 ,  C12N15/54

CPC classification number: C12N9/1252 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q2521/101

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract translation: 提供了包含修饰的重组聚合酶的组合物，其表现出小于其衍生自其的聚合酶的支化部分的支化部分，或对于磷酸酯标记的核苷酸类似物的支化部分小于约25％。 还提供了包含修饰的重组聚合酶的组合物，其表现出相对于亲本聚合酶具有增加的稳定性的闭合的聚合酶/ DNA复合物。 还提供了包含修饰的重组聚合酶的组合物，其相对于亲本聚合酶表现出降低的速率常数。 提供了产生具有上述表型的聚合酶的方法。 提供使用这种聚合酶制备DNA或顺序DNA模板的方法。

公开(公告)号：WO2011002731A2

公开(公告)日：2011-01-06

申请号：PCT/US2010040285

申请日：2010-06-29

Applicant: WAY OF H INC ,  LEE HAROLD ,  DANNENBERG ROGER

Inventor： LEE HAROLD ,  DANNENBERG ROGER

IPC: G10H7/00 ,  G10H1/34 ,  G10H3/18

CPC classification number: G09B15/00 ,  A63F11/0051 ,  A63F2300/69 ,  A63F2300/8047 ,  G09B5/065 ,  G09B15/023 ,  G09B15/04 ,  G10H1/0008 ,  G10H1/0016 ,  G10H1/368 ,  G10H3/186 ,  G10H2210/051 ,  G10H2210/066 ,  G10H2210/091 ,  G10H2220/005 ,  G10H2220/051 ,  G10H2220/056 ,  G10H2220/076 ,  G10H2220/126 ,  G10H2220/151

Abstract: A method includes receiving a user selection of a musical piece; providing performance cues to a user to perform musical events on a musical instrument, wherein the performance cues are synchronized to expert performance data of the musical piece; receiving audio data corresponding to musical events performed by the user on the musical instrument; detecting fundamental frequencies associated with the user-performed musical events; determining an extent to which the user-performed musical events have been correctly or incorrectly performed; providing real-time or near real-time audio feedback and/or visual feedback indicating the extent to which the user-performed musical events have been correctly or incorrectly performed; and using the expert performance data as real-time or near real-time audio and/or video feedback by controlling an output level of the expert performance data output to the user during a session.

Abstract translation: 一种方法包括接收用户对乐曲的选择; 向用户提供演奏提示以在乐器上执行音乐事件，其中所述演奏线索与所述乐曲的专家演奏数据同步; 接收对应于用户在乐器上执行的音乐事件的音频数据; 检测与用户执行的音乐事件相关联的基本频率; 确定用户执行的音乐事件已被正确地或不正确地执行的程度; 提供实时或接近实时音频反馈和/或视觉反馈，指示用户演奏的音乐事件已被正确地或不正确地执行的程度; 以及通过控制在会话期间向用户输出的专家性能数据的输出水平，将专家性能数据用作实时或接近实时的音频和/或视频反馈。

公开(公告)号：WO2009145828A2

公开(公告)日：2009-12-03

申请号：PCT/US2009002003

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  BJORNSON KEITH ,  LEE HAROLD ,  KORLACH JONAS ,  TURNER STEPHEN ,  BIBILLO AREK ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PARK INSIL ,  JIA LEI ,  FEDOROV ANDREI ,  LYLE JOHN

Inventor： BJORNSON KEITH ,  LEE HAROLD ,  KORLACH JONAS ,  TURNER STEPHEN ,  BIBILLO AREK ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PARK INSIL ,  JIA LEI ,  FEDOROV ANDREI ,  LYLE JOHN

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2527/125 ,  C12Q2527/119 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2527/137 ,  C12Q2521/101

Abstract: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.

Abstract translation: 用于核苷酸测序的组合物，试剂盒，方法和系统，其包括产生聚合酶反应，其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶，包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。

公开(公告)号：WO2004085201A3

公开(公告)日：2004-12-02

申请号：PCT/US2004008307

申请日：2004-03-18

Applicant: L & P PROPERTY MANAGEMENT CO ,  LIU JINTAO ,  LEE HAROLD

Inventor： LIU JINTAO ,  LEE HAROLD

IPC: A47C7/46 ,  A47C3/025 ,  F16D67/00

CPC classification number: B60N2/6671

Abstract: A clutch actuator has a housing with a load bearing element disposed within it. A force application element disposed within the housing applies force to the load bearing element. A stop assembly includes a retaining stopper between the load bearing element and the force application element. The stopper is dimensioned relative to a retainer and to the load bearing element to stop movement of said load bearing element relative to said retainer in the absence of a force application, and the stopper is dimensioned relative to said retainer and to said load bearing element to allow movement of said load bearing element relative to said retainer when force is applied against the load or when is force is released toward the load. One surface of the retainer, the stopper or the load bearing element is textured.

Abstract translation: 离合器致动器具有壳体，该壳体内具有承载元件。 布置在壳体内的施力元件将力施加到承载元件。 止动组件包括在承载元件和施力元件之间的保持止动件。 止动器相对于保持器和承载元件设定尺寸，以在不施加力的情况下阻止所述承载元件相对于所述保持器的移动，并且止动器相对于所述保持器和所述承载元件的尺寸为 允许所述承载元件相对于所述保持器在对所述负载施加力时或者当向着所述负载释放力时移动。 保持架，止动件或承载元件的一个表面具有纹理。

公开(公告)号：WO2004085201A2

公开(公告)日：2004-10-07

申请号：PCT/US2004/008307

申请日：2004-03-18

Applicant: L & P PROPERTY MANAGEMENT COMPANY ,  LIU, Jintao ,  LEE, Harold

Inventor： LIU, Jintao ,  LEE, Harold

IPC: B60R

CPC classification number: B60N2/6671

Abstract: A clutch actuator has a housing with a load bearing element disposed within it. A force application element disposed within the housing applies force to the load bearing element. A stop assembly includes a retaining stopper between the load bearing element and the force application element. The stopper is dimensioned relative to a retainer and to the load bearing element to stop movement of said load bearing element relative to said retainer in the absence of a force application, and the stopper is dimensioned relative to said retainer and to said load bearing element to allow movement of said load bearing element relative to said retainer when force is applied against the load or when is force is released toward the load. One surface of the retainer, the stopper or the load bearing element is textured.

Abstract translation: 离合器致动器具有设置在其内的承载元件的壳体。 设置在壳体内的力施加元件对承载元件施加力。 止动组件包括在承载元件和施力元件之间的保持止动件。 止动器相对于保持器和承载元件的尺寸设计成在没有施加力的情况下停止所述承载元件相对于所述保持器的运动，并且止动器相对于所述保持器和所述承载元件的尺寸设定为 允许所述承载元件相对于所述保持器的移动，当对所述负载施加力时或当力朝向负载释放时。 保持器，止动器或承载元件的一个表面是有纹理的。

公开(公告)号：WO2011040971A9

公开(公告)日：2011-04-07

申请号：PCT/US2010/002659

申请日：2010-09-30

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. ,  CLARK, Sonya ,  BIBILLO, Arek ,  PELUSO, Paul ,  CHRISTIANS, Fred ,  HE, Molly ,  PARK, Insil ,  LEE, Harold ,  BJORNSON, Keith ,  JIA, Lei ,  EMIG, Robin

Inventor： CLARK, Sonya ,  BIBILLO, Arek ,  PELUSO, Paul ,  CHRISTIANS, Fred ,  HE, Molly ,  PARK, Insil ,  LEE, Harold ,  BJORNSON, Keith ,  JIA, Lei ,  EMIG, Robin

IPC: C12N9/12 ,  C12N15/54 ,  C12Q1/68

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.