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    REAL-TIME REDOX SEQUENCING
    1.
    发明申请
    REAL-TIME REDOX SEQUENCING 审中-公开
    实时REDOX测序

    公开(公告)号：WO2013056241A3

    公开(公告)日：2013-06-20

    申请号：PCT/US2012060275

    申请日：2012-10-15

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA

    Inventor： KORLACH JONAS ,  TURNER STEPHEN ,  SUN LEI

    IPC: C12Q1/68 ,  C12Q1/48 ,  G01N27/26

    CPC classification number: C12Q1/6869 ,  B82Y15/00 ,  C12Q1/001 ,  C12Q2521/543 ,  C12Q2563/113 ,  C12Q2565/607 ,  C40B60/10 ,  G01N27/00 ,  G01N27/3277 ,  G01N27/3278 ,  C12Q2563/155

    Abstract: Real time redox sequencing methods, devices, and systems are described. Arrays of redox devices comprising one or two electrodes are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the electrode(s). A sequencing reaction mixture comprising nucleotide analogs comprising redox labels is introduced to the array of redox devices under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by electrochemically identifying the redox labels of the nucleotide analogs that are incorporated into the growing strand.

    Abstract translation: 描述了实时氧化还原测序方法，设备和系统。 使用包含一个或两个电极的氧化还原装置的阵列来提供关于靠近电极的聚合酶 - 模板复合物中的模板核酸的序列信息。 将包含氧化还原标记的核苷酸类似物的测序反应混合物在聚合酶介导的核酸合成的条件下引入到氧化还原装置阵列中。 通过电化学鉴定掺入生长链中的核苷酸类似物的氧化还原标记来确定核苷酸类似物的掺入时间序列。

    SUBSTRATES, SYSTEMS AND METHODS FOR ANALYZING MATERIALS
    6.
    发明申请
    SUBSTRATES, SYSTEMS AND METHODS FOR ANALYZING MATERIALS 审中-公开
    用于分析材料的基板，系统和方法

    公开(公告)号：WO2008028160A3

    公开(公告)日：2008-11-06

    申请号：PCT/US2007077454

    申请日：2007-08-31

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  LUNDQUIST PAUL ,  TURNER STEPHEN

    Inventor： LUNDQUIST PAUL ,  TURNER STEPHEN

    IPC: F21V9/16

    CPC classification number: G01N21/648 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7703 ,  G01N2021/7786 ,  G02B6/43

    Abstract: Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses.

    Abstract translation: 用于分析材料的衬底，系统和方法，其包括设置在衬底上或衬底内的波导阵列，使得从波导发出的消逝场照射设置在衬底表面上或其附近的材料，从而允许分析这些材料。 底物，系统和方法用于各种分析操作，包括核酸分析，包括杂交和测序分析，细胞分析和其他分子分析。

    ISOLATION OF POLYMERASE-NUCLEIC ACID COMPLEXES AND LOADING ONTO SUBSTRATES
    8.
    发明申请
    ISOLATION OF POLYMERASE-NUCLEIC ACID COMPLEXES AND LOADING ONTO SUBSTRATES 审中-公开
    分离聚合酶 - 核酸复合物并加载到基底上

    公开(公告)号：WO2012129242A3

    公开(公告)日：2012-12-27

    申请号：PCT/US2012029830

    申请日：2012-03-20

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  THANG PHAM ,  GRAY JEREMY ,  FEHR ADRIAN ,  BHAMIDIPATI ARUNASHREE ,  CHMURA AJ ,  TRAVERS KEVIN ,  OLIVARES ERIC

    Inventor： THANG PHAM ,  GRAY JEREMY ,  FEHR ADRIAN ,  BHAMIDIPATI ARUNASHREE ,  CHMURA AJ ,  TRAVERS KEVIN ,  OLIVARES ERIC

    IPC: C12Q1/48 ,  C12N15/11 ,  C12Q1/68 ,  G01N27/26 ,  G01N33/68

    CPC classification number: C12Q1/6874 ,  B01L9/523 ,  C12Q1/6869 ,  C40B60/14 ,  G01N33/54313 ,  G01N33/54353 ,  G01N2333/922 ,  C12Q2521/543 ,  C12Q2523/303 ,  C12Q2525/301

    Abstract: Compositions, methods and systems are provided for the isolation of polymerase-nucleic acid complexes. Complexes can be separated from free enzyme by using hook molecules to target single stranded regions on the nucleic acid. Active complexes can be isolated from mixtures having both active and inactive complexes by initiating nucleic acid synthesis so as to open up a portion of a double stranded region rendering that region single stranded. Hook molecules are targeted to bind the sequences that are thus exposed. The hook molecules bound to active polymerase-nucleic acid complex are isolated, and the active polymerase-nucleic acid complexes released. Also disclosed are compositions, devices, and methods for loading molecules-of-interest onto a substrate by contacting beads having molecules-of-interest attached to them with the substrate, for example by providing a field that brings the beads into proximity or contact with the substrate and moves the beads with respect to the substrate. Such molecules-of-interest can be deposited onto substrates for single-molecule analysis.

    Abstract translation: 提供了用于分离聚合酶 - 核酸复合物的组合物，方法和系统。 通过使用钩分子将复合物与游离酶分离以靶向核酸上的单链区域。 活性配合物可以通过引发核酸合成从具有活性和非活性复合物的混合物中分离出来，以便打开一部分双链区域，使该区域成为单链。 钩状分子被靶向以结合由此暴露的序列。 分离与活性聚合酶 - 核酸复合物结合的钩分子，释放活性聚合酶 - 核酸复合物。 还公开了通过使具有附着于其上的感兴趣分子的珠粒与底物接触将负载分子加载到底物上的组合物，装置和方法，例如通过提供使珠子接近或接触 所述基板并相对于所述基板移动所述珠。 这种感兴趣的分子可以沉积在底物上用于单分子分析。

    REAL-TIME SEQUENCING METHODS AND SYSTEMS
    10.
    发明申请
    REAL-TIME SEQUENCING METHODS AND SYSTEMS 审中-公开
    实时测序方法和系统

    公开(公告)号：WO2010129019A3

    公开(公告)日：2011-03-31

    申请号：PCT/US2010001236

    申请日：2010-04-26

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  KORLACH JONAS ,  TURNER STEPHEN ,  SCHADT ERIC

    Inventor： KORLACH JONAS ,  TURNER STEPHEN ,  SCHADT ERIC

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12Q1/6869 ,  C12Q1/702 ,  C12Q2565/518 ,  C12Q2547/101

    Abstract: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to either enhance or inhibit such reactions. In certain preferred embodiments, RNA templates are used in single-molecule real-time sequencing reactions.

    Abstract translation: 本发明一般涉及用于进行各种不同生物反应的单分子实时分析的组合物，方法和系统。 分析这些反应的能力提供了研究这些反应以及潜在鉴定影响这些反应的因素和/或方法的机会，例如增强或抑制这种反应。 在某些优选实施方案中，RNA模板用于单分子实时测序反应。

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