公开(公告)号：WO2012140507A1

公开(公告)日：2012-10-18

申请号：PCT/IB2012/000836

申请日：2012-04-10

Applicant: DR. REDDY'S LABORATORIES (EU) LIMITED ,  THE UNIVERSITY OF EDINBURGH ,  BAXTER, Scott ,  CAMPOPIANO, Dominic ,  HOLT-TIFFIN, Karen, Elizabeth

Inventor： BAXTER, Scott ,  CAMPOPIANO, Dominic ,  HOLT-TIFFIN, Karen, Elizabeth

IPC: C12N9/90

CPC classification number: C12P13/04 ,  C12N9/90 ,  C12P13/06 ,  C12P13/222 ,  C12Y501/01

Abstract: The present application relates to a mutated Amycoiatopsis sp . TS-1-60 NAAAR that shows improved activity of the enzyme compared with the wild type Amycoiatopsis sp . TS-1-60 NAAAR. The mutated NAAAR is almost five times more active than its wiid type counterpart. The present application also relates to the use of mutated Amycoiatopsis sp . TS-1 -60 NAAAR in the production of enantiomerically pure amino acid from its N-acyl derivative via dynamic kinetic resolution method.

Abstract translation: 本申请涉及突变的拟南芥（Amycoiatopsis sp。） 与野生型Amycoiatopsis sp相比，显示出酶活性提高的TS-1-60 NAAAR。 TS-1-60 NAAAR。 突变的NAAAR比其wiid型对应物几乎是活跃的五倍。 本申请还涉及使用突变的拟南芥（Amycoiatopsis sp。） TS-1 -60 NAAAR通过动态动力学拆分方法从其N-酰基衍生物生产对映体纯氨基酸。

公开(公告)号：WO2009091054A1

公开(公告)日：2009-07-23

申请号：PCT/JP2009/050601

申请日：2009-01-09

Applicant: 学校法人慶應義塾 ,  宮本憲二 ,  太田博道

Inventor： 宮本憲二 ,  太田博道

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12P41/00

CPC classification number: C12R1/13 ,  C12N9/0036 ,  C12P7/42 ,  C12P13/222

Abstract: 本発明は、Brevibacterium属微生物由来のpH安定性及び熱安定性に優れた新規NADHオキシダーゼをコードするDNAの提供を目的とし、本発明は以下の(a)又は(b)のBrevibacterium属微生物由来のNADHオキシダーゼをコードするDNAである。(a) 配列番号１８で表わされるアミノ酸配列を含むNADHオキシダーゼ(b) 配列番号１８で表わされるアミノ酸配列において１若しくは数個のアミノ酸が欠失、置換若しくは付加されたアミノ酸配列を含み、かつNADHオキシダーゼ活性を有するNADHオキシダーゼ

Abstract translation: 公开了编码来自属于短杆菌属的微生物的新型NADH氧化酶并具有优异的pH稳定性和热稳定性的DNA。 具体公开的是编码来自属于短杆菌属的微生物的NADH氧化酶的DNA，其选自下列NADH氧化酶（a）和（b）：（a）包含SEQ ID NO：18所示的氨基酸序列的NADH氧化酶 ; 和（b）NADH氧化酶，其包含在SEQ ID NO：18所示的氨基酸序列中具有缺失，取代或添加一个或几个氨基酸残基并具有NADH氧化酶活性的氨基酸序列。

公开(公告)号：WO2009014259A1

公开(公告)日：2009-01-29

申请号：PCT/JP2008/063722

申请日：2008-07-24

Applicant: AJINOMOTO CO., INC. ,  SAMSONOV, Valery, Vasilievich ,  GORBACHEVA, Lubov, Yurievna ,  AKHVERDIAN, Valeriy, Zavenovich ,  VOROSHILOVA, Elvira, Borisovna

Inventor： SAMSONOV, Valery, Vasilievich ,  GORBACHEVA, Lubov, Yurievna ,  AKHVERDIAN, Valeriy, Zavenovich ,  VOROSHILOVA, Elvira, Borisovna

IPC: C12P13/08 ,  C07K14/245

CPC classification number: C12P13/222 ,  C07K14/245 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/225 ,  C12P13/227 ,  C12P13/24

Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea , which has been modified to attenuate expression of the yncD gene.

Abstract translation: 本发明提供了使用肠杆菌科的细菌，特别是属于埃希氏杆菌属或泛菌属的细菌来生产L-氨基酸的方法，其已被修饰以减弱yncD基因的表达。

公开(公告)号：WO2008096837A1

公开(公告)日：2008-08-14

申请号：PCT/JP2008/052092

申请日：2008-02-04

Applicant: AJINOMOTO CO., INC. ,  SAMSONOV, Valery, Vasilievich ,  KAPLAN, Alla, Markovna ,  AKHVERDIAN, Valeriy, Zavenovich ,  VOROSHILOVA, Elvira, Borisovna ,  GUSYATINER, Mikhail, Markovich

Inventor： SAMSONOV, Valery, Vasilievich ,  KAPLAN, Alla, Markovna ,  AKHVERDIAN, Valeriy, Zavenovich ,  VOROSHILOVA, Elvira, Borisovna ,  GUSYATINER, Mikhail, Markovich

IPC: C12P13/08 ,  C07K14/245

CPC classification number: C07K14/245 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24

Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea , which has been modified to attenuate expression of the tolC gene.

Abstract translation: 本发明提供一种利用肠杆菌科的细菌，特别是属于埃希氏杆菌属或泛菌属的细菌来生产L-氨基酸的方法，该方法已被修饰以减弱tolC基因的表达。

公开(公告)号：WO2008010565A3

公开(公告)日：2008-05-29

申请号：PCT/JP2007064304

申请日：2007-07-12

Applicant: AJINOMOTO KK ,  PTITSYN LEONID ROMANOVICH ,  ALTMAN IRINA BORISOVNA ,  KOTLIAROVA VERONIKA ALEKSANDRO ,  MOKHOVA OLGA NIKOLAEVNA ,  YAMPOLSKAYA TATYANA ABRAMOVNA ,  KOZLOV YURY IVANOVICH ,  TERASHITA MASARU ,  USUDA YOSHIHIRO ,  MATSUI KAZUHIKO

Inventor： PTITSYN LEONID ROMANOVICH ,  ALTMAN IRINA BORISOVNA ,  KOTLIAROVA VERONIKA ALEKSANDRO ,  MOKHOVA OLGA NIKOLAEVNA ,  YAMPOLSKAYA TATYANA ABRAMOVNA ,  KOZLOV YURY IVANOVICH ,  TERASHITA MASARU ,  USUDA YOSHIHIRO ,  MATSUI KAZUHIKO

IPC: C12N9/04 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/14 ,  C12P13/22 ,  C12P13/24

CPC classification number: C12P13/08 ,  C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24 ,  C12Y101/01002

Abstract: A method for producing an L-amino acid is described, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of a wild-type alcohol dehydrogenase encoded by the adhE gene or a mutant alcohol dehydrogenase which is resistant to aerobic inactivation.

Abstract translation: 使用肠杆菌科细菌描述了生产L-氨基酸的方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸，L-色氨酸或L-谷氨酸 家族，其中所述细菌已被修饰以增强由adhE基因编码的野生型醇脱氢酶或耐有氧失活的突变醇脱氢酶的活性。

公开(公告)号：WO2006043730A1

公开(公告)日：2006-04-27

申请号：PCT/JP2005/019840

申请日：2005-10-21

Applicant: AJINOMOTO CO., INC. ,  RYBAK, Konstantin, Vyacheslavovich ,  SLIVINSKAYA, Ekaterina, Aleksandrovna ,  SAVRASOVA, Ekaterina, Alekseevna ,  AKHVERDIAN, Valeriy, Zavenovich ,  KLYACHKO, Elena, Vitalievna ,  MASHKO, Sergei, Vladimirovich ,  DOROSHENKO, Vera, Georgievna ,  AIRIKH, Larisa, Gotlibovna ,  LEONOVA, Tatyana, Viktorovba ,  GUSYATINER, Mikhail, Markovich ,  VOROSHILOVA, Elvira, Borisovna ,  KOZLOV, Yury, Ivanovich ,  HARA, Yoshihiko ,  UEDA, Takuji

Inventor： RYBAK, Konstantin, Vyacheslavovich ,  SLIVINSKAYA, Ekaterina, Aleksandrovna ,  SAVRASOVA, Ekaterina, Alekseevna ,  AKHVERDIAN, Valeriy, Zavenovich ,  KLYACHKO, Elena, Vitalievna ,  MASHKO, Sergei, Vladimirovich ,  DOROSHENKO, Vera, Georgievna ,  AIRIKH, Larisa, Gotlibovna ,  LEONOVA, Tatyana, Viktorovba ,  GUSYATINER, Mikhail, Markovich ,  VOROSHILOVA, Elvira, Borisovna ,  KOZLOV, Yury, Ivanovich ,  HARA, Yoshihiko ,  UEDA, Takuji

IPC: C07K14/245 ,  C12P13/04

CPC classification number: C12P13/08 ,  C12P13/10 ,  C12P13/14 ,  C12P13/222 ,  C12P13/24

Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

Abstract translation: 公开了使用肠杆菌科的细菌生产L-氨基酸的方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸或L-谷氨酸，其中细菌 已被修饰以增强D-木糖通透酶的活性。

公开(公告)号：WO2006038698A1

公开(公告)日：2006-04-13

申请号：PCT/JP2005/018699

申请日：2005-10-11

Applicant: 宇部興産株式会社 ,  古根川 唯泰 ,  宮田 博之 ,  山本 康仁

Inventor： 古根川 唯泰 ,  宮田 博之 ,  山本 康仁

IPC: C12P41/00 ,  C07C227/18 ,  C07C229/34 ,  C07B53/00

CPC classification number: C12P41/005 ,  C07C227/18 ,  C07C229/34 ,  C12P13/04 ,  C12P13/222

Abstract: A process for the production of optically active (S)- or (R) -ß-amino acids represented by the general formula (II): (II) [wherein R is optionally substituted, alkyl, alkenyl, alkynyl, cycloalkyl, aralkyl, aryl, or heteroaryl; and * represents an asymmetric carbon atom] and optically active (R)- or (S)-ß-amino acid esters represented by the general formula (III): (III) [wherein R and * are each as defined above; and R

Abstract translation: 用于制备由通式（II）表示的光学活性（S） - 或（R）-β-氨基酸的方法：（II）[其中R是任选取代的，烷基，烯基，炔基，环烷基，芳烷基， 芳基或杂芳基; 和*表示不对称碳原子]和由通式（III）表示的光学活性（R） - 或（S）-β-氨基酸酯：（III）[其中R和*各自如上所定义; 和R

公开(公告)号：WO2005106011A2

公开(公告)日：2005-11-10

申请号：PCT/US2005012615

申请日：2005-04-13

Applicant: SQUIBB BRISTOL MYERS CO ,  POLITINO MICHAEL ,  CADIN MATTHEW M ,  SKONEZNY PAUL M ,  CHEN JASON G

Inventor： POLITINO MICHAEL ,  CADIN MATTHEW M ,  SKONEZNY PAUL M ,  CHEN JASON G

IPC: C07H21/04 ,  C12N1/06 ,  C12N1/21 ,  C12N9/02 ,  C12N9/06 ,  C12N15/74 ,  C12P13/04 ,  C12P13/22 ,  C12P15/00 ,  C12P17/10 ,  C12P21/06

CPC classification number: C07D209/52 ,  C07H21/04 ,  C12N1/066 ,  C12N9/0008 ,  C12N9/0018 ,  C12P13/04 ,  C12P13/222 ,  C12P15/00 ,  C12P17/10 ,  C12Y102/02001

Abstract: A process for production of cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV is provided which employs a BOC-protected amine of the structure (3) prepared by subjecting an acid of the structure (1) to reduce amination by treating the acid with ammonium formate, nicotinamide adenine dinucleotide, dithiothreitol and partially purified phenylalanine dehydrogenase/formate dehydrogenase enzyme concentrate (PDH/FDH) and without isolating treating the resulting amine of the structure (2) with di-tert-butyl dicarbonate to form the BOC-protected amine.

Abstract translation: 提供了制备二肽基肽酶IV的环丙基稠合吡咯烷基抑制剂的方法，其采用BOC保护的结构式（3）的胺，其通过使结构（1）的酸通过用 甲酸铵，烟酰胺腺嘌呤二核苷酸，二硫苏糖醇和部分纯化的苯丙氨酸脱氢酶/甲酸脱氢酶浓缩物（PDH / FDH），并且不用二碳酸二叔丁酯处理得到的结构（2）的胺以形成BOC-保护的胺 。

公开(公告)号：WO2003018759A2

公开(公告)日：2003-03-06

申请号：PCT/US2002/026934

申请日：2002-08-23

Applicant: PCBU SERVICES, INC. ,  YOSHIDA, Roberta, K. ,  KOOTSTRA, Anna, B.

Inventor： YOSHIDA, Roberta, K. ,  KOOTSTRA, Anna, B.

IPC: C12N

CPC classification number: C12Y403/01005 ,  A61K38/00 ,  C12N9/88 ,  C12P13/222

Abstract: The present invention provides a Rhodotorula phenylalanine lyase polypeptide, polynucleotides that encode the polypeptide, and methods of obtaining and using these products. In particular the polypeptide can be employed for the production of phenylalanine, phenylalanine analogs, and optionally active unnatural amino acids having phenylalanine-like structures.

Abstract translation: 本发明提供了红酵母苯丙氨酸裂解酶多肽，编码多肽的多核苷酸，以及获得和使用这些产物的方法。 特别地，多肽可用于生产苯丙氨酸，苯丙氨酸类似物和任选具有苯丙氨酸样结构的活性非天然氨基酸。

公开(公告)号：WO1991004263A1

公开(公告)日：1991-04-04

申请号：PCT/US1990005369

申请日：1990-09-20

Applicant: THE NUTRASWEET COMPANY

Inventor： THE NUTRASWEET COMPANY ,  FOTHERINGHAM, Ian, Graham ,  NELMS, Jennifer

IPC: C07H21/04

CPC classification number: C12N15/52 ,  C12N9/88 ,  C12P13/222

Abstract: Disclosed are DNA sequences encoding deletion, substitution and/or addition analogs of the E. coli enzyme, chorismate mutase/prephenate dehydratase (CMPD). Preferred expression products include [des-Gln307, des-Ala308, des-Gly309, des-Ala310]CMPD; [Leu306]CMPD; [des-Thr?304, Lys305¿, des-Gln306]CMPD; and [Cys309]CMPD display enzymatic activity of the wild type enzyme but are more resistant to inhibition in the presence of phenylalanine.